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The map label for this gene is dctB [H]

Identifier: 146339324

GI number: 146339324

Start: 2453787

End: 2455649

Strand: Direct

Name: dctB [H]

Synonym: BRADO2303

Alternate gene names: 146339324

Gene position: 2453787-2455649 (Clockwise)

Preceding gene: 146339323

Following gene: 146339325

Centisome position: 32.91

GC content: 69.46

Gene sequence:

>1863_bases
GTGGACCTGCGGGCGCGATGGGCGCGGCCCGCTACGCCCCGGATTGACCTGGCACAGGCGATGACGGACCTCCCCTTCCG
GACCGGCCTCGAGGGACATCCCGACGTTGCCGCGGTGGTTCAGGCGCGCGCGGGGTGGCTCGCGCGCGGCCTGCTGGCCA
TCGTGCTGGTCGGCGCGGCGGCCTGGCTCGGCTATGCCATCGCCTTCGCCCGGGGGCTCGACCAGCGCCATGCCTCGGCC
CAGCAGCGGCTGGCGGTCGAAGCCGCGCAGCTCGACGGCTATCTCTCGCGCTTCGAATATCTGCCGTCGCTGCTCGAGAC
GTCGCCGGACGTCTTCAGGCTGCTCGGAACGCCCGATGATCGCGCGCTGCAGCAGAGCGTCAGCCTGTATTTGAAGTCGA
TCAATCTGCTGGCCGGTGCCGACAACCTCTATGTGCTGACCGTCGACGGCAACGCGATTGCCGCCGCCGATTTCGACCAG
CCGGGCACGCCCGTGGGCAGCAATCTGTCGTACCGGCCCTATATGCGCCAGGCGCTTGCGACCGGCCGCGGCGCCTTCTT
CGGCATCGGCATCACCAGCGCCCGCGCCGGCTATTATCTCTCCTACGCGGTCAAGCGCGAGGGACGCACGATCGGCGTCG
CGGTGGTCAAGGTCAATCTCGATGCGTTCGAGCGCGAATGGCGCAGCCGCGGCGCCGACGTGCTGCTGGCCGACGCCCGC
GGCGTCACCATTCTCTCCTCGCGCGACGAATGGCGCTTTCGCCCGCTGACGCCGCTCGCGGTCGATGCGCTGGAGGAGAT
CGCGACGTCGAAGCCGTATGGCAGCTCCAGCCTCTCTCCGCTCGGCTGGACCTTCATCGAACGCGCGGAGCGCGGCGGGC
GGGTGACGACCGAGCGCGGTGCGGTCTACAGCATCGACGAGCGGCCGCTTCACGAGCGGCAATGGCAGCTGATGCTGCTC
GACGACGAGGCGCCGATCCGGCGCACGGCCTCCATCATCGGCACGCTCTCGGGCCTCGCCTGCATCGTGGCGCTGCTGCT
GTTCGGGCTGCTGGCGCAGCGGCGCAACGAAATCCGGCAACGGCTGGCGAGCCAGGCCGCGCTGCAGGCCGCCAATGACA
GGCTGGAGATGCGGGTGCAGGAGCGCACCGCGGAATTGCGCGCCGCGCAGGATGAGCTCGTGCATGCCGGCAAGCTCGCC
GTGCTCGGCCAGATGTCGGCCGGCCTGGTGCACGAGATCAATCAGCCCCTGGCGGCGCTGCAGACCGCGTCCGACAATGC
CGTGCAGTTTGTCGACCGCGGCATGGTCAGCGAGGCCCGCGGCAATCTCCTCCGTATCGGTGAGCTGGTGCGCCGCCTGG
CGCGCCTCACCGGGCAGCTGCGCGTGTTCGCCTACAAATCGAACGCGCCGCTCGACGTGGTCTCGATCGAGCAGGCGGTG
TCGGAGACGCTCACGATTCTCGGCGCGCGGGTCAAGGATGGGGGCGTCGAGATGAGCATCGATCTGGCGCCGGATCTGTA
TGTCCGCGCCGATCAGATCCGGCTCGAGCAATTGCTCTGCAACATCGTCGCCAACGCGCTCGATGCCGTCGAGGGAGCCG
CGCGCCGGTCCGTCTCGATCCGCGCCGAGCGCGACGAGGGGGAGTGCCGGATCGCCATCGTCAACAGCGGTCCGGCCATT
GCCGCCGATGTGCTGCAGCGCATGTTCGAGCCGTTCGTGACGACCAAGCCGGCCGGCAAGGGCCTTGGCCTCGGCCTGGT
CATCGCCAATCATATCGCGCGCTCGTTCGGCGGCGAGCTGCGGGCCCGCAATCTCGAGCCCATCGGGGCCGAGTTCGTCG
TGCTGCTGCCGCTTGCCGCGTGA

Upstream 100 bases:

>100_bases
CCCATGGTACCGCTCCGCGCAGCTGGAGGTTGAACTCTGCATAGCGGTACCGGCGGGGATTTTGCGGTCGCAAATTCTTT
TGTAAGACCTTAGGCTTAGG

Downstream 100 bases:

>100_bases
GAAGGTCCGCTCATGAGTTCTGAACAGCCGATCGGCGTGATCTATGTCGAGGACGACGAGGACGTGCGCATCGGCGCCGT
GCAGGCGCTGCAGCTCGCCG

Product: two-component C4-dicarboxylate transport system, sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MDLRARWARPATPRIDLAQAMTDLPFRTGLEGHPDVAAVVQARAGWLARGLLAIVLVGAAAWLGYAIAFARGLDQRHASA
QQRLAVEAAQLDGYLSRFEYLPSLLETSPDVFRLLGTPDDRALQQSVSLYLKSINLLAGADNLYVLTVDGNAIAAADFDQ
PGTPVGSNLSYRPYMRQALATGRGAFFGIGITSARAGYYLSYAVKREGRTIGVAVVKVNLDAFEREWRSRGADVLLADAR
GVTILSSRDEWRFRPLTPLAVDALEEIATSKPYGSSSLSPLGWTFIERAERGGRVTTERGAVYSIDERPLHERQWQLMLL
DDEAPIRRTASIIGTLSGLACIVALLLFGLLAQRRNEIRQRLASQAALQAANDRLEMRVQERTAELRAAQDELVHAGKLA
VLGQMSAGLVHEINQPLAALQTASDNAVQFVDRGMVSEARGNLLRIGELVRRLARLTGQLRVFAYKSNAPLDVVSIEQAV
SETLTILGARVKDGGVEMSIDLAPDLYVRADQIRLEQLLCNIVANALDAVEGAARRSVSIRAERDEGECRIAIVNSGPAI
AADVLQRMFEPFVTTKPAGKGLGLGLVIANHIARSFGGELRARNLEPIGAEFVVLLPLAA

Sequences:

>Translated_620_residues
MDLRARWARPATPRIDLAQAMTDLPFRTGLEGHPDVAAVVQARAGWLARGLLAIVLVGAAAWLGYAIAFARGLDQRHASA
QQRLAVEAAQLDGYLSRFEYLPSLLETSPDVFRLLGTPDDRALQQSVSLYLKSINLLAGADNLYVLTVDGNAIAAADFDQ
PGTPVGSNLSYRPYMRQALATGRGAFFGIGITSARAGYYLSYAVKREGRTIGVAVVKVNLDAFEREWRSRGADVLLADAR
GVTILSSRDEWRFRPLTPLAVDALEEIATSKPYGSSSLSPLGWTFIERAERGGRVTTERGAVYSIDERPLHERQWQLMLL
DDEAPIRRTASIIGTLSGLACIVALLLFGLLAQRRNEIRQRLASQAALQAANDRLEMRVQERTAELRAAQDELVHAGKLA
VLGQMSAGLVHEINQPLAALQTASDNAVQFVDRGMVSEARGNLLRIGELVRRLARLTGQLRVFAYKSNAPLDVVSIEQAV
SETLTILGARVKDGGVEMSIDLAPDLYVRADQIRLEQLLCNIVANALDAVEGAARRSVSIRAERDEGECRIAIVNSGPAI
AADVLQRMFEPFVTTKPAGKGLGLGLVIANHIARSFGGELRARNLEPIGAEFVVLLPLAA
>Mature_620_residues
MDLRARWARPATPRIDLAQAMTDLPFRTGLEGHPDVAAVVQARAGWLARGLLAIVLVGAAAWLGYAIAFARGLDQRHASA
QQRLAVEAAQLDGYLSRFEYLPSLLETSPDVFRLLGTPDDRALQQSVSLYLKSINLLAGADNLYVLTVDGNAIAAADFDQ
PGTPVGSNLSYRPYMRQALATGRGAFFGIGITSARAGYYLSYAVKREGRTIGVAVVKVNLDAFEREWRSRGADVLLADAR
GVTILSSRDEWRFRPLTPLAVDALEEIATSKPYGSSSLSPLGWTFIERAERGGRVTTERGAVYSIDERPLHERQWQLMLL
DDEAPIRRTASIIGTLSGLACIVALLLFGLLAQRRNEIRQRLASQAALQAANDRLEMRVQERTAELRAAQDELVHAGKLA
VLGQMSAGLVHEINQPLAALQTASDNAVQFVDRGMVSEARGNLLRIGELVRRLARLTGQLRVFAYKSNAPLDVVSIEQAV
SETLTILGARVKDGGVEMSIDLAPDLYVRADQIRLEQLLCNIVANALDAVEGAARRSVSIRAERDEGECRIAIVNSGPAI
AADVLQRMFEPFVTTKPAGKGLGLGLVIANHIARSFGGELRARNLEPIGAEFVVLLPLAA

