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The map label for this gene is nifD [H]

Identifier: 146342285

GI number: 146342285

Start: 5626138

End: 5627640

Strand: Reverse

Name: nifD [H]

Synonym: BRADO5439

Alternate gene names: 146342285

Gene position: 5627640-5626138 (Counterclockwise)

Preceding gene: 146342286

Following gene: 146342284

Centisome position: 75.47

GC content: 62.54

Gene sequence:

>1503_bases
ATGAGCGTTATCGAGAAACAAAGCGTTGCCGAGATCAAGGCACGCAACAAGGAACTGATCGCCGAGGTCCTCAAGGTCTA
CCCCGAGAAGACCGCCAAGCGTCGCGCCAAGCATCTCAACGTCCACGAGTCCGGCAAGTCGGACTGCGGCGTCAAGTCGA
ACATCAAGTCGATCCCGGGCGTGATGACCATCCGCGGCTGCGCCTACGCCGGCTCGAAGGGCGTGGTGTGGGGTCCGATC
AAGGACATGATCCACATCAGCCACGGCCCGGTCGGCTGCGGCCAGTATTCCTGGGCGGCGCGCCGCAACTACTACATCGG
CACGACCGGCATCGACACCTTCGTCACGATGCAGTTCACCTCCGACTTCCAGGAGAAGGACATCGTGTTCGGCGGCGACA
AGAAGCTCGCCAAGATCATGGACGAGATCCAGGAGCTGTTCCCGCTGAACAACGGCATCACCGTGCAGTCGGAATGCCCG
ATCGGCCTGATCGGCGACGACATCGAGGCGGTGTCGAAGGTCAAGTCGAAGGAGTACGACGGCAAGACCATCGTCCCCGT
CCGCTGCGAAGGCTTCCGCGGTGTCTCCCAGTCGCTCGGCCACCACATCGCCAACGACGCGATCCGGGACTGGGTGTTCG
ACAAGCTGCCGGCCGACGCCGCGCCGCGCTTCGAGCCGTCGGATTACGACGTCGCGATCATCGGCGACTACAACATCGGC
GGCGACGCCTGGTCCTCGCGCATCCTGCTCGAGGAGATGGGCCTGCGCGTGATCGCCCAGTGGTCGGGCGACGGCTCACT
GGCCGAGCTCGAGGCGACGCCGAAAGCGAAGCTCAACGTGCTGCACTGCTACCGCTCGATGAACTACATCTCGCGTCACA
TGGAAGAGAAGTTCGGTATTCCGTGGTGCGAGTACAACTTCTTCGGGCCGAGCAAGATCGCCGAATCGCTGCGTAAGATC
GCCAGCTATTTCGACGACAAGATCAAGGAAGGTGCCGAGCGCGTCATCGCCAAGTACCAGCCCCTGATGGACGCCGTGAT
CGCCAAGTACCGGCCGCGCCTGGAAGGCAAGACCGTGATGCTGTTCGTCGGCGGCCTGCGTCCGCGCCACGTGATCGGCG
CCTACGAGGACCTCGGCATGGAGGTCGTCGGCACCGGTTACGAGTTCGCCCACAACGACGACTATCAGCGCACCGCCCAG
CATTACGTCAAGGACGGCACGCTGATCTATGACGACGTGACCGGCTACGAGTTCGAGAAGTTCGTCGAGAAGGTCCAGCC
GGACCTGGTCGGCTCGGGCATCAAGGAAAAGTACGTCTTCCAGAAGATGGGCGTGCCGTTCCGCCAGATGCACTCCTGGG
ACTACTCGGGTCCCTACCATGGCTATGACGGGTTCGCGATCTTCGCCCGCGACATGGACATGGCGATCAACTCGCCGGTC
TGGAAGATGACCAAGGCTCCCTGGAAGCAGGCGCCGAAGCCCCTTCTCCAGGCGGCCGAGTAA

Upstream 100 bases:

>100_bases
CCGACCTCGCCGCCACGGCCTAAAGCGTCACCGGTCATCCGGCCCGTCGTGTTCGACGGGCCGGGCGATCTCCGCAACCG
ATCATCGAGGAAAAAGCCAG

Downstream 100 bases:

>100_bases
CAACCACCACCCACGGCCTGGCTCTCCTTCGGCGGAGAGCCAGCGCTCCCTGATCGACACAGGAACATAGAGAGGGTTTT
CCCATGACACAGTCTGCCGA

Product: nitrogenase molybdenum-iron protein subunit alpha, nifD

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSVIEKQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPI
KDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIQELFPLNNGITVQSECP
IGLIGDDIEAVSKVKSKEYDGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKLPADAAPRFEPSDYDVAIIGDYNIG
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGIPWCEYNFFGPSKIAESLRKI
ASYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVMLFVGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYQRTAQ
HYVKDGTLIYDDVTGYEFEKFVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
WKMTKAPWKQAPKPLLQAAE

Sequences:

>Translated_500_residues
MSVIEKQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPI
KDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIQELFPLNNGITVQSECP
IGLIGDDIEAVSKVKSKEYDGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKLPADAAPRFEPSDYDVAIIGDYNIG
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGIPWCEYNFFGPSKIAESLRKI
ASYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVMLFVGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYQRTAQ
HYVKDGTLIYDDVTGYEFEKFVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
WKMTKAPWKQAPKPLLQAAE
>Mature_499_residues
SVIEKQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPGVMTIRGCAYAGSKGVVWGPIK
DMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFTSDFQEKDIVFGGDKKLAKIMDEIQELFPLNNGITVQSECPI
GLIGDDIEAVSKVKSKEYDGKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKLPADAAPRFEPSDYDVAIIGDYNIGG
DAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGIPWCEYNFFGPSKIAESLRKIA
SYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVMLFVGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYQRTAQH
YVKDGTLIYDDVTGYEFEKFVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVW
KMTKAPWKQAPKPLLQAAE

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56153; Mature: 56022

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIEKQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPG
CCCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFT
EEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEEEEEEC
SDFQEKDIVFGGDKKLAKIMDEIQELFPLNNGITVQSECPIGLIGDDIEAVSKVKSKEYD
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCC
GKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKLPADAAPRFEPSDYDVAIIGDYNIG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGI
CHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PWCEYNFFGPSKIAESLRKIASYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVM
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LFVGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYQRTAQHYVKDGTLIYDDVTGYEFEK
EEECCCCCHHHHCCHHHCCCEEEECCEECCCCCCHHHHHHHHHCCCCEEEECCCCCHHHH
FVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
HHHHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHHHCCCH
WKMTKAPWKQAPKPLLQAAE
HHHHCCCHHHCCHHHHHCCC
>Mature Secondary Structure 
SVIEKQSVAEIKARNKELIAEVLKVYPEKTAKRRAKHLNVHESGKSDCGVKSNIKSIPG
CCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCC
VMTIRGCAYAGSKGVVWGPIKDMIHISHGPVGCGQYSWAARRNYYIGTTGIDTFVTMQFT
EEEEEEEEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEECCCCCEEEEEEEC
SDFQEKDIVFGGDKKLAKIMDEIQELFPLNNGITVQSECPIGLIGDDIEAVSKVKSKEYD
CCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCHHHHHHHHHCCCC
GKTIVPVRCEGFRGVSQSLGHHIANDAIRDWVFDKLPADAAPRFEPSDYDVAIIGDYNIG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
GDAWSSRILLEEMGLRVIAQWSGDGSLAELEATPKAKLNVLHCYRSMNYISRHMEEKFGI
CHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
PWCEYNFFGPSKIAESLRKIASYFDDKIKEGAERVIAKYQPLMDAVIAKYRPRLEGKTVM
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LFVGGLRPRHVIGAYEDLGMEVVGTGYEFAHNDDYQRTAQHYVKDGTLIYDDVTGYEFEK
EEECCCCCHHHHCCHHHCCCEEEECCEECCCCCCHHHHHHHHHCCCCEEEECCCCCHHHH
FVEKVQPDLVGSGIKEKYVFQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPV
HHHHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHHHCCCH
WKMTKAPWKQAPKPLLQAAE
HHHHCCCHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275; 6588133 [H]