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The map label for this gene is ligB [H]

Identifier: 146339361

GI number: 146339361

Start: 2494506

End: 2495369

Strand: Direct

Name: ligB [H]

Synonym: BRADO2342

Alternate gene names: 146339361

Gene position: 2494506-2495369 (Clockwise)

Preceding gene: 146339360

Following gene: 146339362

Centisome position: 33.45

GC content: 64.24

Gene sequence:

>864_bases
ATGGCAAAAATCTCCGCAAGCGTTTTCACCTCGCACGTTCCGGCGATCGGCGCCGCCCTCGACCTCGGCAAGACCGGCGA
GGATTATTGGAAGCCGGTGTTCGCCGGCTATGACTTCGCCAAGCAGTGGGAGAAGGAGCAGAAGCCGGATGTCGTGTTCC
TGGTCTTCAACGACCACGCGACCGCCTTCAGCCTGGATATGATCCCGACGTTCGCCATCGGCACCGCGGCCGAGTATCAG
CCGGCCGACGAGGGCTGGGGCCCGCGCCCGGTGCCCAAGGTGATCGGGCATCCGAAGCTCGCCGCGCATATCGCGCAGTC
GGTGATCCAGGACGATTTCGACCTCACCATCGTCAACAAGATGGACGTCGACCACGGCCTCACCGTGCCGCTCAGCCTGA
TCTTCGGCCAGGTCGATGCTTGGCCGTGTCCGGTGATCCCGTTCGCCGTCAACGTCGTGCAGTATCCGGTGCCGTCGGGC
CGCCGCTGCTTCATGCTGGGCAAGGCGATCCGCAAGGCGATCGAGAGCTATGACGAGCCGCTCAACGTGCAGATCTGGGG
CACCGGCGGCATGAGCCACCAGTTGCAGGGTCCGCGCGCCGGCCTGATCAACCGCGAATGGGACAACGCCTTCCTGGACA
AGCTGATCAACGATCCGGAAGGGCTGTCCAAGGTGCCGCATATCGACTACGTCCGCGAGGCCGGCAGCGAGGGCATCGAG
CTCGTGATGTGGCTGATCGCGCGCGGCGCCATGAGCGACGTCAACGAGACCAAGAAGGGCAACGCGCCGACCGTGCGTCA
CCGCTTCTACCATGTGCCGGCCAGCAACACCGCGGTCGGCCATGTGATCCTGGAGAACAACTAA

Upstream 100 bases:

>100_bases
CGGCAGCATGACCGGCATGACCGAAGAGCAATATCGCGAGATGATGATCAAGGGCGGCCGCTCGCCCGAGGGCAACCGCT
ACACCGGGGAGAAGAAGTAA

Downstream 100 bases:

>100_bases
ATGGCCAACACGATCAAGGTCGCGCTCGCCGGCGCCGGCGCCTTCGGCATCAAGCATCTCGACGGCATCAAGAACATCGA
CGGCGTCGAGGTCGTGTCGC

Product: protocatechuate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAKISASVFTSHVPAIGAALDLGKTGEDYWKPVFAGYDFAKQWEKEQKPDVVFLVFNDHATAFSLDMIPTFAIGTAAEYQ
PADEGWGPRPVPKVIGHPKLAAHIAQSVIQDDFDLTIVNKMDVDHGLTVPLSLIFGQVDAWPCPVIPFAVNVVQYPVPSG
RRCFMLGKAIRKAIESYDEPLNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDKLINDPEGLSKVPHIDYVREAGSEGIE
LVMWLIARGAMSDVNETKKGNAPTVRHRFYHVPASNTAVGHVILENN

Sequences:

>Translated_287_residues
MAKISASVFTSHVPAIGAALDLGKTGEDYWKPVFAGYDFAKQWEKEQKPDVVFLVFNDHATAFSLDMIPTFAIGTAAEYQ
PADEGWGPRPVPKVIGHPKLAAHIAQSVIQDDFDLTIVNKMDVDHGLTVPLSLIFGQVDAWPCPVIPFAVNVVQYPVPSG
RRCFMLGKAIRKAIESYDEPLNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDKLINDPEGLSKVPHIDYVREAGSEGIE
LVMWLIARGAMSDVNETKKGNAPTVRHRFYHVPASNTAVGHVILENN
>Mature_286_residues
AKISASVFTSHVPAIGAALDLGKTGEDYWKPVFAGYDFAKQWEKEQKPDVVFLVFNDHATAFSLDMIPTFAIGTAAEYQP
ADEGWGPRPVPKVIGHPKLAAHIAQSVIQDDFDLTIVNKMDVDHGLTVPLSLIFGQVDAWPCPVIPFAVNVVQYPVPSGR
RCFMLGKAIRKAIESYDEPLNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDKLINDPEGLSKVPHIDYVREAGSEGIEL
VMWLIARGAMSDVNETKKGNAPTVRHRFYHVPASNTAVGHVILENN

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=149, Percent_Identity=34.8993288590604, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 31565; Mature: 31434

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKISASVFTSHVPAIGAALDLGKTGEDYWKPVFAGYDFAKQWEKEQKPDVVFLVFNDHA
CCCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCC
TAFSLDMIPTFAIGTAAEYQPADEGWGPRPVPKVIGHPKLAAHIAQSVIQDDFDLTIVNK
CEEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
MDVDHGLTVPLSLIFGQVDAWPCPVIPFAVNVVQYPVPSGRRCFMLGKAIRKAIESYDEP
CCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCC
LNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDKLINDPEGLSKVPHIDYVREAGSEGIE
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCHHHHHHCCCCHHH
LVMWLIARGAMSDVNETKKGNAPTVRHRFYHVPASNTAVGHVILENN
HHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEECC
>Mature Secondary Structure 
AKISASVFTSHVPAIGAALDLGKTGEDYWKPVFAGYDFAKQWEKEQKPDVVFLVFNDHA
CCCCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCC
TAFSLDMIPTFAIGTAAEYQPADEGWGPRPVPKVIGHPKLAAHIAQSVIQDDFDLTIVNK
CEEEECCCCHHHCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
MDVDHGLTVPLSLIFGQVDAWPCPVIPFAVNVVQYPVPSGRRCFMLGKAIRKAIESYDEP
CCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCC
LNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDKLINDPEGLSKVPHIDYVREAGSEGIE
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCHHHHHHCCCCHHH
LVMWLIARGAMSDVNETKKGNAPTVRHRFYHVPASNTAVGHVILENN
HHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]