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The map label for this gene is yogA [H]

Identifier: 146339815

GI number: 146339815

Start: 3017903

End: 3018916

Strand: Direct

Name: yogA [H]

Synonym: BRADO2816

Alternate gene names: 146339815

Gene position: 3017903-3018916 (Clockwise)

Preceding gene: 146339814

Following gene: 146339823

Centisome position: 40.47

GC content: 69.13

Gene sequence:

>1014_bases
ATGAAATGCTATGAGCTGCAAGGACCGGGCGGAATCGATGCGCTGGCGCTGGTCGACAAACCCGTGCCGACGCCCGCGCG
CGGCGAGGTGCTGGTGCGGATCGCCGCCGTCAGCCTCAATTATCGCGATCTGCTCACGGTAAAGGGCGGCTACGGCTCCA
GGCAGAAATTTCCGCTGGTGCCGCTGTCGGACGGCGCCGGGGTCGTCGAGGCGGTCGGCGAGGGCGTATCCACATTTGCG
CCCGGTGACCGGGTGATCGGCAGCTTCTTCGAGAGCTGGACAGGCGGCGAGCCGAGCGAGACCAAGATGCGCGGCGCGCT
CGGCGGCGCGGTCGACGGCACGTTGAGCGAATATCGCATCTTTCCCGCGCAGGCGCTGGTGAGAACGCCGGCGCATCTCA
CCGACATCGAGGCGGCGACCTTGCCGTGCGCCGGCGTCACCGCCTGGAGCGCGGTGGTCAAGCTCGGCGGCATCCGGCCG
GGCCAGACCGTGCTGACCCAGGGCACCGGCGGCGTGTCGATCTTCGCACTGCAGATCGCGAAACTGGCCGGCGCGCGGGT
GATCGCGACGTCGTCGAGCGAGGCGAAGATCGCGCGGCTGACGGAGCTCGGCGCCGAGGTGACACTGAACTACAAGACCA
CGCCGGACTGGGGCAAGCACGCACGCGAGATCACCCGGCACGGCGTCGATCTCGTGGTCGAGGTCGGCGGCGTCGGCACG
CTCAACGAGTCGATCCGCGCCACCAGAATCGGCGGCATCATCGCCTTCATCGGCGTCCTCGCCGGCGCGCCGGCATCCGA
GCTGCGCCTGCCGCTGATGGTGATGCAGCAGCAGCGGCTGCAGGGCGTCACGGTCGGCTCCGTCGACGACCTGCAGGCGC
TCGCCAATGCGCTGGCGCTGCACCAGGTCAAGCCGGTGGTCGACCGCGTGTTCGCCTTCGCCGAGGCCCGCGAGGCGTTC
CATTACATGTCGAGCGGCGCGCATGTCGGCAAGGTCGCGATCGCGTTTGGCTGA

Upstream 100 bases:

>100_bases
GTCGCGGAACGAGCGGCTGCTTGATCGCCGGCGCGAAACGTCTCACCATCGCGCAAACGCCGCGCGGGAGTGACGCGCGT
GCAGGGGCGGGAGACATCCG

Downstream 100 bases:

>100_bases
CGGTTGCAGCCGTCTCTACCGCACACCACACACGCGTTGCCGCAGTCCGCTCTTGCCGCCGTCATTCCGGGCGCGCGCCA
GCGCGAGCCCGGAATCCATA

Product: putative alcohol dehydrogenase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKCYELQGPGGIDALALVDKPVPTPARGEVLVRIAAVSLNYRDLLTVKGGYGSRQKFPLVPLSDGAGVVEAVGEGVSTFA
PGDRVIGSFFESWTGGEPSETKMRGALGGAVDGTLSEYRIFPAQALVRTPAHLTDIEAATLPCAGVTAWSAVVKLGGIRP
GQTVLTQGTGGVSIFALQIAKLAGARVIATSSSEAKIARLTELGAEVTLNYKTTPDWGKHAREITRHGVDLVVEVGGVGT
LNESIRATRIGGIIAFIGVLAGAPASELRLPLMVMQQQRLQGVTVGSVDDLQALANALALHQVKPVVDRVFAFAEAREAF
HYMSSGAHVGKVAIAFG

Sequences:

