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The map label for this gene is purN [H]

Identifier: 146340322

GI number: 146340322

Start: 3542140

End: 3542793

Strand: Direct

Name: purN [H]

Synonym: BRADO3345

Alternate gene names: 146340322

Gene position: 3542140-3542793 (Clockwise)

Preceding gene: 146340321

Following gene: 146340325

Centisome position: 47.5

GC content: 67.74

Gene sequence:

>654_bases
ATGAAGCGCCGCGTCGCGATCCTGATTTCCGGCCGCGGGTCCAACATGGCCGCACTCATCCGCGCGGCGGCCGCGCCGGA
TTTCCCGGCCGAGATCGCGGTGGTGATCTCCAACCGTGCCGATGCCGCGGGCCTGCAGAAGGCTGCGGAGAGCGGCATCG
CCGTCCAGGTGATCGAGAGCAAACCGTTCGGCAAGGACCGCGCCGGTTTTGAGGCCAAGCTGCAGGCCGCGCTCGATGCG
CGCGGCGTCGAGCTGATCTGTCTCGCCGGCTTCATGCGGCTGTTCACCGCCGACTTCGTGCAGCGCTGGTACGGCCGGAT
GCTGAACATCCATCCCTCGCTGCTCCCATCGTTCCCCGGCCTCGATCCGCACGGCCAGGCGCTGCGCGCCGGCGTGAAGC
TCTCCGGCGCGACAGTCCATTTCGTGATTCCGGAGACCGATGCCGGCCCGATCGTGATGCAGGGCGCCGTGGTGGTGCGT
GACGACGACACGCCCGACACGCTGTCGGAGCGCATCCTCGGCGTCGAGCATCGCATCTATCCGGAAGCGCTGAAGCTGCT
GGCGAGAGATCTCGTGCGCCTCGAAGGCGATCTGTGCCGCACGTCGGCGACGGAGCAGGCGGATCAGGTGTTGATCTGGC
CGATGGTCTCGTAG

Upstream 100 bases:

>100_bases
GAGCATGTCGCGCGGCTCGGCGAGGTCGTCGCAGCCACGGGCGAGGAGCGCGTGATCTACACCGGCGCGCTCGATTTGTC
GCTGTGAGACGAGCCCGCCG

Downstream 100 bases:

>100_bases
TTCGATTGCGGGTCCGTGGCGCGGTGACGGAGTTTCTCTGCGGAGACAGTACGCTCTACGGAGAGAACCCCTCACCCAAG
CGAGTATGACGCTGCCGCCG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKRRVAILISGRGSNMAALIRAAAAPDFPAEIAVVISNRADAAGLQKAAESGIAVQVIESKPFGKDRAGFEAKLQAALDA
RGVELICLAGFMRLFTADFVQRWYGRMLNIHPSLLPSFPGLDPHGQALRAGVKLSGATVHFVIPETDAGPIVMQGAVVVR
DDDTPDTLSERILGVEHRIYPEALKLLARDLVRLEGDLCRTSATEQADQVLIWPMVS

Sequences:

>Translated_217_residues
MKRRVAILISGRGSNMAALIRAAAAPDFPAEIAVVISNRADAAGLQKAAESGIAVQVIESKPFGKDRAGFEAKLQAALDA
RGVELICLAGFMRLFTADFVQRWYGRMLNIHPSLLPSFPGLDPHGQALRAGVKLSGATVHFVIPETDAGPIVMQGAVVVR
DDDTPDTLSERILGVEHRIYPEALKLLARDLVRLEGDLCRTSATEQADQVLIWPMVS
>Mature_217_residues
MKRRVAILISGRGSNMAALIRAAAAPDFPAEIAVVISNRADAAGLQKAAESGIAVQVIESKPFGKDRAGFEAKLQAALDA
RGVELICLAGFMRLFTADFVQRWYGRMLNIHPSLLPSFPGLDPHGQALRAGVKLSGATVHFVIPETDAGPIVMQGAVVVR
DDDTPDTLSERILGVEHRIYPEALKLLARDLVRLEGDLCRTSATEQADQVLIWPMVS

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=200, Percent_Identity=50, Blast_Score=197, Evalue=7e-51,
Organism=Homo sapiens, GI209869995, Length=200, Percent_Identity=50, Blast_Score=197, Evalue=7e-51,
Organism=Homo sapiens, GI209869993, Length=200, Percent_Identity=50, Blast_Score=197, Evalue=7e-51,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=38.5416666666667, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1787483, Length=189, Percent_Identity=32.8042328042328, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=181, Percent_Identity=41.9889502762431, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=28.1407035175879, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582400, Length=188, Percent_Identity=49.468085106383, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23363; Mature: 23363

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRVAILISGRGSNMAALIRAAAAPDFPAEIAVVISNRADAAGLQKAAESGIAVQVIES
CCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
KPFGKDRAGFEAKLQAALDARGVELICLAGFMRLFTADFVQRWYGRMLNIHPSLLPSFPG
CCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
LDPHGQALRAGVKLSGATVHFVIPETDAGPIVMQGAVVVRDDDTPDTLSERILGVEHRIY
CCCCCHHHHCCEEECCCEEEEEECCCCCCCEEEECEEEEECCCCHHHHHHHHHCCHHHCC
PEALKLLARDLVRLEGDLCRTSATEQADQVLIWPMVS
HHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKRRVAILISGRGSNMAALIRAAAAPDFPAEIAVVISNRADAAGLQKAAESGIAVQVIES
CCCEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
KPFGKDRAGFEAKLQAALDARGVELICLAGFMRLFTADFVQRWYGRMLNIHPSLLPSFPG
CCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCC
LDPHGQALRAGVKLSGATVHFVIPETDAGPIVMQGAVVVRDDDTPDTLSERILGVEHRIY
CCCCCHHHHCCEEECCCEEEEEECCCCCCCEEEECEEEEECCCCHHHHHHHHHCCHHHCC
PEALKLLARDLVRLEGDLCRTSATEQADQVLIWPMVS
HHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]