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The map label for this gene is hyuA [H]

Identifier: 146342350

GI number: 146342350

Start: 5687020

End: 5689080

Strand: Reverse

Name: hyuA [H]

Synonym: BRADO5506

Alternate gene names: 146342350

Gene position: 5689080-5687020 (Counterclockwise)

Preceding gene: 146342351

Following gene: 146342349

Centisome position: 76.3

GC content: 69.09

Gene sequence:

>2061_bases
ATGAGCAAGCTCGGCACCATCGTCGGCGTCGATGTCGGCGGCACCTTCACGGACCTGTTCTACTTCGACGAGGCGAAGCG
CAGCTTCCGCACCAACAAGGTGCCGTCGAACCGCGGCGACGAAGCGGTCGGCTTCCTCGAAGGCCTGCAAGGTTTCGGCG
CGGTCTCGCATCTCACCTCGATCGTGCACGGCACGACCGTCGGCACCAATGCGCTGTTGGAGCGCAAGGGCGCGCGCATC
GGCCTGATCACGACGCGTGGCTTCCGCGACGTGCTGGAGATGCGCCGCCGCGACCGCCGCAACACCTGGGGCCTGTGGGG
CGACTTCGTGCCCGTCGTCGAGCGCGACATGCGCGCCGAGGTCGACGAGCGCGTGCTGTCGGACGGCACCATCCGCACGG
CCGTTGACGTTGCGCAGGTCGAGACGACCGCGCGCGAGCTGTTGGCAAAGGGCGCGCAGGCGCTGGCGATCGTGTTCGTC
AACGCCTTCGCCAATCCCGACAACGAGCGCCGCGCGCTCGAAGCCGTGCAGGCGATCTGGCCGAACGACAATGTCGCCTG
CTCCAGCCAGATCCTGCCGGAGATCCGCGAGTTCGAGCGGACCTCGACGACCGCGCTCAACGCCTATCTGCAGCCGGTGG
TCGGCTCCTATTTGCAGAAGCTGCAGAACGCGCTGGCGGGTGAAGCGTTCGACGGCCGCTTCCACATCGTGCAGTCCAAT
GGCGGCGTGATGTCCACGGACGCGGCGCGGCGCTTCCCGATCCGCACCGCGCTGTCGGGCCCGGCGGCTGGCGTGATCGC
CGCGGCGGAGATCGCCAAGGCGGCCGGCTTTCCCAACGTCATCACCGGCGATCTCGGCGGCACCTCGTTCGACGTGTCGC
TGGTGGTCGACGGCGCGACCTCGCTGGCGGCGCAGACCACGATCGATTTCGGCCTCGTCGTGCGCACGCCGATGATCGAG
ATCACCACCATCGGCGCCGGCGGCGGCTCGATCGCTCATGTCGATGCCGGCGGCCTGCTGCAGGTCGGCCCGGAGAGCGC
CGGCTCGCGTCCGGGTCCGGTGTGCTATGGCGGCGGCAACGACCGGCCGACGCTCACTGACGCCAACGTCGTGCTCGGCC
GCATCAATGCTGACAGGCCGATCGGCGGCAAGCTCAAGGCGCTCGATGTCGAGGCCGCCAAGCGCGCCATCGCCAAGCAT
GTCGGCGAGCCGCTGGGTCTCGGCGTGATGGAGGCGGCGGAAGCCATCGTGCGCATCGCCGACAGCAAGATGGCCGGGGC
CATCCGGCTGATGTCGATCGAGCGCGGCCATGATCCGGCGCGCTTCGCGGCGGTGCCGTTCGGCGGCGGCGGCGCGCTGC
ACGTCTCGGCGCTGATCAAGGATGTCGGGCTCAAGGCCGCGCTGGTGCCGCGCTATCCCGGCGTCACCTCGGCGCTCGGC
TGCGTCATCGCCGACATCCGCCATGACCAGGTGCAGACGCTCAACATGTTCGTCGACGGCCTCGACATCGCGGCGCTGAT
CCGGTGGATGCTGTCCGAGGCCGAGCATGCCCAGGGCGTGGTGAAGGACGCACGGCTCGCGGTCGAGCGTATCGACACGC
TGTTCGAGCTCGACATGCACTATGTCGGCCAGACCCACACCATCCGCGCGACCGTGCCGGCCGAGATCCGCGACGGTGCC
ATTGCGCTGACCGTGGAGGACGTGCGCAAGGCGTTCGAGACCGCCTATCAGAAGAGCTTCAGCCGGCTGTTGCCGGGCGT
GCCGATCAAGATCGTGAATCTGCGTACGGCGGCGATCGGCGTGCGGCCGCGCTTTGACCTCTCGGCCCTCGCGCCCGATG
CCGGCGCCGATTTGATGACCGCGCGGCTCGGCAGCCGCGACGTCTGGTTCGACGGCAAATGGTGGAGCACGGCGATCTAC
GCCCGGCTCGGTCTTCCGGTCGGCAGCGACATCGCCGGGCCCGCGATCCTGGAGCAGCCGGACGCGACCACGGTGATCGC
ACCCGGCTTCAAGGCGCGGGTCGACCAGTTCGGCAACGTCATCGTGGAGCGCAGCGCATGA

Upstream 100 bases:

>100_bases
TCTCGGCGGCGCAGGCGCGTGACGTCTACAAGGTTGCCGTGAAGGCAGACGGCGCGGTCGATGAAGCCGCGACGCACACG
CTCCGTGCGGGAGGTGCGGC

Downstream 100 bases:

>100_bases
GTGATCACAAGGTGTCGGCCGCGCAGAGCGCGCTGCTGATCGTCGATCTCCAGAACGATTTCCTCGATCCGAATGGCGCG
TATGGCCGTGCGGGACAGAC

Product: N-methylhydantoinase A

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MSKLGTIVGVDVGGTFTDLFYFDEAKRSFRTNKVPSNRGDEAVGFLEGLQGFGAVSHLTSIVHGTTVGTNALLERKGARI
GLITTRGFRDVLEMRRRDRRNTWGLWGDFVPVVERDMRAEVDERVLSDGTIRTAVDVAQVETTARELLAKGAQALAIVFV
NAFANPDNERRALEAVQAIWPNDNVACSSQILPEIREFERTSTTALNAYLQPVVGSYLQKLQNALAGEAFDGRFHIVQSN
GGVMSTDAARRFPIRTALSGPAAGVIAAAEIAKAAGFPNVITGDLGGTSFDVSLVVDGATSLAAQTTIDFGLVVRTPMIE
ITTIGAGGGSIAHVDAGGLLQVGPESAGSRPGPVCYGGGNDRPTLTDANVVLGRINADRPIGGKLKALDVEAAKRAIAKH
VGEPLGLGVMEAAEAIVRIADSKMAGAIRLMSIERGHDPARFAAVPFGGGGALHVSALIKDVGLKAALVPRYPGVTSALG
CVIADIRHDQVQTLNMFVDGLDIAALIRWMLSEAEHAQGVVKDARLAVERIDTLFELDMHYVGQTHTIRATVPAEIRDGA
IALTVEDVRKAFETAYQKSFSRLLPGVPIKIVNLRTAAIGVRPRFDLSALAPDAGADLMTARLGSRDVWFDGKWWSTAIY
ARLGLPVGSDIAGPAILEQPDATTVIAPGFKARVDQFGNVIVERSA

Sequences:

>Translated_686_residues
MSKLGTIVGVDVGGTFTDLFYFDEAKRSFRTNKVPSNRGDEAVGFLEGLQGFGAVSHLTSIVHGTTVGTNALLERKGARI
GLITTRGFRDVLEMRRRDRRNTWGLWGDFVPVVERDMRAEVDERVLSDGTIRTAVDVAQVETTARELLAKGAQALAIVFV
NAFANPDNERRALEAVQAIWPNDNVACSSQILPEIREFERTSTTALNAYLQPVVGSYLQKLQNALAGEAFDGRFHIVQSN
GGVMSTDAARRFPIRTALSGPAAGVIAAAEIAKAAGFPNVITGDLGGTSFDVSLVVDGATSLAAQTTIDFGLVVRTPMIE
ITTIGAGGGSIAHVDAGGLLQVGPESAGSRPGPVCYGGGNDRPTLTDANVVLGRINADRPIGGKLKALDVEAAKRAIAKH
VGEPLGLGVMEAAEAIVRIADSKMAGAIRLMSIERGHDPARFAAVPFGGGGALHVSALIKDVGLKAALVPRYPGVTSALG
CVIADIRHDQVQTLNMFVDGLDIAALIRWMLSEAEHAQGVVKDARLAVERIDTLFELDMHYVGQTHTIRATVPAEIRDGA
IALTVEDVRKAFETAYQKSFSRLLPGVPIKIVNLRTAAIGVRPRFDLSALAPDAGADLMTARLGSRDVWFDGKWWSTAIY
ARLGLPVGSDIAGPAILEQPDATTVIAPGFKARVDQFGNVIVERSA
>Mature_685_residues
SKLGTIVGVDVGGTFTDLFYFDEAKRSFRTNKVPSNRGDEAVGFLEGLQGFGAVSHLTSIVHGTTVGTNALLERKGARIG
LITTRGFRDVLEMRRRDRRNTWGLWGDFVPVVERDMRAEVDERVLSDGTIRTAVDVAQVETTARELLAKGAQALAIVFVN
AFANPDNERRALEAVQAIWPNDNVACSSQILPEIREFERTSTTALNAYLQPVVGSYLQKLQNALAGEAFDGRFHIVQSNG
GVMSTDAARRFPIRTALSGPAAGVIAAAEIAKAAGFPNVITGDLGGTSFDVSLVVDGATSLAAQTTIDFGLVVRTPMIEI
TTIGAGGGSIAHVDAGGLLQVGPESAGSRPGPVCYGGGNDRPTLTDANVVLGRINADRPIGGKLKALDVEAAKRAIAKHV
GEPLGLGVMEAAEAIVRIADSKMAGAIRLMSIERGHDPARFAAVPFGGGGALHVSALIKDVGLKAALVPRYPGVTSALGC
VIADIRHDQVQTLNMFVDGLDIAALIRWMLSEAEHAQGVVKDARLAVERIDTLFELDMHYVGQTHTIRATVPAEIRDGAI
ALTVEDVRKAFETAYQKSFSRLLPGVPIKIVNLRTAAIGVRPRFDLSALAPDAGADLMTARLGSRDVWFDGKWWSTAIYA
RLGLPVGSDIAGPAILEQPDATTVIAPGFKARVDQFGNVIVERSA

