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The map label for this gene is fixJ [H]

Identifier: 146339447

GI number: 146339447

Start: 2594120

End: 2594515

Strand: Reverse

Name: fixJ [H]

Synonym: BRADO2433

Alternate gene names: 146339447

Gene position: 2594515-2594120 (Counterclockwise)

Preceding gene: 146339448

Following gene: 146339446

Centisome position: 34.79

GC content: 63.38

Gene sequence:

>396_bases
ATGTCGGCTCTCGCCAAACCAAAGGTCTATGTAGCGGACGACGATGCCGACGTTCTCGGCTCGCTGCGATTCCTGCTGGA
GGCCGACGGATTCGACGTCCGCACGTTCCGCAGCGGCAACGCGCTGCTGAAGGCATCGGATCGCGCGGCAGTGGACTGCT
TCGTGGTCGATTACAAGATGCCCGAGTTGAATGGCGTCGAGGTCGCCGACAGGTTGCGCAAGCAAGGCGCGACAGCTCCC
GTGGTGCTCATCACCGGCCATTATGACGACCGCCTGGCGGCACGCGCCGCAGCCGTCGGCATCCAGGATTTCCTGCTGAA
GCCGCTGCTCGACGACAATCTGGTCAAGCGGATCCGCAAGGCGATCGCCAATGACGAGCCGGTTACCGCTCCCTGA

Upstream 100 bases:

>100_bases
GCGCCGGCGCGTTGAAGGATTGAGCTAGATCAAGCCGGCTTGACAGAATCGAGTGTTATGTTGTGCCTCGGTTCAACATG
AACTTCTCGCGGCACGTGCG

Downstream 100 bases:

>100_bases
GCCCGACGGCCGCCGATTCCGGCCCCGCCTACGTACATCTACGTAAGGGACCCCCCTTAAGATAACGGCCCAAATGTCCT
TAACGGGAGGTGCCGTGTAC

Product: putative two component response regulator receiver

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSALAKPKVYVADDDADVLGSLRFLLEADGFDVRTFRSGNALLKASDRAAVDCFVVDYKMPELNGVEVADRLRKQGATAP
VVLITGHYDDRLAARAAAVGIQDFLLKPLLDDNLVKRIRKAIANDEPVTAP

Sequences:

>Translated_131_residues
MSALAKPKVYVADDDADVLGSLRFLLEADGFDVRTFRSGNALLKASDRAAVDCFVVDYKMPELNGVEVADRLRKQGATAP
VVLITGHYDDRLAARAAAVGIQDFLLKPLLDDNLVKRIRKAIANDEPVTAP
>Mature_130_residues
SALAKPKVYVADDDADVLGSLRFLLEADGFDVRTFRSGNALLKASDRAAVDCFVVDYKMPELNGVEVADRLRKQGATAPV
VLITGHYDDRLAARAAAVGIQDFLLKPLLDDNLVKRIRKAIANDEPVTAP

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=123, Percent_Identity=28.4552845528455, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 14211; Mature: 14080

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALAKPKVYVADDDADVLGSLRFLLEADGFDVRTFRSGNALLKASDRAAVDCFVVDYKM
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEEEEEEC
PELNGVEVADRLRKQGATAPVVLITGHYDDRLAARAAAVGIQDFLLKPLLDDNLVKRIRK
CCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AIANDEPVTAP
HHCCCCCCCCC
>Mature Secondary Structure 
SALAKPKVYVADDDADVLGSLRFLLEADGFDVRTFRSGNALLKASDRAAVDCFVVDYKM
CCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCEEEEEEEEEEC
PELNGVEVADRLRKQGATAPVVLITGHYDDRLAARAAAVGIQDFLLKPLLDDNLVKRIRK
CCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AIANDEPVTAP
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]