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The map label for this gene is mscL

Identifier: 146341387

GI number: 146341387

Start: 4672993

End: 4673409

Strand: Reverse

Name: mscL

Synonym: BRADO4474

Alternate gene names: 146341387

Gene position: 4673409-4672993 (Counterclockwise)

Preceding gene: 146341388

Following gene: 146341386

Centisome position: 62.67

GC content: 60.43

Gene sequence:

>417_bases
ATGTTGAAGGAATTCCGAGAATTCGCGATGAAGGGCAATGTCGTCGACCTCGCGGTCGGCGTCATCATCGGCGGCGCGTT
CGGCGCCATCGTGACCTCGCTGGTCGGCGACATCATCATGCCGATCATCGGCGCCATCACCGGTGGTTTGGACTTTTCGA
ACTACTTCATTCCGCTCGCCAAGTCGGTCACTGCGACCAACCTGGCGGACGCAAAGAAGCAGGGCGCCGTGCTCGCCTAT
GGCAGCTTCCTGACGCTGACGCTGAACTTCTTCATCGTCGCCTTCGTGCTGTTCATGGTGATCCGCGGCATGAACAAGCT
GAAGCGCCGGCAAGAGGCTGCTCCGGCCGCGCCGCCGAAGCCGAGTGCCGAGGTCGAGCTTCTCACCGAAATCAGGGATC
TCCTGAAGAAAGCCTGA

Upstream 100 bases:

>100_bases
TGTCCGAGCCGACCCGTGTGGCGGTGCGCCAGGCCATGGTGCATGCCGGGCTGATCAACTGAGCTGAGCCGTTTCTTGCA
CGTCTGATGGGGAGACAGAC

Downstream 100 bases:

>100_bases
CGTGGCGAACAAGAACGAGCCGAAGATCACCGTTGCCGCCGAGAACCGCAAGGCGCGGTTCAACTATGCGATCGAGGACA
CGATCGAGGCCGGCATTGCG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MLKEFREFAMKGNVVDLAVGVIIGGAFGAIVTSLVGDIIMPIIGAITGGLDFSNYFIPLAKSVTATNLADAKKQGAVLAY
GSFLTLTLNFFIVAFVLFMVIRGMNKLKRRQEAAPAAPPKPSAEVELLTEIRDLLKKA

Sequences:

>Translated_138_residues
MLKEFREFAMKGNVVDLAVGVIIGGAFGAIVTSLVGDIIMPIIGAITGGLDFSNYFIPLAKSVTATNLADAKKQGAVLAY
GSFLTLTLNFFIVAFVLFMVIRGMNKLKRRQEAAPAAPPKPSAEVELLTEIRDLLKKA
>Mature_138_residues
MLKEFREFAMKGNVVDLAVGVIIGGAFGAIVTSLVGDIIMPIIGAITGGLDFSNYFIPLAKSVTATNLADAKKQGAVLAY
GSFLTLTLNFFIVAFVLFMVIRGMNKLKRRQEAAPAAPPKPSAEVELLTEIRDLLKKA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=139, Percent_Identity=54.6762589928058, Blast_Score=138, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BRASO (A4YWD8)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001206435.1
- ProteinModelPortal:   A4YWD8
- SMR:   A4YWD8
- STRING:   A4YWD8
- GeneID:   5117807
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO4474
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- ProtClustDB:   PRK13952
- BioCyc:   BSP376:BRADO4474-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14760; Mature: 14760

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d37db58)-; HASH(0x1dc25460)-; HASH(0x1de19498)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFREFAMKGNVVDLAVGVIIGGAFGAIVTSLVGDIIMPIIGAITGGLDFSNYFIPLA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KSVTATNLADAKKQGAVLAYGSFLTLTLNFFIVAFVLFMVIRGMNKLKRRQEAAPAAPPK
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSAEVELLTEIRDLLKKA
CCHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLKEFREFAMKGNVVDLAVGVIIGGAFGAIVTSLVGDIIMPIIGAITGGLDFSNYFIPLA
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
KSVTATNLADAKKQGAVLAYGSFLTLTLNFFIVAFVLFMVIRGMNKLKRRQEAAPAAPPK
HHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSAEVELLTEIRDLLKKA
CCHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA