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The map label for this gene is livM [H]

Identifier: 146340683

GI number: 146340683

Start: 3896926

End: 3897894

Strand: Direct

Name: livM [H]

Synonym: BRADO3734

Alternate gene names: 146340683

Gene position: 3896926-3897894 (Clockwise)

Preceding gene: 146340682

Following gene: 146340684

Centisome position: 52.26

GC content: 62.75

Gene sequence:

>969_bases
ATGACATCGATCACCGACGAGACATTGCCGATCACCCCGCGCGCGATGCGCGACGAGATGATCGCGTTCGTCATCATGGC
GGCGCTGCTCGCCATCGTGCCGTTCACCGGCCTGTACCCGTTCTTCGTCATGCAGGCGCTGTGCTTCGCGCTGCTGGCCT
GCGCCTTCAACCTGCTGATCGGCTATGGCGGCCTGCTGTCGTTCGGCCACGCGATGTTCTTGGGAACGGCCGGCTATTGC
AGCGCGCATGCGCTGAAGGTCTGGGGTTTCCCGCCGGAGCTCGGCATCCTCACCGGCATCGCCGGCGCCTTCGTGCTGTC
GATCGTCACCGGCTACATTTCGATCCGCCGCCAGGGCATCTATTTCTCGATGATCACCCTGGCGCTGTCGCAGCTCCTCT
ATTTCGTCTATCTGCAGACGCCGTTCACCCATGGCGAGGATGGCATCCAGGGCATCCCGCAGGGCAAGCTGTTCGGCTTC
ATCGACCTGTCGAAGCCGTTCACGCTGTACTACGTCGTGCTGGTCGGGTTCCTCGCTGGCTTCTTGCTGATCTTCCGCAC
CATCAACTCGCCGTTCGGCGAGGTGCTGAAATCGATCCGCGAGAACGAGCAGCGCGCGATCTCGCTCGGCTACAAGACCG
ACCAGTACAAGCTGCTGGCCTTCATCCTGTCCGGTACCATCGCCGGTTTCGCCGGCTCGCTGAAGGTGTTCGTGGCCCAG
AACGCCTCGCTGACCGACGTGCACTGGTCGATGTCCGGCGAGATCGTGCTGATGACGCTGGTCGGCGGCCTCGGAACGGT
GTTCGGGCCCGTTGTCGGCGCCTTCGTGATTATCGCCATGCAGCAGTATCTGGCTGGGTTCGGGCAGTGGGTGACGGTGA
TTCAGGGCGTCATCTTCGTCGCTTGCGTGCTCACCTTCCGCCGCGGCGTGGTCGGGGAAGTCGCTCATCTGTTCAAGCGA
TCCCTCTAA

Upstream 100 bases:

>100_bases
AAATATTTCTATCCCGAGGCTTCCAACACCGTGGTCTTCGTTCTGATGGTCCTGGTGCTCCTGGTGAAGCCGACGGGTCT
GACCGGACGGGCGGCCTGAC

Downstream 100 bases:

>100_bases
GTCATTGATCTAGATGAAATTTGTCGGAGAGGCAGAAAGCGGTGGATGAGCCGGCCACCGCTTCTGCTGCGGCGTGATCC
GGAGTATGACAGTTCTGTGA

Product: ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTSITDETLPITPRAMRDEMIAFVIMAALLAIVPFTGLYPFFVMQALCFALLACAFNLLIGYGGLLSFGHAMFLGTAGYC
SAHALKVWGFPPELGILTGIAGAFVLSIVTGYISIRRQGIYFSMITLALSQLLYFVYLQTPFTHGEDGIQGIPQGKLFGF
IDLSKPFTLYYVVLVGFLAGFLLIFRTINSPFGEVLKSIRENEQRAISLGYKTDQYKLLAFILSGTIAGFAGSLKVFVAQ
NASLTDVHWSMSGEIVLMTLVGGLGTVFGPVVGAFVIIAMQQYLAGFGQWVTVIQGVIFVACVLTFRRGVVGEVAHLFKR
SL

Sequences:

>Translated_322_residues
MTSITDETLPITPRAMRDEMIAFVIMAALLAIVPFTGLYPFFVMQALCFALLACAFNLLIGYGGLLSFGHAMFLGTAGYC
SAHALKVWGFPPELGILTGIAGAFVLSIVTGYISIRRQGIYFSMITLALSQLLYFVYLQTPFTHGEDGIQGIPQGKLFGF
IDLSKPFTLYYVVLVGFLAGFLLIFRTINSPFGEVLKSIRENEQRAISLGYKTDQYKLLAFILSGTIAGFAGSLKVFVAQ
NASLTDVHWSMSGEIVLMTLVGGLGTVFGPVVGAFVIIAMQQYLAGFGQWVTVIQGVIFVACVLTFRRGVVGEVAHLFKR
SL
>Mature_321_residues
TSITDETLPITPRAMRDEMIAFVIMAALLAIVPFTGLYPFFVMQALCFALLACAFNLLIGYGGLLSFGHAMFLGTAGYCS
AHALKVWGFPPELGILTGIAGAFVLSIVTGYISIRRQGIYFSMITLALSQLLYFVYLQTPFTHGEDGIQGIPQGKLFGFI
DLSKPFTLYYVVLVGFLAGFLLIFRTINSPFGEVLKSIRENEQRAISLGYKTDQYKLLAFILSGTIAGFAGSLKVFVAQN
ASLTDVHWSMSGEIVLMTLVGGLGTVFGPVVGAFVIIAMQQYLAGFGQWVTVIQGVIFVACVLTFRRGVVGEVAHLFKRS
L

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=300, Percent_Identity=25.6666666666667, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34974; Mature: 34843

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSITDETLPITPRAMRDEMIAFVIMAALLAIVPFTGLYPFFVMQALCFALLACAFNLLI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GYGGLLSFGHAMFLGTAGYCSAHALKVWGFPPELGILTGIAGAFVLSIVTGYISIRRQGI
HHHHHHHHHHHHHHCCCCCHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
YFSMITLALSQLLYFVYLQTPFTHGEDGIQGIPQGKLFGFIDLSKPFTLYYVVLVGFLAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
FLLIFRTINSPFGEVLKSIRENEQRAISLGYKTDQYKLLAFILSGTIAGFAGSLKVFVAQ
HHHHHHHHCCCHHHHHHHHHHCHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
NASLTDVHWSMSGEIVLMTLVGGLGTVFGPVVGAFVIIAMQQYLAGFGQWVTVIQGVIFV
CCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACVLTFRRGVVGEVAHLFKRSL
HHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TSITDETLPITPRAMRDEMIAFVIMAALLAIVPFTGLYPFFVMQALCFALLACAFNLLI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
GYGGLLSFGHAMFLGTAGYCSAHALKVWGFPPELGILTGIAGAFVLSIVTGYISIRRQGI
HHHHHHHHHHHHHHCCCCCHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
YFSMITLALSQLLYFVYLQTPFTHGEDGIQGIPQGKLFGFIDLSKPFTLYYVVLVGFLAG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
FLLIFRTINSPFGEVLKSIRENEQRAISLGYKTDQYKLLAFILSGTIAGFAGSLKVFVAQ
HHHHHHHHCCCHHHHHHHHHHCHHHEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
NASLTDVHWSMSGEIVLMTLVGGLGTVFGPVVGAFVIIAMQQYLAGFGQWVTVIQGVIFV
CCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACVLTFRRGVVGEVAHLFKRSL
HHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]