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=28.3261802575107, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788549, Length=239, Percent_Identity=28.4518828451883, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87081816, Length=271, Percent_Identity=27.6752767527675, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790861, Length=218, Percent_Identity=28.8990825688073, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67250; Mature: 67250

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRARWARPATPRIDLAQAMTDLPFRTGLEGHPDVAAVVQARAGWLARGLLAIVLVGAA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AWLGYAIAFARGLDQRHASAQQRLAVEAAQLDGYLSRFEYLPSLLETSPDVFRLLGTPDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCH
RALQQSVSLYLKSINLLAGADNLYVLTVDGNAIAAADFDQPGTPVGSNLSYRPYMRQALA
HHHHHHHHHHHHHHHHEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
TGRGAFFGIGITSARAGYYLSYAVKREGRTIGVAVVKVNLDAFEREWRSRGADVLLADAR
CCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCCEEEECCC
GVTILSSRDEWRFRPLTPLAVDALEEIATSKPYGSSSLSPLGWTFIERAERGGRVTTERG
CEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCC
AVYSIDERPLHERQWQLMLLDDEAPIRRTASIIGTLSGLACIVALLLFGLLAQRRNEIRQ
CEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLASQAALQAANDRLEMRVQERTAELRAAQDELVHAGKLAVLGQMSAGLVHEINQPLAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHH
QTASDNAVQFVDRGMVSEARGNLLRIGELVRRLARLTGQLRVFAYKSNAPLDVVSIEQAV
HCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEHHHHHHHH
SETLTILGARVKDGGVEMSIDLAPDLYVRADQIRLEQLLCNIVANALDAVEGAARRSVSI
HHHHHHHHCEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RAERDEGECRIAIVNSGPAIAADVLQRMFEPFVTTKPAGKGLGLGLVIANHIARSFGGEL
EEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
RARNLEPIGAEFVVLLPLAA
CCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MDLRARWARPATPRIDLAQAMTDLPFRTGLEGHPDVAAVVQARAGWLARGLLAIVLVGAA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AWLGYAIAFARGLDQRHASAQQRLAVEAAQLDGYLSRFEYLPSLLETSPDVFRLLGTPDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCH
RALQQSVSLYLKSINLLAGADNLYVLTVDGNAIAAADFDQPGTPVGSNLSYRPYMRQALA
HHHHHHHHHHHHHHHHEECCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCHHHHHHHH
TGRGAFFGIGITSARAGYYLSYAVKREGRTIGVAVVKVNLDAFEREWRSRGADVLLADAR
CCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEEEEEHHHHHHHHHHCCCCEEEECCC
GVTILSSRDEWRFRPLTPLAVDALEEIATSKPYGSSSLSPLGWTFIERAERGGRVTTERG
CEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEECCC
AVYSIDERPLHERQWQLMLLDDEAPIRRTASIIGTLSGLACIVALLLFGLLAQRRNEIRQ
CEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLASQAALQAANDRLEMRVQERTAELRAAQDELVHAGKLAVLGQMSAGLVHEINQPLAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHH
QTASDNAVQFVDRGMVSEARGNLLRIGELVRRLARLTGQLRVFAYKSNAPLDVVSIEQAV
HCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEHHHHHHHH
SETLTILGARVKDGGVEMSIDLAPDLYVRADQIRLEQLLCNIVANALDAVEGAARRSVSI
HHHHHHHHCEECCCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
RAERDEGECRIAIVNSGPAIAADVLQRMFEPFVTTKPAGKGLGLGLVIANHIARSFGGEL
EEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
RARNLEPIGAEFVVLLPLAA
CCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]