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The map label for this gene is dctD [H]

Identifier: 146339325

GI number: 146339325

Start: 2455662

End: 2457020

Strand: Direct

Name: dctD [H]

Synonym: BRADO2304

Alternate gene names: 146339325

Gene position: 2455662-2457020 (Clockwise)

Preceding gene: 146339324

Following gene: 146339326

Centisome position: 32.93

GC content: 67.84

Gene sequence:

>1359_bases
ATGAGTTCTGAACAGCCGATCGGCGTGATCTATGTCGAGGACGACGAGGACGTGCGCATCGGCGCCGTGCAGGCGCTGCA
GCTCGCCGGCTTCGCGGTGACGCCGTTCGCCTCAGTCGAAGCGGCGAGCGCGGTCGTGCGTGCCGACATGCCGTTCGTGG
TTGTGTCCGACGTGCAGCTGCGCGGCAAGAGCGGCACGGCATGGCTCTCCGAGTTGCACCGGCTCGACCCGGACCTGCCG
GTGATCCTGGTCACCGGCCATGGCGACATCTCGATGGCCGTGGAGGCGATGCGCCACGGCGCCTATGACTTCATCGAGAA
GCCGTGCTCGTCCGAGCAGCTGGTGTCCGTCGTGCGCCGCGCGCTCGACAAGCGCAGGCTGACGCTGGAGGTGCGCACCC
TCCGCTCGGCGCTCGCCGACCGCCAAGGCATCGAGGCCAGCCTGCTCGGGCGCTCGCCGCAGATCCAGGAGGTGCGTCGC
ATCGTCGCCAATCTCGCCTCGACCAATGTCGACGTCACGATCTATGGCGAGACCGGCACCGGCAAGGACGTCGTCGCGCG
CTGCCTGCATGCGCATAGTGCCCGGCGCGGCGGCAACTACGTCGCGGTGAATTGCGGCGGCCTGCCGGAATCCTTGGTCG
AGAGCGAATTGTTCGGCCATGAGGCCGGCGCCTTCACCGGCGCCACCAAGCAGCGCATCGGCAAGATCGAATACGCCCAT
GGCGGCACGCTGTTCCTCGACGAGATCGAGAGCATGCCGCTCAGCGTCCAGGTCAAGCTGCTGCGCGCGCTGCAGGACCG
CGCGATCGAGCGCGTCGGCTCCAACAAGCCGGTCGCGGTGGAGTGCCGCGTCGTCGCCGCCAGCAAGGCCAATCTCTATG
AGCTCAGCGAGAGCAGGCAGTTCCGCGCCGATCTCTACTACCGCCTCGGCGTCGCCTTCATCGAGCTGCCGCCCCTGCGC
GAGCGGCGCGAGGACATTCCGATCCTGTTCGAGCATTTCACGCTCGACGCCGCCAAGCGCTTCGAGCGCGACGCGCCAAT
CGTCGACGATGCGACGATGTCGTTGCTGCTCGGCTATGCCTGGCCCGGCAACGTCCGCGAGCTGCGCAACGTCGCCGACC
GCTTCGTGCTCGGCGTGCTCGACCGGAGGGTGCTGAGCCGGTCCGCTTCCCCATCCTCTGAGCTGTCGCTGCCGCGGCAA
TTGGAGAACGTCGAACGCTCGATCATCGAGGACGCGCTGCGCCGCAAGCTCGGCGACGTGCCGGCGACGGCGGCCATGCT
CGGCGTGCCGAAGCAGACCTTGTACGACAAGATCAAGCGGCTCGCGATCGACGTCGATGGCATCCGCGAGCGCCACTGA

Upstream 100 bases:

>100_bases
CGCGCGCTCGTTCGGCGGCGAGCTGCGGGCCCGCAATCTCGAGCCCATCGGGGCCGAGTTCGTCGTGCTGCTGCCGCTTG
CCGCGTGAGAAGGTCCGCTC

Downstream 100 bases:

>100_bases
CGCGGCTCCCCGGCGTGACGGCGAGGAACGAACGCTGCGGCAGCGCCGTTGACGGCCCATGACAGGCAGAGACACCCGCG
ACCTGCGCTCCGGCGCCTAC

Product: C4-dicarboxylate transport transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSSEQPIGVIYVEDDEDVRIGAVQALQLAGFAVTPFASVEAASAVVRADMPFVVVSDVQLRGKSGTAWLSELHRLDPDLP
VILVTGHGDISMAVEAMRHGAYDFIEKPCSSEQLVSVVRRALDKRRLTLEVRTLRSALADRQGIEASLLGRSPQIQEVRR
IVANLASTNVDVTIYGETGTGKDVVARCLHAHSARRGGNYVAVNCGGLPESLVESELFGHEAGAFTGATKQRIGKIEYAH
GGTLFLDEIESMPLSVQVKLLRALQDRAIERVGSNKPVAVECRVVAASKANLYELSESRQFRADLYYRLGVAFIELPPLR
ERREDIPILFEHFTLDAAKRFERDAPIVDDATMSLLLGYAWPGNVRELRNVADRFVLGVLDRRVLSRSASPSSELSLPRQ
LENVERSIIEDALRRKLGDVPATAAMLGVPKQTLYDKIKRLAIDVDGIRERH

Sequences:

