Click here to switch to the map view.

The map label for this gene is nitA [H]

Identifier: 146341448

GI number: 146341448

Start: 4737380

End: 4738393

Strand: Reverse

Name: nitA [H]

Synonym: BRADO4539

Alternate gene names: 146341448

Gene position: 4738393-4737380 (Counterclockwise)

Preceding gene: 146341454

Following gene: 146341445

Centisome position: 63.55

GC content: 66.37

Gene sequence:

>1014_bases
ATGGGACTGGCACATCCGAAATACAAGGTGGCGGTGGTGCAGGCGGCGCCGGCCTGGCTCGACCTCGACGCCTCGATCAA
GAAGACGATCGGATTGATCGAGGAGGCGGCCGACAAGGGCGCCAAGCTGATCGCCTTCCCGGAGGTCTTCATCCCCGGCT
ATCCCTGGCACATCTGGATGGATTCACCGGCCTGGTGCATCGGCCGCGGCTTCGTGCAGCGCTATTTCGACAATTCGCTG
GCCTATGACAGCCCGCAGGCCGAGACGCTGCGCGCCGCCGTGCGCAAGGCGAAGCTGACCGCTGTGCTCGGCATTTCCGA
GCGCGACGGCGGCAGCCTCTACATCGCGCAATGGCTGATCGGCCCGGATGGCGAGACCATCGCCAAGCGCCGCAAGCTGC
GGCCGACCCATGCCGAGCGCACCGTATATGGTGAGGGCGACGGCAGCGATCTCGCCGTACACGATCGTCCGGACATCGGC
CGGCTCGGCGCGCTGTGCTGCTGGGAGCATCTGCAGCCGCTGTCGAAATATGCGATGTATGCCCAAAACGAGCAGGTGCA
TGTTGCGGCCTGGCCGAGCTTCTCGCTGTACGACCCGTTCGCGCCGGCGCTCGGCGCCGAGGTCAACAACGCGGCCTCGC
GGGTCTACGCGGTGGAGGGCTCGTGCTTCGTGCTGGCGCCATGCGCCACCGTCTCGCAGGCCATGATCGACGAATTGTGC
GACCGGCCCGACAAGCACGCGCTGCTGCATGTCGGCGGCGGCCACGCCGTGATCTTCGGTCCGGACGGCTCCTCGCTGGT
CGAGAAGCTGCCGCCGGATCAGGAAGGCCTGCTGATCGCCGAGATCGATCTCGGCATGATCGGCATCGCCAAGAACGCTG
CCGATCCCGCCGGACATTATTCGCGCCCCGACGTGACCCGGTTGCTGCTCAACAAGAAGCCGTTCAATCGCGTCGAGGCG
TTTGCACTACCCGTCGATACTGTGGCGCCGGGCGAGGTCGCGGCTGGCGGGTAG

Upstream 100 bases:

>100_bases
GTTCCTTGCAAGACGAGCGCGAACCGGCACCTCGGTCAATAGTGGCGTGACAGGGAGCGCGGCGGGACCGCCGCAAACGG
CAGCAGCGGGAGCATCGACG

Downstream 100 bases:

>100_bases
CCGGCCGCGAGGCTGCTAGGTCCCGGCTTCGGGCTGCGCCGGCGGCGGTGCCGCACGGCGCGGCGGACGCTGGGCGCGCG
CGACCGGTGCTGCCATCTTC

Product: aliphatic nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MGLAHPKYKVAVVQAAPAWLDLDASIKKTIGLIEEAADKGAKLIAFPEVFIPGYPWHIWMDSPAWCIGRGFVQRYFDNSL
AYDSPQAETLRAAVRKAKLTAVLGISERDGGSLYIAQWLIGPDGETIAKRRKLRPTHAERTVYGEGDGSDLAVHDRPDIG
RLGALCCWEHLQPLSKYAMYAQNEQVHVAAWPSFSLYDPFAPALGAEVNNAASRVYAVEGSCFVLAPCATVSQAMIDELC
DRPDKHALLHVGGGHAVIFGPDGSSLVEKLPPDQEGLLIAEIDLGMIGIAKNAADPAGHYSRPDVTRLLLNKKPFNRVEA
FALPVDTVAPGEVAAGG

