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The map label for this gene is irlS [H]

Identifier: 146339744

GI number: 146339744

Start: 2948673

End: 2950133

Strand: Direct

Name: irlS [H]

Synonym: BRADO2743

Alternate gene names: 146339744

Gene position: 2948673-2950133 (Clockwise)

Preceding gene: 146339743

Following gene: 146339745

Centisome position: 39.54

GC content: 68.04

Gene sequence:

>1461_bases
GTGACGGCCTTCGGTAAGCTGATCCGCACCACGGCGTTCCGGCTGACGCTGGTCTATCTGCTGTTGTTTGCGCTGTTTGC
CGCTTCGCTGCTCGGCTACTTCGCCTGGAACACCCGTCGCCTGATCACCGAGCAGATCTCGACCACGGTCAGCTCGGAGA
TCGGCGAGATGACCGACATCTACACCCGCGGCGGCCTGCGGTGGATCGTCGGCACGATCCAGAGCCGCGCGCTGCGCCCC
GGCGCCAACCTCTATCTGCTCACGACTCCGACCGGCCAACAGGTCGCCGGCAATGTCGATGCGCTGGCGCCCGGCGTGAT
GGGCAGCTCCGGCTGGTCGGAGACGTTCTACCGCCGGCTCGACGATTCCAGCGAGCAGAACCACCGCGCGCTGGTCTATG
TCACGCAGCTGTCGAGCGGCTTTCGGCTCCTGATCGGCCGCGACCTCGAGGAGCGGCGGCGGCTGTTCGGCATCGTCGCC
AAGGCCGCGCAATGGTCGGTGCTGGTCGTCATCGTGCTCGGCATCGGCGGCGGCATCTTCGTCGCGCGGCGCGTGCTGTA
CCGGATCGATGCGATGACCGGCACGACGCAGCGGATCATGGCGGGTGATCTCTCGGGACGACTGCCGGTCGGACGCAGCG
GCGACGAGCTCGATCGGCTCGCGGAAAACCTCAATGCGATGCTGGAGCGCATCGAGGCACTGATGACCGGGCTCAAGGAG
GTCTCCGACAACATCGCGCACGACCTCAAGACGCCGCTGACGCGGCTGCGCAACCGCGCCGAGGAGGCGCTGGCGAAATC
GCGCTCCGAGGACGAGTATCGCGCGGCGCTGGAGCGGACGATCGAGGAATCCGATGGCCTGATCCGCACCTTCAATGCGC
TCTTGATGATCGCGCGGGCGGAATCGGGACAGGCGCGCGGCGACATGGTCGAGTTCGATGCGGCCGACGTCGCCGGCGGC
ATTCACGAGCTGTATGAGCCGCTCGCCGAGGACAATGAGATGACCTTGCGGGTGAAGGCGTCGGCGGCGCCGGTGCATGG
CAATCGCGAGCTGATCAGCCAGGCGCTCGCCAATCTCGTCGAAAACGCGATCAAATACGGCAAGCCGGCCGCACTGCCGC
TCGATCCGGCCGCCGCAGCGGGGAGCCGCGAGATCCTGATCGAGGCGCGGCGCGAGGGCGGCCAGGTGCTGCTCAGCGTC
ACCGATCATGGCGAGGGCATTCCCGAAGCGGAACGCAAGCACGCCGTGGAACGGTTCGTGCGATTGGAGGCGAGCCGCAC
CTTGCCGGGTTCCGGCCTCGGCCTCAGTCTCGCCTCCGCCGTGGCGACCTTGCATGGCGGCGAGCTCAGGCTCGGCGACG
CCAGGCCGGGGCTGATCGCCACGCTCGTGCTGCCCGCCGGCGCAGGCCAGCCCGCGAAGCTTGCGGCGCCGACACAGGAT
GTGCCACAGAAGGCCGCATGA

Upstream 100 bases:

>100_bases
AACGTAATCGACGTCCACATCTCGCGGCTGCGCTCCAAGATCGACAAGGGGTTTGACCGCCCGCTGCTCCACACCATCCG
CGGCGCGGGGTACATGATCC

Downstream 100 bases:

>100_bases
CCTCCTCCGCGCCGGGAAACGCGGACGGGCAGAGCCGTCTGGCCGCGCGTTTCGTTGCCGGCCCGCATGTCCGTGCGCCC
GACACAGCCGAACACCGCCT

Product: putative two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTAFGKLIRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITEQISTTVSSEIGEMTDIYTRGGLRWIVGTIQSRALRP
GANLYLLTTPTGQQVAGNVDALAPGVMGSSGWSETFYRRLDDSSEQNHRALVYVTQLSSGFRLLIGRDLEERRRLFGIVA
KAAQWSVLVVIVLGIGGGIFVARRVLYRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMTGLKE
VSDNIAHDLKTPLTRLRNRAEEALAKSRSEDEYRAALERTIEESDGLIRTFNALLMIARAESGQARGDMVEFDAADVAGG
IHELYEPLAEDNEMTLRVKASAAPVHGNRELISQALANLVENAIKYGKPAALPLDPAAAAGSREILIEARREGGQVLLSV
TDHGEGIPEAERKHAVERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLGDARPGLIATLVLPAGAGQPAKLAAPTQD
VPQKAA

Sequences:

>Translated_486_residues
MTAFGKLIRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITEQISTTVSSEIGEMTDIYTRGGLRWIVGTIQSRALRP
GANLYLLTTPTGQQVAGNVDALAPGVMGSSGWSETFYRRLDDSSEQNHRALVYVTQLSSGFRLLIGRDLEERRRLFGIVA
KAAQWSVLVVIVLGIGGGIFVARRVLYRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMTGLKE
VSDNIAHDLKTPLTRLRNRAEEALAKSRSEDEYRAALERTIEESDGLIRTFNALLMIARAESGQARGDMVEFDAADVAGG
IHELYEPLAEDNEMTLRVKASAAPVHGNRELISQALANLVENAIKYGKPAALPLDPAAAAGSREILIEARREGGQVLLSV
TDHGEGIPEAERKHAVERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLGDARPGLIATLVLPAGAGQPAKLAAPTQD
VPQKAA
>Mature_485_residues
TAFGKLIRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITEQISTTVSSEIGEMTDIYTRGGLRWIVGTIQSRALRPG
ANLYLLTTPTGQQVAGNVDALAPGVMGSSGWSETFYRRLDDSSEQNHRALVYVTQLSSGFRLLIGRDLEERRRLFGIVAK
AAQWSVLVVIVLGIGGGIFVARRVLYRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMTGLKEV
SDNIAHDLKTPLTRLRNRAEEALAKSRSEDEYRAALERTIEESDGLIRTFNALLMIARAESGQARGDMVEFDAADVAGGI
HELYEPLAEDNEMTLRVKASAAPVHGNRELISQALANLVENAIKYGKPAALPLDPAAAAGSREILIEARREGGQVLLSVT
DHGEGIPEAERKHAVERFVRLEASRTLPGSGLGLSLASAVATLHGGELRLGDARPGLIATLVLPAGAGQPAKLAAPTQDV
PQKAA

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=335, Percent_Identity=28.6567164179104, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1788279, Length=286, Percent_Identity=27.972027972028, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787894, Length=297, Percent_Identity=28.2828282828283, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786912, Length=213, Percent_Identity=29.5774647887324, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789808, Length=247, Percent_Identity=28.7449392712551, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790861, Length=247, Percent_Identity=29.5546558704453, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=25.4612546125461, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789403, Length=341, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790551, Length=294, Percent_Identity=24.8299319727891, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=25, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52455; Mature: 52324

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFGKLIRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITEQISTTVSSEIGEMTDI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
YTRGGLRWIVGTIQSRALRPGANLYLLTTPTGQQVAGNVDALAPGVMGSSGWSETFYRRL
HHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHCCCCHHHCCCCCCCCCHHHHHHHHH
DDSSEQNHRALVYVTQLSSGFRLLIGRDLEERRRLFGIVAKAAQWSVLVVIVLGIGGGIF
CCCCCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VARRVLYRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMTGLKE
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSDNIAHDLKTPLTRLRNRAEEALAKSRSEDEYRAALERTIEESDGLIRTFNALLMIARA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
ESGQARGDMVEFDAADVAGGIHELYEPLAEDNEMTLRVKASAAPVHGNRELISQALANLV
CCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
ENAIKYGKPAALPLDPAAAAGSREILIEARREGGQVLLSVTDHGEGIPEAERKHAVERFV
HHHHHCCCCCCCCCCCHHCCCCHHHHHEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
RLEASRTLPGSGLGLSLASAVATLHGGELRLGDARPGLIATLVLPAGAGQPAKLAAPTQD
HHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCC
VPQKAA
CCCCCC
>Mature Secondary Structure 
TAFGKLIRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITEQISTTVSSEIGEMTDI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
YTRGGLRWIVGTIQSRALRPGANLYLLTTPTGQQVAGNVDALAPGVMGSSGWSETFYRRL
HHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHCCCCHHHCCCCCCCCCHHHHHHHHH
DDSSEQNHRALVYVTQLSSGFRLLIGRDLEERRRLFGIVAKAAQWSVLVVIVLGIGGGIF
CCCCCCCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VARRVLYRIDAMTGTTQRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMTGLKE
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSDNIAHDLKTPLTRLRNRAEEALAKSRSEDEYRAALERTIEESDGLIRTFNALLMIARA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
ESGQARGDMVEFDAADVAGGIHELYEPLAEDNEMTLRVKASAAPVHGNRELISQALANLV
CCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
ENAIKYGKPAALPLDPAAAAGSREILIEARREGGQVLLSVTDHGEGIPEAERKHAVERFV
HHHHHCCCCCCCCCCCHHCCCCHHHHHEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHH
RLEASRTLPGSGLGLSLASAVATLHGGELRLGDARPGLIATLVLPAGAGQPAKLAAPTQD
HHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCC
VPQKAA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]