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The map label for this gene is cobQ

Identifier: 146341794

GI number: 146341794

Start: 5112986

End: 5114473

Strand: Direct

Name: cobQ

Synonym: BRADO4913

Alternate gene names: 146341794

Gene position: 5112986-5114473 (Clockwise)

Preceding gene: 146341793

Following gene: 146341796

Centisome position: 68.57

GC content: 68.21

Gene sequence:

>1488_bases
ATGGCTGCGCGCGCGATCATGTTCCAGGGCACCGGTTCCGACGTCGGCAAATCGCTGATGGTCGCCGGACTCGCGCGCGC
GCTGACTCTGCGCGGGCTGACGGTCGCGCCGTTCAAGCCGCAGAACATGTCGAACAACGCCGCCGTCACCGCGGACGGCG
GCGAGATCGGCCGCGCCCAGGCGCTGCAGGCGCGGGCGGCGCGCCGGCCGATGACGGTGCACATGAACCCGGTGCTGTTG
AAGCCGCAGAGCGAGGTCGGCTCGCAGGTCGTGGTGCAGGGACGCGTGATCGGCAACGCCAAGGCGTCGGCCTATCAGGC
GATGAAGCCGCAGCTGATGGCGGCCGTGTTGGAGAGCTTCCATCGCCTCGCCGCCGACGCAGACATCGTGCTGGTCGAAG
GCGCGGGCTCGGCATCGGAGATCAACCTTCGCGCGGGCGACATCGCCAACATGGGCTTCGCGCAGGCGACGCAGACTCCG
GTCGTGATCATCGGCGACATCGATCGCGGCGGTGTCATTGCGAGCCTCGTCGGCACCAAGGCGGTTCTCGCGCCGGACGA
TGCCGCCCTCGTCGCAGGCTTTTTGGTCAACCGATTTCGCGGCGATCCGGCGCTGTTCGCAGCGGGCATGAACGAGATCG
CGGCACGGACCGGCTGGGCATCGCTCGGGCTCATTCCGCATTTTTCGGATGCGCGCCGGCTGCCGGCGGAGGATGCACTC
GGCCTGCCCGGACATGGCGCTGCAGGCGGACAACGCCCGAAGGTCGTCGTGCTCGCCTATCCCCGCATCTCCAATTTCGA
CGAGTTCGACCCGTTGCGGCTCGAAGATGGGATCGATCTGCAGTTTCTGCGGCCGGGTACGCCGATCCCAGGCGATGCGG
CCGTGGCGATCCTGCCGGGGTCGAAGGCCACCATCGCCGATCTCCAGGCCTTGCGCGAGACCGGCTGGGACATCGATCTC
TCGGCACATCTGCGCCGCGGCGGCCGCGTGCTCGGCATCTGCGGTGGCTACCAGATGCTCGGACGAAAAATCAGCGATCC
CGACGGCCATGAAGGCGCACCCGGTTCCGTCGCGGGGCTTGGCCTGCTCGACGTCGAGACGACCCTCACCGACGACAAGG
CGTTGCGCGAGGTTCACGGCGCGCTCACCGAGGATCAGATCCCGTTCCGCGGCTACGAGATGCATATCGGCCGCACCGAC
GGGCCGACCGCTCGAGCTCCGTTTCTCACCTTCGCCGATGGCCGAAGCGATGGCGCGGTCGCGCGCGAGGGGCAGATCGC
CGGTTGCTATGTGCACGGCCTGTTCGCCGATGACGGCCTTCGCGCGCATTGGCTCGGTCGTCTCGGGGCCGTCACGACCG
GGCTGGCCTACGAGAGCGATGTCGAGGCGACCCTGGATGGGCTTGCGGCGCATCTCGAGCGCCACGTCAACGTCAGCGGA
ATTCTTGCGCTGGCGCGTAGGCCGACGCCGCGTCCTACAGCACCTTGA

Upstream 100 bases:

>100_bases
CGAGCTGATCGAGGCCGCCGATCTCGTCACTGAGATGACCCTGATCAAGCATCACTTCGCAGCTGGCGTGAAGGCGCAGG
AAGGCATCGAGTTCTAGTCC

Downstream 100 bases:

>100_bases
CGAGGAAGATGATCGCAACGAACAGCGCCTGGATCACACAAGCGAACCGGTAGAGCTGGAGGGCGCGACGGATATCGGAG
GCGTTCAGATCGGCGCGTCC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MAARAIMFQGTGSDVGKSLMVAGLARALTLRGLTVAPFKPQNMSNNAAVTADGGEIGRAQALQARAARRPMTVHMNPVLL
KPQSEVGSQVVVQGRVIGNAKASAYQAMKPQLMAAVLESFHRLAADADIVLVEGAGSASEINLRAGDIANMGFAQATQTP
VVIIGDIDRGGVIASLVGTKAVLAPDDAALVAGFLVNRFRGDPALFAAGMNEIAARTGWASLGLIPHFSDARRLPAEDAL
GLPGHGAAGGQRPKVVVLAYPRISNFDEFDPLRLEDGIDLQFLRPGTPIPGDAAVAILPGSKATIADLQALRETGWDIDL
SAHLRRGGRVLGICGGYQMLGRKISDPDGHEGAPGSVAGLGLLDVETTLTDDKALREVHGALTEDQIPFRGYEMHIGRTD
GPTARAPFLTFADGRSDGAVAREGQIAGCYVHGLFADDGLRAHWLGRLGAVTTGLAYESDVEATLDGLAAHLERHVNVSG
ILALARRPTPRPTAP

Sequences:

