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The map label for this gene is cycJ

Identifier: 146339735

GI number: 146339735

Start: 2935301

End: 2935816

Strand: Direct

Name: cycJ

Synonym: BRADO2733

Alternate gene names: 146339735

Gene position: 2935301-2935816 (Clockwise)

Preceding gene: 146339734

Following gene: 146339736

Centisome position: 39.37

GC content: 67.25

Gene sequence:

>516_bases
ATGACACGCAAGCAGAGGCGTTTGACGATCATCGGCGGGGCGCTGTTCGTGCTCGCGGTCGCGGCCGGGCTGGTGCTGAA
CGCGCTGCGCGACTCCATCGTGTTCTTTTCGACGCCGACCATGGTCGCGGAGAAGCATGTCGCGCCGGGCAAGCGCTTCC
GGCTTGGCGGCCTCGTGCAGCCGGGCTCGTTGAAGCGTGGCGATGATCTCGCGGTGACGTTCGAAGTCGCCGACGGCGGC
GCCAAGCTGCCGGTCGCCTACAAGGGCATCCTGCCGGACCTGTTCCGCGAGGGGCAGGGCGTGGTCGCCGAGGGCGCCCT
CGATGCCTCTGGCGTGTTCAAGGCCGACACCGTGCTCGCCAAGCATGACGAGACCTACATGCCGAAGGAGGTCGCCGACA
CCCTGAAGAAGCAGGGCCATTGGAAGGACGATTACGGCAAGCCGCAAGGCGCGGCCAAGCCGGGCGCGGTATCGATGCGT
GAGGGCGAGAAGACGGCTGCAGGAGCGACGCAGTGA

Upstream 100 bases:

>100_bases
GTGAAGGATGCCGAGCGGTTGCGCCAGCTCAACGAGGGCGTGAAGACGTTCGGGCTGGATGGATGAGGAGCGGCGCATCG
GCCGCGAACGGGATGTTGGG

Downstream 100 bases:

>100_bases
TTGCAGAGAGTGGACATTATGCCCTGGTGCTGGCGCTCGCGCTGGCGCTGATCCAGTCGACGGTGCCGATGCTCGGCGCC
CATCAACGCGACGTCGCGCT

Product: cytochrome c-type biogenesis protein CcmE

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MTRKQRRLTIIGGALFVLAVAAGLVLNALRDSIVFFSTPTMVAEKHVAPGKRFRLGGLVQPGSLKRGDDLAVTFEVADGG
AKLPVAYKGILPDLFREGQGVVAEGALDASGVFKADTVLAKHDETYMPKEVADTLKKQGHWKDDYGKPQGAAKPGAVSMR
EGEKTAAGATQ

Sequences:

>Translated_171_residues
MTRKQRRLTIIGGALFVLAVAAGLVLNALRDSIVFFSTPTMVAEKHVAPGKRFRLGGLVQPGSLKRGDDLAVTFEVADGG
AKLPVAYKGILPDLFREGQGVVAEGALDASGVFKADTVLAKHDETYMPKEVADTLKKQGHWKDDYGKPQGAAKPGAVSMR
EGEKTAAGATQ
>Mature_170_residues
TRKQRRLTIIGGALFVLAVAAGLVLNALRDSIVFFSTPTMVAEKHVAPGKRFRLGGLVQPGSLKRGDDLAVTFEVADGGA
KLPVAYKGILPDLFREGQGVVAEGALDASGVFKADTVLAKHDETYMPKEVADTLKKQGHWKDDYGKPQGAAKPGAVSMRE
GEKTAAGATQ

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=141, Percent_Identity=46.0992907801418, Blast_Score=113, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME_BRASO (A4YRM0)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001204783.1
- ProteinModelPortal:   A4YRM0
- SMR:   A4YRM0
- STRING:   A4YRM0
- GeneID:   5115993
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO2733
- eggNOG:   COG2332
- HOGENOM:   HBG296631
- OMA:   HVEFAVH
- ProtClustDB:   PRK13254
- BioCyc:   BSP376:BRADO2733-MONOMER
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 18076; Mature: 17945

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: BINDING 122-122

Signals:

None

Transmembrane regions:

HASH(0x1a32f28c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKQRRLTIIGGALFVLAVAAGLVLNALRDSIVFFSTPTMVAEKHVAPGKRFRLGGLVQ
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHCCCCCCEEEECCCCC
PGSLKRGDDLAVTFEVADGGAKLPVAYKGILPDLFREGQGVVAEGALDASGVFKADTVLA
CCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHEEE
KHDETYMPKEVADTLKKQGHWKDDYGKPQGAAKPGAVSMREGEKTAAGATQ
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCH
>Mature Secondary Structure 
TRKQRRLTIIGGALFVLAVAAGLVLNALRDSIVFFSTPTMVAEKHVAPGKRFRLGGLVQ
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHCCCCCCEEEECCCCC
PGSLKRGDDLAVTFEVADGGAKLPVAYKGILPDLFREGQGVVAEGALDASGVFKADTVLA
CCCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHEEE
KHDETYMPKEVADTLKKQGHWKDDYGKPQGAAKPGAVSMREGEKTAAGATQ
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA