Click here to switch to the map view.

The map label for this gene is dme [H]

Identifier: 146340340

GI number: 146340340

Start: 3561435

End: 3563747

Strand: Direct

Name: dme [H]

Synonym: BRADO3367

Alternate gene names: 146340340

Gene position: 3561435-3563747 (Clockwise)

Preceding gene: 146340333

Following gene: 146340341

Centisome position: 47.76

GC content: 66.97

Gene sequence:

>2313_bases
ATGTCGTCGTCCTATTCTGAAGATCTTCGTTCCGCAGCCCTGGCCTATCACCGCCTGCCGCGGCCCGGCAAGCTGGAGAT
CCAGGCCTCCAAGCCGCTCGCCAACCAGCGCGACCTCGCGCTTGCCTATTCCCCGGGCGTGGCCGCTGCGTGCACCGAGA
TCGCCAACAACCCGGCCGAGGCGGCCTCGCTGACGGCGCGCGCCAACCTCGTCGCCGTCGTCTCCAACGGTACGGCCGTG
CTTGGTCTCGGCAATATCGGGCCGCTCGCCTCCAAGCCGGTCATGGAGGGCAAGGCGGTCCTGTTCAAGAAGTTCGCCGG
CATCGACGTGTTCGACATCGAGATATCAGCCGACACCATCGACCGCGTGGTCGAAACGGTGGCGGCGCTGGAGCCGACCT
TCGGTGGCATCAACCTCGAGGACATCAAGGGGCCGGAGTGCTTCGAGATCGAGGCGCGGCTCAAGGAGCGGATGAAGATC
CCGGTCTTCCACGATGACCAGCACGGCACCGCGATCATTGTCGGCGCCGCGATCAAGAACGCGCTGTCGCTGGCCGGCAA
GCAGCTGTCCGAAGTCAAGATCGTGACCTCGGGCGCGGGAGCGGCCGCCATCGCCTGTCTCAATCTCTTGGTGTCGATGG
GCGCGCAACGCAAGAACATCTGGGTCTGCGACATCGACGGCGTGGTGCATGAGGGCCGCAACACGCTGATGGACCCGTGG
AAGGCGGTCTATGCGCAGAAGACCGACAAGCGGACCCTGGCCGAGGTGATCGGTGGCGCCGACATCTTCCTCGGCCTCTC
CGCCGGCGGCGTGCTGAAGCCGGAGCTGTTGAAGCAGATGGCCGAGAAGCCGCTGATCATGGCGCTCGCCAATCCCAACC
CGGAGATCATGCCGGAGGAGGCGCGCGCGGCGCGGCCCGATGCGATGATCTGCACCGGGCGTTCGGACTTCCCGAACCAG
GTCAACAACGTCCTGTGCTTTCCGTTCATCTTCCGGGGCGCGCTCGACGTCGGCGCCACCGCGATCAACGAGGCGATGAA
GCAGGCGGCGGTCGATGCGATCGCGCTGCTCGCGCAGGACCCGCCGTCGGACGCGGTGAACCGCGGTTTTGACACCGGCG
AGACGCAGGGCTTCGGCCCGGGCTCGCTGATCCCGTCGCCGTTCGATCCGCGCCTGATCCTGCGCATTGCGCCGGCGGTG
GCGAAGGCGGCGATGGAGTCGGGCGTCGCGACCCGGCCGATCACCAATTTCGACGAATACACTGCGCTGCTGGAGCGCTT
CGCGTTCCGCTCCGGCCTCATCATGAAGCCTGTGTTCGCCAAGGCGAAGGCGCAGCCGGTGCGCGTGATCTACGCCGAGG
GCGAAGACGAACGTGTGCTGCGCGCGACGCAGGTCGTCTTGGAAGAGAAGCTGGCGCGGCCGATCCTGGTCGGGCGTCCG
AGCGTGGTCGAGGCGCGCATCAAGCGCTTCGGCCTGTCGATCAAGGCGGGCCGTGATTTCGATCTCGTCAATCCCGAGGA
CGATCCGCGCTACCGCTCCTACGTACAGAGCTACATCGAGATCGCCGGCCGCCGCGGCGTGACGCCGGATGCGGCGCGCA
CGGTGGTGCGCACCAATGCCACCGTCATCGCGGCGCTCGCGGTGGTCCGCGGCGAGGCGGATGCGATGATCTGCGGCGTC
GAGGGGCGCTACATGAGCCATCTTCGTCACGTCCGCGAGATCATCGGCTTCATGCCGGGGGTCAGCGACTACGCCGCGCT
GGCGCTCACCATCACCAGCAAGGGTGCCTATTTCATCGGCGACACGCAGGTGCGCCCCGATCCGACCGCCGAGGAGCTCG
CCGAGATGGCCTCGCTGGCGGCCAATCACGTCACACGCTTCAATATCAAGCCGAAGATCGCATTCGTGTCGCATTCCGAT
TTCGGCAGCTACGACACGGACTCCTCGCGCAAGATGCGCAGGGCCACCGCGCTGTTGAAGCAGCATCGTCCGGACCTCGA
GGCCGACGGCGAGATGCAGGGCGACACGGCGCTGTCGGCGACCGCGCGCAAGGCGATCCTGCCGCACTCGGATCTCGACG
GCTCGGCCAACGTGCTGATCATGCCGAATCTCGACGCCGCCAACGTCGCCTATCAGATGATCAAGGTGCTGGCGGATGCG
GTTCCCGTCGGCCCGATCCTGATCGGCCCGGCACGCCCGGCGCACATCTTGACCCCGTCGGTGACCGCGCGCGGCATTCT
CAACATGACGGCGGTCGCTGCCGTCGAGGCGCAGGAACGGGCGGGGAGGGCGCAGCCGACGCTGTTCGCGTGA

