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The map label for this gene is oprM [H]

Identifier: 146339400

GI number: 146339400

Start: 2540088

End: 2541668

Strand: Reverse

Name: oprM [H]

Synonym: BRADO2385

Alternate gene names: 146339400

Gene position: 2541668-2540088 (Counterclockwise)

Preceding gene: 146339401

Following gene: 146339399

Centisome position: 34.09

GC content: 67.3

Gene sequence:

>1581_bases
ATGTCTGATCATGTCGAAATGTTTCACGCCGGCTTGCGGTGGGGCGCGGTGCGGCGCGCGACGTTGCTGCCCATCAGCCT
GATCGGTTGCGGCGTCCTGCTTGCATCATGCGCCGTCGGCCCGGATTTCCTGTCACCCGATGCGCCCGCATCGGCCGGCT
ACGTCCGCGAGCAGCGCCTCGCTGCAACCGCCGGCGCCGCAACGCTGGGCGGTGATGCGCAGAGCTTCGCAGCAGGCGGC
GACCTGCCTGCCGTCTGGTGGTCGCTGTTTCACTCGCGGCAGATCAACGCTTTCGTCACCGAGGCGATTCGCAATCATCC
GGACATCGAGGCCGCGCAATTGGCGCTGCGCGCAGCGCGCGAGAACGCGCTGTCCCAACAGGGCGCGCTGTTTCCGCAGG
TGAGCGCGAGTGGATCGGCCGAGCGCACCCAGGGATCCACCACCCAGCAGGGATATTGGCCGGGTCAGACGTCGCTGTAT
ACGCTCTATGGCGCCTCGGTCAGCGTCAGCTATGCGCTGGATATCTGGGGCGGCACACGGCGTCAGGTCGAGGCGCTGCA
GGCGCAGGCCGACTACCAGGCCTTCCGGATGGAGGCGACGTATCTGTCGCTCACCGCCAATGTCGTGACGGCCGCGATCA
CCGATGCCTCGCTGCGCGACCAAATCGCGGCGACCGAGGAGATCATCAAGGCCGAGACCGATCAGCTCGGCCGCATCCAG
CGCCAGTTCGACGTCGGCGCCATCTCCAGATCCGACGTGCTGTCGCAGGAGGCGACCTTGGCGCAGGCCAAGGCCACGCT
CGCTCCGTTGCAGAAACAATTGGCGCAGCAGCGCAACCAGCTGATGGCCTATCTCGGCCGGCTGCCGTCTCAGGACCGCG
GCGAGCATGTGACGCTTGCGGAGCTGAGACTGCCGCGCAAGCTGCCGGTCTCCCTGCCCTCCTCGCTGGTGCGCCAGCGT
CCCGACATCCGCCAGGCCGAGGCGACCCTGCACCAGGCCACCGCGACGGTGGGCGTGGGCATCGCCAATCTGCTGCCGCA
GCTCACCTTGTCGGGCAGCTATGGCGCGAGCGGGCCGGAGCGGCTGTTCTCGCCGACGACCATCGTGTGGGAGGCGGCCT
CCGGCGTCAGCCAGAAGCTGATCGACGGCGGCGCGCTGTATCACACCAAGGAGGCAGACGTTGCGACCTTCGAGCAGGAT
CTGTCGCTCTACAAAAGCACCGTGATCACCGCCTTCCAGAATGTCGCCGACTCGCTGCGCGCCATCCAATACGACGCCGA
ATCCCTGAAGGCGCAGGCGGCCGCCGAGAAGGCTACCGCCGACAGCCTCGCGATGGCGATCGAGCAATTCAAGAGCGGCG
CCGTGCCCTACGCCAACGTCATCACGGCGCAGCAATCCTATCTCAACGCCCGCGTCTCCCGCATCAAGTCGCAGGCGACG
CGCTTCACCGACACCGCCGCCCTGTTCCAGGCGCTCGGCGGCGGCTGGTGGAACCGCCAGGACGAGACACCGGCGGCGAT
GCCGCGCGCCAACCCCGGCTATTTCGCCGGCCCTACTCGCACGATCCAGGAAGCAATGTAA

Upstream 100 bases:

>100_bases
ACAACAGAGATCGCGCAGCATCGGCCCTGTCATCAACGTGACTTGACGACTCCCGGAGCGGCGAGCATCGTCCAGGCACA
AAACGAAACGGTTTAGTTTT

Downstream 100 bases:

>100_bases
TGAAGAAGCGCATGGTCATCATGCTGGCCGCGATGGCCGTTCTGTTCGGCGGCCTCTACGGTTTCCAGACGTTCAAGGGC
ATCATGATCGCCAAGGCGAT

Product: putative outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSDHVEMFHAGLRWGAVRRATLLPISLIGCGVLLASCAVGPDFLSPDAPASAGYVREQRLAATAGAATLGGDAQSFAAGG
DLPAVWWSLFHSRQINAFVTEAIRNHPDIEAAQLALRAARENALSQQGALFPQVSASGSAERTQGSTTQQGYWPGQTSLY
TLYGASVSVSYALDIWGGTRRQVEALQAQADYQAFRMEATYLSLTANVVTAAITDASLRDQIAATEEIIKAETDQLGRIQ
RQFDVGAISRSDVLSQEATLAQAKATLAPLQKQLAQQRNQLMAYLGRLPSQDRGEHVTLAELRLPRKLPVSLPSSLVRQR
PDIRQAEATLHQATATVGVGIANLLPQLTLSGSYGASGPERLFSPTTIVWEAASGVSQKLIDGGALYHTKEADVATFEQD
LSLYKSTVITAFQNVADSLRAIQYDAESLKAQAAAEKATADSLAMAIEQFKSGAVPYANVITAQQSYLNARVSRIKSQAT
RFTDTAALFQALGGGWWNRQDETPAAMPRANPGYFAGPTRTIQEAM

Sequences:

>Translated_526_residues
MSDHVEMFHAGLRWGAVRRATLLPISLIGCGVLLASCAVGPDFLSPDAPASAGYVREQRLAATAGAATLGGDAQSFAAGG
DLPAVWWSLFHSRQINAFVTEAIRNHPDIEAAQLALRAARENALSQQGALFPQVSASGSAERTQGSTTQQGYWPGQTSLY
TLYGASVSVSYALDIWGGTRRQVEALQAQADYQAFRMEATYLSLTANVVTAAITDASLRDQIAATEEIIKAETDQLGRIQ
RQFDVGAISRSDVLSQEATLAQAKATLAPLQKQLAQQRNQLMAYLGRLPSQDRGEHVTLAELRLPRKLPVSLPSSLVRQR
PDIRQAEATLHQATATVGVGIANLLPQLTLSGSYGASGPERLFSPTTIVWEAASGVSQKLIDGGALYHTKEADVATFEQD
LSLYKSTVITAFQNVADSLRAIQYDAESLKAQAAAEKATADSLAMAIEQFKSGAVPYANVITAQQSYLNARVSRIKSQAT
RFTDTAALFQALGGGWWNRQDETPAAMPRANPGYFAGPTRTIQEAM
>Mature_525_residues
SDHVEMFHAGLRWGAVRRATLLPISLIGCGVLLASCAVGPDFLSPDAPASAGYVREQRLAATAGAATLGGDAQSFAAGGD
LPAVWWSLFHSRQINAFVTEAIRNHPDIEAAQLALRAARENALSQQGALFPQVSASGSAERTQGSTTQQGYWPGQTSLYT
LYGASVSVSYALDIWGGTRRQVEALQAQADYQAFRMEATYLSLTANVVTAAITDASLRDQIAATEEIIKAETDQLGRIQR
QFDVGAISRSDVLSQEATLAQAKATLAPLQKQLAQQRNQLMAYLGRLPSQDRGEHVTLAELRLPRKLPVSLPSSLVRQRP
DIRQAEATLHQATATVGVGIANLLPQLTLSGSYGASGPERLFSPTTIVWEAASGVSQKLIDGGALYHTKEADVATFEQDL
SLYKSTVITAFQNVADSLRAIQYDAESLKAQAAAEKATADSLAMAIEQFKSGAVPYANVITAQQSYLNARVSRIKSQATR
FTDTAALFQALGGGWWNRQDETPAAMPRANPGYFAGPTRTIQEAM

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=490, Percent_Identity=25.5102040816327, Blast_Score=122, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 56328; Mature: 56197

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHVEMFHAGLRWGAVRRATLLPISLIGCGVLLASCAVGPDFLSPDAPASAGYVREQRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
AATAGAATLGGDAQSFAAGGDLPAVWWSLFHSRQINAFVTEAIRNHPDIEAAQLALRAAR
HHHHCCHHCCCCHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
ENALSQQGALFPQVSASGSAERTQGSTTQQGYWPGQTSLYTLYGASVSVSYALDIWGGTR
HHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCH
RQVEALQAQADYQAFRMEATYLSLTANVVTAAITDASLRDQIAATEEIIKAETDQLGRIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQFDVGAISRSDVLSQEATLAQAKATLAPLQKQLAQQRNQLMAYLGRLPSQDRGEHVTLA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHH
ELRLPRKLPVSLPSSLVRQRPDIRQAEATLHQATATVGVGIANLLPQLTLSGSYGASGPE
HHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHH
RLFSPTTIVWEAASGVSQKLIDGGALYHTKEADVATFEQDLSLYKSTVITAFQNVADSLR
HHCCCCCEEEECCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQYDAESLKAQAAAEKATADSLAMAIEQFKSGAVPYANVITAQQSYLNARVSRIKSQAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RFTDTAALFQALGGGWWNRQDETPAAMPRANPGYFAGPTRTIQEAM
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
SDHVEMFHAGLRWGAVRRATLLPISLIGCGVLLASCAVGPDFLSPDAPASAGYVREQRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHH
AATAGAATLGGDAQSFAAGGDLPAVWWSLFHSRQINAFVTEAIRNHPDIEAAQLALRAAR
HHHHCCHHCCCCHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHH
ENALSQQGALFPQVSASGSAERTQGSTTQQGYWPGQTSLYTLYGASVSVSYALDIWGGTR
HHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCH
RQVEALQAQADYQAFRMEATYLSLTANVVTAAITDASLRDQIAATEEIIKAETDQLGRIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RQFDVGAISRSDVLSQEATLAQAKATLAPLQKQLAQQRNQLMAYLGRLPSQDRGEHVTLA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHH
ELRLPRKLPVSLPSSLVRQRPDIRQAEATLHQATATVGVGIANLLPQLTLSGSYGASGPE
HHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHH
RLFSPTTIVWEAASGVSQKLIDGGALYHTKEADVATFEQDLSLYKSTVITAFQNVADSLR
HHCCCCCEEEECCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIQYDAESLKAQAAAEKATADSLAMAIEQFKSGAVPYANVITAQQSYLNARVSRIKSQAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RFTDTAALFQALGGGWWNRQDETPAAMPRANPGYFAGPTRTIQEAM
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]