| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
Click here to switch to the map view.
The map label for this gene is murF [H]
Identifier: 146342502
GI number: 146342502
Start: 5857729
End: 5859105
Strand: Reverse
Name: murF [H]
Synonym: BRADO5663
Alternate gene names: 146342502
Gene position: 5859105-5857729 (Counterclockwise)
Preceding gene: 146342503
Following gene: 146342501
Centisome position: 78.58
GC content: 68.7
Gene sequence:
>1377_bases ATGACGAAGGCGCTGTGGACGGTGGCCGAGGCGGCGCGCGCGCTCGGTCTCGCCGGCGACTATCCTGAGACCGCGATCGA TTTCGTCACCCAGGACAGCCGCGCGGTGAAGCCCGGTAGCCTGTTCGTCGCGCTGAGCGGCACGCCGAGCGGCGGCTTCG TATCGAGCTTCGCCTCCAGCCGCGACGGCTGGGAGTTTGCCGCCAATGCCGAGCAGGCTGGCGCGGTGGCGATGATCGTG CCGCATCGGATCGAGGGCGTCACCGTGCCGCAGCTGGTGGTGACGGACACGCTGATCGATGGGCTATGGGCGCTGGCGCG CGCCGCACGGTCGCGCTTTCATGGTCCCGTGATCGGATTGACCGGCAGTGCCGGCAAGACCTCGACCAAGGAGTTCATCG CGGCCTATCCCGGGGCATCAGCGAGCCCGTCGAGCTTCAACAATTTCTGGGGCGTGCCGCTGACGCTGTGCAATGCCGAT CCCTCCGCCTCGGTCTGGGTGGTCGAGATGGGCATGAACCAGAAAGGTGAGATCGCGCGGCTGACCGAACTGTCGCAGCC GACCGTCGCCCTTGTCGTCAACGTTCAGCCCGTGCATCTGGAAAAGCTCGGCAGCCTCGAGGCGATCCGGCAGGAGAAGG TCTCGATTGCGCGCGGCCTGCCGTCAGGCGGGATCCTGGTGCTGCCGGCCGATCTCGCGGCTCCTGAATGGTCCGGCAAG ACCATTCGCTTCGGCGGCGATGCCGAGGTGCGCGAGCTGTCGCACACCGCCCGCGGCGAGAGCTGGGACGTGACGGCCGA CATTGCAGGCTGCAGCGTCAACTTCGCGCTCACGCCCGGCGCGCCGCACCGGCTGCATAATGCCCTCGCGGCGCTTGCCT CTGTGGCCGCCGCCGGCCTCGATCCGGCGCTGTTGGCACCGGAGCTCGGCGAGGTCGGCATCATGACCGGCCGCGGCGTC GAGCAGACCGTCGGCGACATCGTGCTGATCGACGACAGCTTCAACGGCAATCCGGCCAGCATGGCGGCGGCGCTCGATGG GCTGCAGGCCCGGCCGGTGCGGGGGCGGCGGATCGCCGTGATCGGGGATATGCTGGAGCTTGGCGACGAGGCGCCGGCCT ATCACGCCAAGATGGTCGGCCATCTCGCCGGCATCGACGGCGTCTTTTGTGTCGGGCCGCTGATGCGTCACCTGTTCGAG GCCTTGCCGCAAGGCAGACGGCTCGGCTGGCACGAGGACCCCGTCACGCTCGACCCGAAGCAGGTCGCGGCGCTGCTCGC GCCCGGTGACGTCGTGGTCGTCAAGGGCAGCAAGAAGATCTTCTGGGTCAACAAGTTCGTGGCCAAGCTCGCCGCCGCGC TGCAGGGCAGCGCGTGA
Upstream 100 bases:
>100_bases TCGCCGGCAAGGGCCACGAGACCGGACAGATCATCGGCGATACGGTTCTGCATTTCAGCGATCACGAAGCGGTCGCGGAC GCACTGGCAACGAGGGCATC
Downstream 100 bases:
>100_bases AATCGGGCGGTATCGCGGCGCGGCAGAGGGCGATTTTCCACTGGTGGTCGGTGCAAGGGCGGGCGGAACAAACCGTCGGG ATGAGTCGCACCGGGCTCCG
Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 458; Mature: 457
Protein sequence:
>458_residues MTKALWTVAEAARALGLAGDYPETAIDFVTQDSRAVKPGSLFVALSGTPSGGFVSSFASSRDGWEFAANAEQAGAVAMIV PHRIEGVTVPQLVVTDTLIDGLWALARAARSRFHGPVIGLTGSAGKTSTKEFIAAYPGASASPSSFNNFWGVPLTLCNAD PSASVWVVEMGMNQKGEIARLTELSQPTVALVVNVQPVHLEKLGSLEAIRQEKVSIARGLPSGGILVLPADLAAPEWSGK TIRFGGDAEVRELSHTARGESWDVTADIAGCSVNFALTPGAPHRLHNALAALASVAAAGLDPALLAPELGEVGIMTGRGV EQTVGDIVLIDDSFNGNPASMAAALDGLQARPVRGRRIAVIGDMLELGDEAPAYHAKMVGHLAGIDGVFCVGPLMRHLFE ALPQGRRLGWHEDPVTLDPKQVAALLAPGDVVVVKGSKKIFWVNKFVAKLAAALQGSA
Sequences:
>Translated_458_residues MTKALWTVAEAARALGLAGDYPETAIDFVTQDSRAVKPGSLFVALSGTPSGGFVSSFASSRDGWEFAANAEQAGAVAMIV PHRIEGVTVPQLVVTDTLIDGLWALARAARSRFHGPVIGLTGSAGKTSTKEFIAAYPGASASPSSFNNFWGVPLTLCNAD PSASVWVVEMGMNQKGEIARLTELSQPTVALVVNVQPVHLEKLGSLEAIRQEKVSIARGLPSGGILVLPADLAAPEWSGK TIRFGGDAEVRELSHTARGESWDVTADIAGCSVNFALTPGAPHRLHNALAALASVAAAGLDPALLAPELGEVGIMTGRGV EQTVGDIVLIDDSFNGNPASMAAALDGLQARPVRGRRIAVIGDMLELGDEAPAYHAKMVGHLAGIDGVFCVGPLMRHLFE ALPQGRRLGWHEDPVTLDPKQVAALLAPGDVVVVKGSKKIFWVNKFVAKLAAALQGSA >Mature_457_residues TKALWTVAEAARALGLAGDYPETAIDFVTQDSRAVKPGSLFVALSGTPSGGFVSSFASSRDGWEFAANAEQAGAVAMIVP HRIEGVTVPQLVVTDTLIDGLWALARAARSRFHGPVIGLTGSAGKTSTKEFIAAYPGASASPSSFNNFWGVPLTLCNADP SASVWVVEMGMNQKGEIARLTELSQPTVALVVNVQPVHLEKLGSLEAIRQEKVSIARGLPSGGILVLPADLAAPEWSGKT IRFGGDAEVRELSHTARGESWDVTADIAGCSVNFALTPGAPHRLHNALAALASVAAAGLDPALLAPELGEVGIMTGRGVE QTVGDIVLIDDSFNGNPASMAAALDGLQARPVRGRRIAVIGDMLELGDEAPAYHAKMVGHLAGIDGVFCVGPLMRHLFEA LPQGRRLGWHEDPVTLDPKQVAALLAPGDVVVVKGSKKIFWVNKFVAKLAAALQGSA
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=436, Percent_Identity=33.4862385321101, Blast_Score=147, Evalue=1e-36,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 47700; Mature: 47569
Theoretical pI: Translated: 6.01; Mature: 6.01
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTKALWTVAEAARALGLAGDYPETAIDFVTQDSRAVKPGSLFVALSGTPSGGFVSSFASS CCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHCC RDGWEFAANAEQAGAVAMIVPHRIEGVTVPQLVVTDTLIDGLWALARAARSRFHGPVIGL CCCCEEECCCHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE TGSAGKTSTKEFIAAYPGASASPSSFNNFWGVPLTLCNADPSASVWVVEMGMNQKGEIAR ECCCCCCCHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHH LTELSQPTVALVVNVQPVHLEKLGSLEAIRQEKVSIARGLPSGGILVLPADLAAPEWSGK HHHCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC TIRFGGDAEVRELSHTARGESWDVTADIAGCSVNFALTPGAPHRLHNALAALASVAAAGL EEEECCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCC DPALLAPELGEVGIMTGRGVEQTVGDIVLIDDSFNGNPASMAAALDGLQARPVRGRRIAV CHHHHCCCCCCEEEEECCCHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCEEEE IGDMLELGDEAPAYHAKMVGHLAGIDGVFCVGPLMRHLFEALPQGRRLGWHEDPVTLDPK EEHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECHH QVAALLAPGDVVVVKGSKKIFWVNKFVAKLAAALQGSA HHHHEECCCCEEEEECCCEEEEHHHHHHHHHHHHCCCC >Mature Secondary Structure TKALWTVAEAARALGLAGDYPETAIDFVTQDSRAVKPGSLFVALSGTPSGGFVSSFASS CCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHCC RDGWEFAANAEQAGAVAMIVPHRIEGVTVPQLVVTDTLIDGLWALARAARSRFHGPVIGL CCCCEEECCCHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE TGSAGKTSTKEFIAAYPGASASPSSFNNFWGVPLTLCNADPSASVWVVEMGMNQKGEIAR ECCCCCCCHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCHH LTELSQPTVALVVNVQPVHLEKLGSLEAIRQEKVSIARGLPSGGILVLPADLAAPEWSGK HHHCCCCEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC TIRFGGDAEVRELSHTARGESWDVTADIAGCSVNFALTPGAPHRLHNALAALASVAAAGL EEEECCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHCCC DPALLAPELGEVGIMTGRGVEQTVGDIVLIDDSFNGNPASMAAALDGLQARPVRGRRIAV CHHHHCCCCCCEEEEECCCHHHHHCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCEEEE IGDMLELGDEAPAYHAKMVGHLAGIDGVFCVGPLMRHLFEALPQGRRLGWHEDPVTLDPK EEHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECHH QVAALLAPGDVVVVKGSKKIFWVNKFVAKLAAALQGSA HHHHEECCCCEEEEECCCEEEEHHHHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 7894708; 8905231 [H]