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The map label for this gene is dppA [H]

Identifier: 146339522

GI number: 146339522

Start: 2685665

End: 2687260

Strand: Reverse

Name: dppA [H]

Synonym: BRADO2512

Alternate gene names: 146339522

Gene position: 2687260-2685665 (Counterclockwise)

Preceding gene: 146339525

Following gene: 146339521

Centisome position: 36.04

GC content: 62.84

Gene sequence:

>1596_bases
ATGTCGGGACGCAAATCCCTGCTCATCGCCGCAGCGGTCGCCTTCGGCCTCTCCCTCATGCCGGCTTCGGCCCAGACCCT
GCGCTATGCCAACCAGGGCACCCTGAAGTCGCTCGATCCCTACACCTTCAAGGAATCAACCACGATTGCGCATCACGCGC
ACGTCTATGAAGGGCTCACGGCGCGCGACAAGGATCTCAAGATCATTCCGGCCCTTGCCGAGAGCTGGGAAACGCCCGAG
CCGACCCGCTGGCGCTTTCATCTGCGCAAGGGTGTCAAGTTCCACAATGGTGAACCCTTCACCGCCGACGACGTGATCTT
CTCCGCCGACCGCGTCCGCGCCACCGGCTCCAACTTTCTGTCCAACGTTCCGCCGGACGCCAAGTTCATCAAGGTGGACG
ACTACACGGTGGACGTGAAGCTCGACGCGCCCAATCCCATTCTGATCTCGCAATGGGATACGTGGTTCATCATGAGCAAG
AAGTGGTGCGAGGACAACAACGCGGTCGCGCCGACCCCGGTCTCCGCGACCACGCCGAGCTACGCCTCGCTGCACGAGAA
CGGCACCGGTCCCTTTATCATCGAGAGCCATCAGCCGGGCGTGAAGACCGTCTTCAAGGTCAACCCGAACTGGTGGGGCA
AGCCGGAGCATAATCTCAAGGAGATCGTGTTCACGACCATTTCCTCGGACGCGACGCGCGTCGCGGCGCTGCTCTCCGGC
GAGGTCGACATCATCGAGCCGGTTCCGGTGCAGGACATCGGCCGTGTCGATTCCAGCCCGAATGCGCAGGTCCTGAAGGG
ACCCGAGCTGCGCACGATCTTCCTCGGCATGGACGAGACGCGCGATGAGCTGCTCTACTCCAACGTCAAGGGCAAGAACC
CGTTCAAGGACATCCGTGTCCGCGAGGCGTTCTACAAGTCCATCGACGTCGAGCTGATCAAGACCCGAGTCATGCGCGGC
CTGTCGACGCCGTCGGCGCTGATGATCGCGCCGCAGCTGTATTCGCTGTCGAAGGACTTCACCCGTCCGAAATACGATCC
GGACGCGGCCAAGAAGCTCCTGACCGAGGCCGGCTATCCCGACGGCTTCGAGGTCGGCATGGACTGCCCGAACGACCGCT
ACGTCAACGACGCCGCGATCTGCCAGGCCGTCGTCGGCATGCTGGCGCGCATCGGCGTGAAGGTGAACCTGAACGCGCAG
CCCAAGGCGCAGTATTTCGCGAAGGTGCTGAAGCCCGGCGGCTACAACACCTCCTTCTTCCTGCTCGGCTGGACGCCCTC
GTCGATGGACGCGCACAACGTGATGCACGACATCATGGGCTGCCGTGACGACGCCAAGGATCCGACCCGCGGCGAGGCCA
ATCTCGGCAACTACTGCAACAAGGAGTTCGACGCGCTCACCGACAAGGTCCTGGTCGAAGCCGACCCGGCGAAGCGCGAT
CAGCTGATCAAGCAGGCCTTCGAGATCGGCATCAAGGACTACGGCTACATCCCGCTGCATCAGCAGGCCGTGGCCTGGGG
CGTGTCGAAGAAGGTCAAGATCCCGCTGCGCGCCGACAACGCCGTGCTGATGTTCTGGGCCAACAAGACCGAGTAG

Upstream 100 bases:

>100_bases
GTGCAAGAGGCGCTGCAGACATCACGAACTTCGAAGCAAGGCAGGCGGGACCCAGACGCCTGCCAAAAAACCAAGCGTCA
AACGCGGAGAATTTGGACAT

Downstream 100 bases:

>100_bases
TTCGCCCGACGACCCAAAGTCCCGGAAAGCAGAACTGCTTTTCGGGACTTTTCATTTTCCACGGCAGGTGCTACCCGCCG
CGCGATCGAGAGGACTGAAA

Product: putative oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSGRKSLLIAAAVAFGLSLMPASAQTLRYANQGTLKSLDPYTFKESTTIAHHAHVYEGLTARDKDLKIIPALAESWETPE
PTRWRFHLRKGVKFHNGEPFTADDVIFSADRVRATGSNFLSNVPPDAKFIKVDDYTVDVKLDAPNPILISQWDTWFIMSK
KWCEDNNAVAPTPVSATTPSYASLHENGTGPFIIESHQPGVKTVFKVNPNWWGKPEHNLKEIVFTTISSDATRVAALLSG
EVDIIEPVPVQDIGRVDSSPNAQVLKGPELRTIFLGMDETRDELLYSNVKGKNPFKDIRVREAFYKSIDVELIKTRVMRG
LSTPSALMIAPQLYSLSKDFTRPKYDPDAAKKLLTEAGYPDGFEVGMDCPNDRYVNDAAICQAVVGMLARIGVKVNLNAQ
PKAQYFAKVLKPGGYNTSFFLLGWTPSSMDAHNVMHDIMGCRDDAKDPTRGEANLGNYCNKEFDALTDKVLVEADPAKRD
QLIKQAFEIGIKDYGYIPLHQQAVAWGVSKKVKIPLRADNAVLMFWANKTE

Sequences:

>Translated_531_residues
MSGRKSLLIAAAVAFGLSLMPASAQTLRYANQGTLKSLDPYTFKESTTIAHHAHVYEGLTARDKDLKIIPALAESWETPE
PTRWRFHLRKGVKFHNGEPFTADDVIFSADRVRATGSNFLSNVPPDAKFIKVDDYTVDVKLDAPNPILISQWDTWFIMSK
KWCEDNNAVAPTPVSATTPSYASLHENGTGPFIIESHQPGVKTVFKVNPNWWGKPEHNLKEIVFTTISSDATRVAALLSG
EVDIIEPVPVQDIGRVDSSPNAQVLKGPELRTIFLGMDETRDELLYSNVKGKNPFKDIRVREAFYKSIDVELIKTRVMRG
LSTPSALMIAPQLYSLSKDFTRPKYDPDAAKKLLTEAGYPDGFEVGMDCPNDRYVNDAAICQAVVGMLARIGVKVNLNAQ
PKAQYFAKVLKPGGYNTSFFLLGWTPSSMDAHNVMHDIMGCRDDAKDPTRGEANLGNYCNKEFDALTDKVLVEADPAKRD
QLIKQAFEIGIKDYGYIPLHQQAVAWGVSKKVKIPLRADNAVLMFWANKTE
>Mature_530_residues
SGRKSLLIAAAVAFGLSLMPASAQTLRYANQGTLKSLDPYTFKESTTIAHHAHVYEGLTARDKDLKIIPALAESWETPEP
TRWRFHLRKGVKFHNGEPFTADDVIFSADRVRATGSNFLSNVPPDAKFIKVDDYTVDVKLDAPNPILISQWDTWFIMSKK
WCEDNNAVAPTPVSATTPSYASLHENGTGPFIIESHQPGVKTVFKVNPNWWGKPEHNLKEIVFTTISSDATRVAALLSGE
VDIIEPVPVQDIGRVDSSPNAQVLKGPELRTIFLGMDETRDELLYSNVKGKNPFKDIRVREAFYKSIDVELIKTRVMRGL
STPSALMIAPQLYSLSKDFTRPKYDPDAAKKLLTEAGYPDGFEVGMDCPNDRYVNDAAICQAVVGMLARIGVKVNLNAQP
KAQYFAKVLKPGGYNTSFFLLGWTPSSMDAHNVMHDIMGCRDDAKDPTRGEANLGNYCNKEFDALTDKVLVEADPAKRDQ
LIKQAFEIGIKDYGYIPLHQQAVAWGVSKKVKIPLRADNAVLMFWANKTE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=519, Percent_Identity=27.5529865125241, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787052, Length=512, Percent_Identity=24.21875, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789397, Length=525, Percent_Identity=25.3333333333333, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787495, Length=532, Percent_Identity=22.9323308270677, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789887, Length=335, Percent_Identity=25.6716417910448, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787762, Length=445, Percent_Identity=24.4943820224719, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081878, Length=440, Percent_Identity=23.8636363636364, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787551, Length=462, Percent_Identity=20.1298701298701, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59013; Mature: 58882