>Translated_452_residues
MSSEQPIGVIYVEDDEDVRIGAVQALQLAGFAVTPFASVEAASAVVRADMPFVVVSDVQLRGKSGTAWLSELHRLDPDLP
VILVTGHGDISMAVEAMRHGAYDFIEKPCSSEQLVSVVRRALDKRRLTLEVRTLRSALADRQGIEASLLGRSPQIQEVRR
IVANLASTNVDVTIYGETGTGKDVVARCLHAHSARRGGNYVAVNCGGLPESLVESELFGHEAGAFTGATKQRIGKIEYAH
GGTLFLDEIESMPLSVQVKLLRALQDRAIERVGSNKPVAVECRVVAASKANLYELSESRQFRADLYYRLGVAFIELPPLR
ERREDIPILFEHFTLDAAKRFERDAPIVDDATMSLLLGYAWPGNVRELRNVADRFVLGVLDRRVLSRSASPSSELSLPRQ
LENVERSIIEDALRRKLGDVPATAAMLGVPKQTLYDKIKRLAIDVDGIRERH
>Mature_451_residues
SSEQPIGVIYVEDDEDVRIGAVQALQLAGFAVTPFASVEAASAVVRADMPFVVVSDVQLRGKSGTAWLSELHRLDPDLPV
ILVTGHGDISMAVEAMRHGAYDFIEKPCSSEQLVSVVRRALDKRRLTLEVRTLRSALADRQGIEASLLGRSPQIQEVRRI
VANLASTNVDVTIYGETGTGKDVVARCLHAHSARRGGNYVAVNCGGLPESLVESELFGHEAGAFTGATKQRIGKIEYAHG
GTLFLDEIESMPLSVQVKLLRALQDRAIERVGSNKPVAVECRVVAASKANLYELSESRQFRADLYYRLGVAFIELPPLRE
RREDIPILFEHFTLDAAKRFERDAPIVDDATMSLLLGYAWPGNVRELRNVADRFVLGVLDRRVLSRSASPSSELSLPRQL
ENVERSIIEDALRRKLGDVPATAAMLGVPKQTLYDKIKRLAIDVDGIRERH

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=453, Percent_Identity=38.1898454746137, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=36.3057324840764, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1790437, Length=444, Percent_Identity=38.7387387387387, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1788905, Length=455, Percent_Identity=33.6263736263736, Blast_Score=251, Evalue=8e-68,
Organism=Escherichia coli, GI87082117, Length=341, Percent_Identity=41.9354838709677, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=332, Percent_Identity=42.1686746987952, Blast_Score=234, Evalue=9e-63,
Organism=Escherichia coli, GI1786524, Length=386, Percent_Identity=39.119170984456, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=38.2911392405063, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=45.021645021645, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=36.9905956112853, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1787583, Length=243, Percent_Identity=40.3292181069959, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87081858, Length=379, Percent_Identity=29.287598944591, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=31.9502074688797, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49781; Mature: 49649

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEQPIGVIYVEDDEDVRIGAVQALQLAGFAVTPFASVEAASAVVRADMPFVVVSDVQL
CCCCCCEEEEEEECCCCCEEHHHHHHHHCCEEECCCHHHHHHHHHHHCCCCEEEEECEEE
RGKSGTAWLSELHRLDPDLPVILVTGHGDISMAVEAMRHGAYDFIEKPCSSEQLVSVVRR
ECCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHH
ALDKRRLTLEVRTLRSALADRQGIEASLLGRSPQIQEVRRIVANLASTNVDVTIYGETGT
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCC
GKDVVARCLHAHSARRGGNYVAVNCGGLPESLVESELFGHEAGAFTGATKQRIGKIEYAH
CHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCEEECC
GGTLFLDEIESMPLSVQVKLLRALQDRAIERVGSNKPVAVECRVVAASKANLYELSESRQ
CCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHH
FRADLYYRLGVAFIELPPLRERREDIPILFEHFTLDAAKRFERDAPIVDDATMSLLLGYA
HHHHHHHHHCCCEEECCCCHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
WPGNVRELRNVADRFVLGVLDRRVLSRSASPSSELSLPRQLENVERSIIEDALRRKLGDV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
PATAAMLGVPKQTLYDKIKRLAIDVDGIRERH
CHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSEQPIGVIYVEDDEDVRIGAVQALQLAGFAVTPFASVEAASAVVRADMPFVVVSDVQL
CCCCCEEEEEEECCCCCEEHHHHHHHHCCEEECCCHHHHHHHHHHHCCCCEEEEECEEE
RGKSGTAWLSELHRLDPDLPVILVTGHGDISMAVEAMRHGAYDFIEKPCSSEQLVSVVRR
ECCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHH
ALDKRRLTLEVRTLRSALADRQGIEASLLGRSPQIQEVRRIVANLASTNVDVTIYGETGT
HHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCC
GKDVVARCLHAHSARRGGNYVAVNCGGLPESLVESELFGHEAGAFTGATKQRIGKIEYAH
CHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCEEECC
GGTLFLDEIESMPLSVQVKLLRALQDRAIERVGSNKPVAVECRVVAASKANLYELSESRQ
CCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHH
FRADLYYRLGVAFIELPPLRERREDIPILFEHFTLDAAKRFERDAPIVDDATMSLLLGYA
HHHHHHHHHCCCEEECCCCHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
WPGNVRELRNVADRFVLGVLDRRVLSRSASPSSELSLPRQLENVERSIIEDALRRKLGDV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCC
PATAAMLGVPKQTLYDKIKRLAIDVDGIRERH
CHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]