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The map label for this gene is oprM [H]

Identifier: 146341629

GI number: 146341629

Start: 4921025

End: 4922596

Strand: Direct

Name: oprM [H]

Synonym: BRADO4732

Alternate gene names: 146341629

Gene position: 4921025-4922596 (Clockwise)

Preceding gene: 146341628

Following gene: 146341634

Centisome position: 66.0

GC content: 70.1

Gene sequence:

>1572_bases
ATGCAGCAGCATGATCGTGTCCCATCCCGCGGCTTGCGTACCGCGCACTTTTGTCTGGCGCTGGCGCTCGCGATCGCCGC
GAGCGGCTGCGCGGTCGGTCCGGATTTCATCACCCCCGCGCCGCCGGCCGTCGGCGGATATACGCGGGAGCCGTTGGCGT
CGCCCGCGGTCGACCGCTCCGGTCCGCGTGTCCCGGCGCAGCATTTCGAGGCGGGAGCGGATGTGCCGGCGCGCTGGTGG
GCGGCGTTCCGCTCGGCCCCGCTCAACACGCTGATCCGCCGATCCGTGGCACACAACCCGACGCTCGACGCCGCCGATGC
CGCGATCCGCATCGCAGCCGCAAACGCGCTGGCCCAGCGCGGGCTGTTCTATCCCCAGCTCGGCGCGAACTACACGGCCG
CCGACCAGCAGACCTCGAACATGGGCACGCAGCAGGGACCGGCCGATCCCGCGCAGTCGCGCTACACGCTGCACACCGCC
CAGCTCACCATCAGCTTCGTGCCTGATCTGTGGGGCCAGAACTTGCGCAGCGTGGAAAACCTGGAGGCGCAGGCCGAGCA
GGCGCAATTCCAGCTTGAGGCGGCCTATCTGACGCTGACGGCCAACGTCGTCACGGCGGCGATCCAGGAGGCCTCGCTGC
GCGGCCAGATCGCAGCGACCGAGCGCATCATCAAAATCGAGCGCGACATCCTCGGCATCCTCCAGAAGCAATTGCAGGCC
GGCCAGGCCGCGCAGGTCGACGTGCTGACGCAGCAGACCGCGCTGGCGCAGGTGGAGCAGACCTTGCCGCCGCTGCAGAA
GCAGCTCGCGATCCAGCGGGACCTGCTGACGGCGCTCGCCGGCCAGTTCTCCGCCGACGAGATCACCGAGCGGTTCGACC
TGCAGCATCTCGCTTTGACACGCAATTTGCCGATCAGCCTGCCCGCGACACTGGTGGCGCAGCGGCCCGACGTGCGTGCG
ACTGAGGCCAATCTGCACGCGGCCAGCGCGCTGGTCGGCGTGGCAATCGCGGCGCGGCTGCCCAACATCACGCTGTCGGC
CAATCCCGGATCGAGCGCCTTCAAGGCGGCGGAGCTGTTCACGCCGGGCACGCTGTTCTACACCGTCGCGGGCAGCGCCA
CCGCGACGGTGTTCGACGGCCTCACGCTGTATCATCGGCAGAAAGCGGCGGAGGCCGGGCTCGACCAGGCCGGCGCGCTG
TACCGCCAGGCCGTGATCACGGCGCTGCAGAACGTCGCCGACAGCCTGCGCAGCCTGCAGGCGGACGCGCGCGCGGTGCA
GGCCGCCATCAAGGCCGAGGACAGCGCTCGCGCCACACTCGACATCGTCCAGAAACAGCTGAGCCTCGGGCAGGTCAGCC
AGGTCACGGTGCTCAACGCGCAGCAGGCTTATCTCAACACGGCGATCGCCCGCGTCCAGGCCGAGGCGGCGCGATTGACC
GATACCGCGGCGCTGTTCATGGCGCTCGGCGGCGGATGGCCCGCCGCCTGCACCACGCCGGTGTGGCGCGACTGCGTGAT
GAGCGACGGCCGCCGCGATGGCCTGCGAACGGCGGACGCCGACGCGAGATGA

Upstream 100 bases:

>100_bases
CCGGCGCTACGCCGCATCTTCCTGGCGCACGACCATACGATGGCCCCGGTCGATGACGGCACCACGGCCTCTCCCGAGCC
CGCGGAATAGGAGCCGGCCG

Downstream 100 bases:

>100_bases
TCGGCCGGCACGCCGCGCGCGCCGCCCATCGTCTCAGCTCTGTGAGCGCCTTGCCATGAAGTTGTCGTAGCCGACCTCGG
CGACCTGGAACCAGGAATAG

Product: putative outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MQQHDRVPSRGLRTAHFCLALALAIAASGCAVGPDFITPAPPAVGGYTREPLASPAVDRSGPRVPAQHFEAGADVPARWW
AAFRSAPLNTLIRRSVAHNPTLDAADAAIRIAAANALAQRGLFYPQLGANYTAADQQTSNMGTQQGPADPAQSRYTLHTA
QLTISFVPDLWGQNLRSVENLEAQAEQAQFQLEAAYLTLTANVVTAAIQEASLRGQIAATERIIKIERDILGILQKQLQA
GQAAQVDVLTQQTALAQVEQTLPPLQKQLAIQRDLLTALAGQFSADEITERFDLQHLALTRNLPISLPATLVAQRPDVRA
TEANLHAASALVGVAIAARLPNITLSANPGSSAFKAAELFTPGTLFYTVAGSATATVFDGLTLYHRQKAAEAGLDQAGAL
YRQAVITALQNVADSLRSLQADARAVQAAIKAEDSARATLDIVQKQLSLGQVSQVTVLNAQQAYLNTAIARVQAEAARLT
DTAALFMALGGGWPAACTTPVWRDCVMSDGRRDGLRTADADAR

Sequences:

>Translated_523_residues
MQQHDRVPSRGLRTAHFCLALALAIAASGCAVGPDFITPAPPAVGGYTREPLASPAVDRSGPRVPAQHFEAGADVPARWW
AAFRSAPLNTLIRRSVAHNPTLDAADAAIRIAAANALAQRGLFYPQLGANYTAADQQTSNMGTQQGPADPAQSRYTLHTA
QLTISFVPDLWGQNLRSVENLEAQAEQAQFQLEAAYLTLTANVVTAAIQEASLRGQIAATERIIKIERDILGILQKQLQA
GQAAQVDVLTQQTALAQVEQTLPPLQKQLAIQRDLLTALAGQFSADEITERFDLQHLALTRNLPISLPATLVAQRPDVRA
TEANLHAASALVGVAIAARLPNITLSANPGSSAFKAAELFTPGTLFYTVAGSATATVFDGLTLYHRQKAAEAGLDQAGAL
YRQAVITALQNVADSLRSLQADARAVQAAIKAEDSARATLDIVQKQLSLGQVSQVTVLNAQQAYLNTAIARVQAEAARLT
DTAALFMALGGGWPAACTTPVWRDCVMSDGRRDGLRTADADAR
>Mature_523_residues
MQQHDRVPSRGLRTAHFCLALALAIAASGCAVGPDFITPAPPAVGGYTREPLASPAVDRSGPRVPAQHFEAGADVPARWW
AAFRSAPLNTLIRRSVAHNPTLDAADAAIRIAAANALAQRGLFYPQLGANYTAADQQTSNMGTQQGPADPAQSRYTLHTA
QLTISFVPDLWGQNLRSVENLEAQAEQAQFQLEAAYLTLTANVVTAAIQEASLRGQIAATERIIKIERDILGILQKQLQA
GQAAQVDVLTQQTALAQVEQTLPPLQKQLAIQRDLLTALAGQFSADEITERFDLQHLALTRNLPISLPATLVAQRPDVRA
TEANLHAASALVGVAIAARLPNITLSANPGSSAFKAAELFTPGTLFYTVAGSATATVFDGLTLYHRQKAAEAGLDQAGAL
YRQAVITALQNVADSLRSLQADARAVQAAIKAEDSARATLDIVQKQLSLGQVSQVTVLNAQQAYLNTAIARVQAEAARLT
DTAALFMALGGGWPAACTTPVWRDCVMSDGRRDGLRTADADAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=483, Percent_Identity=24.6376811594203, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55458; Mature: 55458

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQHDRVPSRGLRTAHFCLALALAIAASGCAVGPDFITPAPPAVGGYTREPLASPAVDRS
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPRVPAQHFEAGADVPARWWAAFRSAPLNTLIRRSVAHNPTLDAADAAIRIAAANALAQR
CCCCCHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
GLFYPQLGANYTAADQQTSNMGTQQGPADPAQSRYTLHTAQLTISFVPDLWGQNLRSVEN
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHCHHHHHHHH
LEAQAEQAQFQLEAAYLTLTANVVTAAIQEASLRGQIAATERIIKIERDILGILQKQLQA
HHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
GQAAQVDVLTQQTALAQVEQTLPPLQKQLAIQRDLLTALAGQFSADEITERFDLQHLALT
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RNLPISLPATLVAQRPDVRATEANLHAASALVGVAIAARLPNITLSANPGSSAFKAAELF
CCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHC
TPGTLFYTVAGSATATVFDGLTLYHRQKAAEAGLDQAGALYRQAVITALQNVADSLRSLQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADARAVQAAIKAEDSARATLDIVQKQLSLGQVSQVTVLNAQQAYLNTAIARVQAEAARLT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
DTAALFMALGGGWPAACTTPVWRDCVMSDGRRDGLRTADADAR
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQHDRVPSRGLRTAHFCLALALAIAASGCAVGPDFITPAPPAVGGYTREPLASPAVDRS
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPRVPAQHFEAGADVPARWWAAFRSAPLNTLIRRSVAHNPTLDAADAAIRIAAANALAQR
CCCCCHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
GLFYPQLGANYTAADQQTSNMGTQQGPADPAQSRYTLHTAQLTISFVPDLWGQNLRSVEN
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEEEEEEECCHHHHCHHHHHHHH
LEAQAEQAQFQLEAAYLTLTANVVTAAIQEASLRGQIAATERIIKIERDILGILQKQLQA
HHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
GQAAQVDVLTQQTALAQVEQTLPPLQKQLAIQRDLLTALAGQFSADEITERFDLQHLALT
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RNLPISLPATLVAQRPDVRATEANLHAASALVGVAIAARLPNITLSANPGSSAFKAAELF
CCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHC
TPGTLFYTVAGSATATVFDGLTLYHRQKAAEAGLDQAGALYRQAVITALQNVADSLRSLQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADARAVQAAIKAEDSARATLDIVQKQLSLGQVSQVTVLNAQQAYLNTAIARVQAEAARLT
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
DTAALFMALGGGWPAACTTPVWRDCVMSDGRRDGLRTADADAR
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]