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRKSLLIAAAVAFGLSLMPASAQTLRYANQGTLKSLDPYTFKESTTIAHHAHVYEGLT
CCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
ARDKDLKIIPALAESWETPEPTRWRFHLRKGVKFHNGEPFTADDVIFSADRVRATGSNFL
CCCCCCEEEEHHHHCCCCCCCCCEEEEECCCCEECCCCCCCHHHEEEECCHHEECCCHHH
SNVPPDAKFIKVDDYTVDVKLDAPNPILISQWDTWFIMSKKWCEDNNAVAPTPVSATTPS
HCCCCCCEEEEEECEEEEEEECCCCCEEEECCCEEEEEEHHCCCCCCCCCCCCCCCCCCC
YASLHENGTGPFIIESHQPGVKTVFKVNPNWWGKPEHNLKEIVFTTISSDATRVAALLSG
HHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHEEEEECCCCHHHHHHHHCC
EVDIIEPVPVQDIGRVDSSPNAQVLKGPELRTIFLGMDETRDELLYSNVKGKNPFKDIRV
CCEEECCCCHHHHHCCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHH
REAFYKSIDVELIKTRVMRGLSTPSALMIAPQLYSLSKDFTRPKYDPDAAKKLLTEAGYP
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
DGFEVGMDCPNDRYVNDAAICQAVVGMLARIGVKVNLNAQPKAQYFAKVLKPGGYNTSFF
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCCEEE
LLGWTPSSMDAHNVMHDIMGCRDDAKDPTRGEANLGNYCNKEFDALTDKVLVEADPAKRD
EEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHEEEECCCHHHH
QLIKQAFEIGIKDYGYIPLHQQAVAWGVSKKVKIPLRADNAVLMFWANKTE
HHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCEEEEEEECCCC
>Mature Secondary Structure 
SGRKSLLIAAAVAFGLSLMPASAQTLRYANQGTLKSLDPYTFKESTTIAHHAHVYEGLT
CCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCC
ARDKDLKIIPALAESWETPEPTRWRFHLRKGVKFHNGEPFTADDVIFSADRVRATGSNFL
CCCCCCEEEEHHHHCCCCCCCCCEEEEECCCCEECCCCCCCHHHEEEECCHHEECCCHHH
SNVPPDAKFIKVDDYTVDVKLDAPNPILISQWDTWFIMSKKWCEDNNAVAPTPVSATTPS
HCCCCCCEEEEEECEEEEEEECCCCCEEEECCCEEEEEEHHCCCCCCCCCCCCCCCCCCC
YASLHENGTGPFIIESHQPGVKTVFKVNPNWWGKPEHNLKEIVFTTISSDATRVAALLSG
HHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHEEEEECCCCHHHHHHHHCC
EVDIIEPVPVQDIGRVDSSPNAQVLKGPELRTIFLGMDETRDELLYSNVKGKNPFKDIRV
CCEEECCCCHHHHHCCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHH
REAFYKSIDVELIKTRVMRGLSTPSALMIAPQLYSLSKDFTRPKYDPDAAKKLLTEAGYP
HHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
DGFEVGMDCPNDRYVNDAAICQAVVGMLARIGVKVNLNAQPKAQYFAKVLKPGGYNTSFF
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHCCCCCCCEEE
LLGWTPSSMDAHNVMHDIMGCRDDAKDPTRGEANLGNYCNKEFDALTDKVLVEADPAKRD
EEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHEEEECCCHHHH
QLIKQAFEIGIKDYGYIPLHQQAVAWGVSKKVKIPLRADNAVLMFWANKTE
HHHHHHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]