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The map label for this gene is leuC [H]

Identifier: 146342360

GI number: 146342360

Start: 5699484

End: 5700902

Strand: Direct

Name: leuC [H]

Synonym: BRADO5516

Alternate gene names: 146342360

Gene position: 5699484-5700902 (Clockwise)

Preceding gene: 146342359

Following gene: 146342361

Centisome position: 76.44

GC content: 68.5

Gene sequence:

>1419_bases
ATGACGGGAACAGGGCAGCCGCGGACGCTGTTCGACAAGGTCTGGGATGGGCATGTGGTGACGCGCCGCGAGGACGGCGC
GGAGCTGTTGTTCATCGACCGCCACCTCTTGCATGAGGGCTCGTTCCATGCCTTCAACAAGCTCAAGGACAAGCGCGCCA
AGGTCCGGCGGCCGGATCTCACCATCGGTGTCGCGGATCATTATGTGCCGACTCGTACGCGCGCGCTGAGCGAGGTCGCG
CCCGACATCGCCGGCATGATCCGTCAACTCGACGACAATTGCCGCGCCAACGCCATCCGCATCTTCGGCTTCGACGATCC
CCGCCAGGGCATCGTGCACGTCGTCGGTCCCGAGCAGGGCCTGACGCTGCCCGGACTCACCATGGTCTGCGGCGACAGCC
ACACCTCGACGCATGGCGCGTTCGGCGCGCTGGCCTTCGGCATCGGCGCCTCCGAGGTCGCGCATGTGCTGCTGACGCAG
ACGCTGTGGCAGAAGAAGCCAAGGCGGATGCGCATCACGGTGGACGGCGCGACCGCGCCGGGGATCACCGCCAAGGACAT
CGCGCTCTCGATCATCGCAAGGATCAGCGCCGACGGCGCGCAGGGGCACGCGGTCGAGTATACGGGATCCGCGATCCGCG
CGCTGACGATGGAGGGCCGGCTGACGCTCTGTAATCTCTCGATCGAGAGCGGCGCGCGATGCGGCATGATTGCGCCTGAC
GAGACGACGTTCGCCTACGTGAGGGGACGGCCACTCGCGCCCGCCGGCGAGGTGCTCGACGGCGCCATCGCGGCCTGGCG
AGAGCTGACGAGCGATGCCGACGCGGCGTTCGACCGCGCGGTCCTGCTCGATGGCAAGGACATCGCGCCGACTGTGACCT
GGGGCATCAGCCCAGAGGATGCGCTGCCGATCGGCGCGTCGGTGCCTGATCCCGCAGCCATGGACGATGCGTCGCGCGCG
AGCCATGTCCGCGAGGCGCTCGACTACATGGGCCTCGCGCCCGGCCAATCACTCGAGACGGTCAGGATCGACCGCGTGTT
CATCGGCTCCTGCACCAACAGCCGCATCGAGGATCTGCGCGCTGCGGCAAGCGTCCTCGCTGGCCGTAAGGCGCGCGTGC
CCGGTCTCGTCTCGCCGGGCTCGCACCTTGTCAAGCAGCAGGCCGAGCAGGAAGGCCTCGACCGCATCTTCGTCGATGCC
GGCCTGGACTGGGTTGGTTCAGGCTGCTCGATGTGCGTCGGCATGAACGGCGATCTCGTTCCAGCGGGCGAGCGCTGCGC
CTCCACCACCAACCGCAACTTCAAGGGCCGCCAGGGCCCCGGCGCGCGCACGCATCTGATGTCGCCGGCGATGGTCGCGG
CCGCCGCGGTCACCGGCCATCTCACCGACGTGCGGCCGCTGCTGAGGAGCGATGCATGA

Upstream 100 bases:

>100_bases
TATCAGGGTCACATGGCCGCGCGCGCCCCCTTCCCTGAAGCGGCCGAACATCCGACATGACTGGTGCGCTGATGATGCGA
TCGACTGAGGACATTCGGGA

Downstream 100 bases:

>100_bases
CGCCGTTCGACATCCTCACGACATCGGCCTGCGCGCTGCCGCTCGCCAGCATCGACACGGATCAGCTGATCCCCGCACGC
TTCATGAAGCGCTCGCGCGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTGTGQPRTLFDKVWDGHVVTRREDGAELLFIDRHLLHEGSFHAFNKLKDKRAKVRRPDLTIGVADHYVPTRTRALSEVA
PDIAGMIRQLDDNCRANAIRIFGFDDPRQGIVHVVGPEQGLTLPGLTMVCGDSHTSTHGAFGALAFGIGASEVAHVLLTQ
TLWQKKPRRMRITVDGATAPGITAKDIALSIIARISADGAQGHAVEYTGSAIRALTMEGRLTLCNLSIESGARCGMIAPD
ETTFAYVRGRPLAPAGEVLDGAIAAWRELTSDADAAFDRAVLLDGKDIAPTVTWGISPEDALPIGASVPDPAAMDDASRA
SHVREALDYMGLAPGQSLETVRIDRVFIGSCTNSRIEDLRAAASVLAGRKARVPGLVSPGSHLVKQQAEQEGLDRIFVDA
GLDWVGSGCSMCVGMNGDLVPAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDVRPLLRSDA

Sequences:

>Translated_472_residues
MTGTGQPRTLFDKVWDGHVVTRREDGAELLFIDRHLLHEGSFHAFNKLKDKRAKVRRPDLTIGVADHYVPTRTRALSEVA
PDIAGMIRQLDDNCRANAIRIFGFDDPRQGIVHVVGPEQGLTLPGLTMVCGDSHTSTHGAFGALAFGIGASEVAHVLLTQ
TLWQKKPRRMRITVDGATAPGITAKDIALSIIARISADGAQGHAVEYTGSAIRALTMEGRLTLCNLSIESGARCGMIAPD
ETTFAYVRGRPLAPAGEVLDGAIAAWRELTSDADAAFDRAVLLDGKDIAPTVTWGISPEDALPIGASVPDPAAMDDASRA
SHVREALDYMGLAPGQSLETVRIDRVFIGSCTNSRIEDLRAAASVLAGRKARVPGLVSPGSHLVKQQAEQEGLDRIFVDA
GLDWVGSGCSMCVGMNGDLVPAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDVRPLLRSDA
>Mature_471_residues
TGTGQPRTLFDKVWDGHVVTRREDGAELLFIDRHLLHEGSFHAFNKLKDKRAKVRRPDLTIGVADHYVPTRTRALSEVAP
DIAGMIRQLDDNCRANAIRIFGFDDPRQGIVHVVGPEQGLTLPGLTMVCGDSHTSTHGAFGALAFGIGASEVAHVLLTQT
LWQKKPRRMRITVDGATAPGITAKDIALSIIARISADGAQGHAVEYTGSAIRALTMEGRLTLCNLSIESGARCGMIAPDE
TTFAYVRGRPLAPAGEVLDGAIAAWRELTSDADAAFDRAVLLDGKDIAPTVTWGISPEDALPIGASVPDPAAMDDASRAS
HVREALDYMGLAPGQSLETVRIDRVFIGSCTNSRIEDLRAAASVLAGRKARVPGLVSPGSHLVKQQAEQEGLDRIFVDAG
LDWVGSGCSMCVGMNGDLVPAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDVRPLLRSDA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI8659555, Length=398, Percent_Identity=27.1356783919598, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=26.3020833333333, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI4501867, Length=371, Percent_Identity=25.6064690026954, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=54.4276457883369, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=29.1208791208791, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI2367097, Length=350, Percent_Identity=26.2857142857143, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=377, Percent_Identity=28.9124668435013, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=377, Percent_Identity=28.9124668435013, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=28.3018867924528, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=408, Percent_Identity=25.4901960784314, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=53.6842105263158, Blast_Score=514, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6320440, Length=349, Percent_Identity=28.080229226361, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=369, Percent_Identity=27.9132791327913, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=379, Percent_Identity=27.9683377308707, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI281365315, Length=378, Percent_Identity=26.1904761904762, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=378, Percent_Identity=26.1904761904762, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076999, Length=378, Percent_Identity=26.1904761904762, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=378, Percent_Identity=27.7777777777778, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645686, Length=369, Percent_Identity=27.10027100271, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17137564, Length=381, Percent_Identity=26.509186351706, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50154; Mature: 50022

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTGQPRTLFDKVWDGHVVTRREDGAELLFIDRHLLHEGSFHAFNKLKDKRAKVRRPDL
CCCCCCCHHHHHHHCCCEEEEECCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHCCCCCE
TIGVADHYVPTRTRALSEVAPDIAGMIRQLDDNCRANAIRIFGFDDPRQGIVHVVGPEQG
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCC
LTLPGLTMVCGDSHTSTHGAFGALAFGIGASEVAHVLLTQTLWQKKPRRMRITVDGATAP
CCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GITAKDIALSIIARISADGAQGHAVEYTGSAIRALTMEGRLTLCNLSIESGARCGMIAPD
CCCHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCC
ETTFAYVRGRPLAPAGEVLDGAIAAWRELTSDADAAFDRAVLLDGKDIAPTVTWGISPED
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCEEEECCCCCCCEEEECCCCCC
ALPIGASVPDPAAMDDASRASHVREALDYMGLAPGQSLETVRIDRVFIGSCTNSRIEDLR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHH
AAASVLAGRKARVPGLVSPGSHLVKQQAEQEGLDRIFVDAGLDWVGSGCSMCVGMNGDLV
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHCCCCEEEECCCCCCC
PAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDVRPLLRSDA
CCCHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGTGQPRTLFDKVWDGHVVTRREDGAELLFIDRHLLHEGSFHAFNKLKDKRAKVRRPDL
CCCCCCHHHHHHHCCCEEEEECCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHCCCCCE
TIGVADHYVPTRTRALSEVAPDIAGMIRQLDDNCRANAIRIFGFDDPRQGIVHVVGPEQG
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCC
LTLPGLTMVCGDSHTSTHGAFGALAFGIGASEVAHVLLTQTLWQKKPRRMRITVDGATAP
CCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GITAKDIALSIIARISADGAQGHAVEYTGSAIRALTMEGRLTLCNLSIESGARCGMIAPD
CCCHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCC
ETTFAYVRGRPLAPAGEVLDGAIAAWRELTSDADAAFDRAVLLDGKDIAPTVTWGISPED
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCEEEECCCCCCCEEEECCCCCC
ALPIGASVPDPAAMDDASRASHVREALDYMGLAPGQSLETVRIDRVFIGSCTNSRIEDLR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHH
AAASVLAGRKARVPGLVSPGSHLVKQQAEQEGLDRIFVDAGLDWVGSGCSMCVGMNGDLV
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHCCCCEEEECCCCCCC
PAGERCASTTNRNFKGRQGPGARTHLMSPAMVAAAAVTGHLTDVRPLLRSDA
CCCHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA