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The map label for this gene is mutT [H]

Identifier: 146339307

GI number: 146339307

Start: 2430113

End: 2430982

Strand: Direct

Name: mutT [H]

Synonym: BRADO2283

Alternate gene names: 146339307

Gene position: 2430113-2430982 (Clockwise)

Preceding gene: 146339306

Following gene: 146339308

Centisome position: 32.59

GC content: 69.66

Gene sequence:

>870_bases
ATGAGTCCGCCCTTGATCGATGTCGCCGTTGCGGTGGTTCAGGAGCCCTCCGGCCGCGTGCTGCTCGCCGAGCGGACGGC
ACGGCAGGTGGCGGCCGGCTTCTGGGAACTACCAGGCGGCAAGATCGAACCGGGCGAAGCCGCCGCAGAAGCTGCCGCGC
GCGAGCTCAGCGAGGAGATCGGCATCCACGCGACGGGGCTTCGCCCATGGCTCTGTTATGTGCACGCCTTTCCCACCAAG
CGCGTGCGCCTGCACATCCATCGTGTCGAATCCTGGCGCGGGACACCGCACGGCCGCGAAGGCCAGCGCCTCGCCTGGGT
CGATCCGGCAGCCCCTTCGGTGGCGCCAGTGCTGCCGAGCAACGGCCGCGCGATGTCTGCGCTCGGCTTGCCACGCCTGC
TGGCCTGCATCGACGTTAACGATGTTTCCGGTGCTGTGGCCGCAGCCAGGGCCGCTCGCGATCGTGGCGCCGGTTTGATC
ATCGTACGCGCACCGCAATGCGCGCCCGGCCAGCGCGTCGCCTTGGCACGACGGATCATCGCAGCGGCCGCCGATCTGCG
CGTTTTGCTCGAGGGGCCGGCATTGGAGGCCCAGCGCGCCGGGGCCCACGGTGCGTTCGTCGGCCCTGCGAACCTGCGGC
GCAGCACGGTGCGGCCCAACCTGCCGCTGTGGGGCGCGGCCTGCAGCGACAGCAACGACCTCGTGCTTGCGGCACATCTT
GGCGCCGATTTCGCGATCGCGACACCGGCGCATCCGGCGAGCAGCCGGACGCCCCATTTCACCATGTTCCGCACCGATGA
CACCGCGGCCCCGCTGCCGCTGTTCAAGACAGCTGACAAGACCATCCGCGACACTTCACGCCCCTCGTAA

Upstream 100 bases:

>100_bases
GCGGAGTGCCCGGTCCGGCCGAGATCGACTATGCGCGCCGTGCCGCGGTGCTGGCGGCCGCGTGGACCATCCGGTGAGTG
ATTGAATTCGGAGGTGTGCG

Downstream 100 bases:

>100_bases
CATGGGACTGCGAAGATGATGACGTCCTCGCTCTGGCTCGCAATATCCGGTCCTGGCGCGCTGCTTGCGGCCTCGCTGGT
GCCGGCCATCCATCCCACGA

Product: hypothetical protein

Products: diphosphate; thiamine monophosphate

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSPPLIDVAVAVVQEPSGRVLLAERTARQVAAGFWELPGGKIEPGEAAAEAAARELSEEIGIHATGLRPWLCYVHAFPTK
RVRLHIHRVESWRGTPHGREGQRLAWVDPAAPSVAPVLPSNGRAMSALGLPRLLACIDVNDVSGAVAAARAARDRGAGLI
IVRAPQCAPGQRVALARRIIAAAADLRVLLEGPALEAQRAGAHGAFVGPANLRRSTVRPNLPLWGAACSDSNDLVLAAHL
GADFAIATPAHPASSRTPHFTMFRTDDTAAPLPLFKTADKTIRDTSRPS

Sequences:

>Translated_289_residues
MSPPLIDVAVAVVQEPSGRVLLAERTARQVAAGFWELPGGKIEPGEAAAEAAARELSEEIGIHATGLRPWLCYVHAFPTK
RVRLHIHRVESWRGTPHGREGQRLAWVDPAAPSVAPVLPSNGRAMSALGLPRLLACIDVNDVSGAVAAARAARDRGAGLI
IVRAPQCAPGQRVALARRIIAAAADLRVLLEGPALEAQRAGAHGAFVGPANLRRSTVRPNLPLWGAACSDSNDLVLAAHL
GADFAIATPAHPASSRTPHFTMFRTDDTAAPLPLFKTADKTIRDTSRPS
>Mature_288_residues
SPPLIDVAVAVVQEPSGRVLLAERTARQVAAGFWELPGGKIEPGEAAAEAAARELSEEIGIHATGLRPWLCYVHAFPTKR
VRLHIHRVESWRGTPHGREGQRLAWVDPAAPSVAPVLPSNGRAMSALGLPRLLACIDVNDVSGAVAAARAARDRGAGLII
VRAPQCAPGQRVALARRIIAAAADLRVLLEGPALEAQRAGAHGAFVGPANLRRSTVRPNLPLWGAACSDSNDLVLAAHLG
ADFAIATPAHPASSRTPHFTMFRTDDTAAPLPLFKTADKTIRDTSRPS

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=117, Percent_Identity=35.8974358974359, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786288, Length=123, Percent_Identity=30.8943089430894, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 2.5.1.3; 3.6.1.-

Molecular weight: Translated: 30489; Mature: 30358

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPLIDVAVAVVQEPSGRVLLAERTARQVAAGFWELPGGKIEPGEAAAEAAARELSEEI
CCCCHHHHHEEEEECCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
GIHATGLRPWLCYVHAFPTKRVRLHIHRVESWRGTPHGREGQRLAWVDPAAPSVAPVLPS
CEEEECCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
NGRAMSALGLPRLLACIDVNDVSGAVAAARAARDRGAGLIIVRAPQCAPGQRVALARRII
CCCEEEHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
AAAADLRVLLEGPALEAQRAGAHGAFVGPANLRRSTVRPNLPLWGAACSDSNDLVLAAHL
HHHHCEEEEEECCCCHHHHCCCCCEEECCHHHHHCCCCCCCCEEEECCCCCCCEEEEEEC
GADFAIATPAHPASSRTPHFTMFRTDDTAAPLPLFKTADKTIRDTSRPS
CCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCHHHHCCCCCC
>Mature Secondary Structure 
SPPLIDVAVAVVQEPSGRVLLAERTARQVAAGFWELPGGKIEPGEAAAEAAARELSEEI
CCCHHHHHEEEEECCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
GIHATGLRPWLCYVHAFPTKRVRLHIHRVESWRGTPHGREGQRLAWVDPAAPSVAPVLPS
CEEEECCCCEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
NGRAMSALGLPRLLACIDVNDVSGAVAAARAARDRGAGLIIVRAPQCAPGQRVALARRII
CCCEEEHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
AAAADLRVLLEGPALEAQRAGAHGAFVGPANLRRSTVRPNLPLWGAACSDSNDLVLAAHL
HHHHCEEEEEECCCCHHHHCCCCCEEECCHHHHHCCCCCCCCEEEECCCCCCCEEEEEEC
GADFAIATPAHPASSRTPHFTMFRTDDTAAPLPLFKTADKTIRDTSRPS
CCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCHHHCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]