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The map label for this gene is smpB [H]

Identifier: 146341386

GI number: 146341386

Start: 4672518

End: 4672991

Strand: Reverse

Name: smpB [H]

Synonym: BRADO4473

Alternate gene names: 146341386

Gene position: 4672991-4672518 (Counterclockwise)

Preceding gene: 146341387

Following gene: 146341385

Centisome position: 62.67

GC content: 62.66

Gene sequence:

>474_bases
GTGGCGAACAAGAACGAGCCGAAGATCACCGTTGCCGCCGAGAACCGCAAGGCGCGGTTCAACTATGCGATCGAGGACAC
GATCGAGGCCGGCATTGCGCTGACCGGCACCGAGGTGAAGTCGGTCCGCGGCGGCAAATCGACGATCGCGGAATCCTATG
CGGATTCCCGCGACGGCGAGATATGGCTGATCAACGCCAACATTCCCGAATATCTGCAGGCCAACCGCTTCAATCACGAG
CCGAAGCGGCCGCGCAAGCTCTTGCTGCATCGCAAGCAGATCAACAAGCTGATGGGCGCGGTCGAGCGTGAGGGCATGAC
GCTGATCCCGCTCAAGCTCTATTTCAACGAGCGTGGCCGCGCCAAGCTGCTGCTGGCTGTCGCCAAGGGCAAGAAGCTGC
ACGACAAGCGCGAGAGCGAGAAGAAGCGCGATTGGGGCCGCGAGAAGGGTCGCCTGCTGCGCGCGCGCGGTTGA

Upstream 100 bases:

>100_bases
CTGAAGCGCCGGCAAGAGGCTGCTCCGGCCGCGCCGCCGAAGCCGAGTGCCGAGGTCGAGCTTCTCACCGAAATCAGGGA
TCTCCTGAAGAAAGCCTGAC

Downstream 100 bases:

>100_bases
CGCGCTGCGAGCCGTTGCGCGCTTGCCTGGGCCGCAGTCTGAACGCCAGAGCCTTGCCTAAACCTTCTTAGTGCCTCAGC
CGTGGGATGCACCGATGACT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MANKNEPKITVAAENRKARFNYAIEDTIEAGIALTGTEVKSVRGGKSTIAESYADSRDGEIWLINANIPEYLQANRFNHE
PKRPRKLLLHRKQINKLMGAVEREGMTLIPLKLYFNERGRAKLLLAVAKGKKLHDKRESEKKRDWGREKGRLLRARG

Sequences:

>Translated_157_residues
MANKNEPKITVAAENRKARFNYAIEDTIEAGIALTGTEVKSVRGGKSTIAESYADSRDGEIWLINANIPEYLQANRFNHE
PKRPRKLLLHRKQINKLMGAVEREGMTLIPLKLYFNERGRAKLLLAVAKGKKLHDKRESEKKRDWGREKGRLLRARG
>Mature_156_residues
ANKNEPKITVAAENRKARFNYAIEDTIEAGIALTGTEVKSVRGGKSTIAESYADSRDGEIWLINANIPEYLQANRFNHEP
KRPRKLLLHRKQINKLMGAVEREGMTLIPLKLYFNERGRAKLLLAVAKGKKLHDKRESEKKRDWGREKGRLLRARG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=151, Percent_Identity=43.7086092715232, Blast_Score=123, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17949; Mature: 17818

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKNEPKITVAAENRKARFNYAIEDTIEAGIALTGTEVKSVRGGKSTIAESYADSRDGE
CCCCCCCEEEEEECCCCEEEEEEHHHHHHCCEEEECCHHHHCCCCHHHHHHHHCCCCCCE
IWLINANIPEYLQANRFNHEPKRPRKLLLHRKQINKLMGAVEREGMTLIPLKLYFNERGR
EEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
AKLLLAVAKGKKLHDKRESEKKRDWGREKGRLLRARG
EEEEEEEECCCCHHHHHHHHHHHHCCHHHCCEEECCC
>Mature Secondary Structure 
ANKNEPKITVAAENRKARFNYAIEDTIEAGIALTGTEVKSVRGGKSTIAESYADSRDGE
CCCCCCEEEEEECCCCEEEEEEHHHHHHCCEEEECCHHHHCCCCHHHHHHHHCCCCCCE
IWLINANIPEYLQANRFNHEPKRPRKLLLHRKQINKLMGAVEREGMTLIPLKLYFNERGR
EEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
AKLLLAVAKGKKLHDKRESEKKRDWGREKGRLLRARG
EEEEEEEECCCCHHHHHHHHHHHHCCHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA