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The map label for this gene is mrcA [H]

Identifier: 146339596

GI number: 146339596

Start: 2772681

End: 2773439

Strand: Reverse

Name: mrcA [H]

Synonym: BRADO2588

Alternate gene names: 146339596

Gene position: 2773439-2772681 (Counterclockwise)

Preceding gene: 146339599

Following gene: 146339593

Centisome position: 37.19

GC content: 63.77

Gene sequence:

>759_bases
ATGCACCATCCACGCACCCGGCGCTTTCTCATTCTGTTCGGCAAGTCGATTCTGGCCGTGCTCTCGCTCGCCGTCATGCT
GCTGCTTTACGCCGCCGGCTATGCGATCTGGTACGCCGAATATCACCTCGGCGTCCCTACCGAAGCCGAGATCGCGGCGC
TGCCCGGCACGGGCCATCTATGTCCGCTCGAAGCAGGATCGCCCTACATGCCGCTGGCCGACATGCCCCCGCTGCTGCGC
CAAGCCGTCGTCGCGTCGACGGGCTCCGACTTTCAGACGCGACCCAATCTCGTTCTGCCGGCGCTATTGGCGGCCGTTGT
CAAGAGTGACGGACGGCCCGATACGAGGATCACGTTTGCGGTCACCCGCCATTGCCTGCATGTCCTGGCTTCAGACTGTT
GCCGCGGCCTGGATTGGCACATCGGAAGCACGGTCTTCATGGGCCGTCTGGAACGAACCTTGTCGCGCGATCGAATTCTG
GAATCCTACCTGAACGACAGCTATCTCGGCCGCAACGCCTACGGCGTCGCAGCGGCGGCAACCGCGCATTTCGGCAAGCC
CCTGACAGACCTCGACATCGGCGAGATCGCCCTGCTCATCGCGCGTTTCCAGGTGCCGACGCCCTCTGAACGCGACAGCG
AGCGACGCAGTTACATCCTCGACAAAATGCTGTCAGCCGGCCTCATCGACGAGGCACAGGCGAGCGCCGCCAAGGCGACA
CCACTGTCCTTGATCGCTGCCCCGCCCCACAATCCCTGA

Upstream 100 bases:

>100_bases
GAATGAGCGCCGACCGCCACCCGTTTCCAGGATGCTTGGCATCGCGCCGGAACTCCCCCGTTGAACCGGAACGCTTCGGG
CGGAACCAAATCCTCTGACG

Downstream 100 bases:

>100_bases
TCGCTTGCTGCCGCCTCACCCCCGCACGACGCCCTCGATCCCCGCCGCGATCTCCAGCAGCTTGCGGTCGTTGCCGCCGG
ACTGCGCCAGCATCAGGCCG

Product: putative penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MHHPRTRRFLILFGKSILAVLSLAVMLLLYAAGYAIWYAEYHLGVPTEAEIAALPGTGHLCPLEAGSPYMPLADMPPLLR
QAVVASTGSDFQTRPNLVLPALLAAVVKSDGRPDTRITFAVTRHCLHVLASDCCRGLDWHIGSTVFMGRLERTLSRDRIL
ESYLNDSYLGRNAYGVAAAATAHFGKPLTDLDIGEIALLIARFQVPTPSERDSERRSYILDKMLSAGLIDEAQASAAKAT
PLSLIAAPPHNP

Sequences:

>Translated_252_residues
MHHPRTRRFLILFGKSILAVLSLAVMLLLYAAGYAIWYAEYHLGVPTEAEIAALPGTGHLCPLEAGSPYMPLADMPPLLR
QAVVASTGSDFQTRPNLVLPALLAAVVKSDGRPDTRITFAVTRHCLHVLASDCCRGLDWHIGSTVFMGRLERTLSRDRIL
ESYLNDSYLGRNAYGVAAAATAHFGKPLTDLDIGEIALLIARFQVPTPSERDSERRSYILDKMLSAGLIDEAQASAAKAT
PLSLIAAPPHNP
>Mature_252_residues
MHHPRTRRFLILFGKSILAVLSLAVMLLLYAAGYAIWYAEYHLGVPTEAEIAALPGTGHLCPLEAGSPYMPLADMPPLLR
QAVVASTGSDFQTRPNLVLPALLAAVVKSDGRPDTRITFAVTRHCLHVLASDCCRGLDWHIGSTVFMGRLERTLSRDRIL
ESYLNDSYLGRNAYGVAAAATAHFGKPLTDLDIGEIALLIARFQVPTPSERDSERRSYILDKMLSAGLIDEAQASAAKAT
PLSLIAAPPHNP

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=96, Percent_Identity=39.5833333333333, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 27339; Mature: 27339

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHPRTRRFLILFGKSILAVLSLAVMLLLYAAGYAIWYAEYHLGVPTEAEIAALPGTGHL
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEHCCCCCCCCCEEECCCCCCE
CPLEAGSPYMPLADMPPLLRQAVVASTGSDFQTRPNLVLPALLAAVVKSDGRPDTRITFA
EECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
VTRHCLHVLASDCCRGLDWHIGSTVFMGRLERTLSRDRILESYLNDSYLGRNAYGVAAAA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHH
TAHFGKPLTDLDIGEIALLIARFQVPTPSERDSERRSYILDKMLSAGLIDEAQASAAKAT
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PLSLIAAPPHNP
CEEEEECCCCCC
>Mature Secondary Structure
MHHPRTRRFLILFGKSILAVLSLAVMLLLYAAGYAIWYAEYHLGVPTEAEIAALPGTGHL
CCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEHCCCCCCCCCEEECCCCCCE
CPLEAGSPYMPLADMPPLLRQAVVASTGSDFQTRPNLVLPALLAAVVKSDGRPDTRITFA
EECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
VTRHCLHVLASDCCRGLDWHIGSTVFMGRLERTLSRDRILESYLNDSYLGRNAYGVAAAA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHH
TAHFGKPLTDLDIGEIALLIARFQVPTPSERDSERRSYILDKMLSAGLIDEAQASAAKAT
HHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCC
PLSLIAAPPHNP
CEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7901733 [H]