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The map label for this gene is hyuB

Identifier: 146342351

GI number: 146342351

Start: 5689077

End: 5690810

Strand: Reverse

Name: hyuB

Synonym: BRADO5507

Alternate gene names: NA

Gene position: 5690810-5689077 (Counterclockwise)

Preceding gene: 146342352

Following gene: 146342350

Centisome position: 76.32

GC content: 67.07

Gene sequence:

>1734_bases
ATGACCAACATGGATCCGATCACGCTCGCGGTGCTGAAGGGGCGGCTGGAGCAGATCGCCGACGAAATGGATGCGACGTT
GTACCGCTCCGCGTTCAACCCGACGATCGCGGAGGCGCGCGACGCCTGCCACGGCATCTATCACGCGACGACCGGCGACA
CGCTGGTGCAGGGGCAGGGCGGTCTGCCGATCTTCGTCGGCGCGATGGCTTTCGCCGTGCGCGCCGTCATCGCCAAGGTC
GAGAAGGAGGGCGGGCTCGAGGAGGGCGACACCTTTATCTTCAACGATCCCTATGACGGCGGCACCCATCTTAACGATTT
CCGCCTGGTGCGACCGGTGTTCCGCGACAACAGGGTGTTCTGCTGGATTGCCTCGGTCGGCCACTGGCTCGACATTGGCG
GCAACGTCGCCGGCGGCTACAATCCGAAGGCGGTCGAGAGTTTTCAGGAAGGCATGCGTATCCCGCCGGTCAAGCTGATC
GCGGCGGGACGCATGAACCACGACATCCTCGGCATCATCGAAGCCAATTCGCGCGTGCCGATGTCGAACTGGGGCGACCT
CAACGGCCAGCTCAACGCGCTCGATCTCGGCGAGATCAGACTGCGCGCGCTGCTCGACGATTACGGCGACAAAACCGTCT
CCAATGCGTTCGAGGCGTTCTCTGATCGCGCCGAGACCCTGATGCGGGCGGCGATCGCCTCACTGCCGGACGGCACCTAT
TCATTCGAGGACGTGCTCGACAATGACGGCATCGTCGACGAACCGCTGACCGTCGCGCTCGATGTCACCATTGCCGGCGA
CACGCTGACGCTCGATTTCACCCGCTCGTCGGCGGCCTGCAAGGGCCCGATCAACATCTCGCGCTCGACCACGATCGCGG
CCTGCTACGTCGCGCTGAAGCATGTGTTCACCGAGGTGCCGGCCAATGCCGGCTGCCTGCGGCCGATCTCGTTCGACATC
ACCGACGGCTCGCTGCTGGCGGCCGGGCCGCCGAAGCCGGTCGCGGGCTATACCGAGACCATCCTGCGCCTGATCGGCGT
CGTGCTCGGCGCGCTCGCCAAGGCCGATCCGTCGCGCGCCACCGCCGCGCCGTTCGGCACCATCAATGCGCTGTCGATCG
CGGGCCATCGCAAGGACAACAGCCGCTTCGTGATGTTCTCGTTCTTCGGCGGCGGCCTCGGCGGCAATCCCGCGACCGAC
GGCCTCAACCACGCCAACAACCCGATCTCGACCGCGACCATTCCGCCCGTGGAAATCTTGGAAGCAGCCTATCCCGTCAT
GTTCACGCAATGGGCGTTGCGCCCGGATTCCGCCGGTGCCGGCACCCATCGCGGCGGCGTCGGCGCTGTCTATGAGGTCG
AACTGCTGGCCGATGCCGATGTCGCGCTGCTCGGCGAGCGCGGCAAAGCGGCGCCGTTCGGCGTCGCCGGCGGCGGCAGC
GCCGCGCTCAATCATTTCGCCTGGCAGACGGAGCAGGGCGAGGCGAGCCCGCCGATGGTGTCCAAGATCACCGACCTGCA
TTTGAAGGCCGGCAAGCGCGTGCGGCTGGAGACACCCGGAGGCGGCGGCTGGGGTCCGCCGGAGGCGCGGCCCGTTGCTG
AGGTCGCGCACGACGTCGCGCTCGGCTTCGTCTCGGCGGCGCAGGCGCGTGACGTCTACAAGGTTGCCGTGAAGGCAGAC
GGCGCGGTCGATGAAGCCGCGACGCACACGCTCCGTGCGGGAGGTGCGGCATGA

