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The map label for this gene is dusA [H]

Identifier: 146341642

GI number: 146341642

Start: 4937643

End: 4938662

Strand: Reverse

Name: dusA [H]

Synonym: BRADO4745

Alternate gene names: 146341642

Gene position: 4938662-4937643 (Counterclockwise)

Preceding gene: 146341643

Following gene: 146341635

Centisome position: 66.23

GC content: 66.57

Gene sequence:

>1020_bases
ATGGCTGAAATATCAGACGAAATAGGTAAATCAGGAGCTTGGCGCTTCGCCGTGGCGCCGATGATGGATTGGACAGACCG
GCACTGCCGCGTGTTCCATCGGCTGCTGTCGCGCCGGGCTCTGCTTTACACCGAGATGGTCACGACGGGTGCCGTCATTC
ATGGCGACCGGACGCGGTTGCTCGGCTTCGATCGGAGCGAGCATCCGGTGGCGCTGCAGCTCGGCGGTTCTGATCCGGCC
GACCTCGCGACCTCGGCCCGGATCGGCGAGGATTTCGGTTATGACGAAATCAACCTCAATGTCGGCTGTCCGTCGGACCG
GGTGAAGGATGGCCGCTTCGGCGCCTGCCTGATGGCGGAGCCGGCGCTGGTCGCGGCCTGCGTCGCGGCGATGAAGCGCG
TGGTGACAATTCCGGTCACGGTGAAGTGCCGCATCGGCATCGATGATCAGGATCCGGAGGTCGCGCTCGATGCATTGGCG
GATGCCGTGGTCGATGCAGGCGCTGACGCGCTGATCGTGCATGCGCGCAAGGCCTGGCTGAACGGGCTGTCGCCGAAGGA
GAACCGCGACATCCCGCCGCTCGACTATGACAGGGTGTACCGGCTGAAGGCGCGGCTGCCGCGGGTGCCGGTCATCATCA
ATGGCGGCATCACCAGCATCGAAGCGGCGCAGGATCATCTCGCGCATGTCGATGGCGTGATGCTCGGGCGCGCGGCCTAT
CAGGAGCCGTGGCGATTGCTCGACGTCGATCCCGTGCTGTTCGGCGAGGCGGGGCCACAGCGGAGCATGAAGGACGCGCT
GGCTGCGCTCGAGCCCTATATCGAGCGCCAAGTCGCGCAGGGCATGCGGCTGCACGCGATCACGCGGCATCTCGTCGGAG
CGTATCATGCCGTGCCCGGCGCGCGCGCGTTCCGCCGCCATCTCGCCGAGCGCGGCGTCAAGCCTGACGCAGGCATCGAG
GTGTTGCGCGAGGCCGTGGCGATGATCGAGGAGCGCGCGCCTTCCGTCGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
GCGCTACAGCATTGTTGTACTGCGAAGCGAAAAAGCGGTCCGAAAAGGCGGAAATCGGTGTAAACAGGCGGGAATCGGAT
GCCTGCTGCACGACATCGAA

Downstream 100 bases:

>100_bases
TCCGGTCAGTTCGCCGGCAGCAGCTCCGCCACGGCCTGCCGCGCGATCGCATTCTCCTCGGGCAGCCCGATCGAGATGCG
TAGCCATGTCGCAAAGCCCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAEISDEIGKSGAWRFAVAPMMDWTDRHCRVFHRLLSRRALLYTEMVTTGAVIHGDRTRLLGFDRSEHPVALQLGGSDPA
DLATSARIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAACVAAMKRVVTIPVTVKCRIGIDDQDPEVALDALA
DAVVDAGADALIVHARKAWLNGLSPKENRDIPPLDYDRVYRLKARLPRVPVIINGGITSIEAAQDHLAHVDGVMLGRAAY
QEPWRLLDVDPVLFGEAGPQRSMKDALAALEPYIERQVAQGMRLHAITRHLVGAYHAVPGARAFRRHLAERGVKPDAGIE
VLREAVAMIEERAPSVALA

Sequences:

>Translated_339_residues
MAEISDEIGKSGAWRFAVAPMMDWTDRHCRVFHRLLSRRALLYTEMVTTGAVIHGDRTRLLGFDRSEHPVALQLGGSDPA
DLATSARIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAACVAAMKRVVTIPVTVKCRIGIDDQDPEVALDALA
DAVVDAGADALIVHARKAWLNGLSPKENRDIPPLDYDRVYRLKARLPRVPVIINGGITSIEAAQDHLAHVDGVMLGRAAY
QEPWRLLDVDPVLFGEAGPQRSMKDALAALEPYIERQVAQGMRLHAITRHLVGAYHAVPGARAFRRHLAERGVKPDAGIE
VLREAVAMIEERAPSVALA
>Mature_338_residues
AEISDEIGKSGAWRFAVAPMMDWTDRHCRVFHRLLSRRALLYTEMVTTGAVIHGDRTRLLGFDRSEHPVALQLGGSDPAD
LATSARIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAEPALVAACVAAMKRVVTIPVTVKCRIGIDDQDPEVALDALAD
AVVDAGADALIVHARKAWLNGLSPKENRDIPPLDYDRVYRLKARLPRVPVIINGGITSIEAAQDHLAHVDGVMLGRAAYQ
EPWRLLDVDPVLFGEAGPQRSMKDALAALEPYIERQVAQGMRLHAITRHLVGAYHAVPGARAFRRHLAERGVKPDAGIEV
LREAVAMIEERAPSVALA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=284, Percent_Identity=28.8732394366197, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=57.6802507836991, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789660, Length=300, Percent_Identity=27.6666666666667, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788462, Length=178, Percent_Identity=34.2696629213483, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=203, Percent_Identity=29.064039408867, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=248, Percent_Identity=27.4193548387097, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24580595, Length=256, Percent_Identity=24.609375, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19920448, Length=256, Percent_Identity=24.609375, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921524, Length=287, Percent_Identity=24.390243902439, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24585320, Length=193, Percent_Identity=32.1243523316062, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36963; Mature: 36832

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEISDEIGKSGAWRFAVAPMMDWTDRHCRVFHRLLSRRALLYTEMVTTGAVIHGDRTRL
CCCCCHHHCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEE
LGFDRSEHPVALQLGGSDPADLATSARIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAE
ECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCC
PALVAACVAAMKRVVTIPVTVKCRIGIDDQDPEVALDALADAVVDAGADALIVHARKAWL
HHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
NGLSPKENRDIPPLDYDRVYRLKARLPRVPVIINGGITSIEAAQDHLAHVDGVMLGRAAY
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
QEPWRLLDVDPVLFGEAGPQRSMKDALAALEPYIERQVAQGMRLHAITRHLVGAYHAVPG
HCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
ARAFRRHLAERGVKPDAGIEVLREAVAMIEERAPSVALA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEISDEIGKSGAWRFAVAPMMDWTDRHCRVFHRLLSRRALLYTEMVTTGAVIHGDRTRL
CCCCHHHCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCEEE
LGFDRSEHPVALQLGGSDPADLATSARIGEDFGYDEINLNVGCPSDRVKDGRFGACLMAE
ECCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCEEECCC
PALVAACVAAMKRVVTIPVTVKCRIGIDDQDPEVALDALADAVVDAGADALIVHARKAWL
HHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
NGLSPKENRDIPPLDYDRVYRLKARLPRVPVIINGGITSIEAAQDHLAHVDGVMLGRAAY
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
QEPWRLLDVDPVLFGEAGPQRSMKDALAALEPYIERQVAQGMRLHAITRHLVGAYHAVPG
HCCCEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
ARAFRRHLAERGVKPDAGIEVLREAVAMIEERAPSVALA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]