Click here to switch to the map view.

The map label for this gene is mexA [H]

Identifier: 146341526

GI number: 146341526

Start: 4814256

End: 4815437

Strand: Reverse

Name: mexA [H]

Synonym: BRADO4623

Alternate gene names: 146341526

Gene position: 4815437-4814256 (Counterclockwise)

Preceding gene: 146341530

Following gene: 146341525

Centisome position: 64.58

GC content: 68.44

Gene sequence:

>1182_bases
ATGCTGAGAGATAAGTTGATCCGTTCCGCTGGCGCGACAATCTGCGCCGTCTCCGTGATGACGCTTGCGGCTTGCACAGA
CCAGGGCGGCACAGCTGCGCCGCATGCGTCCAATGCGCCCGAGGTTGGCGTCGTCAAGCTCGAATCGGCGCCGGTGACGT
TGACGAAGGATCTCCCGGGACGCATCACCTCGATGCGCATCGCCGAGGTTCGGCCGCGCGTCAGCGGCATCGTGATCGAG
CGCAGCTTCCAGCAGGGCGGCATGGTGGACGCCGGCGCGGTGCTGTACCGCATCGATCCGGCCCCGTTCGAGGTCGAGCT
GGAGAAGGCGCGCGCCGCACTCGCCAAGGCGGAGGCCGTGCTCTACCAGGCGGACCGTCAGGAAGATCGCCTGAAGAACC
TGTTGAAGGGCGAGGCCACCACCCAGGCGCAGTATGAGCTCGCGGTCGCCGCAGAGCGCCAGGCGCAGGCCGACGTCGCT
GCCCAGAAAGCGGCCGTCGCGCAGGCGCAGCTCAATCTCGACTGGGCCACGGTGCGGGCGCCGATCTCCGGACGCATCGG
GCGGGCGCTGGTGACCGAGGGCGCGCTGGTCAATCCGAACGAGACGACGCATCTCGCCACCATCCAGCAGCTCGATCCCG
TCTATGCCGACTTCACGCAATCCGTGGACGAGCTGAATCAGCTGCGGCGCGATCTCGCCGAAGGCCGCCTGAAGAGCGTG
TCGCGGGAGGCGGCGCGCGTCCGCCTGCTGTTCGATGACGGCTCGATGTACAAACATCCCGGCCGGCTGTTGTTCTCCGA
CGTCAGCGTCGATCCGGGCACCGCGAAGGTGACCCTGCGCGGCGAGTTCCCCAATCCCGAGGGCGAACTGCTTCCGGGCA
TGTATGTGCGGGTCCAGATCGAGGAGGCGATCGACGCTGCGGGCCTCGTCGTGCCGTCGCAGGCCGTGCAGCGGACGACG
GCCGGCGGCAGCGCTGTCTACGTTGTCAACAATGAGGGACGCGCGAATCTTCAGCCGGTGCGGCTCGGACGCGCGCTGAA
CGGCGGCATGCTGGTCGAGGACGGTCTGAAGCCGGGCTGGCGCGTCGTGGTCGACGGCTTCCAGAAGTTCGCGCCCGGCA
GTGCCGTCACGCCGGTGCCGTGGCAGGCCGCCGGCGCCGGCAAGCCGTCCGCCTCGAATTGA

Upstream 100 bases:

>100_bases
TGAGCGACGATCCGGAGACGGGAGTGGATCGAGCGTATTTGCTCTGTCCGGCCCGATCATCGGGCAATGATGCGCGCCGC
GATGTTATTCGATGGGCTTC

Downstream 100 bases:

>100_bases
ACGCGGCCACGGCCCGGAGACCATCTGTCATGTCACGCTTCTTCATCGCACGTCCGATCTTTGCCTGGGTCGTGGCGATC
TTCATCTGTCTCGGCGGAAT

Product: AcrB/AcrD/AcrF family mulitdrug efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLRDKLIRSAGATICAVSVMTLAACTDQGGTAAPHASNAPEVGVVKLESAPVTLTKDLPGRITSMRIAEVRPRVSGIVIE
RSFQQGGMVDAGAVLYRIDPAPFEVELEKARAALAKAEAVLYQADRQEDRLKNLLKGEATTQAQYELAVAAERQAQADVA
AQKAAVAQAQLNLDWATVRAPISGRIGRALVTEGALVNPNETTHLATIQQLDPVYADFTQSVDELNQLRRDLAEGRLKSV
SREAARVRLLFDDGSMYKHPGRLLFSDVSVDPGTAKVTLRGEFPNPEGELLPGMYVRVQIEEAIDAAGLVVPSQAVQRTT
AGGSAVYVVNNEGRANLQPVRLGRALNGGMLVEDGLKPGWRVVVDGFQKFAPGSAVTPVPWQAAGAGKPSASN

Sequences:

>Translated_393_residues
MLRDKLIRSAGATICAVSVMTLAACTDQGGTAAPHASNAPEVGVVKLESAPVTLTKDLPGRITSMRIAEVRPRVSGIVIE
RSFQQGGMVDAGAVLYRIDPAPFEVELEKARAALAKAEAVLYQADRQEDRLKNLLKGEATTQAQYELAVAAERQAQADVA
AQKAAVAQAQLNLDWATVRAPISGRIGRALVTEGALVNPNETTHLATIQQLDPVYADFTQSVDELNQLRRDLAEGRLKSV
SREAARVRLLFDDGSMYKHPGRLLFSDVSVDPGTAKVTLRGEFPNPEGELLPGMYVRVQIEEAIDAAGLVVPSQAVQRTT
AGGSAVYVVNNEGRANLQPVRLGRALNGGMLVEDGLKPGWRVVVDGFQKFAPGSAVTPVPWQAAGAGKPSASN
>Mature_393_residues
MLRDKLIRSAGATICAVSVMTLAACTDQGGTAAPHASNAPEVGVVKLESAPVTLTKDLPGRITSMRIAEVRPRVSGIVIE
RSFQQGGMVDAGAVLYRIDPAPFEVELEKARAALAKAEAVLYQADRQEDRLKNLLKGEATTQAQYELAVAAERQAQADVA
AQKAAVAQAQLNLDWATVRAPISGRIGRALVTEGALVNPNETTHLATIQQLDPVYADFTQSVDELNQLRRDLAEGRLKSV
SREAARVRLLFDDGSMYKHPGRLLFSDVSVDPGTAKVTLRGEFPNPEGELLPGMYVRVQIEEAIDAAGLVVPSQAVQRTT
AGGSAVYVVNNEGRANLQPVRLGRALNGGMLVEDGLKPGWRVVVDGFQKFAPGSAVTPVPWQAAGAGKPSASN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=364, Percent_Identity=42.032967032967, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1786668, Length=374, Percent_Identity=42.5133689839572, Blast_Score=267, Evalue=7e-73,
Organism=Escherichia coli, GI1789929, Length=352, Percent_Identity=39.4886363636364, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI87082036, Length=330, Percent_Identity=29.6969696969697, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87081951, Length=132, Percent_Identity=31.8181818181818, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41773; Mature: 41773

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRDKLIRSAGATICAVSVMTLAACTDQGGTAAPHASNAPEVGVVKLESAPVTLTKDLPG
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCC
RITSMRIAEVRPRVSGIVIERSFQQGGMVDAGAVLYRIDPAPFEVELEKARAALAKAEAV
CHHEEEHHHHCCCCEEEEEEECCCCCCCEECCEEEEEECCCCCEEEHHHHHHHHHHHHHH
LYQADRQEDRLKNLLKGEATTQAQYELAVAAERQAQADVAAQKAAVAQAQLNLDWATVRA
HHHHCCHHHHHHHHHCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PISGRIGRALVTEGALVNPNETTHLATIQQLDPVYADFTQSVDELNQLRRDLAEGRLKSV
CCCCCCCHHHHCCCCEECCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SREAARVRLLFDDGSMYKHPGRLLFSDVSVDPGTAKVTLRGEFPNPEGELLPGMYVRVQI
HHCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEE
EEAIDAAGLVVPSQAVQRTTAGGSAVYVVNNEGRANLQPVRLGRALNGGMLVEDGLKPGW
HHHHCCCCEECCCHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCCCCEEEECCCCCCH
RVVVDGFQKFAPGSAVTPVPWQAAGAGKPSASN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRDKLIRSAGATICAVSVMTLAACTDQGGTAAPHASNAPEVGVVKLESAPVTLTKDLPG
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCC
RITSMRIAEVRPRVSGIVIERSFQQGGMVDAGAVLYRIDPAPFEVELEKARAALAKAEAV
CHHEEEHHHHCCCCEEEEEEECCCCCCCEECCEEEEEECCCCCEEEHHHHHHHHHHHHHH
LYQADRQEDRLKNLLKGEATTQAQYELAVAAERQAQADVAAQKAAVAQAQLNLDWATVRA
HHHHCCHHHHHHHHHCCCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PISGRIGRALVTEGALVNPNETTHLATIQQLDPVYADFTQSVDELNQLRRDLAEGRLKSV
CCCCCCCHHHHCCCCEECCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SREAARVRLLFDDGSMYKHPGRLLFSDVSVDPGTAKVTLRGEFPNPEGELLPGMYVRVQI
HHCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEE
EEAIDAAGLVVPSQAVQRTTAGGSAVYVVNNEGRANLQPVRLGRALNGGMLVEDGLKPGW
HHHHCCCCEECCCHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCCCCEEEECCCCCCH
RVVVDGFQKFAPGSAVTPVPWQAAGAGKPSASN
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]