Sequences:

>Translated_337_residues
MGLAHPKYKVAVVQAAPAWLDLDASIKKTIGLIEEAADKGAKLIAFPEVFIPGYPWHIWMDSPAWCIGRGFVQRYFDNSL
AYDSPQAETLRAAVRKAKLTAVLGISERDGGSLYIAQWLIGPDGETIAKRRKLRPTHAERTVYGEGDGSDLAVHDRPDIG
RLGALCCWEHLQPLSKYAMYAQNEQVHVAAWPSFSLYDPFAPALGAEVNNAASRVYAVEGSCFVLAPCATVSQAMIDELC
DRPDKHALLHVGGGHAVIFGPDGSSLVEKLPPDQEGLLIAEIDLGMIGIAKNAADPAGHYSRPDVTRLLLNKKPFNRVEA
FALPVDTVAPGEVAAGG
>Mature_336_residues
GLAHPKYKVAVVQAAPAWLDLDASIKKTIGLIEEAADKGAKLIAFPEVFIPGYPWHIWMDSPAWCIGRGFVQRYFDNSLA
YDSPQAETLRAAVRKAKLTAVLGISERDGGSLYIAQWLIGPDGETIAKRRKLRPTHAERTVYGEGDGSDLAVHDRPDIGR
LGALCCWEHLQPLSKYAMYAQNEQVHVAAWPSFSLYDPFAPALGAEVNNAASRVYAVEGSCFVLAPCATVSQAMIDELCD
RPDKHALLHVGGGHAVIFGPDGSSLVEKLPPDQEGLLIAEIDLGMIGIAKNAADPAGHYSRPDVTRLLLNKKPFNRVEAF
ALPVDTVAPGEVAAGG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17557111, Length=318, Percent_Identity=33.0188679245283, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6322027, Length=191, Percent_Identity=30.8900523560209, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 36316; Mature: 36185

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00920 NITRIL_CHT_1 ; PS00921 NITRIL_CHT_2 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAHPKYKVAVVQAAPAWLDLDASIKKTIGLIEEAADKGAKLIAFPEVFIPGYPWHIWM
CCCCCCCEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEE
DSPAWCIGRGFVQRYFDNSLAYDSPQAETLRAAVRKAKLTAVLGISERDGGSLYIAQWLI
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEEE
GPDGETIAKRRKLRPTHAERTVYGEGDGSDLAVHDRPDIGRLGALCCWEHLQPLSKYAMY
CCCCHHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AQNEQVHVAAWPSFSLYDPFAPALGAEVNNAASRVYAVEGSCFVLAPCATVSQAMIDELC
CCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEEECCCEEEEECHHHHHHHHHHHHH
DRPDKHALLHVGGGHAVIFGPDGSSLVEKLPPDQEGLLIAEIDLGMIGIAKNAADPAGHY
CCCCCCEEEEECCCEEEEECCCCHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCC
SRPDVTRLLLNKKPFNRVEAFALPVDTVAPGEVAAGG
CCCHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
GLAHPKYKVAVVQAAPAWLDLDASIKKTIGLIEEAADKGAKLIAFPEVFIPGYPWHIWM
CCCCCCEEEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCEEEEE
DSPAWCIGRGFVQRYFDNSLAYDSPQAETLRAAVRKAKLTAVLGISERDGGSLYIAQWLI
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEEEEE
GPDGETIAKRRKLRPTHAERTVYGEGDGSDLAVHDRPDIGRLGALCCWEHLQPLSKYAMY
CCCCHHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
AQNEQVHVAAWPSFSLYDPFAPALGAEVNNAASRVYAVEGSCFVLAPCATVSQAMIDELC
CCCCEEEEEECCCCCCCCCCCHHHCCCCCCCCCEEEEECCCEEEEECHHHHHHHHHHHHH
DRPDKHALLHVGGGHAVIFGPDGSSLVEKLPPDQEGLLIAEIDLGMIGIAKNAADPAGHY
CCCCCCEEEEECCCEEEEECCCCHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCC
SRPDVTRLLLNKKPFNRVEAFALPVDTVAPGEVAAGG
CCCHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]