Upstream 100 bases:

>100_bases
CGCCCAACGTGCATCACATGCGCTTCCACCGGCCGGAAAAATCGACGTCTGACCGAACCTCAAGAGACCAAAAATCCGGG
TCGGAAACGCTGGAGCACGT

Downstream 100 bases:

>100_bases
TCGTATTGACGTGATCGAAGTGTGGCGGAATTCGTCCGGTTTCGGTCGCATTCCGCCCGCCTTTATTGTTGAAAAGCGTT
CGCGATGTCACACATCCCGG

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 770; Mature: 769

Protein sequence:

>770_residues
MSSSYSEDLRSAALAYHRLPRPGKLEIQASKPLANQRDLALAYSPGVAAACTEIANNPAEAASLTARANLVAVVSNGTAV
LGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEISADTIDRVVETVAALEPTFGGINLEDIKGPECFEIEARLKERMKI
PVFHDDQHGTAIIVGAAIKNALSLAGKQLSEVKIVTSGAGAAAIACLNLLVSMGAQRKNIWVCDIDGVVHEGRNTLMDPW
KAVYAQKTDKRTLAEVIGGADIFLGLSAGGVLKPELLKQMAEKPLIMALANPNPEIMPEEARAARPDAMICTGRSDFPNQ
VNNVLCFPFIFRGALDVGATAINEAMKQAAVDAIALLAQDPPSDAVNRGFDTGETQGFGPGSLIPSPFDPRLILRIAPAV
AKAAMESGVATRPITNFDEYTALLERFAFRSGLIMKPVFAKAKAQPVRVIYAEGEDERVLRATQVVLEEKLARPILVGRP
SVVEARIKRFGLSIKAGRDFDLVNPEDDPRYRSYVQSYIEIAGRRGVTPDAARTVVRTNATVIAALAVVRGEADAMICGV
EGRYMSHLRHVREIIGFMPGVSDYAALALTITSKGAYFIGDTQVRPDPTAEELAEMASLAANHVTRFNIKPKIAFVSHSD
FGSYDTDSSRKMRRATALLKQHRPDLEADGEMQGDTALSATARKAILPHSDLDGSANVLIMPNLDAANVAYQMIKVLADA
VPVGPILIGPARPAHILTPSVTARGILNMTAVAAVEAQERAGRAQPTLFA

Sequences:

>Translated_770_residues
MSSSYSEDLRSAALAYHRLPRPGKLEIQASKPLANQRDLALAYSPGVAAACTEIANNPAEAASLTARANLVAVVSNGTAV
LGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEISADTIDRVVETVAALEPTFGGINLEDIKGPECFEIEARLKERMKI
PVFHDDQHGTAIIVGAAIKNALSLAGKQLSEVKIVTSGAGAAAIACLNLLVSMGAQRKNIWVCDIDGVVHEGRNTLMDPW
KAVYAQKTDKRTLAEVIGGADIFLGLSAGGVLKPELLKQMAEKPLIMALANPNPEIMPEEARAARPDAMICTGRSDFPNQ
VNNVLCFPFIFRGALDVGATAINEAMKQAAVDAIALLAQDPPSDAVNRGFDTGETQGFGPGSLIPSPFDPRLILRIAPAV
AKAAMESGVATRPITNFDEYTALLERFAFRSGLIMKPVFAKAKAQPVRVIYAEGEDERVLRATQVVLEEKLARPILVGRP
SVVEARIKRFGLSIKAGRDFDLVNPEDDPRYRSYVQSYIEIAGRRGVTPDAARTVVRTNATVIAALAVVRGEADAMICGV
EGRYMSHLRHVREIIGFMPGVSDYAALALTITSKGAYFIGDTQVRPDPTAEELAEMASLAANHVTRFNIKPKIAFVSHSD
FGSYDTDSSRKMRRATALLKQHRPDLEADGEMQGDTALSATARKAILPHSDLDGSANVLIMPNLDAANVAYQMIKVLADA
VPVGPILIGPARPAHILTPSVTARGILNMTAVAAVEAQERAGRAQPTLFA
>Mature_769_residues
SSSYSEDLRSAALAYHRLPRPGKLEIQASKPLANQRDLALAYSPGVAAACTEIANNPAEAASLTARANLVAVVSNGTAVL
GLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEISADTIDRVVETVAALEPTFGGINLEDIKGPECFEIEARLKERMKIP
VFHDDQHGTAIIVGAAIKNALSLAGKQLSEVKIVTSGAGAAAIACLNLLVSMGAQRKNIWVCDIDGVVHEGRNTLMDPWK
AVYAQKTDKRTLAEVIGGADIFLGLSAGGVLKPELLKQMAEKPLIMALANPNPEIMPEEARAARPDAMICTGRSDFPNQV
NNVLCFPFIFRGALDVGATAINEAMKQAAVDAIALLAQDPPSDAVNRGFDTGETQGFGPGSLIPSPFDPRLILRIAPAVA
KAAMESGVATRPITNFDEYTALLERFAFRSGLIMKPVFAKAKAQPVRVIYAEGEDERVLRATQVVLEEKLARPILVGRPS
VVEARIKRFGLSIKAGRDFDLVNPEDDPRYRSYVQSYIEIAGRRGVTPDAARTVVRTNATVIAALAVVRGEADAMICGVE
GRYMSHLRHVREIIGFMPGVSDYAALALTITSKGAYFIGDTQVRPDPTAEELAEMASLAANHVTRFNIKPKIAFVSHSDF
GSYDTDSSRKMRRATALLKQHRPDLEADGEMQGDTALSATARKAILPHSDLDGSANVLIMPNLDAANVAYQMIKVLADAV
PVGPILIGPARPAHILTPSVTARGILNMTAVAAVEAQERAGRAQPTLFA

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=401, Percent_Identity=23.1920199501247, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI4505145, Length=368, Percent_Identity=25.8152173913043, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI270265879, Length=368, Percent_Identity=25.8152173913043, Blast_Score=72, Evalue=3e-12,
Organism=Escherichia coli, GI1788806, Length=756, Percent_Identity=57.8042328042328, Blast_Score=873, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=313, Percent_Identity=29.7124600638978, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1788800, Length=326, Percent_Identity=31.9018404907975, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081919, Length=296, Percent_Identity=28.3783783783784, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322823, Length=234, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356279, Length=400, Percent_Identity=27.25, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281362672, Length=400, Percent_Identity=27.25, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281362674, Length=400, Percent_Identity=27.25, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24646388, Length=403, Percent_Identity=27.2952853598015, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24646386, Length=403, Percent_Identity=27.2952853598015, Blast_Score=73, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 82213; Mature: 82081

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSYSEDLRSAALAYHRLPRPGKLEIQASKPLANQRDLALAYSPGVAAACTEIANNPAE
CCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCHH
AASLTARANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEISADTI
HHHHEECCEEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHH
DRVVETVAALEPTFGGINLEDIKGPECFEIEARLKERMKIPVFHDDQHGTAIIVGAAIKN
HHHHHHHHHHCCCCCCCCHHHCCCCCEEEHHHHHHHCCCCCEEECCCCCCEEEEHHHHHH
ALSLAGKQLSEVKIVTSGAGAAAIACLNLLVSMGAQRKNIWVCDIDGVVHEGRNTLMDPW
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCCHHHHCHH
KAVYAQKTDKRTLAEVIGGADIFLGLSAGGVLKPELLKQMAEKPLIMALANPNPEIMPEE
HHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCH
ARAARPDAMICTGRSDFPNQVNNVLCFPFIFRGALDVGATAINEAMKQAAVDAIALLAQD
HHCCCCCCEEEECCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPSDAVNRGFDTGETQGFGPGSLIPSPFDPRLILRIAPAVAKAAMESGVATRPITNFDEY
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHH
TALLERFAFRSGLIMKPVFAKAKAQPVRVIYAEGEDERVLRATQVVLEEKLARPILVGRP
HHHHHHHHHHCCCEECHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCC
SVVEARIKRFGLSIKAGRDFDLVNPEDDPRYRSYVQSYIEIAGRRGVTPDAARTVVRTNA
HHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCH
TVIAALAVVRGEADAMICGVEGRYMSHLRHVREIIGFMPGVSDYAALALTITSKGAYFIG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEE
DTQVRPDPTAEELAEMASLAANHVTRFNIKPKIAFVSHSDFGSYDTDSSRKMRRATALLK
CCCCCCCCCHHHHHHHHHHHHHCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
QHRPDLEADGEMQGDTALSATARKAILPHSDLDGSANVLIMPNLDAANVAYQMIKVLADA
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
VPVGPILIGPARPAHILTPSVTARGILNMTAVAAVEAQERAGRAQPTLFA
CCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSSYSEDLRSAALAYHRLPRPGKLEIQASKPLANQRDLALAYSPGVAAACTEIANNPAE
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCHH
AASLTARANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEISADTI
HHHHEECCEEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHH
DRVVETVAALEPTFGGINLEDIKGPECFEIEARLKERMKIPVFHDDQHGTAIIVGAAIKN
HHHHHHHHHHCCCCCCCCHHHCCCCCEEEHHHHHHHCCCCCEEECCCCCCEEEEHHHHHH
ALSLAGKQLSEVKIVTSGAGAAAIACLNLLVSMGAQRKNIWVCDIDGVVHEGRNTLMDPW
HHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHCCCHHHHCHH
KAVYAQKTDKRTLAEVIGGADIFLGLSAGGVLKPELLKQMAEKPLIMALANPNPEIMPEE
HHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCH
ARAARPDAMICTGRSDFPNQVNNVLCFPFIFRGALDVGATAINEAMKQAAVDAIALLAQD
HHCCCCCCEEEECCCCCCHHHHCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PPSDAVNRGFDTGETQGFGPGSLIPSPFDPRLILRIAPAVAKAAMESGVATRPITNFDEY
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHH
TALLERFAFRSGLIMKPVFAKAKAQPVRVIYAEGEDERVLRATQVVLEEKLARPILVGRP
HHHHHHHHHHCCCEECHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCC
SVVEARIKRFGLSIKAGRDFDLVNPEDDPRYRSYVQSYIEIAGRRGVTPDAARTVVRTNA
HHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCH
TVIAALAVVRGEADAMICGVEGRYMSHLRHVREIIGFMPGVSDYAALALTITSKGAYFIG
HHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEE
DTQVRPDPTAEELAEMASLAANHVTRFNIKPKIAFVSHSDFGSYDTDSSRKMRRATALLK
CCCCCCCCCHHHHHHHHHHHHHCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
QHRPDLEADGEMQGDTALSATARKAILPHSDLDGSANVLIMPNLDAANVAYQMIKVLADA
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
VPVGPILIGPARPAHILTPSVTARGILNMTAVAAVEAQERAGRAQPTLFA
CCCCCEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]