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The map label for this gene is gatA [H]

Identifier: 146341317

GI number: 146341317

Start: 4598798

End: 4600000

Strand: Reverse

Name: gatA [H]

Synonym: BRADO4399

Alternate gene names: 146341317

Gene position: 4600000-4598798 (Counterclockwise)

Preceding gene: 146341318

Following gene: 146341314

Centisome position: 61.69

GC content: 66.75

Gene sequence:

>1203_bases
ATGGTTCAGGACCCCCTCGGAGCCTTCCTTCCCAACGCGATCATGCGGCGTTCGCCGCATCGGTCCGGCCCGTTGTCCGG
ACTGAGCTTCGCGGTCAAGGATCTGTTCGATGTTGCCGGCGATGTGACCGGCTGCGGAAATCCGGACTGGGCCGCCTGTC
ATGACACCCCGGAGAGGGATGCCTGGGCCGTGGACGCGATGTGCTGCGCTGGCGCGACGCTGACCGGCAAGACCATCACC
GATGAGATTTCGCTCGGACTGCTCGGCATCAACAGGTTCTACGGAACGCCGCTCAATCCGCGTGCGCCGGATCGTGTCCC
CGGCGGCTCGTCGAGCGGCTCTGCGTCCGCGGTGGCTGGTGGACTGGTCGACGTCGCATTAGGCACCGACTCCGGCGGCT
CCGTGCGCACGCCCGCGAGCTTCTGCGGGATCTATGGCCTGCGGCCCACGCACGGCCGTATCTCCGTCGCTGGCCTGATG
ACCCAGGCGCCGTCGTTCGACACCGTCGGCTATTTCACGAGAGATGCGCTGACGTTTGGCCGCGTCGGAAGCGTGCTGCT
CGCCGAGCCGATTGCGGATGGGCTGCAGCCGGACATCGTGATTGCTTCAGATTGCTTTGCGCTGGCCGACGAGCCGGTCA
GGGCCGCCTTGCAACCGGTTGTCGCGCGGCTCCGCAGCGTCGCACCAGCCACGGAGGCGGCGCTGGCAGATGGCGAGCTT
CTCGCGTGGGGGCGGCATCAGCGCGTCCTGCAGAAAAGCGAATTTCACGCGACGTTTCGCGATTGGATCGACCGGGTCAA
TCCGCGCTTTTCCTCGGAGGTGGCCGGCGCCTTTGCCGATGACGGACGCATTGCCCCGCAGGATCTCGCAACCGCCGAGG
TCTTCCGCGCGGCCGCATCGAAGCGGCTCGACGATGTGCTGGACGGGCGACGGATGCTGTGTCTGCCGACAACGCCCATT
CTGCCGATCGCTCGCGATGCCGGTCTATCAGAGATGCGCACGGCCGTGCATCGGATCGTCGACCTGACGTGCATCGCCGG
GTTGACGGGGCTTCCGCAGGTGAACCTCCCCGTGGCGACATCCGGTGCGGTCCCTGTCGGACTGTCTCTGATTGGCTGGC
GCGGCGGCGATGCGTCGCTGCTCGCGGCGGCGCAGACGCTTGCGAGAGAGTTCTCGATCGGCTTGTTCGAGGAGCCGTCA
TGA

Upstream 100 bases:

>100_bases
TTTCCGCAAGCTCTACAAAGAGTGACTGCATGCCAGGCGCGCTCACCCGAGCGCGCCTGGCAACAAATCTGGATGCTGCT
TGCGAGTGATCGGGAGAACC

Downstream 100 bases:

>100_bases
CCGACGTCGATGACACACGCGCTGCCCCACCTCTCGCCGTACTCCGAGCGACTACGCCGCCTGAACCGCGTCAGCCTCCG
CCTCTTCGACGCGCTTCGTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MVQDPLGAFLPNAIMRRSPHRSGPLSGLSFAVKDLFDVAGDVTGCGNPDWAACHDTPERDAWAVDAMCCAGATLTGKTIT
DEISLGLLGINRFYGTPLNPRAPDRVPGGSSSGSASAVAGGLVDVALGTDSGGSVRTPASFCGIYGLRPTHGRISVAGLM
TQAPSFDTVGYFTRDALTFGRVGSVLLAEPIADGLQPDIVIASDCFALADEPVRAALQPVVARLRSVAPATEAALADGEL
LAWGRHQRVLQKSEFHATFRDWIDRVNPRFSSEVAGAFADDGRIAPQDLATAEVFRAAASKRLDDVLDGRRMLCLPTTPI
LPIARDAGLSEMRTAVHRIVDLTCIAGLTGLPQVNLPVATSGAVPVGLSLIGWRGGDASLLAAAQTLAREFSIGLFEEPS

Sequences:

>Translated_400_residues
MVQDPLGAFLPNAIMRRSPHRSGPLSGLSFAVKDLFDVAGDVTGCGNPDWAACHDTPERDAWAVDAMCCAGATLTGKTIT
DEISLGLLGINRFYGTPLNPRAPDRVPGGSSSGSASAVAGGLVDVALGTDSGGSVRTPASFCGIYGLRPTHGRISVAGLM
TQAPSFDTVGYFTRDALTFGRVGSVLLAEPIADGLQPDIVIASDCFALADEPVRAALQPVVARLRSVAPATEAALADGEL
LAWGRHQRVLQKSEFHATFRDWIDRVNPRFSSEVAGAFADDGRIAPQDLATAEVFRAAASKRLDDVLDGRRMLCLPTTPI
LPIARDAGLSEMRTAVHRIVDLTCIAGLTGLPQVNLPVATSGAVPVGLSLIGWRGGDASLLAAAQTLAREFSIGLFEEPS
>Mature_400_residues
MVQDPLGAFLPNAIMRRSPHRSGPLSGLSFAVKDLFDVAGDVTGCGNPDWAACHDTPERDAWAVDAMCCAGATLTGKTIT
DEISLGLLGINRFYGTPLNPRAPDRVPGGSSSGSASAVAGGLVDVALGTDSGGSVRTPASFCGIYGLRPTHGRISVAGLM
TQAPSFDTVGYFTRDALTFGRVGSVLLAEPIADGLQPDIVIASDCFALADEPVRAALQPVVARLRSVAPATEAALADGEL
LAWGRHQRVLQKSEFHATFRDWIDRVNPRFSSEVAGAFADDGRIAPQDLATAEVFRAAASKRLDDVLDGRRMLCLPTTPI
LPIARDAGLSEMRTAVHRIVDLTCIAGLTGLPQVNLPVATSGAVPVGLSLIGWRGGDASLLAAAQTLAREFSIGLFEEPS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=450, Percent_Identity=25.1111111111111, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=159, Percent_Identity=36.4779874213836, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=414, Percent_Identity=24.6376811594203, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=143, Percent_Identity=37.0629370629371, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=248, Percent_Identity=27.0161290322581, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=156, Percent_Identity=40.3846153846154, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=130, Percent_Identity=40.7692307692308, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648113, Length=197, Percent_Identity=36.0406091370558, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356731, Length=138, Percent_Identity=35.5072463768116, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 41782; Mature: 41782

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQDPLGAFLPNAIMRRSPHRSGPLSGLSFAVKDLFDVAGDVTGCGNPDWAACHDTPERD
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AWAVDAMCCAGATLTGKTITDEISLGLLGINRFYGTPLNPRAPDRVPGGSSSGSASAVAG
HHHHHHHHHCCCEECCCEEHHHHHHHEEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHC
GLVDVALGTDSGGSVRTPASFCGIYGLRPTHGRISVAGLMTQAPSFDTVGYFTRDALTFG
CEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHH
RVGSVLLAEPIADGLQPDIVIASDCFALADEPVRAALQPVVARLRSVAPATEAALADGEL
HHCHHHHHHHHHCCCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCE
LAWGRHQRVLQKSEFHATFRDWIDRVNPRFSSEVAGAFADDGRIAPQDLATAEVFRAAAS
EECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KRLDDVLDGRRMLCLPTTPILPIARDAGLSEMRTAVHRIVDLTCIAGLTGLPQVNLPVAT
HHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
SGAVPVGLSLIGWRGGDASLLAAAQTLAREFSIGLFEEPS
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVQDPLGAFLPNAIMRRSPHRSGPLSGLSFAVKDLFDVAGDVTGCGNPDWAACHDTPERD
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AWAVDAMCCAGATLTGKTITDEISLGLLGINRFYGTPLNPRAPDRVPGGSSSGSASAVAG
HHHHHHHHHCCCEECCCEEHHHHHHHEEEHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHC
GLVDVALGTDSGGSVRTPASFCGIYGLRPTHGRISVAGLMTQAPSFDTVGYFTRDALTFG
CEEEEEECCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHH
RVGSVLLAEPIADGLQPDIVIASDCFALADEPVRAALQPVVARLRSVAPATEAALADGEL
HHCHHHHHHHHHCCCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCE
LAWGRHQRVLQKSEFHATFRDWIDRVNPRFSSEVAGAFADDGRIAPQDLATAEVFRAAAS
EECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
KRLDDVLDGRRMLCLPTTPILPIARDAGLSEMRTAVHRIVDLTCIAGLTGLPQVNLPVAT
HHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEC
SGAVPVGLSLIGWRGGDASLLAAAQTLAREFSIGLFEEPS
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA