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The map label for this gene is dhaK [H]

Identifier: 146341512

GI number: 146341512

Start: 4798612

End: 4799601

Strand: Reverse

Name: dhaK [H]

Synonym: BRADO4609

Alternate gene names: 146341512

Gene position: 4799601-4798612 (Counterclockwise)

Preceding gene: 146341513

Following gene: 146341510

Centisome position: 64.37

GC content: 66.57

Gene sequence:

>990_bases
ATGAAGAAGCTGATCAACGCGGTCGACGATGTGCTGACCGAGAGCCTGCAGGGCTTTGCCGCCATTCATGGCGACATCGT
CACGCTCGGCGACGAGAGCAAGTTCGTCCGCCGCAAAAGTCTCAAGCCGGGCAAGGTCGCGCTGATCTCCGGCGGTGGAT
CCGGCCATGAGCCGCTGCATGCCGGCTTTGTCGGACAAGGCATGCTGGACGCCGCCTGTCCGGGCCAGGTCTTCACCTCG
CCGACGCCCGACCAGATGCTCGCCGCAGCGCAGGCCGTCGAGACCGGCGGCGGCTCGCTGTTCATCGTCAAGAACTACGA
AGGCGATGTCATGAATTTCGCGATGGCCGCGGAGATGATGGACGCGAAAGTCGCGACTGTCATCACCGATGACGACGTCG
CGGTGGAGACGTCGACCTATTCGACCGGCCGCCGCGGCGTCGCCGGCACGCTCGTCGTCGAAAAGATTCTCGGCGCCGCC
GCCGAGCAGGGCCGCGATCTCGACGCGCTGAAGGCGCTCGGCGACGACGTGAACAAGCGCACCCGCTCAATGGGCGTCGC
GTTGACCTCGTGCACCGTGCCGGCAGCCGGCAAGCCGACCTTCGCTCTGGGTGAGGACGAGATGGAGATGGGAGTCGGCA
TCCATGGCGAGCCAGGACGCCGGCGCGTCAAGCTCGCGCCGGCCGATGCGATCGCCGAGGAGATGATGAGCGCGATCCTG
GCCGATCTCGCGGCCAAGCCGGGTACGCCATGCCTGCTGCTGGTCAACGGCTTCGGCGGCACGCCGGCGATGGAGCTCTA
TCTGATGACCAATTCAGCGCGCCGGATCCTTGAAGGACGAGGGCTCACGATCGCGCGCGGCCTGGTCGGCTCTTATGTCA
CCTCGCTCGACATGGCCGGATGCTCGCTCACCGTGACAGCGCTCGACGATGCAACGCTCCCGCTGTGGGATGCGCCGGTG
CACACGGCGGCGTTGCGCTGGGGCTGCTGA

Upstream 100 bases:

>100_bases
ACGCCCATGCCGACCGCATCATCACGGGTGTCGCGCGCGGCATCACCGCCCTGATCTCAGCCAATGAAAACAATGACGAT
GCCGAACCGGGAGGGACGCC

Downstream 100 bases:

>100_bases
GCGGACCGCTGCTCAGCGGATCTCGTTCGAATAATAATGATGCGCCGTCGTCGCGCGGTGCTGGCTCAGGATCGCGAGTG
CGCCGGACTGGTCGTAGGCC

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKKLINAVDDVLTESLQGFAAIHGDIVTLGDESKFVRRKSLKPGKVALISGGGSGHEPLHAGFVGQGMLDAACPGQVFTS
PTPDQMLAAAQAVETGGGSLFIVKNYEGDVMNFAMAAEMMDAKVATVITDDDVAVETSTYSTGRRGVAGTLVVEKILGAA
AEQGRDLDALKALGDDVNKRTRSMGVALTSCTVPAAGKPTFALGEDEMEMGVGIHGEPGRRRVKLAPADAIAEEMMSAIL
ADLAAKPGTPCLLLVNGFGGTPAMELYLMTNSARRILEGRGLTIARGLVGSYVTSLDMAGCSLTVTALDDATLPLWDAPV
HTAALRWGC

Sequences:

