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The map label for this gene is yhcR [H]

Identifier: 146339204

GI number: 146339204

Start: 2300524

End: 2302215

Strand: Reverse

Name: yhcR [H]

Synonym: BRADO2166

Alternate gene names: 146339204

Gene position: 2302215-2300524 (Counterclockwise)

Preceding gene: 146339206

Following gene: 146339203

Centisome position: 30.87

GC content: 65.9

Gene sequence:

>1692_bases
ATGCCTGACCGCACCATGAGCCGTTTCCATCGCGTCGCCGTCGCCTGTCTCTTCGCCGCTTTCGTGGCCGCGAGTCTGGC
CGCCCTGCCCGTTCATGCCGAGGACGAGCCGGTGTCGTTGCGCATTCTCGCCATCAACGATTTCCACGGCAATCTGAAGC
CGCCAAGCGCCGGCATTCGCGTCGCCGATCCGAACGATCCGACCAAGAGCACGCCGGTGCCGGCGGGCGGAGCAGCGCAG
ATGGCGAGCCTCGTCAAGCTCTTGTCCGACGGGCAGCCGAACACGATCTTCGTCGCCGCCGGCGACCTCATCGGCGCAAG
CCCATTTCTGTCGGCGATGTTCCATGACGAGCCGACGATCGAATCGATGTCGCAGATGGGGCTTGCGCTGTCGGCTGTCG
GCAATCACGAGTTCGACGAGGGCAAGGACGAGCTGTTGCGGATGCAGAATGGCGGCTGCCATCCGACCGATGGCTGCCGC
GGGCCGCACCCCTTCACCGGCGCCAAATTCCACTATCTCGCCGCCTCCACCTATCTGAAGGGCACCGACAAGACGGTGTT
TCCGCCTTACGAAATCCGCACCTTCGAGGACATCCCGGTCGCCTTCATCGGGCTGACGCTGAAGGGGACCGCCGGCATCA
TTTCGCCGGCGAGCTCGGCGGGCCTCGAATTCCGCAACGAGGCGGAGAGCATCAACGCGCTGATCCCCGAATTGAAGGCC
AAGGGGGTGCAGGCGATCGTGGTGATGATCCACGAAGGCGGCTGGCCTTCGGGCGGCATGAACGAATGCCCCGGGCTATC
CGGTCCGATCGTCGACATCGTCAAGGATCTCGATAGCGCCGTCGATATCGTCATTTCCGGCCACACGCACCAGGCCTATG
TCTGCACCATCGACAAGCGGCTCGTCTCCTCGGCCGACAAGTTCGGCACGCTCGTCACCGCGATCGACGTCAAGCTCGAC
CGCACGACGCGCGACGTGATCAGCACCAGCGCCACCAACATCGTCGTCGACGCCACGCTGCCGAAGGATCCGACGCAGAC
CGCGCTGATCGACGCCTACGAGAAGCTCGCCGCGCCGCTCGCGAACCGGCCCGCGGGCAACATCACGCAGGTGCTGTCGC
GGCTGCCGAATGCGGCCGGCGAAAGCGTGCTCGGCGATGTCATCGCCGACGCCCAGCTCGCGGCGACATCGCCGGCGGAC
AAGGGCGGCGCCGTCATCGCGATGACAAATCCCGGCGGCATCCGCACCGATCTCGCCCGGCGCGACGACGGCGCGGTCAC
CTTCGCCGATGTGTTTGCGAGCCAGCCGTTCCGCAACCAGCTGATGACGATCAGCCTGACGGGGGCCGACATCAGGGCCG
CGCTGGAGCAGCAGTGGGCCGATCCGGCGCGGCCACGAATCCTGCAAATCTCGCGCGGCTTCTCCTATGCGTTCGACGCG
AAGCAGCCGGCCGGCGCGCGCATCGATGCCGCATCGATGACGCTGAATGGCAAGCCGATCGACGCGGCGGCGCCGTACCG
CGTCACGGTCAACAACTATCTCGCGCTCGGCGGAGACGGCTTCACGGCGTTCAAGAACGGCCGGGATCCGGTTGTCGGTC
CTTATGATGACGAGGCGCTGTTCGCCTATCTCAAATCCAACAGCCCGCTCGCCCCGCCGCCCCGCGACCGCATTTCAAAC
AAGGCCCCTTGA

Upstream 100 bases:

>100_bases
CCATTCCCGGTATTGTTGTTATGAATTTGTCTTATGTCATCGCGATCGCGACGAAGCAATCGTACGTCCTCCGCTGTCCC
GCATCCTCCGCGCGCCTGCC

Downstream 100 bases:

>100_bases
CGCCCGAACGGCGGGAAAGCCAGTCAGATCCTCCGTCATGGCTCTTTCCGAGGCTGCGTTCAGCGCATAGATTGGGCACT
CCCGCAACGCGCGAGTGGCA

Product: putative 5'-nucleotidase

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MPDRTMSRFHRVAVACLFAAFVAASLAALPVHAEDEPVSLRILAINDFHGNLKPPSAGIRVADPNDPTKSTPVPAGGAAQ
MASLVKLLSDGQPNTIFVAAGDLIGASPFLSAMFHDEPTIESMSQMGLALSAVGNHEFDEGKDELLRMQNGGCHPTDGCR
GPHPFTGAKFHYLAASTYLKGTDKTVFPPYEIRTFEDIPVAFIGLTLKGTAGIISPASSAGLEFRNEAESINALIPELKA
KGVQAIVVMIHEGGWPSGGMNECPGLSGPIVDIVKDLDSAVDIVISGHTHQAYVCTIDKRLVSSADKFGTLVTAIDVKLD
RTTRDVISTSATNIVVDATLPKDPTQTALIDAYEKLAAPLANRPAGNITQVLSRLPNAAGESVLGDVIADAQLAATSPAD
KGGAVIAMTNPGGIRTDLARRDDGAVTFADVFASQPFRNQLMTISLTGADIRAALEQQWADPARPRILQISRGFSYAFDA
KQPAGARIDAASMTLNGKPIDAAAPYRVTVNNYLALGGDGFTAFKNGRDPVVGPYDDEALFAYLKSNSPLAPPPRDRISN
KAP

Sequences:

>Translated_563_residues
MPDRTMSRFHRVAVACLFAAFVAASLAALPVHAEDEPVSLRILAINDFHGNLKPPSAGIRVADPNDPTKSTPVPAGGAAQ
MASLVKLLSDGQPNTIFVAAGDLIGASPFLSAMFHDEPTIESMSQMGLALSAVGNHEFDEGKDELLRMQNGGCHPTDGCR
GPHPFTGAKFHYLAASTYLKGTDKTVFPPYEIRTFEDIPVAFIGLTLKGTAGIISPASSAGLEFRNEAESINALIPELKA
KGVQAIVVMIHEGGWPSGGMNECPGLSGPIVDIVKDLDSAVDIVISGHTHQAYVCTIDKRLVSSADKFGTLVTAIDVKLD
RTTRDVISTSATNIVVDATLPKDPTQTALIDAYEKLAAPLANRPAGNITQVLSRLPNAAGESVLGDVIADAQLAATSPAD
KGGAVIAMTNPGGIRTDLARRDDGAVTFADVFASQPFRNQLMTISLTGADIRAALEQQWADPARPRILQISRGFSYAFDA
KQPAGARIDAASMTLNGKPIDAAAPYRVTVNNYLALGGDGFTAFKNGRDPVVGPYDDEALFAYLKSNSPLAPPPRDRISN
KAP
>Mature_562_residues
PDRTMSRFHRVAVACLFAAFVAASLAALPVHAEDEPVSLRILAINDFHGNLKPPSAGIRVADPNDPTKSTPVPAGGAAQM
ASLVKLLSDGQPNTIFVAAGDLIGASPFLSAMFHDEPTIESMSQMGLALSAVGNHEFDEGKDELLRMQNGGCHPTDGCRG
PHPFTGAKFHYLAASTYLKGTDKTVFPPYEIRTFEDIPVAFIGLTLKGTAGIISPASSAGLEFRNEAESINALIPELKAK
GVQAIVVMIHEGGWPSGGMNECPGLSGPIVDIVKDLDSAVDIVISGHTHQAYVCTIDKRLVSSADKFGTLVTAIDVKLDR
TTRDVISTSATNIVVDATLPKDPTQTALIDAYEKLAAPLANRPAGNITQVLSRLPNAAGESVLGDVIADAQLAATSPADK
GGAVIAMTNPGGIRTDLARRDDGAVTFADVFASQPFRNQLMTISLTGADIRAALEQQWADPARPRILQISRGFSYAFDAK
QPAGARIDAASMTLNGKPIDAAAPYRVTVNNYLALGGDGFTAFKNGRDPVVGPYDDEALFAYLKSNSPLAPPPRDRISNK
AP

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=522, Percent_Identity=23.3716475095785, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786687, Length=591, Percent_Identity=25.8883248730964, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1790658, Length=252, Percent_Identity=26.5873015873016, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922446, Length=572, Percent_Identity=25.1748251748252, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24654424, Length=572, Percent_Identity=25.1748251748252, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI221329836, Length=581, Percent_Identity=24.2685025817556, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24652512, Length=511, Percent_Identity=25.0489236790607, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19921980, Length=511, Percent_Identity=25.0489236790607, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161076508, Length=510, Percent_Identity=24.7058823529412, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19922444, Length=602, Percent_Identity=24.2524916943522, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24641187, Length=584, Percent_Identity=22.7739726027397, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 59300; Mature: 59169

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRTMSRFHRVAVACLFAAFVAASLAALPVHAEDEPVSLRILAINDFHGNLKPPSAGIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEE
VADPNDPTKSTPVPAGGAAQMASLVKLLSDGQPNTIFVAAGDLIGASPFLSAMFHDEPTI
EECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHCCCHHHHHHHCCCCCH
ESMSQMGLALSAVGNHEFDEGKDELLRMQNGGCHPTDGCRGPHPFTGAKFHYLAASTYLK
HHHHHHCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEC
GTDKTVFPPYEIRTFEDIPVAFIGLTLKGTAGIISPASSAGLEFRNEAESINALIPELKA
CCCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
KGVQAIVVMIHEGGWPSGGMNECPGLSGPIVDIVKDLDSAVDIVISGHTHQAYVCTIDKR
CCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEEHHH
LVSSADKFGTLVTAIDVKLDRTTRDVISTSATNIVVDATLPKDPTQTALIDAYEKLAAPL
HHHCHHHHCEEEEEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
ANRPAGNITQVLSRLPNAAGESVLGDVIADAQLAATSPADKGGAVIAMTNPGGIRTDLAR
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCCCCCCEEEEEECCCCCCHHHHC
RDDGAVTFADVFASQPFRNQLMTISLTGADIRAALEQQWADPARPRILQISRGFSYAFDA
CCCCCEEEHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCEEECC
KQPAGARIDAASMTLNGKPIDAAAPYRVTVNNYLALGGDGFTAFKNGRDPVVGPYDDEAL
CCCCCCEEEEEEEEECCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHHE
FAYLKSNSPLAPPPRDRISNKAP
EEEEECCCCCCCCCHHHCCCCCC
>Mature Secondary Structure 
PDRTMSRFHRVAVACLFAAFVAASLAALPVHAEDEPVSLRILAINDFHGNLKPPSAGIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEE
VADPNDPTKSTPVPAGGAAQMASLVKLLSDGQPNTIFVAAGDLIGASPFLSAMFHDEPTI
EECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHCCCHHHHHHHCCCCCH
ESMSQMGLALSAVGNHEFDEGKDELLRMQNGGCHPTDGCRGPHPFTGAKFHYLAASTYLK
HHHHHHCEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEC
GTDKTVFPPYEIRTFEDIPVAFIGLTLKGTAGIISPASSAGLEFRNEAESINALIPELKA
CCCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
KGVQAIVVMIHEGGWPSGGMNECPGLSGPIVDIVKDLDSAVDIVISGHTHQAYVCTIDKR
CCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEEHHH
LVSSADKFGTLVTAIDVKLDRTTRDVISTSATNIVVDATLPKDPTQTALIDAYEKLAAPL
HHHCHHHHCEEEEEEEEEECCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHH
ANRPAGNITQVLSRLPNAAGESVLGDVIADAQLAATSPADKGGAVIAMTNPGGIRTDLAR
CCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCCCCCCEEEEEECCCCCCHHHHC
RDDGAVTFADVFASQPFRNQLMTISLTGADIRAALEQQWADPARPRILQISRGFSYAFDA
CCCCCEEEHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCEEECC
KQPAGARIDAASMTLNGKPIDAAAPYRVTVNNYLALGGDGFTAFKNGRDPVVGPYDDEAL
CCCCCCEEEEEEEEECCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCHHE
FAYLKSNSPLAPPPRDRISNKAP
EEEEECCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]