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The map label for this gene is thiE [H]

Identifier: 146342550

GI number: 146342550

Start: 5914466

End: 5915074

Strand: Direct

Name: thiE [H]

Synonym: BRADO5712

Alternate gene names: 146342550

Gene position: 5914466-5915074 (Clockwise)

Preceding gene: 146342549

Following gene: 146342551

Centisome position: 79.32

GC content: 67.65

Gene sequence:

>609_bases
ATGCCGTATCCTGATCGCTTCTATCCCGTCGTCGACAGCCTCGCCTGGGTCGAGCGTCTCACCCGTCTCGGCGTCGGCAC
CGTGCAATTGCGCGCGAAGAATCTCGACGACGCCGCGGCGCTGCAGATCGTCACCGACGCGCTGGCCATCACCCGGGGCA
CCGCGACCAAGCTGATCGTCAACGACTACTGGCGCGCCGCGATCGCCGCCGGCGCCGCGCATGTCCATCTCGGCCAGGAG
GATCTGGCGGAGGCTGACGTCAAGGCGATCAAGGACGCCGGCCTGACGCTCGGCCTGTCGACGCATGACGATGCCGAACT
GGAGACTGCGCTCGCGGCGAAGCCCGACTACGTCGCGTTGGGGCCGATCTTCCCGACCACGCTCAAGGCGATGCGCTTCG
CGCCGCAGGGCATCCCGAAGATCAGCGTGTGGAAGCAGCGCGTCGGCAGCATTCCGCTGGTCGCCATCGGCGGCATCAAA
CTGGAGCAGGCCGCGGAGATTTTTGCCGCCGGCGCTGATTCCATCGCGGTTGTCTCCGACGTCACCCAGAACGCAGATCC
CGACGCCCGCGTCCGCGCCTGGCTCGACGCGACCAAGGAGCCGGCATGA

Upstream 100 bases:

>100_bases
CTCGGAGTCGAAGCCGGCCGCACCGCCTACGAGGCCGGTCTGATGGACGCCCGCGATTTCGCCTCGCCCTCCACCCCTGT
CGTTGGGACTCCGTTCTGGC

Downstream 100 bases:

>100_bases
TGCCCCACTCGCTCCACCGTCATTGCGAGCGAAGCGAAGCAATCCAGGGCCGCGCGTGCCGCCCTGGATTGCTTCGTCGC
TACGCTCCTCGCAATGACGC

Product: thiamine phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MPYPDRFYPVVDSLAWVERLTRLGVGTVQLRAKNLDDAAALQIVTDALAITRGTATKLIVNDYWRAAIAAGAAHVHLGQE
DLAEADVKAIKDAGLTLGLSTHDDAELETALAAKPDYVALGPIFPTTLKAMRFAPQGIPKISVWKQRVGSIPLVAIGGIK
LEQAAEIFAAGADSIAVVSDVTQNADPDARVRAWLDATKEPA

Sequences:

>Translated_202_residues
MPYPDRFYPVVDSLAWVERLTRLGVGTVQLRAKNLDDAAALQIVTDALAITRGTATKLIVNDYWRAAIAAGAAHVHLGQE
DLAEADVKAIKDAGLTLGLSTHDDAELETALAAKPDYVALGPIFPTTLKAMRFAPQGIPKISVWKQRVGSIPLVAIGGIK
LEQAAEIFAAGADSIAVVSDVTQNADPDARVRAWLDATKEPA
>Mature_201_residues
PYPDRFYPVVDSLAWVERLTRLGVGTVQLRAKNLDDAAALQIVTDALAITRGTATKLIVNDYWRAAIAAGAAHVHLGQED
LAEADVKAIKDAGLTLGLSTHDDAELETALAAKPDYVALGPIFPTTLKAMRFAPQGIPKISVWKQRVGSIPLVAIGGIKL
EQAAEIFAAGADSIAVVSDVTQNADPDARVRAWLDATKEPA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=186, Percent_Identity=50.5376344086022, Blast_Score=178, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325042, Length=214, Percent_Identity=28.5046728971963, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21447; Mature: 21316

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYPDRFYPVVDSLAWVERLTRLGVGTVQLRAKNLDDAAALQIVTDALAITRGTATKLIV
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHH
NDYWRAAIAAGAAHVHLGQEDLAEADVKAIKDAGLTLGLSTHDDAELETALAAKPDYVAL
HHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCEEEE
GPIFPTTLKAMRFAPQGIPKISVWKQRVGSIPLVAIGGIKLEQAAEIFAAGADSIAVVSD
CCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCEEHHHHHHHHHCCCCCCHHHHH
VTQNADPDARVRAWLDATKEPA
HHCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
PYPDRFYPVVDSLAWVERLTRLGVGTVQLRAKNLDDAAALQIVTDALAITRGTATKLIV
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHH
NDYWRAAIAAGAAHVHLGQEDLAEADVKAIKDAGLTLGLSTHDDAELETALAAKPDYVAL
HHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCEEEE
GPIFPTTLKAMRFAPQGIPKISVWKQRVGSIPLVAIGGIKLEQAAEIFAAGADSIAVVSD
CCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCEEHHHHHHHHHCCCCCCHHHHH
VTQNADPDARVRAWLDATKEPA
HHCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]