>Translated_329_residues
MKKLINAVDDVLTESLQGFAAIHGDIVTLGDESKFVRRKSLKPGKVALISGGGSGHEPLHAGFVGQGMLDAACPGQVFTS
PTPDQMLAAAQAVETGGGSLFIVKNYEGDVMNFAMAAEMMDAKVATVITDDDVAVETSTYSTGRRGVAGTLVVEKILGAA
AEQGRDLDALKALGDDVNKRTRSMGVALTSCTVPAAGKPTFALGEDEMEMGVGIHGEPGRRRVKLAPADAIAEEMMSAIL
ADLAAKPGTPCLLLVNGFGGTPAMELYLMTNSARRILEGRGLTIARGLVGSYVTSLDMAGCSLTVTALDDATLPLWDAPV
HTAALRWGC
>Mature_329_residues
MKKLINAVDDVLTESLQGFAAIHGDIVTLGDESKFVRRKSLKPGKVALISGGGSGHEPLHAGFVGQGMLDAACPGQVFTS
PTPDQMLAAAQAVETGGGSLFIVKNYEGDVMNFAMAAEMMDAKVATVITDDDVAVETSTYSTGRRGVAGTLVVEKILGAA
AEQGRDLDALKALGDDVNKRTRSMGVALTSCTVPAAGKPTFALGEDEMEMGVGIHGEPGRRRVKLAPADAIAEEMMSAIL
ADLAAKPGTPCLLLVNGFGGTPAMELYLMTNSARRILEGRGLTIARGLVGSYVTSLDMAGCSLTVTALDDATLPLWDAPV
HTAALRWGC

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=338, Percent_Identity=42.8994082840237, Blast_Score=238, Evalue=5e-63,
Organism=Escherichia coli, GI87081857, Length=355, Percent_Identity=48.4507042253521, Blast_Score=330, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI17565018, Length=322, Percent_Identity=35.0931677018634, Blast_Score=200, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6321055, Length=303, Percent_Identity=37.953795379538, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323570, Length=328, Percent_Identity=37.5, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34122; Mature: 34122

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLINAVDDVLTESLQGFAAIHGDIVTLGDESKFVRRKSLKPGKVALISGGGSGHEPLH
CHHHHHHHHHHHHHHHHHHEEECCCEEEECCCHHHHHHHCCCCCCEEEEECCCCCCCCCC
AGFVGQGMLDAACPGQVFTSPTPDQMLAAAQAVETGGGSLFIVKNYEGDVMNFAMAAEMM
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
DAKVATVITDDDVAVETSTYSTGRRGVAGTLVVEKILGAAAEQGRDLDALKALGDDVNKR
CCEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TRSMGVALTSCTVPAAGKPTFALGEDEMEMGVGIHGEPGRRRVKLAPADAIAEEMMSAIL
HHHHCEEEEEEECCCCCCCCEEECCCHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHH
ADLAAKPGTPCLLLVNGFGGTPAMELYLMTNSARRILEGRGLTIARGLVGSYVTSLDMAG
HHHHCCCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHCCCCEEHHHHHHHHHHHHHCCC
CSLTVTALDDATLPLWDAPVHTAALRWGC
CEEEEEEECCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKKLINAVDDVLTESLQGFAAIHGDIVTLGDESKFVRRKSLKPGKVALISGGGSGHEPLH
CHHHHHHHHHHHHHHHHHHEEECCCEEEECCCHHHHHHHCCCCCCEEEEECCCCCCCCCC
AGFVGQGMLDAACPGQVFTSPTPDQMLAAAQAVETGGGSLFIVKNYEGDVMNFAMAAEMM
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
DAKVATVITDDDVAVETSTYSTGRRGVAGTLVVEKILGAAAEQGRDLDALKALGDDVNKR
CCEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TRSMGVALTSCTVPAAGKPTFALGEDEMEMGVGIHGEPGRRRVKLAPADAIAEEMMSAIL
HHHHCEEEEEEECCCCCCCCEEECCCHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHH
ADLAAKPGTPCLLLVNGFGGTPAMELYLMTNSARRILEGRGLTIARGLVGSYVTSLDMAG
HHHHCCCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHCCCCEEHHHHHHHHHHHHHCCC
CSLTVTALDDATLPLWDAPVHTAALRWGC
CEEEEEEECCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]