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=729, Percent_Identity=29.7668038408779, Blast_Score=221, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI133901900, Length=721, Percent_Identity=27.877947295423, Blast_Score=197, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322634, Length=671, Percent_Identity=28.4649776453055, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI45550492, Length=745, Percent_Identity=28.4563758389262, Blast_Score=203, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 72790; Mature: 72659

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLGTIVGVDVGGTFTDLFYFDEAKRSFRTNKVPSNRGDEAVGFLEGLQGFGAVSHLTS
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHH
IVHGTTVGTNALLERKGARIGLITTRGFRDVLEMRRRDRRNTWGLWGDFVPVVERDMRAE
HHHCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VDERVLSDGTIRTAVDVAQVETTARELLAKGAQALAIVFVNAFANPDNERRALEAVQAIW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCHHHHHHHHHHHHC
PNDNVACSSQILPEIREFERTSTTALNAYLQPVVGSYLQKLQNALAGEAFDGRFHIVQSN
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GGVMSTDAARRFPIRTALSGPAAGVIAAAEIAKAAGFPNVITGDLGGTSFDVSLVVDGAT
CCEEECCCHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCH
SLAAQTTIDFGLVVRTPMIEITTIGAGGGSIAHVDAGGLLQVGPESAGSRPGPVCYGGGN
HHHHHHHHCEEEEEECCEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCEEECCCC
DRPTLTDANVVLGRINADRPIGGKLKALDVEAAKRAIAKHVGEPLGLGVMEAAEAIVRIA
CCCCEEECCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DSKMAGAIRLMSIERGHDPARFAAVPFGGGGALHVSALIKDVGLKAALVPRYPGVTSALG
CCHHCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHH
CVIADIRHDQVQTLNMFVDGLDIAALIRWMLSEAEHAQGVVKDARLAVERIDTLFELDMH
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVGQTHTIRATVPAEIRDGAIALTVEDVRKAFETAYQKSFSRLLPGVPIKIVNLRTAAIG
HCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEE
VRPRFDLSALAPDAGADLMTARLGSRDVWFDGKWWSTAIYARLGLPVGSDIAGPAILEQP
CCCCCCHHHCCCCCCCHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
DATTVIAPGFKARVDQFGNVIVERSA
CCCEEECCCHHHHHHHHCCEEEECCC
>Mature Secondary Structure 
SKLGTIVGVDVGGTFTDLFYFDEAKRSFRTNKVPSNRGDEAVGFLEGLQGFGAVSHLTS
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHHH
IVHGTTVGTNALLERKGARIGLITTRGFRDVLEMRRRDRRNTWGLWGDFVPVVERDMRAE
HHHCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
VDERVLSDGTIRTAVDVAQVETTARELLAKGAQALAIVFVNAFANPDNERRALEAVQAIW
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCHHHHHHHHHHHHC
PNDNVACSSQILPEIREFERTSTTALNAYLQPVVGSYLQKLQNALAGEAFDGRFHIVQSN
CCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GGVMSTDAARRFPIRTALSGPAAGVIAAAEIAKAAGFPNVITGDLGGTSFDVSLVVDGAT
CCEEECCCHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCH
SLAAQTTIDFGLVVRTPMIEITTIGAGGGSIAHVDAGGLLQVGPESAGSRPGPVCYGGGN
HHHHHHHHCEEEEEECCEEEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCCEEECCCC
DRPTLTDANVVLGRINADRPIGGKLKALDVEAAKRAIAKHVGEPLGLGVMEAAEAIVRIA
CCCCEEECCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
DSKMAGAIRLMSIERGHDPARFAAVPFGGGGALHVSALIKDVGLKAALVPRYPGVTSALG
CCHHCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHH
CVIADIRHDQVQTLNMFVDGLDIAALIRWMLSEAEHAQGVVKDARLAVERIDTLFELDMH
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVGQTHTIRATVPAEIRDGAIALTVEDVRKAFETAYQKSFSRLLPGVPIKIVNLRTAAIG
HCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEE
VRPRFDLSALAPDAGADLMTARLGSRDVWFDGKWWSTAIYARLGLPVGSDIAGPAILEQP
CCCCCCHHHCCCCCCCHHHHHHCCCCCEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCC
DATTVIAPGFKARVDQFGNVIVERSA
CCCEEECCCHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]