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The map label for this gene is phnM [H]

Identifier: 146342216

GI number: 146342216

Start: 5574388

End: 5575575

Strand: Direct

Name: phnM [H]

Synonym: BRADO5368

Alternate gene names: 146342216

Gene position: 5574388-5575575 (Clockwise)

Preceding gene: 146342215

Following gene: 146342217

Centisome position: 74.76

GC content: 68.6

Gene sequence:

>1188_bases
ATGACAGACATCTCCATCTCCTCGGGCCGCTGTCTGATCGGCGCCGACTTCGCTGACACCTCTCTGACCGTATCCGAAGG
CGTGATCGACCGGCTCGATGTCCCCACGCGCGGTGCCGCGCTGGATCTCGATGCCGCCGGCCTCACGGTCCTGCCCGGGA
TCGTCGACATCCATGGCGATGCGTTCGAGCGGCAGATGATGCCGCGGGCTGGCGTCGACTTCCCGATCGACGTCGCGCTT
GTCGACAGCGACCGCCAGGCCGTCAGCAACGGCATCACCACGGTGTTCCACGCGACCACATGGTCGTGGGAGCCCGGCCT
GCGCAGTGCCGGCAATGCACGCCAGTTGTTGGAGGCGATCGAGACCCTGCGTCCGCGGCTCGCCGCGGACACGCGCTTCC
ACCTCCGGCACGAGACCTACAATCTCGACGACGAGGATGAGATCCGCCAGTTGCTCGCTGCACGGCGGATCGACCTGTTT
GCCTTCAACGATCATATGGACTCGACTGTCGCGAGCCTGGAGAAGCCGCACAAGCGCCAGCGCATGGTCGATCGCACAGG
CTTGTCCGGCGACGAGTTCGACCGCCTCGTCGGCCGCATCGCGGCTCGCGCGGACGCGGTGCCGTCCTCCATCTCACGTC
TGGCGGAGACGGCGCGCACGGCCGGGGTGCGCATGCTGTCGCACGACGACGAAAGTCCGGAGATGCGCCGCGCCTTTCGC
GGTCAGGGCGTCTCCATCGCCGAGTTCCCGGTCAACGAGGAAACGGCGCGCGAGGCCGCCGCAGGCGGCGACGCCATCGT
GTTCGGTGCGCCGAACGTGGTGCGCGGCGGCAGCCATACCGGCTGGACCAAGGCAAGCGACATGATCGCCAAGGGCCTCT
GCTCGGTGCTGGCATCCGACTACTACTATCCTGCGCCCCTGCTCGCGGCCTATCGTCTCGTCGCGGACGGCGTTCTGCCG
CTGGCCCGCGCCTGGAGCCTGATTTCAGCCGGCCCCGCGCAGGCCGCAGGCCTGTCCGACCGCGGCACGCTCGCACCAGG
GCAGCGGGCGGACATCGTGCTGGTCGATGACAGCTCCTCGATGCGTCCACGCATCGTCGCCGTGATCGCGGCCGGGCGTC
TCGTCCATCTCACGGACGCTGACCGTCTCACCACGCATGCGGTACGCCGCGCAGCGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
CGGCGCTACCGGATTTCCGGCAGCTCGAGATCGGCCAATTCCTGGACAAATACGAGACGGCTCTCGACGGCAGGCGCCAG
GTGCGGCAGAGTGCCGCGCA

Downstream 100 bases:

>100_bases
GCACCGTGGAAGGCTATCCGCGCTACGCCATCTACTACACGCCTGCGCCGGACACAGCGTTGAGCGTGTTTGGCGCTCAT
CTGCTCGGCTACGACGCCTG

Product: putative metal-dependent hydrolase involved in phosphonate metabolism/PhnM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTDISISSGRCLIGADFADTSLTVSEGVIDRLDVPTRGAALDLDAAGLTVLPGIVDIHGDAFERQMMPRAGVDFPIDVAL
VDSDRQAVSNGITTVFHATTWSWEPGLRSAGNARQLLEAIETLRPRLAADTRFHLRHETYNLDDEDEIRQLLAARRIDLF
AFNDHMDSTVASLEKPHKRQRMVDRTGLSGDEFDRLVGRIAARADAVPSSISRLAETARTAGVRMLSHDDESPEMRRAFR
GQGVSIAEFPVNEETAREAAAGGDAIVFGAPNVVRGGSHTGWTKASDMIAKGLCSVLASDYYYPAPLLAAYRLVADGVLP
LARAWSLISAGPAQAAGLSDRGTLAPGQRADIVLVDDSSSMRPRIVAVIAAGRLVHLTDADRLTTHAVRRAAAAE

Sequences:

>Translated_395_residues
MTDISISSGRCLIGADFADTSLTVSEGVIDRLDVPTRGAALDLDAAGLTVLPGIVDIHGDAFERQMMPRAGVDFPIDVAL
VDSDRQAVSNGITTVFHATTWSWEPGLRSAGNARQLLEAIETLRPRLAADTRFHLRHETYNLDDEDEIRQLLAARRIDLF
AFNDHMDSTVASLEKPHKRQRMVDRTGLSGDEFDRLVGRIAARADAVPSSISRLAETARTAGVRMLSHDDESPEMRRAFR
GQGVSIAEFPVNEETAREAAAGGDAIVFGAPNVVRGGSHTGWTKASDMIAKGLCSVLASDYYYPAPLLAAYRLVADGVLP
LARAWSLISAGPAQAAGLSDRGTLAPGQRADIVLVDDSSSMRPRIVAVIAAGRLVHLTDADRLTTHAVRRAAAAE
>Mature_394_residues
TDISISSGRCLIGADFADTSLTVSEGVIDRLDVPTRGAALDLDAAGLTVLPGIVDIHGDAFERQMMPRAGVDFPIDVALV
DSDRQAVSNGITTVFHATTWSWEPGLRSAGNARQLLEAIETLRPRLAADTRFHLRHETYNLDDEDEIRQLLAARRIDLFA
FNDHMDSTVASLEKPHKRQRMVDRTGLSGDEFDRLVGRIAARADAVPSSISRLAETARTAGVRMLSHDDESPEMRRAFRG
QGVSIAEFPVNEETAREAAAGGDAIVFGAPNVVRGGSHTGWTKASDMIAKGLCSVLASDYYYPAPLLAAYRLVADGVLPL
ARAWSLISAGPAQAAGLSDRGTLAPGQRADIVLVDDSSSMRPRIVAVIAAGRLVHLTDADRLTTHAVRRAAAAE

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3454

COG function: function code P; Metal-dependent hydrolase involved in phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790533, Length=361, Percent_Identity=30.7479224376731, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059
- InterPro:   IPR012696 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: NA

Molecular weight: Translated: 42382; Mature: 42251

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDISISSGRCLIGADFADTSLTVSEGVIDRLDVPTRGAALDLDAAGLTVLPGIVDIHGD
CCCCCCCCCCEEEECCCCCCCEEHHHCCHHHCCCCCCCCEEECCCCCCCCCCCCEECCCC
AFERQMMPRAGVDFPIDVALVDSDRQAVSNGITTVFHATTWSWEPGLRSAGNARQLLEAI
HHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHH
ETLRPRLAADTRFHLRHETYNLDDEDEIRQLLAARRIDLFAFNDHMDSTVASLEKPHKRQ
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHCHHHHH
RMVDRTGLSGDEFDRLVGRIAARADAVPSSISRLAETARTAGVRMLSHDDESPEMRRAFR
HHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHC
GQGVSIAEFPVNEETAREAAAGGDAIVFGAPNVVRGGSHTGWTKASDMIAKGLCSVLASD
CCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
YYYPAPLLAAYRLVADGVLPLARAWSLISAGPAQAAGLSDRGTLAPGQRADIVLVDDSSS
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCEEEEECCCC
MRPRIVAVIAAGRLVHLTDADRLTTHAVRRAAAAE
CCCEEEEEEECCCEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDISISSGRCLIGADFADTSLTVSEGVIDRLDVPTRGAALDLDAAGLTVLPGIVDIHGD
CCCCCCCCCEEEECCCCCCCEEHHHCCHHHCCCCCCCCEEECCCCCCCCCCCCEECCCC
AFERQMMPRAGVDFPIDVALVDSDRQAVSNGITTVFHATTWSWEPGLRSAGNARQLLEAI
HHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHH
ETLRPRLAADTRFHLRHETYNLDDEDEIRQLLAARRIDLFAFNDHMDSTVASLEKPHKRQ
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHCHHHHH
RMVDRTGLSGDEFDRLVGRIAARADAVPSSISRLAETARTAGVRMLSHDDESPEMRRAFR
HHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHC
GQGVSIAEFPVNEETAREAAAGGDAIVFGAPNVVRGGSHTGWTKASDMIAKGLCSVLASD
CCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
YYYPAPLLAAYRLVADGVLPLARAWSLISAGPAQAAGLSDRGTLAPGQRADIVLVDDSSS
CCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCEEEEECCCC
MRPRIVAVIAAGRLVHLTDADRLTTHAVRRAAAAE
CCCEEEEEEECCCEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]