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The map label for this gene is murE [H]

Identifier: 146342503

GI number: 146342503

Start: 5859102

End: 5860562

Strand: Reverse

Name: murE [H]

Synonym: BRADO5664

Alternate gene names: 146342503

Gene position: 5860562-5859102 (Counterclockwise)

Preceding gene: 146342504

Following gene: 146342502

Centisome position: 78.6

GC content: 67.69

Gene sequence:

>1461_bases
ATGAAACTTCACGACCTCCTCGGCGATCAGGCGGGGCTTGACCCGCGCGTCGCCGCCGTCGAAGTCTCCGGCCTTGCCGC
GGACAGCCGCGCTGTCCGGCCCGGAGACCTGTTCTTCGCGCTGTCGGGAACCAAGACCGATGGCACTCGCTTCATCGCGG
CTGCGGTCGCTGCGGGGGCGATCGCGGTGGCCGGCCACGAAGCCCCGGCCGGTGATCTGCCCGTTCCCTTCGTCTCGCTG
CCAAATCCGCGACGTGCTCTGGCGCTTGCTGCATCGCGCGTCTTCCCCCGGCAGCCGGCAACGATCGCGGCGGTGACCGG
AACCAGCGGCAAGACGTCGGTCGCAGCCTTCACGCGGCAGATCTGGCAGAGCCTCGGCCACAACGCGGCGAGTATCGGCA
CGGTCGGATTGATTTCGGACAAGCGCACCGTCTACGGCTCGCTGACCACGCCCGACCCGATTGCGCTGCATCGGCAGATC
GACGAGATCACCCAGGACGGCGTGACGCACCTCGCTTTCGAAGCGTCGTCGCATGGGCTCGATCAATACAGGCTGGACGG
CGTCAGGGTCTCGGCCGGCGGGTTCACCAACCTGTCGCGCGACCACATGGATTACCACCCCGATCTCGTCCATTATCTCA
ATGCCAAGCTGCGGCTGTTTCGCGACCTTGTGCCGCCGGGCGGGCCGGCGGTGATTTCGGCGGATCATGAGTGCTCGGCA
GAAGTGATTGCCGCGGCTGATGCGCGCGGGCTCCGGCTGATGACGGTGGGGACGCATGGCAATGGCGCAGGGGCGGGCAT
CCGTCTCGTGGCCGCCGCCGTCGATGGCTTTGCACAGCAGCTCGAGCTCCAGCATTGCGGCCGGATCACCTTCCTCCGCC
TGCCGCTGGTCGGCGCGTTTCAGATCGAGAATGCGCTGGTGGCCGCGGGCCTTGCCATCGGCACCGGCAGCGATCCGCAG
GCGGTGTTCGAGGCTCTGGAAAAGCTCGAAGGCGCCAAGGGCCGGCTCGAACTGGTCGGTGAGCACAACGGCGCGCCCAT
CTTCATCGACTACGCTCACAAGCCGGATGCGCTGGCGAAGGCGCTGCACGCCTTGCGGCCGTATGCCCAGAGAAAGCTCG
TGGTCGTGTTCGGCGCCGGCGGCGACCGGGACAGCGGCAAGCGGCCGCTGATGGGCGAGATCGCCGCTGCCGGCGCTGAT
CGCGTTATCGTCACCGACGACAATCCCCGGAGCGAGAATCCCGCTGCGATCCGCGCCGCGATCCTGGCCGCCGCACCTGG
TGCGCGCGAGATCGGCGATCGCGCCGAGGCGATCCGGGTGGCGATCAGCGAGCTTCAGCCGGGGGATGCGCTGGTCATCG
CCGGCAAGGGCCACGAGACCGGACAGATCATCGGCGATACGGTTCTGCATTTCAGCGATCACGAAGCGGTCGCGGACGCA
CTGGCAACGAGGGCATCATGA

Upstream 100 bases:

>100_bases
TTCTTGCGGCATCGCGGCTGACGCAGTAAGCCGTCCGCGAATCGCATGGCTTTGGAGCCGCCGCCGCCCGGGGCGGGGCC
AGGACCGGTACGGGACCAGG

Downstream 100 bases:

>100_bases
CGAAGGCGCTGTGGACGGTGGCCGAGGCGGCGCGCGCGCTCGGTCTCGCCGGCGACTATCCTGAGACCGCGATCGATTTC
GTCACCCAGGACAGCCGCGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGAIAVAGHEAPAGDLPVPFVSL
PNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQIWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQI
DEITQDGVTHLAFEASSHGLDQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA
EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAFQIENALVAAGLAIGTGSDPQ
AVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAKALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGAD
RVIVTDDNPRSENPAAIRAAILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA
LATRAS

Sequences:

>Translated_486_residues
MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGAIAVAGHEAPAGDLPVPFVSL
PNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQIWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQI
DEITQDGVTHLAFEASSHGLDQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA
EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAFQIENALVAAGLAIGTGSDPQ
AVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAKALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGAD
RVIVTDDNPRSENPAAIRAAILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA
LATRAS
>Mature_486_residues
MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGAIAVAGHEAPAGDLPVPFVSL
PNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQIWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQI
DEITQDGVTHLAFEASSHGLDQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA
EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAFQIENALVAAGLAIGTGSDPQ
AVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAKALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGAD
RVIVTDDNPRSENPAAIRAAILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA
LATRAS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=36.7556468172485, Blast_Score=230, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50452; Mature: 50452

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGA
CCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCE
IAVAGHEAPAGDLPVPFVSLPNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQ
EEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH
IWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQIDEITQDGVTHLAFEASSHGL
HHHHHCCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
DQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA
CCEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAF
CEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEE
QIENALVAAGLAIGTGSDPQAVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAK
EHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHH
ALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGADRVIVTDDNPRSENPAAIRAA
HHHHHCHHHCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
ILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCEEEEECCHHHHHHH
LATRAS
HHHCCC
>Mature Secondary Structure
MKLHDLLGDQAGLDPRVAAVEVSGLAADSRAVRPGDLFFALSGTKTDGTRFIAAAVAAGA
CCCHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCE
IAVAGHEAPAGDLPVPFVSLPNPRRALALAASRVFPRQPATIAAVTGTSGKTSVAAFTRQ
EEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH
IWQSLGHNAASIGTVGLISDKRTVYGSLTTPDPIALHRQIDEITQDGVTHLAFEASSHGL
HHHHHCCCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCC
DQYRLDGVRVSAGGFTNLSRDHMDYHPDLVHYLNAKLRLFRDLVPPGGPAVISADHECSA
CCEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
EVIAAADARGLRLMTVGTHGNGAGAGIRLVAAAVDGFAQQLELQHCGRITFLRLPLVGAF
CEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEE
QIENALVAAGLAIGTGSDPQAVFEALEKLEGAKGRLELVGEHNGAPIFIDYAHKPDALAK
EHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHHH
ALHALRPYAQRKLVVVFGAGGDRDSGKRPLMGEIAAAGADRVIVTDDNPRSENPAAIRAA
HHHHHCHHHCCEEEEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
ILAAAPGAREIGDRAEAIRVAISELQPGDALVIAGKGHETGQIIGDTVLHFSDHEAVADA
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEECCEEEEECCHHHHHHH
LATRAS
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]