Click here to switch to the map view.

The map label for this gene is leuD [H]

Identifier: 146342361

GI number: 146342361

Start: 5700899

End: 5701516

Strand: Direct

Name: leuD [H]

Synonym: BRADO5517

Alternate gene names: 146342361

Gene position: 5700899-5701516 (Clockwise)

Preceding gene: 146342360

Following gene: 146342363

Centisome position: 76.45

GC content: 65.7

Gene sequence:

>618_bases
ATGACGCCGTTCGACATCCTCACGACATCGGCCTGCGCGCTGCCGCTCGCCAGCATCGACACGGATCAGCTGATCCCCGC
ACGCTTCATGAAGCGCTCGCGCGCCGACGGCTATGGACAGTTTCTCCTCCACGACATGCGCTTCGACGAGACCGGCGCGG
CACGCTCCGACTTTCCGCTCAACGCGGCCTCGGCGGAGGGCGCCGAAGTGCTGGTGACGCGGCGGAATTTCGGCGCCGGC
TCGTCGCGCGAGGCGGCGGTCTATGCGCTGGTCGATTTCGGCTTCCGCTGCGTGATCGCGCCGAGCTTCGGCGACATCTT
CGCCTCGAACGCGGTGAACAACGGATTGTTGCCGGCGAAGGTCAGCGAAGCCGATGCGGAAGAGATCCTGGCGCTGCTGC
AGACGGCGGGAACCACCATCACGGTCGATCTCCACGACGCGCTGATACGGCTGGGCAACCGTACGTTTACCTTCTCGATC
GATCCGATCTGGCGCACCAAGCTGCTCAACGGCTGGGACGATCTCGACCTCACGGCCTCGCTGACCGGAGATATCGTCCG
GTTCGAGCAACAAGACAGCGCCGTGCGGCCCTGGGTGCAGCTGCCTGGGCGCAGCTGA

Upstream 100 bases:

>100_bases
GCCCCGGCGCGCGCACGCATCTGATGTCGCCGGCGATGGTCGCGGCCGCCGCGGTCACCGGCCATCTCACCGACGTGCGG
CCGCTGCTGAGGAGCGATGC

Downstream 100 bases:

>100_bases
AGGACAGCAGCGATTGAGCTGATCGACCTCCACAGAAAGAAGCCGCCCGCACGTAGCGTGCAGGCGGCCCAAGTCCAGGG
AGGAAACGCCCCAAGAAGGG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTPFDILTTSACALPLASIDTDQLIPARFMKRSRADGYGQFLLHDMRFDETGAARSDFPLNAASAEGAEVLVTRRNFGAG
SSREAAVYALVDFGFRCVIAPSFGDIFASNAVNNGLLPAKVSEADAEEILALLQTAGTTITVDLHDALIRLGNRTFTFSI
DPIWRTKLLNGWDDLDLTASLTGDIVRFEQQDSAVRPWVQLPGRS

Sequences:

>Translated_205_residues
MTPFDILTTSACALPLASIDTDQLIPARFMKRSRADGYGQFLLHDMRFDETGAARSDFPLNAASAEGAEVLVTRRNFGAG
SSREAAVYALVDFGFRCVIAPSFGDIFASNAVNNGLLPAKVSEADAEEILALLQTAGTTITVDLHDALIRLGNRTFTFSI
DPIWRTKLLNGWDDLDLTASLTGDIVRFEQQDSAVRPWVQLPGRS
>Mature_204_residues
TPFDILTTSACALPLASIDTDQLIPARFMKRSRADGYGQFLLHDMRFDETGAARSDFPLNAASAEGAEVLVTRRNFGAGS
SREAAVYALVDFGFRCVIAPSFGDIFASNAVNNGLLPAKVSEADAEEILALLQTAGTTITVDLHDALIRLGNRTFTFSID
PIWRTKLLNGWDDLDLTASLTGDIVRFEQQDSAVRPWVQLPGRS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=40.7216494845361, Blast_Score=146, Evalue=9e-37,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=38.265306122449, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22284; Mature: 22153

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFDILTTSACALPLASIDTDQLIPARFMKRSRADGYGQFLLHDMRFDETGAARSDFPL
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCC
NAASAEGAEVLVTRRNFGAGSSREAAVYALVDFGFRCVIAPSFGDIFASNAVNNGLLPAK
CCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCC
VSEADAEEILALLQTAGTTITVDLHDALIRLGNRTFTFSIDPIWRTKLLNGWDDLDLTAS
CCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCEEEEEEECCHHHHHHCCCCCCCCEEEE
LTGDIVRFEQQDSAVRPWVQLPGRS
CCCCEEEEECCCCCCCCCEECCCCC
>Mature Secondary Structure 
TPFDILTTSACALPLASIDTDQLIPARFMKRSRADGYGQFLLHDMRFDETGAARSDFPL
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCC
NAASAEGAEVLVTRRNFGAGSSREAAVYALVDFGFRCVIAPSFGDIFASNAVNNGLLPAK
CCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCEEEEECCCHHHHHHHCCCCCCCCCCC
VSEADAEEILALLQTAGTTITVDLHDALIRLGNRTFTFSIDPIWRTKLLNGWDDLDLTAS
CCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHCCEEEEEEECCHHHHHHCCCCCCCCEEEE
LTGDIVRFEQQDSAVRPWVQLPGRS
CCCCEEEEECCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA