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The map label for this gene is ilvB [H]

Identifier: 146339599

GI number: 146339599

Start: 2775008

End: 2776636

Strand: Reverse

Name: ilvB [H]

Synonym: BRADO2591

Alternate gene names: 146339599

Gene position: 2776636-2775008 (Counterclockwise)

Preceding gene: 146339600

Following gene: 146339596

Centisome position: 37.24

GC content: 68.14

Gene sequence:

>1629_bases
ATGACCACCATGACCGGCGGCGAAGCCATCGTCAGCGGGCTCGTCGCCCACGGCGTCGACACCGTGTTCGGCCTGCCCGG
CGCGCAGGTCTACGGCCTGTTCGACGCCTTCCACCAGGCGCAACTGAAGGTCGTCGGCGCGCGCCATGAGCAGGCCTGCG
GCTACATGGCGTTCGGCTACGCCCGCGCCAGCGGCAGGCCCGGCGTGTTCAGCGTCGTCCCAGGCCCCGGCGTGCTCAAT
GCCGGCGCGGCGCTGCTCACCGCTTTCGGCTGCAACGAGCCGGTGCTGTGCCTGACCGGCCAGGTGCCGACGGACTATCT
CGGCCGCGGCCGCGGCCATCTGCACGAGATGCCCGACCAGCTCGCCACTTTACGCACCTTCGTCAAATGGGCCGAGCGCA
TCGAATATCCCGACAGCGCGCCGACACTGGTCGCGCGCGCGTTCCAGGAGATGCTGTCCGGACGGCGCGGGCCCGCCGCG
CTGGAGATGCCGTGGGACGTGTTCACGCAGAAGGCCGCGACATCGGCCGCAAATCCGCTGGCTCAGGTCACGCCACCGCA
GCCCGATCCCGACCGCATCGCCAAGGCCACCGCCCTCATCGCGAGCGCCAAGGCACCGATGATCTTCGTCGGCTCCGGCG
CGATCGAGGCGCGCGAGGAGATCCTCGAACTGGCCGAGATGATCGACGCGCCGGTCGTCGCCTTCCGCTCGGGGCGCGGC
ATCGTCTCCAACGCGCATGAGCTCGGGCTCACCATGGCCGCGGCCTACAAGCTCTGGCCAACCACCGATCTGATGATCGG
CATCGGCACCCGCATGGAACTGCCGACGACGTTCCGCTGGCCGTTCAAGCCTGACGGCTTGAAGTCGATCCGCATCGACA
TCGACCCAGCCGAGATGCGTCGCCTGATCGTCGACGCCGGCCTCGTCGCCGATGCCAAGGCGGGCACGGCTGCGCTGGCC
GCGGCTGTGAGCAAGGCGGGCACTCGCAAGACCTCCGGCCGTCGCGCAGCAATCCGCAAGGCCAGCGCTGCGACCTTGCA
GGAGATCCAGAAGGTGCAGCCGCAGATGGCCTACCTCAACGTGCTGCGCGAGGTTTTGCCGCATGACGCCATCGTCACCG
ACGAATTGTCCCAGGTCGGATTCGCCTCCTGGTACGGCTTCCCGGTCTACCAGCCGCGCACCTTCATCACGTCAGGCTAT
CAGGGCACGCTGGGCGCCGGCTTTCCGACCGCCCTCGGCGCCAAGGTCGCGCATCCCGACAAGCCGGTGGTCGCGATCAC
CGGCGATGGCGGATTCATGTTCGCGGTGCAGGAGCTGGCGACCGCCGTGCAATACAACATCGGCGTCGTGACCCTGGTGT
TCAACAACAACTCCTACGGCAATGTCCGGCGCGACCAGCGCGAACGCTTCGACGGCCGCGTCGTCGCCTCCGACCTGGTC
AATCCGGATTTCGTCAAGCTCGCGGAGTCGTTCGGTGTTGCGGCAAGCCGCGTCACCGCCCCTGACCAGTTCAAGGCGGC
GCTCGAGACCGCACTCGCGCATGGCGGGCCGTATCTGATCGACATCGAGGTGCCGCGCGACAGCGAAGTCTCGCCCTGGG
CCTTCATTCATCCGGCCAAGCCGGCCTGA

Upstream 100 bases:

>100_bases
TATCTCAAGCCCGGCGATGTCATCGAGATCGCCGCCGAGGGCATCGGCACCTTGCGCAACGGCGTCGTCGACGAAGCAGA
ATAGAAACGGACATATCAGC

Downstream 100 bases:

>100_bases
TCAACCCGCGCGGCGAAAGGTCAGGTTGATACGCCGGCGGCCAAGCCGCGCATGCTCGCCGTCGGCGAGCGGCGCGACGC
CGTGAAACGCGAGCCGCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MTTMTGGEAIVSGLVAHGVDTVFGLPGAQVYGLFDAFHQAQLKVVGARHEQACGYMAFGYARASGRPGVFSVVPGPGVLN
AGAALLTAFGCNEPVLCLTGQVPTDYLGRGRGHLHEMPDQLATLRTFVKWAERIEYPDSAPTLVARAFQEMLSGRRGPAA
LEMPWDVFTQKAATSAANPLAQVTPPQPDPDRIAKATALIASAKAPMIFVGSGAIEAREEILELAEMIDAPVVAFRSGRG
IVSNAHELGLTMAAAYKLWPTTDLMIGIGTRMELPTTFRWPFKPDGLKSIRIDIDPAEMRRLIVDAGLVADAKAGTAALA
AAVSKAGTRKTSGRRAAIRKASAATLQEIQKVQPQMAYLNVLREVLPHDAIVTDELSQVGFASWYGFPVYQPRTFITSGY
QGTLGAGFPTALGAKVAHPDKPVVAITGDGGFMFAVQELATAVQYNIGVVTLVFNNNSYGNVRRDQRERFDGRVVASDLV
NPDFVKLAESFGVAASRVTAPDQFKAALETALAHGGPYLIDIEVPRDSEVSPWAFIHPAKPA

Sequences:

>Translated_542_residues
MTTMTGGEAIVSGLVAHGVDTVFGLPGAQVYGLFDAFHQAQLKVVGARHEQACGYMAFGYARASGRPGVFSVVPGPGVLN
AGAALLTAFGCNEPVLCLTGQVPTDYLGRGRGHLHEMPDQLATLRTFVKWAERIEYPDSAPTLVARAFQEMLSGRRGPAA
LEMPWDVFTQKAATSAANPLAQVTPPQPDPDRIAKATALIASAKAPMIFVGSGAIEAREEILELAEMIDAPVVAFRSGRG
IVSNAHELGLTMAAAYKLWPTTDLMIGIGTRMELPTTFRWPFKPDGLKSIRIDIDPAEMRRLIVDAGLVADAKAGTAALA
AAVSKAGTRKTSGRRAAIRKASAATLQEIQKVQPQMAYLNVLREVLPHDAIVTDELSQVGFASWYGFPVYQPRTFITSGY
QGTLGAGFPTALGAKVAHPDKPVVAITGDGGFMFAVQELATAVQYNIGVVTLVFNNNSYGNVRRDQRERFDGRVVASDLV
NPDFVKLAESFGVAASRVTAPDQFKAALETALAHGGPYLIDIEVPRDSEVSPWAFIHPAKPA
>Mature_541_residues
TTMTGGEAIVSGLVAHGVDTVFGLPGAQVYGLFDAFHQAQLKVVGARHEQACGYMAFGYARASGRPGVFSVVPGPGVLNA
GAALLTAFGCNEPVLCLTGQVPTDYLGRGRGHLHEMPDQLATLRTFVKWAERIEYPDSAPTLVARAFQEMLSGRRGPAAL
EMPWDVFTQKAATSAANPLAQVTPPQPDPDRIAKATALIASAKAPMIFVGSGAIEAREEILELAEMIDAPVVAFRSGRGI
VSNAHELGLTMAAAYKLWPTTDLMIGIGTRMELPTTFRWPFKPDGLKSIRIDIDPAEMRRLIVDAGLVADAKAGTAALAA
AVSKAGTRKTSGRRAAIRKASAATLQEIQKVQPQMAYLNVLREVLPHDAIVTDELSQVGFASWYGFPVYQPRTFITSGYQ
GTLGAGFPTALGAKVAHPDKPVVAITGDGGFMFAVQELATAVQYNIGVVTLVFNNNSYGNVRRDQRERFDGRVVASDLVN
PDFVKLAESFGVAASRVTAPDQFKAALETALAHGGPYLIDIEVPRDSEVSPWAFIHPAKPA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=551, Percent_Identity=25.2268602540835, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI21361361, Length=582, Percent_Identity=26.9759450171821, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI87081685, Length=571, Percent_Identity=29.7723292469352, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=28.3858998144712, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786717, Length=576, Percent_Identity=30.9027777777778, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1787096, Length=541, Percent_Identity=25.8780036968577, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788716, Length=542, Percent_Identity=26.1992619926199, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=563, Percent_Identity=28.2415630550622, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=26.6903914590747, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=26.6903914590747, Blast_Score=134, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=567, Percent_Identity=30.6878306878307, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=26.1758691206544, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=27.3049645390071, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57739; Mature: 57608

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMTGGEAIVSGLVAHGVDTVFGLPGAQVYGLFDAFHQAQLKVVGARHEQACGYMAFGY
CCCCCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHEEEECCCHHHHCCEEEEEE
ARASGRPGVFSVVPGPGVLNAGAALLTAFGCNEPVLCLTGQVPTDYLGRGRGHLHEMPDQ
EECCCCCCEEEECCCCCCHHCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCHHHHHHHH
LATLRTFVKWAERIEYPDSAPTLVARAFQEMLSGRRGPAALEMPWDVFTQKAATSAANPL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCC
AQVTPPQPDPDRIAKATALIASAKAPMIFVGSGAIEAREEILELAEMIDAPVVAFRSGRG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCC
IVSNAHELGLTMAAAYKLWPTTDLMIGIGTRMELPTTFRWPFKPDGLKSIRIDIDPAEMR
CCCCHHHHHHHHHHHEEECCCCCEEEECCCCEECCCEEECCCCCCCCCEEEEECCHHHHH
RLIVDAGLVADAKAGTAALAAAVSKAGTRKTSGRRAAIRKASAATLQEIQKVQPQMAYLN
HHHHHCCCEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHH
VLREVLPHDAIVTDELSQVGFASWYGFPVYQPRTFITSGYQGTLGAGFPTALGAKVAHPD
HHHHHCCCCCEEHHHHHHCCHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCC
KPVVAITGDGGFMFAVQELATAVQYNIGVVTLVFNNNSYGNVRRDQRERFDGRVVASDLV
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCEEEHHHCC
NPDFVKLAESFGVAASRVTAPDQFKAALETALAHGGPYLIDIEVPRDSEVSPWAFIHPAK
CHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCC
PA
CC
>Mature Secondary Structure 
TTMTGGEAIVSGLVAHGVDTVFGLPGAQVYGLFDAFHQAQLKVVGARHEQACGYMAFGY
CCCCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHEEEECCCHHHHCCEEEEEE
ARASGRPGVFSVVPGPGVLNAGAALLTAFGCNEPVLCLTGQVPTDYLGRGRGHLHEMPDQ
EECCCCCCEEEECCCCCCHHCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCHHHHHHHH
LATLRTFVKWAERIEYPDSAPTLVARAFQEMLSGRRGPAALEMPWDVFTQKAATSAANPL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCC
AQVTPPQPDPDRIAKATALIASAKAPMIFVGSGAIEAREEILELAEMIDAPVVAFRSGRG
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCCC
IVSNAHELGLTMAAAYKLWPTTDLMIGIGTRMELPTTFRWPFKPDGLKSIRIDIDPAEMR
CCCCHHHHHHHHHHHEEECCCCCEEEECCCCEECCCEEECCCCCCCCCEEEEECCHHHHH
RLIVDAGLVADAKAGTAALAAAVSKAGTRKTSGRRAAIRKASAATLQEIQKVQPQMAYLN
HHHHHCCCEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHH
VLREVLPHDAIVTDELSQVGFASWYGFPVYQPRTFITSGYQGTLGAGFPTALGAKVAHPD
HHHHHCCCCCEEHHHHHHCCHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCCCC
KPVVAITGDGGFMFAVQELATAVQYNIGVVTLVFNNNSYGNVRRDQRERFDGRVVASDLV
CCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCEEEHHHCC
NPDFVKLAESFGVAASRVTAPDQFKAALETALAHGGPYLIDIEVPRDSEVSPWAFIHPAK
CHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCCC
PA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]