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The map label for this gene is glxR [C]

Identifier: 146341634

GI number: 146341634

Start: 4927144

End: 4928013

Strand: Direct

Name: glxR [C]

Synonym: BRADO4737

Alternate gene names: 146341634

Gene position: 4927144-4928013 (Clockwise)

Preceding gene: 146341629

Following gene: 146341636

Centisome position: 66.08

GC content: 64.48

Gene sequence:

>870_bases
ATGGCTAAAGTCGCTTTCATCGGTCTCGGCGTCATGGGTTTCCCCATGGCGGGACATCTCGTGAAGAAGGGTGGTCACGA
GGTGACGGTGTACAACCGCACCGCGGCCAAGGCGAAGGAATGGGCCGACAAGTTCGGCGGCAAGACAGCTCCGACGCCTA
AGCAGGCCGCCGAGGGCCAGGATTTCGTGATGTGCTGCGTCGGCAACGACAACGATCTGCGCTCGGTGACGATCGGTCCC
GACGGCGCCTTCGGCGGCATGACCAAGGGCGCAACATTCGTCGACCACACCACGGCGTCAGCTGAAGTGGCACGCGAGCT
CGACGCCGCAGCCACCAAGGCTGGCTTCAAATTCGTCGATGCGCCGGTGTCGGGCGGCCAGGCCGGCGCCGAGAACGGCG
TGCTCACCGTCATGTGCGGCGGCACCCAGGACGCCTATGCCGGCGCCGAGCCGGTGATCGCGGCTTATGCGCGCATGTGC
AAGCTGCTCGGGCCGGCCGGCTCAGGCCAGCTGACCAAGATGGTCAACCAGATCTGCATCGCCGGCCTCGTGCAGGGCCT
CTCCGAAGGCATCCACTTCGCCAAGAAGTCGGGCCTCGACGTCCCCGCCGTCATCGAGACCATCTCCAAGGGTGCCGCGC
AGTCCTGGCAGATGGAGAACCGCTACAAGACCATGAACGACGGCAAATACGATTTTGGTTTCGCTGTCGAATGGATGCGG
AAGGACCTGTCGATCTGCCTCAACGAAGCCCGTCGCAACGGCGCCCATTTGCCGGTGACCGCGCTGGTCGACCAGTTCTA
CTCCGAAGTCGAGAAGCTCGGCGGCAAGCGCTGGGACACGTCGAGCCTGCTGGCGCGGCTGGAGAAGTGA

Upstream 100 bases:

>100_bases
AGCCCGCCGCGCCCCTGCGCCGGATGCTGAGGTGTTCCATTCAAGAACAGCCCGGCCCGATCGAGGCCGTCGGCAGAACC
CCAAGGGCAAGGTAGCTCAC

Downstream 100 bases:

>100_bases
GCCGGCACGGGCCGCCGAGATCGGAAAACGATGGCCGCCGCTTGCGGCGGCCATCGGGTCCATTCCGCTACTTGAAGCGG
ATGATGTAGTCGACGTTGAT

Product: putative oxidoreductase 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAKVAFIGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKEWADKFGGKTAPTPKQAAEGQDFVMCCVGNDNDLRSVTIGP
DGAFGGMTKGATFVDHTTASAEVARELDAAATKAGFKFVDAPVSGGQAGAENGVLTVMCGGTQDAYAGAEPVIAAYARMC
KLLGPAGSGQLTKMVNQICIAGLVQGLSEGIHFAKKSGLDVPAVIETISKGAAQSWQMENRYKTMNDGKYDFGFAVEWMR
KDLSICLNEARRNGAHLPVTALVDQFYSEVEKLGGKRWDTSSLLARLEK

Sequences:

>Translated_289_residues
MAKVAFIGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKEWADKFGGKTAPTPKQAAEGQDFVMCCVGNDNDLRSVTIGP
DGAFGGMTKGATFVDHTTASAEVARELDAAATKAGFKFVDAPVSGGQAGAENGVLTVMCGGTQDAYAGAEPVIAAYARMC
KLLGPAGSGQLTKMVNQICIAGLVQGLSEGIHFAKKSGLDVPAVIETISKGAAQSWQMENRYKTMNDGKYDFGFAVEWMR
KDLSICLNEARRNGAHLPVTALVDQFYSEVEKLGGKRWDTSSLLARLEK
>Mature_288_residues
AKVAFIGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKEWADKFGGKTAPTPKQAAEGQDFVMCCVGNDNDLRSVTIGPD
GAFGGMTKGATFVDHTTASAEVARELDAAATKAGFKFVDAPVSGGQAGAENGVLTVMCGGTQDAYAGAEPVIAAYARMCK
LLGPAGSGQLTKMVNQICIAGLVQGLSEGIHFAKKSGLDVPAVIETISKGAAQSWQMENRYKTMNDGKYDFGFAVEWMRK
DLSICLNEARRNGAHLPVTALVDQFYSEVEKLGGKRWDTSSLLARLEK

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=28.6689419795222, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=24.5551601423488, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=30.7692307692308, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI145693186, Length=289, Percent_Identity=31.4878892733564, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1790315, Length=289, Percent_Identity=28.0276816608997, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=26.1016949152542, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=276, Percent_Identity=27.536231884058, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24655230, Length=302, Percent_Identity=28.1456953642384, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922568, Length=302, Percent_Identity=28.1456953642384, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28574115, Length=282, Percent_Identity=22.3404255319149, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30521; Mature: 30390

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKEWADKFGGKTAPTPKQAAEGQ
CCEEEEEECCHHHCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCCC
DFVMCCVGNDNDLRSVTIGPDGAFGGMTKGATFVDHTTASAEVARELDAAATKAGFKFVD
CEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEE
APVSGGQAGAENGVLTVMCGGTQDAYAGAEPVIAAYARMCKLLGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGLVQGLSEGIHFAKKSGLDVPAVIETISKGAAQSWQMENRYKTMNDGKYDFGFAVEWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KDLSICLNEARRNGAHLPVTALVDQFYSEVEKLGGKRWDTSSLLARLEK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AKVAFIGLGVMGFPMAGHLVKKGGHEVTVYNRTAAKAKEWADKFGGKTAPTPKQAAEGQ
CEEEEEECCHHHCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHCCCC
DFVMCCVGNDNDLRSVTIGPDGAFGGMTKGATFVDHTTASAEVARELDAAATKAGFKFVD
CEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEE
APVSGGQAGAENGVLTVMCGGTQDAYAGAEPVIAAYARMCKLLGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AGLVQGLSEGIHFAKKSGLDVPAVIETISKGAAQSWQMENRYKTMNDGKYDFGFAVEWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
KDLSICLNEARRNGAHLPVTALVDQFYSEVEKLGGKRWDTSSLLARLEK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA