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The map label for this gene is yisK [H]

Identifier: 146339600

GI number: 146339600

Start: 2776653

End: 2777525

Strand: Reverse

Name: yisK [H]

Synonym: BRADO2592

Alternate gene names: 146339600

Gene position: 2777525-2776653 (Counterclockwise)

Preceding gene: 146339601

Following gene: 146339599

Centisome position: 37.25

GC content: 65.52

Gene sequence:

>873_bases
ATGACCTCCCCGCGCCTCGCGACCTACGCGATCAACGGCTCCGTCCGCTACGGCCTCGTCAACGACAACGGCCTCGTCGA
TCTCTCCGCACGCTTTGCGAAGGACTATCCGACCTTGCGGGAGGTGATCGCCGCGGGCGCCCTGTCCCGGCTGATCGAGG
CCGCCGCCGGCCTGTCGCCGGATCACGGCGTCGATGCCATCACCTGGCAACCGCCGATCCCTTCGCCCGAGAAGATCATC
TGCATCGGCGTCAACTATCCCGACCGCAATGCCGAGTACAAGGACGGCCAGGACGCGCCGAAATATCCGTCGATGTTCAT
GCGCACGCCGCGCTCCTTCGTCGGCCACGAGGCGCCACTGGTGCGCCCGCGCGCTTCGCCGCAACTCGACTACGAGGGCG
AGCTGGTGCTGGTCATCGGCAAGGCCGGCCGGCACATTCCCGAGGCCCAGGCGCTGGATCACATCGCGGCGCTGACCTTG
TGCAACGAAGGCACCATCCGCGACTGGGTGCGGCACGCCAAGTTCAACGTCACACAAGGCAAGAATTTCGATTCCACCGG
CAGCCTCGGGCCCTGGATCGTGCCCTACACCAGTGAGAGCCAGATCGCCGACATCCGCCTGACCACGCACGTCAATGGCG
AACTGCGCCAGGACGACCGGACGTCGCGGCTGATCTTCGGCTTTCGCTATCTCATCAATTACATCTCAACCTTCACGACG
CTCGTTCCCGGTGACGTGATCGTGACGGGCACGCCGACCGGCGCCGGTGCCCGGTTCGATCCGCCGCGCTATCTCAAGCC
CGGCGATGTCATCGAGATCGCCGCCGAGGGCATCGGCACCTTGCGCAACGGCGTCGTCGACGAAGCAGAATAG

Upstream 100 bases:

>100_bases
CGGCGACTATTCGTTCGACTTCTACATGGAGACCAAGCACGTCTCGCTCGCGCGCGGCACCCACAAGATCCAGCGGCTCG
GCGTATAAGGAGACCAGCCC

Downstream 100 bases:

>100_bases
AAACGGACATATCAGCATGACCACCATGACCGGCGGCGAAGCCATCGTCAGCGGGCTCGTCGCCCACGGCGTCGACACCG
TGTTCGGCCTGCCCGGCGCG

Product: putative 2-oxohepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTSPRLATYAINGSVRYGLVNDNGLVDLSARFAKDYPTLREVIAAGALSRLIEAAAGLSPDHGVDAITWQPPIPSPEKII
CIGVNYPDRNAEYKDGQDAPKYPSMFMRTPRSFVGHEAPLVRPRASPQLDYEGELVLVIGKAGRHIPEAQALDHIAALTL
CNEGTIRDWVRHAKFNVTQGKNFDSTGSLGPWIVPYTSESQIADIRLTTHVNGELRQDDRTSRLIFGFRYLINYISTFTT
LVPGDVIVTGTPTGAGARFDPPRYLKPGDVIEIAAEGIGTLRNGVVDEAE

Sequences:

>Translated_290_residues
MTSPRLATYAINGSVRYGLVNDNGLVDLSARFAKDYPTLREVIAAGALSRLIEAAAGLSPDHGVDAITWQPPIPSPEKII
CIGVNYPDRNAEYKDGQDAPKYPSMFMRTPRSFVGHEAPLVRPRASPQLDYEGELVLVIGKAGRHIPEAQALDHIAALTL
CNEGTIRDWVRHAKFNVTQGKNFDSTGSLGPWIVPYTSESQIADIRLTTHVNGELRQDDRTSRLIFGFRYLINYISTFTT
LVPGDVIVTGTPTGAGARFDPPRYLKPGDVIEIAAEGIGTLRNGVVDEAE
>Mature_289_residues
TSPRLATYAINGSVRYGLVNDNGLVDLSARFAKDYPTLREVIAAGALSRLIEAAAGLSPDHGVDAITWQPPIPSPEKIIC
IGVNYPDRNAEYKDGQDAPKYPSMFMRTPRSFVGHEAPLVRPRASPQLDYEGELVLVIGKAGRHIPEAQALDHIAALTLC
NEGTIRDWVRHAKFNVTQGKNFDSTGSLGPWIVPYTSESQIADIRLTTHVNGELRQDDRTSRLIFGFRYLINYISTFTTL
VPGDVIVTGTPTGAGARFDPPRYLKPGDVIEIAAEGIGTLRNGVVDEAE

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI40786394, Length=270, Percent_Identity=35.1851851851852, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI156231349, Length=270, Percent_Identity=35.1851851851852, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI66348062, Length=212, Percent_Identity=33.4905660377358, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI13654274, Length=208, Percent_Identity=32.2115384615385, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI215422413, Length=205, Percent_Identity=32.6829268292683, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1787428, Length=205, Percent_Identity=30.2439024390244, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557057, Length=199, Percent_Identity=34.1708542713568, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324161, Length=230, Percent_Identity=28.695652173913, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28572127, Length=223, Percent_Identity=38.1165919282511, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24663695, Length=277, Percent_Identity=30.6859205776173, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571789, Length=225, Percent_Identity=32.4444444444444, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 31642; Mature: 31511

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPRLATYAINGSVRYGLVNDNGLVDLSARFAKDYPTLREVIAAGALSRLIEAAAGLSP
CCCCCEEEEEECCEEEEEEECCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
DHGVDAITWQPPIPSPEKIICIGVNYPDRNAEYKDGQDAPKYPSMFMRTPRSFVGHEAPL
CCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCE
VRPRASPQLDYEGELVLVIGKAGRHIPEAQALDHIAALTLCNEGTIRDWVRHAKFNVTQG
ECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCC
KNFDSTGSLGPWIVPYTSESQIADIRLTTHVNGELRQDDRTSRLIFGFRYLINYISTFTT
CCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LVPGDVIVTGTPTGAGARFDPPRYLKPGDVIEIAAEGIGTLRNGVVDEAE
HCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSPRLATYAINGSVRYGLVNDNGLVDLSARFAKDYPTLREVIAAGALSRLIEAAAGLSP
CCCCEEEEEECCEEEEEEECCCCEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
DHGVDAITWQPPIPSPEKIICIGVNYPDRNAEYKDGQDAPKYPSMFMRTPRSFVGHEAPL
CCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHCCCCCE
VRPRASPQLDYEGELVLVIGKAGRHIPEAQALDHIAALTLCNEGTIRDWVRHAKFNVTQG
ECCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCC
KNFDSTGSLGPWIVPYTSESQIADIRLTTHVNGELRQDDRTSRLIFGFRYLINYISTFTT
CCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LVPGDVIVTGTPTGAGARFDPPRYLKPGDVIEIAAEGIGTLRNGVVDEAE
HCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]