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The map label for this gene is ndhF [H]

Identifier: 146339308

GI number: 146339308

Start: 2430998

End: 2432626

Strand: Direct

Name: ndhF [H]

Synonym: BRADO2284

Alternate gene names: 146339308

Gene position: 2430998-2432626 (Clockwise)

Preceding gene: 146339307

Following gene: 146339309

Centisome position: 32.6

GC content: 64.7

Gene sequence:

>1629_bases
ATGATGACGTCCTCGCTCTGGCTCGCAATATCCGGTCCTGGCGCGCTGCTTGCGGCCTCGCTGGTGCCGGCCATCCATCC
CACGATCTCGCATCCGTCGATGAATCGCCTGGCTGGACTCGCCTCGGGTCTGGCCTGCGCGGTTGCCTTCGTCACCGCGC
TCCAGGTCGGCATCAGTGGACCGCTCTACACGCCGTCACTTGCGATCAACGGCGTCGGTCTTGGCCTTTATCTCGACAGC
GTGAGTGCCACGATGTTCTGCCTGGTGTCCTTCATCGGCGTTATCGTGCTGCGCTACAGCTTCACCTATATGGACGGCGA
TCCCGGCCAGATCCGCTTCATCCGTCTGATGTGCCTGACGCTGGCGGCGGTGCTGACGCTAGCGATATCCGGCAATCTGC
TGCAATTCACGCTGGCCTGGTCGGCGACAAGCCTGGCGCTGCATCGTCTGCTGGTGTTCTACGCCGAACGGCCGGCGGCG
GTGATTGCCGCCCGCAAGAAGTTCATCGCCAGCAGGCTCGGCGACCTGTTCCTGGTCACGGCCATGGTGCTGCTCTATGC
CGCCTTCGGCAGCCTCGACTACCAGACGATGTTCTCGGCCGCCAAGGCGATGCGGGCGCAGGAGACGGTGCCGCTCGGCA
TCCAGATCGCGGCTGTTCTGATGGTGTTTGCGGGTCTCTTGAAGTCGGCGCAATTCCCGCTGCATGGCTGGCTCATCGAG
GTGATGGAGACCCCGACGCCGGTGTCGGCGCTGCTGCATGCCGGCATCATCAATGCCGGCGGGTTCCTGGTGCTGCGCTT
CACCGACGTGGTTACGCTGTCGCTTGCGTCGCTCGACCTGCTTGCGGTGGTCGGCGGCTTCACGGCGTTGTTTGGCTCGG
TCGTGATGCTGACCCAGACGTCGATCAAGGTCTCGCTTGCCTGGTCGACGATCGCGCAGATGGGATTCATGATGCTGGAA
TGCGGTCTCGGCGCCTTCGCCGCGGCGCTGCTGCACATCGTCGCCCACTCGCTTTACAAGGCGCATGCCTTCCTGTCCTC
AGGCAGCGTCATCGACATCGCTCGCGCCTCGTGGACCCCGAGCCCGGGCGGCAAGCCGCATCCATTCCGGCTGATCATCT
CCATTATCGGCGTGCTGCTGGTGACGCTGTTGTTCAGCCTGCTGACCGGCGCGACGCCGGCCCGCCAGCCCGGCGTCTTC
GCGCTCGGCGCTGTGCTCCTGCTCGGCATGATCCACCTCGTCGCGAACGGCATCGACGAACGGCCGAACGCTTACGTCGT
GCTGCGCACGCTGCTCTTGGCAGCCGTGGTTGCCGGCGCCTATTTCGGCCTGCAACTGGTCGCGGAGCAATTGTTTGCCG
CGGCTCTGCCGAAGCTCGAAGCGATGCGCGGGCCGATCGGGATCGTGATCGTGGCGCTGGTGGTGCTGTCCTTCGCGGCT
GTGACGTTCCTGCAGGGCCTGGTGCCGAAGCAGGCGACGGAGCCACGCTGGCAGGCGCTCTACGTGCATGTTGCCAACGG
CTTCTATGTCAACACCATCGCCAATCGCCTGGTCCTGCAGTATTGGCCCGGCCCAGCGCCGAAGCCCGCTCCGATTGCTC
TTCCCATTGTGCTGAGGGAGCCCACATGA

Upstream 100 bases:

>100_bases
GTTCCGCACCGATGACACCGCGGCCCCGCTGCCGCTGTTCAAGACAGCTGACAAGACCATCCGCGACACTTCACGCCCCT
CGTAACATGGGACTGCGAAG

Downstream 100 bases:

>100_bases
GCGACGTGATCGAATGCGACGCACCGGCCGTCGCCCTTGCGCCAACGCCAGCCCTGCACGCCGCCATCGCGGGAGCCTGT
CGCCGCATCGCACCGCTGTG

Product: putative NADH dehydrogenase

Products: NAD+; ubiquinol [C]

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MMTSSLWLAISGPGALLAASLVPAIHPTISHPSMNRLAGLASGLACAVAFVTALQVGISGPLYTPSLAINGVGLGLYLDS
VSATMFCLVSFIGVIVLRYSFTYMDGDPGQIRFIRLMCLTLAAVLTLAISGNLLQFTLAWSATSLALHRLLVFYAERPAA
VIAARKKFIASRLGDLFLVTAMVLLYAAFGSLDYQTMFSAAKAMRAQETVPLGIQIAAVLMVFAGLLKSAQFPLHGWLIE
VMETPTPVSALLHAGIINAGGFLVLRFTDVVTLSLASLDLLAVVGGFTALFGSVVMLTQTSIKVSLAWSTIAQMGFMMLE
CGLGAFAAALLHIVAHSLYKAHAFLSSGSVIDIARASWTPSPGGKPHPFRLIISIIGVLLVTLLFSLLTGATPARQPGVF
ALGAVLLLGMIHLVANGIDERPNAYVVLRTLLLAAVVAGAYFGLQLVAEQLFAAALPKLEAMRGPIGIVIVALVVLSFAA
VTFLQGLVPKQATEPRWQALYVHVANGFYVNTIANRLVLQYWPGPAPKPAPIALPIVLREPT

Sequences:

>Translated_542_residues
MMTSSLWLAISGPGALLAASLVPAIHPTISHPSMNRLAGLASGLACAVAFVTALQVGISGPLYTPSLAINGVGLGLYLDS
VSATMFCLVSFIGVIVLRYSFTYMDGDPGQIRFIRLMCLTLAAVLTLAISGNLLQFTLAWSATSLALHRLLVFYAERPAA
VIAARKKFIASRLGDLFLVTAMVLLYAAFGSLDYQTMFSAAKAMRAQETVPLGIQIAAVLMVFAGLLKSAQFPLHGWLIE
VMETPTPVSALLHAGIINAGGFLVLRFTDVVTLSLASLDLLAVVGGFTALFGSVVMLTQTSIKVSLAWSTIAQMGFMMLE
CGLGAFAAALLHIVAHSLYKAHAFLSSGSVIDIARASWTPSPGGKPHPFRLIISIIGVLLVTLLFSLLTGATPARQPGVF
ALGAVLLLGMIHLVANGIDERPNAYVVLRTLLLAAVVAGAYFGLQLVAEQLFAAALPKLEAMRGPIGIVIVALVVLSFAA
VTFLQGLVPKQATEPRWQALYVHVANGFYVNTIANRLVLQYWPGPAPKPAPIALPIVLREPT
>Mature_542_residues
MMTSSLWLAISGPGALLAASLVPAIHPTISHPSMNRLAGLASGLACAVAFVTALQVGISGPLYTPSLAINGVGLGLYLDS
VSATMFCLVSFIGVIVLRYSFTYMDGDPGQIRFIRLMCLTLAAVLTLAISGNLLQFTLAWSATSLALHRLLVFYAERPAA
VIAARKKFIASRLGDLFLVTAMVLLYAAFGSLDYQTMFSAAKAMRAQETVPLGIQIAAVLMVFAGLLKSAQFPLHGWLIE
VMETPTPVSALLHAGIINAGGFLVLRFTDVVTLSLASLDLLAVVGGFTALFGSVVMLTQTSIKVSLAWSTIAQMGFMMLE
CGLGAFAAALLHIVAHSLYKAHAFLSSGSVIDIARASWTPSPGGKPHPFRLIISIIGVLLVTLLFSLLTGATPARQPGVF
ALGAVLLLGMIHLVANGIDERPNAYVVLRTLLLAAVVAGAYFGLQLVAEQLFAAALPKLEAMRGPIGIVIVALVVLSFAA
VTFLQGLVPKQATEPRWQALYVHVANGFYVNTIANRLVLQYWPGPAPKPAPIALPIVLREPT

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=281, Percent_Identity=32.0284697508897, Blast_Score=129, Evalue=9e-30,
Organism=Escherichia coli, GI1788614, Length=296, Percent_Identity=38.1756756756757, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788831, Length=300, Percent_Identity=29.6666666666667, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788829, Length=297, Percent_Identity=28.956228956229, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788827, Length=312, Percent_Identity=26.6025641025641, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 57210; Mature: 57210

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTSSLWLAISGPGALLAASLVPAIHPTISHPSMNRLAGLASGLACAVAFVTALQVGISG
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLYTPSLAINGVGLGLYLDSVSATMFCLVSFIGVIVLRYSFTYMDGDPGQIRFIRLMCLT
CCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
LAAVLTLAISGNLLQFTLAWSATSLALHRLLVFYAERPAAVIAARKKFIASRLGDLFLVT
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AMVLLYAAFGSLDYQTMFSAAKAMRAQETVPLGIQIAAVLMVFAGLLKSAQFPLHGWLIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VMETPTPVSALLHAGIINAGGFLVLRFTDVVTLSLASLDLLAVVGGFTALFGSVVMLTQT
HHCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
SIKVSLAWSTIAQMGFMMLECGLGAFAAALLHIVAHSLYKAHAFLSSGSVIDIARASWTP
CEEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
SPGGKPHPFRLIISIIGVLLVTLLFSLLTGATPARQPGVFALGAVLLLGMIHLVANGIDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
RPNAYVVLRTLLLAAVVAGAYFGLQLVAEQLFAAALPKLEAMRGPIGIVIVALVVLSFAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHH
VTFLQGLVPKQATEPRWQALYVHVANGFYVNTIANRLVLQYWPGPAPKPAPIALPIVLRE
HHHHHHCCCCCCCCCCCEEEEEEECCCEEHHHHHHHHEEEECCCCCCCCCCEEEEEEEEC
PT
CC
>Mature Secondary Structure
MMTSSLWLAISGPGALLAASLVPAIHPTISHPSMNRLAGLASGLACAVAFVTALQVGISG
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLYTPSLAINGVGLGLYLDSVSATMFCLVSFIGVIVLRYSFTYMDGDPGQIRFIRLMCLT
CCCCCCEEECCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
LAAVLTLAISGNLLQFTLAWSATSLALHRLLVFYAERPAAVIAARKKFIASRLGDLFLVT
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AMVLLYAAFGSLDYQTMFSAAKAMRAQETVPLGIQIAAVLMVFAGLLKSAQFPLHGWLIE
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VMETPTPVSALLHAGIINAGGFLVLRFTDVVTLSLASLDLLAVVGGFTALFGSVVMLTQT
HHCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
SIKVSLAWSTIAQMGFMMLECGLGAFAAALLHIVAHSLYKAHAFLSSGSVIDIARASWTP
CEEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
SPGGKPHPFRLIISIIGVLLVTLLFSLLTGATPARQPGVFALGAVLLLGMIHLVANGIDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
RPNAYVVLRTLLLAAVVAGAYFGLQLVAEQLFAAALPKLEAMRGPIGIVIVALVVLSFAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHH
VTFLQGLVPKQATEPRWQALYVHVANGFYVNTIANRLVLQYWPGPAPKPAPIALPIVLRE
HHHHHHCCCCCCCCCCCEEEEEEECCCEEHHHHHHHHEEEECCCCCCCCCCEEEEEEEEC
PT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]