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The map label for this gene is rutB [H]

Identifier: 146342349

GI number: 146342349

Start: 5686409

End: 5687023

Strand: Reverse

Name: rutB [H]

Synonym: BRADO5505

Alternate gene names: 146342349

Gene position: 5687023-5686409 (Counterclockwise)

Preceding gene: 146342350

Following gene: 146342348

Centisome position: 76.27

GC content: 64.72

Gene sequence:

>615_bases
ATGAGTGATCACAAGGTGTCGGCCGCGCAGAGCGCGCTGCTGATCGTCGATCTCCAGAACGATTTCCTCGATCCGAATGG
CGCGTATGGCCGTGCGGGACAGACGGCGGCTGACATCGCGGCGCTGCCGCCGCGGCTCAAGCCGTTTGCCGATCTGATGC
GAAGCAAAGGCGGCTTCGTCATCTCCACCCAGTTCACGCTGGTGCCGGGCAAGGGCGGTGACCCCCTGATCTCGCCGCAT
CTGAAGCAGCTGCGGCCGTTCCTCGCCAAGGGCGATTTCCTGCCCGGCGCGTGGGGCCATCAGCTGGTGGAGACGTTGCA
GCCGGCCGACGTCACGATCGAGAAGATCGCCTATTCGGCATTCTACATGACACGGCTCGAATGGGTGCTGCGCAAGTGCC
GCATCGAGAAGCTCTATGTCGCGGGCATCGTCACCAATGGCGGCGTGGCGTCGACCGTGCGTGACGCGCACATTCGTGAC
TTCGAGACGGTCGTGCTCGAGGATGGATGCGCCGCGTTCACGGCGGAGACGCATGCGACCGCGATCGCCGCGCTGAAGCC
GGTGTGCCGCGTCACCTCGATCGCGGAGGCGATCAAGGAATTGGCCGCGTCATGA

Upstream 100 bases:

>100_bases
GGCCCGCGATCCTGGAGCAGCCGGACGCGACCACGGTGATCGCACCCGGCTTCAAGGCGCGGGTCGACCAGTTCGGCAAC
GTCATCGTGGAGCGCAGCGC

Downstream 100 bases:

>100_bases
GTTCGGTCGAGACGATCGATCGCACGCCCGAGCTTGCCGTCGACTGCCTGATCATCGGCGCGGGTGCGGCTGGGCTCGTC
GCTGCGCTGTCGGCGGCCGA

Product: putative isochorismatase family protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSDHKVSAAQSALLIVDLQNDFLDPNGAYGRAGQTAADIAALPPRLKPFADLMRSKGGFVISTQFTLVPGKGGDPLISPH
LKQLRPFLAKGDFLPGAWGHQLVETLQPADVTIEKIAYSAFYMTRLEWVLRKCRIEKLYVAGIVTNGGVASTVRDAHIRD
FETVVLEDGCAAFTAETHATAIAALKPVCRVTSIAEAIKELAAS

Sequences:

>Translated_204_residues
MSDHKVSAAQSALLIVDLQNDFLDPNGAYGRAGQTAADIAALPPRLKPFADLMRSKGGFVISTQFTLVPGKGGDPLISPH
LKQLRPFLAKGDFLPGAWGHQLVETLQPADVTIEKIAYSAFYMTRLEWVLRKCRIEKLYVAGIVTNGGVASTVRDAHIRD
FETVVLEDGCAAFTAETHATAIAALKPVCRVTSIAEAIKELAAS
>Mature_203_residues
SDHKVSAAQSALLIVDLQNDFLDPNGAYGRAGQTAADIAALPPRLKPFADLMRSKGGFVISTQFTLVPGKGGDPLISPHL
KQLRPFLAKGDFLPGAWGHQLVETLQPADVTIEKIAYSAFYMTRLEWVLRKCRIEKLYVAGIVTNGGVASTVRDAHIRDF
ETVVLEDGCAAFTAETHATAIAALKPVCRVTSIAEAIKELAAS

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=206, Percent_Identity=31.0679611650485, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081992, Length=199, Percent_Identity=31.1557788944724, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 21902; Mature: 21771

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHKVSAAQSALLIVDLQNDFLDPNGAYGRAGQTAADIAALPPRLKPFADLMRSKGGFV
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCCEE
ISTQFTLVPGKGGDPLISPHLKQLRPFLAKGDFLPGAWGHQLVETLQPADVTIEKIAYSA
EEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
FYMTRLEWVLRKCRIEKLYVAGIVTNGGVASTVRDAHIRDFETVVLEDGCAAFTAETHAT
HHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHCHHHEEEEECCCCEEEHHHHHH
AIAALKPVCRVTSIAEAIKELAAS
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDHKVSAAQSALLIVDLQNDFLDPNGAYGRAGQTAADIAALPPRLKPFADLMRSKGGFV
CCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHCCCCEE
ISTQFTLVPGKGGDPLISPHLKQLRPFLAKGDFLPGAWGHQLVETLQPADVTIEKIAYSA
EEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHH
FYMTRLEWVLRKCRIEKLYVAGIVTNGGVASTVRDAHIRDFETVVLEDGCAAFTAETHAT
HHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHCHHHEEEEECCCCEEEHHHHHH
AIAALKPVCRVTSIAEAIKELAAS
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA