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The map label for this gene is thiC [H]

Identifier: 146342551

GI number: 146342551

Start: 5915213

End: 5917114

Strand: Direct

Name: thiC [H]

Synonym: BRADO5713

Alternate gene names: 146342551

Gene position: 5915213-5917114 (Clockwise)

Preceding gene: 146342550

Following gene: 146342552

Centisome position: 79.33

GC content: 65.09

Gene sequence:

>1902_bases
ATGAACATCCGCTCCAATCCCGACACCACCCTGCCCGCCGTCACCACTGGCCCGCTGCCGTCGTCGCGCAAGATCTACGC
TGTGCCGGACTCCGCCCCCGATCTGCGCGTGCCCTTGCGCGAGATCATCCTGTCGGAAGCAGCCAGCGAGCCGAACCTGC
CGGTCTACGACACCTCCGGCCCCTACACCGACCCGTCCGTCACCATCGACGTCAACGCGGGTCTGCCCCGCAACCGCATC
GCCTGGGTGCTCGAGCGCGGCGGCGTCGAGGAATATGTCGGCCGCGACATCAAGCCGGAGGACAATGGCAATGTCGGCGC
CGACAAGGCGGCGAAGGCGTTCACCGCGCACCACAAGCCGCTGCGCGGACTCGACGGCCACAAGATCACCCAGCTCGAAT
TTGCCCGCGCCGGCATCGTCACCAAGGAGATGATCTACGTCGCCGAGCGCGAGAATCTCGGACGTAAAGTTCAGCTCGAG
CGCGCCGAGGCCGCGCTCGCCGACGGCGAGAGCTTTGGCGCGTCGGTCCCCGCCTTCATCACCCCGGAATTCGTCCGCGA
CGAGATCGCGCGCGGCCGCGCCATCATCCCCTGCAACATCAACCACGCCGAGCTGGAGCCGATGATCATCGGCCGCAACT
TCCTCACCAAGATCAACGCCAATATCGGCAACTCGGCGGTGACCTCCTCAGTGGAAGAGGAAGTGGACAAGATGGTGTGG
GCGATCCGCTGGGGCGCCGACACGGTGATGGACCTCTCGACCGGCCGCAACATCCACACCACCCGCGAATGGATCCTGCG
CAACTCGCCGGTGCCGATCGGCACCGTGCCGATCTACCAGGCGCTGGAGAAGTGCAACGGCGATCCGGTGGCGCTGACCT
GGGAGCTCTACAAGGACACGCTGATCGAGCAGTGCGAGCAGGGCGTCGACTACTTCACCATCCACGCCGGCGTGCGGCTG
CAATACATCCACCTCACCGCGAGCCGCGTCACCGGCATCGTCAGCCGCGGCGGCTCGATCATGGCGAAGTGGTGCCTCGC
GCATCACAAGGAGAGCTTCCTGTACACGCACTTTGATGAGATCTGCGACATCATGCGCAAGTATGACGTCTCGTTCTCGC
TCGGAGACGGCCTGCGCCCCGGCTCGATCGCCGATGCCAACGATCGCGCCCAGTTCGCTGAGCTGGAGACCCTGGGCGAG
CTGACCAAGATCGCCTGGGACAAGGGCTGCCAGGTCATGATCGAGGGCCCCGGCCACGTGCCGATGCACAAGATCAAGAT
CAACATGGACAAGCAGCTCAAGGAGTGCGGCGAGGCGCCGTTCTACACGCTCGGGCCGCTGACCACCGACATCGCGCCGG
GCTACGACCACATCACGTCCGGCATCGGTGCGGCCATGATCGGCTGGTTCGGCTGCGCCATGCTGTGCTACGTGACGCCG
AAGGAGCATCTCGGCCTGCCCGATCGCAACGACGTCAAGACCGGCGTCATCACCTACAAGATCGCCGCCCACGCCGCCGA
CCTCGCCAAGGGCCATCCCGCAGCGCAGCTACGCGACGACGCGCTGTCGCGCGCCCGCTTCGAGTTCCGCTGGACCGACC
AGTTCAACCTCGGCCTCGACCCGGACACCGCGAAGAGCTTCCACGACGAGACCCTGCCGAAGGAAGCCCACAAGGTCGCG
CATTTCTGCTCGATGTGCGGCCCCAAATTCTGCTCGATGAAGATCACCCAGGACGTCCGCGACTACGCCGCGACCTTGAA
CGATCCGGCGACGGTCGGCATGACCATCTCCGGCACCATCGAGGACGGCATGGCCCAAATGAGCGCCAAGTTCAAGGAAA
TGGGCGGCAGCGTGTACCTGGATGCGGAGAAGGTGAAGGAGAGCAACAAGGCGTTGTCCTGA

Upstream 100 bases:

>100_bases
GCAATCCAGGGCCGCGCGTGCCGCCCTGGATTGCTTCGTCGCTACGCTCCTCGCAATGACGCTCCTATTCACTCCGGCAA
CGACAATTAAGGAGGCCCCC

Downstream 100 bases:

>100_bases
CCTCTCGCGCATCGCTCTCCATCGTCATGGCCGGGCTTGACCCGGCCATCCATCAAGCCGGGCGCAAGCCCGGCTTTTTT
GTTTGATGATTAGACCATTC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MNIRSNPDTTLPAVTTGPLPSSRKIYAVPDSAPDLRVPLREIILSEAASEPNLPVYDTSGPYTDPSVTIDVNAGLPRNRI
AWVLERGGVEEYVGRDIKPEDNGNVGADKAAKAFTAHHKPLRGLDGHKITQLEFARAGIVTKEMIYVAERENLGRKVQLE
RAEAALADGESFGASVPAFITPEFVRDEIARGRAIIPCNINHAELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCNGDPVALTWELYKDTLIEQCEQGVDYFTIHAGVRL
QYIHLTASRVTGIVSRGGSIMAKWCLAHHKESFLYTHFDEICDIMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGE
LTKIAWDKGCQVMIEGPGHVPMHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
KEHLGLPDRNDVKTGVITYKIAAHAADLAKGHPAAQLRDDALSRARFEFRWTDQFNLGLDPDTAKSFHDETLPKEAHKVA
HFCSMCGPKFCSMKITQDVRDYAATLNDPATVGMTISGTIEDGMAQMSAKFKEMGGSVYLDAEKVKESNKALS

Sequences:

>Translated_633_residues
MNIRSNPDTTLPAVTTGPLPSSRKIYAVPDSAPDLRVPLREIILSEAASEPNLPVYDTSGPYTDPSVTIDVNAGLPRNRI
AWVLERGGVEEYVGRDIKPEDNGNVGADKAAKAFTAHHKPLRGLDGHKITQLEFARAGIVTKEMIYVAERENLGRKVQLE
RAEAALADGESFGASVPAFITPEFVRDEIARGRAIIPCNINHAELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCNGDPVALTWELYKDTLIEQCEQGVDYFTIHAGVRL
QYIHLTASRVTGIVSRGGSIMAKWCLAHHKESFLYTHFDEICDIMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGE
LTKIAWDKGCQVMIEGPGHVPMHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
KEHLGLPDRNDVKTGVITYKIAAHAADLAKGHPAAQLRDDALSRARFEFRWTDQFNLGLDPDTAKSFHDETLPKEAHKVA
HFCSMCGPKFCSMKITQDVRDYAATLNDPATVGMTISGTIEDGMAQMSAKFKEMGGSVYLDAEKVKESNKALS
>Mature_633_residues
MNIRSNPDTTLPAVTTGPLPSSRKIYAVPDSAPDLRVPLREIILSEAASEPNLPVYDTSGPYTDPSVTIDVNAGLPRNRI
AWVLERGGVEEYVGRDIKPEDNGNVGADKAAKAFTAHHKPLRGLDGHKITQLEFARAGIVTKEMIYVAERENLGRKVQLE
RAEAALADGESFGASVPAFITPEFVRDEIARGRAIIPCNINHAELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCNGDPVALTWELYKDTLIEQCEQGVDYFTIHAGVRL
QYIHLTASRVTGIVSRGGSIMAKWCLAHHKESFLYTHFDEICDIMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGE
LTKIAWDKGCQVMIEGPGHVPMHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
KEHLGLPDRNDVKTGVITYKIAAHAADLAKGHPAAQLRDDALSRARFEFRWTDQFNLGLDPDTAKSFHDETLPKEAHKVA
HFCSMCGPKFCSMKITQDVRDYAATLNDPATVGMTISGTIEDGMAQMSAKFKEMGGSVYLDAEKVKESNKALS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=619, Percent_Identity=65.4281098546042, Blast_Score=820, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69530; Mature: 69530

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRSNPDTTLPAVTTGPLPSSRKIYAVPDSAPDLRVPLREIILSEAASEPNLPVYDTSG
CCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCC
PYTDPSVTIDVNAGLPRNRIAWVLERGGVEEYVGRDIKPEDNGNVGADKAAKAFTAHHKP
CCCCCCEEEEECCCCCCCHHHHEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCC
LRGLDGHKITQLEFARAGIVTKEMIYVAERENLGRKVQLERAEAALADGESFGASVPAFI
CCCCCCCEEEEEHHHHHCCHHHHHEEEEHHCCCCCEEEHHHHHHHHHCCHHCCCCCCCCC
TPEFVRDEIARGRAIIPCNINHAELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
CHHHHHHHHHCCCEEEEECCCHHHCCEEEECCHHHHEECCCCCCHHHHHHHHHHHHHHHE
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCNGDPVALTWELYKDT
EEEECCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHH
LIEQCEQGVDYFTIHAGVRLQYIHLTASRVTGIVSRGGSIMAKWCLAHHKESFLYTHFDE
HHHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
ICDIMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGELTKIAWDKGCQVMIEGPGHV
HHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PMHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
CEEEEEECCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECC
KEHLGLPDRNDVKTGVITYKIAAHAADLAKGHPAAQLRDDALSRARFEFRWTDQFNLGLD
HHHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHHCEEEEEECCCCCCCCC
PDTAKSFHDETLPKEAHKVAHFCSMCGPKFCSMKITQDVRDYAATLNDPATVGMTISGTI
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCEEEEEEECCH
EDGMAQMSAKFKEMGGSVYLDAEKVKESNKALS
HHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCCC
>Mature Secondary Structure
MNIRSNPDTTLPAVTTGPLPSSRKIYAVPDSAPDLRVPLREIILSEAASEPNLPVYDTSG
CCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCC
PYTDPSVTIDVNAGLPRNRIAWVLERGGVEEYVGRDIKPEDNGNVGADKAAKAFTAHHKP
CCCCCCEEEEECCCCCCCHHHHEECCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCC
LRGLDGHKITQLEFARAGIVTKEMIYVAERENLGRKVQLERAEAALADGESFGASVPAFI
CCCCCCCEEEEEHHHHHCCHHHHHEEEEHHCCCCCEEEHHHHHHHHHCCHHCCCCCCCCC
TPEFVRDEIARGRAIIPCNINHAELEPMIIGRNFLTKINANIGNSAVTSSVEEEVDKMVW
CHHHHHHHHHCCCEEEEECCCHHHCCEEEECCHHHHEECCCCCCHHHHHHHHHHHHHHHE
AIRWGADTVMDLSTGRNIHTTREWILRNSPVPIGTVPIYQALEKCNGDPVALTWELYKDT
EEEECCCCEEECCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHH
LIEQCEQGVDYFTIHAGVRLQYIHLTASRVTGIVSRGGSIMAKWCLAHHKESFLYTHFDE
HHHHHHCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
ICDIMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGELTKIAWDKGCQVMIEGPGHV
HHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PMHKIKINMDKQLKECGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTP
CEEEEEECCHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECC
KEHLGLPDRNDVKTGVITYKIAAHAADLAKGHPAAQLRDDALSRARFEFRWTDQFNLGLD
HHHCCCCCCCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHHHCEEEEEECCCCCCCCC
PDTAKSFHDETLPKEAHKVAHFCSMCGPKFCSMKITQDVRDYAATLNDPATVGMTISGTI
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCEEEEEEECCH
EDGMAQMSAKFKEMGGSVYLDAEKVKESNKALS
HHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]