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The map label for this gene is ligR [H]

Identifier: 146339354

GI number: 146339354

Start: 2487884

End: 2488834

Strand: Direct

Name: ligR [H]

Synonym: BRADO2335

Alternate gene names: 146339354

Gene position: 2487884-2488834 (Clockwise)

Preceding gene: 146339353

Following gene: 146339355

Centisome position: 33.36

GC content: 70.03

Gene sequence:

>951_bases
GTGGGGCTGGCAGCGGGGCAGTCTTACGCTGGATGGCCGGGGCGTTCGTCCATGAACCTCAAGCAGCTCGAATATTTCGT
GCAGGTGGCCGAGCTCGGCTCGTTCAGCAAGGCGGCAAGCGTGCTCGACGTCGCCCAGCCGGCGCTGTCGCGGCAGGTCC
GGGCGCTGGAATCGGAGTTGAAGCAGCAGCTGTTCGCGCGCAATGGCCGCGGCGTCGAGCTCACCGAGGCCGGCAAGCGG
CTGCTCGACCACAGCGTCGGCGTGCTGCAACTGGTGGCCCATGCGCGCGAGGATCTCGGCGCCAGCCGTGACGCGCCGGT
CGGCCGCGTCACCATCGGCCTGCCGCCGAGCATCGCGCGGCAGTTGACACTGCCGCTGATCGATCGCTTCCGGAAGGAGT
ATCCGGCAGCGCGGCTCGCCGTCGTCGAGGGCCTGTCGACGCATGTGGTGGAGTGGGTCACCACGGGACGCATCGATGTC
GGCATGGTCTACAATCCCGAGGCCCAGGCCGGCATCGAGATCACGCCGGTGCTGCAGGAGCCGCTGGCGCTGGTCAGCCA
CGCGCCGAAGGGCAAGCGCCGCGTCACCGCGCCGCTGCCGATGACCGAGCTGTCGCGCTACAGCCTGATCATGCCGGAGC
GCGTGCACGCGATGCGGCGGCTGCTGGAGACGCAGGCGGCGCTGGCCGGCATCAGGCTCGACATCGCCTGGGAGGTGTCG
AGCGTGCCCTCGATCATCGATCTGGTCTGCGCCGGCTACGGCCACGCCGTGCTGACCCCGAGCGGCGTCGCAGCGTCCGG
CCGCGCCAGCGAGTTGACGATCCGGCCGCTGACCGAACCGGCCCCGACCAGCGTGCTGTGCTTCGTGACGTCAGCGCACA
AGCGGCCGACGCCACTGGCGCAGCAGACGATCAAGCTGGCGACGGCGCTGATCAGGAAACTGCCGCGCTAG

Upstream 100 bases:

>100_bases
CGACGGCTGATTGTTGGAAAAGATTCGGAGTCGCCGTTCGCGCGCAACAATCACGGCCGGTGGTTATGGGTCCCTGCGTT
CGCAGGGACGACAGCTGGGG

Downstream 100 bases:

>100_bases
CGAGCGGGGCGAGGTGCACCGAACAAGCCGCACGAGGCTACGTTCCACGTCTTCCAGACCCATTCTAAATTCAGCATGGC
TCATCTGATCTCTTATGCCG

Product: protochatechuate degradation transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MGLAAGQSYAGWPGRSSMNLKQLEYFVQVAELGSFSKAASVLDVAQPALSRQVRALESELKQQLFARNGRGVELTEAGKR
LLDHSVGVLQLVAHAREDLGASRDAPVGRVTIGLPPSIARQLTLPLIDRFRKEYPAARLAVVEGLSTHVVEWVTTGRIDV
GMVYNPEAQAGIEITPVLQEPLALVSHAPKGKRRVTAPLPMTELSRYSLIMPERVHAMRRLLETQAALAGIRLDIAWEVS
SVPSIIDLVCAGYGHAVLTPSGVAASGRASELTIRPLTEPAPTSVLCFVTSAHKRPTPLAQQTIKLATALIRKLPR

Sequences:

>Translated_316_residues
MGLAAGQSYAGWPGRSSMNLKQLEYFVQVAELGSFSKAASVLDVAQPALSRQVRALESELKQQLFARNGRGVELTEAGKR
LLDHSVGVLQLVAHAREDLGASRDAPVGRVTIGLPPSIARQLTLPLIDRFRKEYPAARLAVVEGLSTHVVEWVTTGRIDV
GMVYNPEAQAGIEITPVLQEPLALVSHAPKGKRRVTAPLPMTELSRYSLIMPERVHAMRRLLETQAALAGIRLDIAWEVS
SVPSIIDLVCAGYGHAVLTPSGVAASGRASELTIRPLTEPAPTSVLCFVTSAHKRPTPLAQQTIKLATALIRKLPR
>Mature_315_residues
GLAAGQSYAGWPGRSSMNLKQLEYFVQVAELGSFSKAASVLDVAQPALSRQVRALESELKQQLFARNGRGVELTEAGKRL
LDHSVGVLQLVAHAREDLGASRDAPVGRVTIGLPPSIARQLTLPLIDRFRKEYPAARLAVVEGLSTHVVEWVTTGRIDVG
MVYNPEAQAGIEITPVLQEPLALVSHAPKGKRRVTAPLPMTELSRYSLIMPERVHAMRRLLETQAALAGIRLDIAWEVSS
VPSIIDLVCAGYGHAVLTPSGVAASGRASELTIRPLTEPAPTSVLCFVTSAHKRPTPLAQQTIKLATALIRKLPR

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=297, Percent_Identity=29.96632996633, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI145693105, Length=297, Percent_Identity=30.976430976431, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI157672245, Length=231, Percent_Identity=29.8701298701299, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=29.2929292929293, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787601, Length=291, Percent_Identity=26.1168384879725, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787879, Length=312, Percent_Identity=27.8846153846154, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=26.8595041322314, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=275, Percent_Identity=26.9090909090909, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790208, Length=172, Percent_Identity=32.5581395348837, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787806, Length=282, Percent_Identity=28.0141843971631, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786401, Length=142, Percent_Identity=33.8028169014084, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081904, Length=170, Percent_Identity=30, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789506, Length=289, Percent_Identity=23.1833910034602, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789639, Length=126, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=275, Percent_Identity=26.1818181818182, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789204, Length=275, Percent_Identity=26.9090909090909, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788481, Length=249, Percent_Identity=27.3092369477912, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=195, Percent_Identity=28.2051282051282, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367136, Length=294, Percent_Identity=25.1700680272109, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787589, Length=272, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=126, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=252, Percent_Identity=26.984126984127, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34070; Mature: 33939

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLAAGQSYAGWPGRSSMNLKQLEYFVQVAELGSFSKAASVLDVAQPALSRQVRALESEL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQQLFARNGRGVELTEAGKRLLDHSVGVLQLVAHAREDLGASRDAPVGRVTIGLPPSIAR
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHH
QLTLPLIDRFRKEYPAARLAVVEGLSTHVVEWVTTGRIDVGMVYNPEAQAGIEITPVLQE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEHHHHH
PLALVSHAPKGKRRVTAPLPMTELSRYSLIMPERVHAMRRLLETQAALAGIRLDIAWEVS
HHHHHHCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEHH
SVPSIIDLVCAGYGHAVLTPSGVAASGRASELTIRPLTEPAPTSVLCFVTSAHKRPTPLA
CCHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHH
QQTIKLATALIRKLPR
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GLAAGQSYAGWPGRSSMNLKQLEYFVQVAELGSFSKAASVLDVAQPALSRQVRALESEL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQQLFARNGRGVELTEAGKRLLDHSVGVLQLVAHAREDLGASRDAPVGRVTIGLPPSIAR
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHH
QLTLPLIDRFRKEYPAARLAVVEGLSTHVVEWVTTGRIDVGMVYNPEAQAGIEITPVLQE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEHHHHH
PLALVSHAPKGKRRVTAPLPMTELSRYSLIMPERVHAMRRLLETQAALAGIRLDIAWEVS
HHHHHHCCCCCCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEHH
SVPSIIDLVCAGYGHAVLTPSGVAASGRASELTIRPLTEPAPTSVLCFVTSAHKRPTPLA
CCHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHH
QQTIKLATALIRKLPR
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]