Upstream 100 bases:

>100_bases
ACCGCATGCTCGATTTCGCCGGTCTCAACGCGATGCTCGGCACCGCCGATATCCTGGCGAACGGTGCCCGTTACGCAGCA
GATGACGAGGGAACGCGGGC

Downstream 100 bases:

>100_bases
GCAAGCTCGGCACCATCGTCGGCGTCGATGTCGGCGGCACCTTCACGGACCTGTTCTACTTCGACGAGGCGAAGCGCAGC
TTCCGCACCAACAAGGTGCC

Product: N-methylhydantoinase B

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MTNMDPITLAVLKGRLEQIADEMDATLYRSAFNPTIAEARDACHGIYHATTGDTLVQGQGGLPIFVGAMAFAVRAVIAKV
EKEGGLEEGDTFIFNDPYDGGTHLNDFRLVRPVFRDNRVFCWIASVGHWLDIGGNVAGGYNPKAVESFQEGMRIPPVKLI
AAGRMNHDILGIIEANSRVPMSNWGDLNGQLNALDLGEIRLRALLDDYGDKTVSNAFEAFSDRAETLMRAAIASLPDGTY
SFEDVLDNDGIVDEPLTVALDVTIAGDTLTLDFTRSSAACKGPINISRSTTIAACYVALKHVFTEVPANAGCLRPISFDI
TDGSLLAAGPPKPVAGYTETILRLIGVVLGALAKADPSRATAAPFGTINALSIAGHRKDNSRFVMFSFFGGGLGGNPATD
GLNHANNPISTATIPPVEILEAAYPVMFTQWALRPDSAGAGTHRGGVGAVYEVELLADADVALLGERGKAAPFGVAGGGS
AALNHFAWQTEQGEASPPMVSKITDLHLKAGKRVRLETPGGGGWGPPEARPVAEVAHDVALGFVSAAQARDVYKVAVKAD
GAVDEAATHTLRAGGAA

Sequences:

>Translated_577_residues
MTNMDPITLAVLKGRLEQIADEMDATLYRSAFNPTIAEARDACHGIYHATTGDTLVQGQGGLPIFVGAMAFAVRAVIAKV
EKEGGLEEGDTFIFNDPYDGGTHLNDFRLVRPVFRDNRVFCWIASVGHWLDIGGNVAGGYNPKAVESFQEGMRIPPVKLI
AAGRMNHDILGIIEANSRVPMSNWGDLNGQLNALDLGEIRLRALLDDYGDKTVSNAFEAFSDRAETLMRAAIASLPDGTY
SFEDVLDNDGIVDEPLTVALDVTIAGDTLTLDFTRSSAACKGPINISRSTTIAACYVALKHVFTEVPANAGCLRPISFDI
TDGSLLAAGPPKPVAGYTETILRLIGVVLGALAKADPSRATAAPFGTINALSIAGHRKDNSRFVMFSFFGGGLGGNPATD
GLNHANNPISTATIPPVEILEAAYPVMFTQWALRPDSAGAGTHRGGVGAVYEVELLADADVALLGERGKAAPFGVAGGGS
AALNHFAWQTEQGEASPPMVSKITDLHLKAGKRVRLETPGGGGWGPPEARPVAEVAHDVALGFVSAAQARDVYKVAVKAD
GAVDEAATHTLRAGGAA
>Mature_576_residues
TNMDPITLAVLKGRLEQIADEMDATLYRSAFNPTIAEARDACHGIYHATTGDTLVQGQGGLPIFVGAMAFAVRAVIAKVE
KEGGLEEGDTFIFNDPYDGGTHLNDFRLVRPVFRDNRVFCWIASVGHWLDIGGNVAGGYNPKAVESFQEGMRIPPVKLIA
AGRMNHDILGIIEANSRVPMSNWGDLNGQLNALDLGEIRLRALLDDYGDKTVSNAFEAFSDRAETLMRAAIASLPDGTYS
FEDVLDNDGIVDEPLTVALDVTIAGDTLTLDFTRSSAACKGPINISRSTTIAACYVALKHVFTEVPANAGCLRPISFDIT
DGSLLAAGPPKPVAGYTETILRLIGVVLGALAKADPSRATAAPFGTINALSIAGHRKDNSRFVMFSFFGGGLGGNPATDG
LNHANNPISTATIPPVEILEAAYPVMFTQWALRPDSAGAGTHRGGVGAVYEVELLADADVALLGERGKAAPFGVAGGGSA
ALNHFAWQTEQGEASPPMVSKITDLHLKAGKRVRLETPGGGGWGPPEARPVAEVAHDVALGFVSAAQARDVYKVAVKADG
AVDEAATHTLRAGGAA

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=551, Percent_Identity=28.6751361161525, Blast_Score=159, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI133901900, Length=551, Percent_Identity=29.4010889292196, Blast_Score=182, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI133901902, Length=491, Percent_Identity=29.3279022403259, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322634, Length=554, Percent_Identity=25.9927797833935, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45550492, Length=546, Percent_Identity=28.021978021978, Blast_Score=152, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 60569; Mature: 60438

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNMDPITLAVLKGRLEQIADEMDATLYRSAFNPTIAEARDACHGIYHATTGDTLVQGQG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCEEEECCC
GLPIFVGAMAFAVRAVIAKVEKEGGLEEGDTFIFNDPYDGGTHLNDFRLVRPVFRDNRVF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEE
CWIASVGHWLDIGGNVAGGYNPKAVESFQEGMRIPPVKLIAAGRMNHDILGIIEANSRVP
EEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCC
MSNWGDLNGQLNALDLGEIRLRALLDDYGDKTVSNAFEAFSDRAETLMRAAIASLPDGTY
CCCCCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SFEDVLDNDGIVDEPLTVALDVTIAGDTLTLDFTRSSAACKGPINISRSTTIAACYVALK
CHHHHHCCCCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
HVFTEVPANAGCLRPISFDITDGSLLAAGPPKPVAGYTETILRLIGVVLGALAKADPSRA
HHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
TAAPFGTINALSIAGHRKDNSRFVMFSFFGGGLGGNPATDGLNHANNPISTATIPPVEIL
CCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
EAAYPVMFTQWALRPDSAGAGTHRGGVGAVYEVELLADADVALLGERGKAAPFGVAGGGS
HHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCH
AALNHFAWQTEQGEASPPMVSKITDLHLKAGKRVRLETPGGGGWGPPEARPVAEVAHDVA
HHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
LGFVSAAQARDVYKVAVKADGAVDEAATHTLRAGGAA
HHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TNMDPITLAVLKGRLEQIADEMDATLYRSAFNPTIAEARDACHGIYHATTGDTLVQGQG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEECCCCEEEECCC
GLPIFVGAMAFAVRAVIAKVEKEGGLEEGDTFIFNDPYDGGTHLNDFRLVRPVFRDNRVF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEE
CWIASVGHWLDIGGNVAGGYNPKAVESFQEGMRIPPVKLIAAGRMNHDILGIIEANSRVP
EEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCC
MSNWGDLNGQLNALDLGEIRLRALLDDYGDKTVSNAFEAFSDRAETLMRAAIASLPDGTY
CCCCCCCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SFEDVLDNDGIVDEPLTVALDVTIAGDTLTLDFTRSSAACKGPINISRSTTIAACYVALK
CHHHHHCCCCCCCCCEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
HVFTEVPANAGCLRPISFDITDGSLLAAGPPKPVAGYTETILRLIGVVLGALAKADPSRA
HHHHHCCCCCCCCCCEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
TAAPFGTINALSIAGHRKDNSRFVMFSFFGGGLGGNPATDGLNHANNPISTATIPPVEIL
CCCCCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
EAAYPVMFTQWALRPDSAGAGTHRGGVGAVYEVELLADADVALLGERGKAAPFGVAGGGS
HHHHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCCH
AALNHFAWQTEQGEASPPMVSKITDLHLKAGKRVRLETPGGGGWGPPEARPVAEVAHDVA
HHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
LGFVSAAQARDVYKVAVKADGAVDEAATHTLRAGGAA
HHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]