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The map label for this gene is fixJ [H]

Identifier: 146339448

GI number: 146339448

Start: 2594593

End: 2595210

Strand: Reverse

Name: fixJ [H]

Synonym: BRADO2434

Alternate gene names: 146339448

Gene position: 2595210-2594593 (Counterclockwise)

Preceding gene: 146339449

Following gene: 146339447

Centisome position: 34.8

GC content: 63.92

Gene sequence:

>618_bases
ATGGCTGAACGGGGAAGGGTCTATGTGATCGACGACGACGAGGCGATGCGGGATTCGCTCAACTTCCTGCTCGATTCCTC
GGGCTTCGACGTCACGCTGTTCGAGAATGCGCAGTCCTTCCTCGATCGGTTGCCGACGCTCGGCTTCGGCTGCGTCGTCT
CCGACGTGCGGATGCCGGGCATCGACGGCATCGAGCTGCTCAAGCGCATGAAGACGACAGGCAGCGGCTTCCCTATCCTG
GTGATGACCGGCCACGGCGACGTGCCGCTTGCGGTCGAGGCGATGAAGCTCGGCGCCGTCGACTTTCTCGAAAAGCCGTT
CGAGGACGAGCGCCTCGTCGCGATGATCGAAACGGCGATCAAGCAGGCTGAACCTGCGGCGAAGAACGAGTCTGTGACGC
AGGACATTCTCGCCCGCGTCGCGAGTCTCAGCCCGCGCGAGCGCCAGGTGATGGACGGCCTGATGGCCGGCCTCTCCAAC
AAGCTCATCGCGCGCGAATACGACATCAGCCCGCGCACGATCGAGGTCTACCGCGCCAACGTGATGACCAAGATGGGCGC
GAACAGCCTCTCCGAGCTGGTCCGTCTGGCGATGCGCGCCGGCGCGTTGAAGGATTGA

Upstream 100 bases:

>100_bases
ATCGAGGCCCATGGCGGGCGCATGTGGGCCGAAAACAACGAGGCAGGCGGCGCGACATTCCGCTTCACCCTGCCCGCCGC
TCCAACCAAGGTTGAGCATG

Downstream 100 bases:

>100_bases
GCTAGATCAAGCCGGCTTGACAGAATCGAGTGTTATGTTGTGCCTCGGTTCAACATGAACTTCTCGCGGCACGTGCGATG
TCGGCTCTCGCCAAACCAAA

Product: response regulator FixJ

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MAERGRVYVIDDDEAMRDSLNFLLDSSGFDVTLFENAQSFLDRLPTLGFGCVVSDVRMPGIDGIELLKRMKTTGSGFPIL
VMTGHGDVPLAVEAMKLGAVDFLEKPFEDERLVAMIETAIKQAEPAAKNESVTQDILARVASLSPRERQVMDGLMAGLSN
KLIAREYDISPRTIEVYRANVMTKMGANSLSELVRLAMRAGALKD

Sequences:

>Translated_205_residues
MAERGRVYVIDDDEAMRDSLNFLLDSSGFDVTLFENAQSFLDRLPTLGFGCVVSDVRMPGIDGIELLKRMKTTGSGFPIL
VMTGHGDVPLAVEAMKLGAVDFLEKPFEDERLVAMIETAIKQAEPAAKNESVTQDILARVASLSPRERQVMDGLMAGLSN
KLIAREYDISPRTIEVYRANVMTKMGANSLSELVRLAMRAGALKD
>Mature_204_residues
AERGRVYVIDDDEAMRDSLNFLLDSSGFDVTLFENAQSFLDRLPTLGFGCVVSDVRMPGIDGIELLKRMKTTGSGFPILV
MTGHGDVPLAVEAMKLGAVDFLEKPFEDERLVAMIETAIKQAEPAAKNESVTQDILARVASLSPRERQVMDGLMAGLSNK
LIAREYDISPRTIEVYRANVMTKMGANSLSELVRLAMRAGALKD

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=147, Percent_Identity=36.0544217687075, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790437, Length=178, Percent_Identity=32.0224719101124, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=32.6633165829146, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789402, Length=203, Percent_Identity=28.0788177339901, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788222, Length=216, Percent_Identity=28.2407407407407, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788905, Length=135, Percent_Identity=31.1111111111111, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=31.7073170731707, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786599, Length=150, Percent_Identity=28, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1786747, Length=201, Percent_Identity=25.8706467661692, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1790860, Length=122, Percent_Identity=25.4098360655738, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788191, Length=105, Percent_Identity=35.2380952380952, Blast_Score=61, Evalue=4e-11,
Organism=Escherichia coli, GI1788712, Length=167, Percent_Identity=29.3413173652695, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22487; Mature: 22356

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERGRVYVIDDDEAMRDSLNFLLDSSGFDVTLFENAQSFLDRLPTLGFGCVVSDVRMPG
CCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHCCCCCC
IDGIELLKRMKTTGSGFPILVMTGHGDVPLAVEAMKLGAVDFLEKPFEDERLVAMIETAI
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KQAEPAAKNESVTQDILARVASLSPRERQVMDGLMAGLSNKLIAREYDISPRTIEVYRAN
HHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEECCCCCCEEEHHHHH
VMTKMGANSLSELVRLAMRAGALKD
HHHHHCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AERGRVYVIDDDEAMRDSLNFLLDSSGFDVTLFENAQSFLDRLPTLGFGCVVSDVRMPG
CCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHCCCCCC
IDGIELLKRMKTTGSGFPILVMTGHGDVPLAVEAMKLGAVDFLEKPFEDERLVAMIETAI
CCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
KQAEPAAKNESVTQDILARVASLSPRERQVMDGLMAGLSNKLIAREYDISPRTIEVYRAN
HHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEECCCCCCEEEHHHHH
VMTKMGANSLSELVRLAMRAGALKD
HHHHHCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]