>Translated_337_residues
MKCYELQGPGGIDALALVDKPVPTPARGEVLVRIAAVSLNYRDLLTVKGGYGSRQKFPLVPLSDGAGVVEAVGEGVSTFA
PGDRVIGSFFESWTGGEPSETKMRGALGGAVDGTLSEYRIFPAQALVRTPAHLTDIEAATLPCAGVTAWSAVVKLGGIRP
GQTVLTQGTGGVSIFALQIAKLAGARVIATSSSEAKIARLTELGAEVTLNYKTTPDWGKHAREITRHGVDLVVEVGGVGT
LNESIRATRIGGIIAFIGVLAGAPASELRLPLMVMQQQRLQGVTVGSVDDLQALANALALHQVKPVVDRVFAFAEAREAF
HYMSSGAHVGKVAIAFG
>Mature_337_residues
MKCYELQGPGGIDALALVDKPVPTPARGEVLVRIAAVSLNYRDLLTVKGGYGSRQKFPLVPLSDGAGVVEAVGEGVSTFA
PGDRVIGSFFESWTGGEPSETKMRGALGGAVDGTLSEYRIFPAQALVRTPAHLTDIEAATLPCAGVTAWSAVVKLGGIRP
GQTVLTQGTGGVSIFALQIAKLAGARVIATSSSEAKIARLTELGAEVTLNYKTTPDWGKHAREITRHGVDLVVEVGGVGT
LNESIRATRIGGIIAFIGVLAGAPASELRLPLMVMQQQRLQGVTVGSVDDLQALANALALHQVKPVVDRVFAFAEAREAF
HYMSSGAHVGKVAIAFG

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=349, Percent_Identity=25.7879656160458, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI22538444, Length=349, Percent_Identity=25.7879656160458, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI18379349, Length=363, Percent_Identity=28.3746556473829, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI24308257, Length=364, Percent_Identity=23.3516483516484, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI194239674, Length=335, Percent_Identity=25.0746268656716, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI13236495, Length=335, Percent_Identity=25.0746268656716, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI47519420, Length=359, Percent_Identity=27.8551532033426, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI28557745, Length=245, Percent_Identity=32.2448979591837, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI194239676, Length=250, Percent_Identity=28, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI156627571, Length=350, Percent_Identity=27.1428571428571, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87081918, Length=326, Percent_Identity=30.6748466257669, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI87082125, Length=346, Percent_Identity=26.3005780346821, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787863, Length=224, Percent_Identity=27.6785714285714, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786518, Length=323, Percent_Identity=25.077399380805, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71988145, Length=326, Percent_Identity=25.1533742331288, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562584, Length=327, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507255, Length=309, Percent_Identity=25.2427184466019, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562582, Length=283, Percent_Identity=27.9151943462898, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=277, Percent_Identity=26.7148014440433, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=340, Percent_Identity=26.7647058823529, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=325, Percent_Identity=27.0769230769231, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324486, Length=329, Percent_Identity=26.4437689969605, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=343, Percent_Identity=24.4897959183673, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319955, Length=345, Percent_Identity=26.9565217391304, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322619, Length=345, Percent_Identity=27.2463768115942, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319520, Length=265, Percent_Identity=27.1698113207547, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581345, Length=223, Percent_Identity=28.2511210762332, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221330659, Length=221, Percent_Identity=28.5067873303167, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19920632, Length=221, Percent_Identity=28.5067873303167, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581341, Length=381, Percent_Identity=26.246719160105, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581339, Length=381, Percent_Identity=25.9842519685039, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1

Molecular weight: Translated: 35076; Mature: 35076

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCYELQGPGGIDALALVDKPVPTPARGEVLVRIAAVSLNYRDLLTVKGGYGSRQKFPLV
CCEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCEE
PLSDGAGVVEAVGEGVSTFAPGDRVIGSFFESWTGGEPSETKMRGALGGAVDGTLSEYRI
ECCCCCHHHHHHCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHEEE
FPAQALVRTPAHLTDIEAATLPCAGVTAWSAVVKLGGIRPGQTVLTQGTGGVSIFALQIA
CHHHHHHHCCCHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
KLAGARVIATSSSEAKIARLTELGAEVTLNYKTTPDWGKHAREITRHGVDLVVEVGGVGT
HHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
LNESIRATRIGGIIAFIGVLAGAPASELRLPLMVMQQQRLQGVTVGSVDDLQALANALAL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HQVKPVVDRVFAFAEAREAFHYMSSGAHVGKVAIAFG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MKCYELQGPGGIDALALVDKPVPTPARGEVLVRIAAVSLNYRDLLTVKGGYGSRQKFPLV
CCEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCEE
PLSDGAGVVEAVGEGVSTFAPGDRVIGSFFESWTGGEPSETKMRGALGGAVDGTLSEYRI
ECCCCCHHHHHHCCCCHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHEEE
FPAQALVRTPAHLTDIEAATLPCAGVTAWSAVVKLGGIRPGQTVLTQGTGGVSIFALQIA
CHHHHHHHCCCHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHH
KLAGARVIATSSSEAKIARLTELGAEVTLNYKTTPDWGKHAREITRHGVDLVVEVGGVGT
HHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCC
LNESIRATRIGGIIAFIGVLAGAPASELRLPLMVMQQQRLQGVTVGSVDDLQALANALAL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
HQVKPVVDRVFAFAEAREAFHYMSSGAHVGKVAIAFG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.005 {cyclohexanol}} 0.004 {

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]