>Translated_495_residues
MAARAIMFQGTGSDVGKSLMVAGLARALTLRGLTVAPFKPQNMSNNAAVTADGGEIGRAQALQARAARRPMTVHMNPVLL
KPQSEVGSQVVVQGRVIGNAKASAYQAMKPQLMAAVLESFHRLAADADIVLVEGAGSASEINLRAGDIANMGFAQATQTP
VVIIGDIDRGGVIASLVGTKAVLAPDDAALVAGFLVNRFRGDPALFAAGMNEIAARTGWASLGLIPHFSDARRLPAEDAL
GLPGHGAAGGQRPKVVVLAYPRISNFDEFDPLRLEDGIDLQFLRPGTPIPGDAAVAILPGSKATIADLQALRETGWDIDL
SAHLRRGGRVLGICGGYQMLGRKISDPDGHEGAPGSVAGLGLLDVETTLTDDKALREVHGALTEDQIPFRGYEMHIGRTD
GPTARAPFLTFADGRSDGAVAREGQIAGCYVHGLFADDGLRAHWLGRLGAVTTGLAYESDVEATLDGLAAHLERHVNVSG
ILALARRPTPRPTAP
>Mature_494_residues
AARAIMFQGTGSDVGKSLMVAGLARALTLRGLTVAPFKPQNMSNNAAVTADGGEIGRAQALQARAARRPMTVHMNPVLLK
PQSEVGSQVVVQGRVIGNAKASAYQAMKPQLMAAVLESFHRLAADADIVLVEGAGSASEINLRAGDIANMGFAQATQTPV
VIIGDIDRGGVIASLVGTKAVLAPDDAALVAGFLVNRFRGDPALFAAGMNEIAARTGWASLGLIPHFSDARRLPAEDALG
LPGHGAAGGQRPKVVVLAYPRISNFDEFDPLRLEDGIDLQFLRPGTPIPGDAAVAILPGSKATIADLQALRETGWDIDLS
AHLRRGGRVLGICGGYQMLGRKISDPDGHEGAPGSVAGLGLLDVETTLTDDKALREVHGALTEDQIPFRGYEMHIGRTDG
PTARAPFLTFADGRSDGAVAREGQIAGCYVHGLFADDGLRAHWLGRLGAVTTGLAYESDVEATLDGLAAHLERHVNVSGI
LALARRPTPRPTAP

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_BRASO (A4YXJ9)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001206842.1
- ProteinModelPortal:   A4YXJ9
- SMR:   A4YXJ9
- STRING:   A4YXJ9
- GeneID:   5115944
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO4913
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   BSP376:BRADO4913-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 51647; Mature: 51515

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 334-334 ACT_SITE 432-432

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAIMFQGTGSDVGKSLMVAGLARALTLRGLTVAPFKPQNMSNNAAVTADGGEIGRAQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCHHH
ALQARAARRPMTVHMNPVLLKPQSEVGSQVVVQGRVIGNAKASAYQAMKPQLMAAVLESF
HHHHHHHCCCEEEECCCEEECCCHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRLAADADIVLVEGAGSASEINLRAGDIANMGFAQATQTPVVIIGDIDRGGVIASLVGTK
HHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCE
AVLAPDDAALVAGFLVNRFRGDPALFAAGMNEIAARTGWASLGLIPHFSDARRLPAEDAL
EEECCCHHHHHHHHHHHHHCCCHHEEECCHHHHHHHCCCHHCCCCCCCCHHHCCCCHHCC
GLPGHGAAGGQRPKVVVLAYPRISNFDEFDPLRLEDGIDLQFLRPGTPIPGDAAVAILPG
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECC
SKATIADLQALRETGWDIDLSAHLRRGGRVLGICGGYQMLGRKISDPDGHEGAPGSVAGL
CCCHHHHHHHHHHCCCCEEEHHHHCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEE
GLLDVETTLTDDKALREVHGALTEDQIPFRGYEMHIGRTDGPTARAPFLTFADGRSDGAV
EEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
AREGQIAGCYVHGLFADDGLRAHWLGRLGAVTTGLAYESDVEATLDGLAAHLERHVNVSG
ECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHH
ILALARRPTPRPTAP
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
AARAIMFQGTGSDVGKSLMVAGLARALTLRGLTVAPFKPQNMSNNAAVTADGGEIGRAQ
CCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCEEEEECCCCCCHHH
ALQARAARRPMTVHMNPVLLKPQSEVGSQVVVQGRVIGNAKASAYQAMKPQLMAAVLESF
HHHHHHHCCCEEEECCCEEECCCHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRLAADADIVLVEGAGSASEINLRAGDIANMGFAQATQTPVVIIGDIDRGGVIASLVGTK
HHHHCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCE
AVLAPDDAALVAGFLVNRFRGDPALFAAGMNEIAARTGWASLGLIPHFSDARRLPAEDAL
EEECCCHHHHHHHHHHHHHCCCHHEEECCHHHHHHHCCCHHCCCCCCCCHHHCCCCHHCC
GLPGHGAAGGQRPKVVVLAYPRISNFDEFDPLRLEDGIDLQFLRPGTPIPGDAAVAILPG
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCEEEEECC
SKATIADLQALRETGWDIDLSAHLRRGGRVLGICGGYQMLGRKISDPDGHEGAPGSVAGL
CCCHHHHHHHHHHCCCCEEEHHHHCCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEE
GLLDVETTLTDDKALREVHGALTEDQIPFRGYEMHIGRTDGPTARAPFLTFADGRSDGAV
EEEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCE
AREGQIAGCYVHGLFADDGLRAHWLGRLGAVTTGLAYESDVEATLDGLAAHLERHVNVSG
ECCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHH
ILALARRPTPRPTAP
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA