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The map label for this gene is alkK [H]

Identifier: 146339180

GI number: 146339180

Start: 2275542

End: 2277170

Strand: Reverse

Name: alkK [H]

Synonym: BRADO2140

Alternate gene names: 146339180

Gene position: 2277170-2275542 (Counterclockwise)

Preceding gene: 146339182

Following gene: 146339179

Centisome position: 30.54

GC content: 63.6

Gene sequence:

>1629_bases
ATGCTTGGATTGATGCAAGACTGGCCCCTGCTTTGCCACCGGATCATCGAGCATGCAGCGACAATTCACGGCTCCCAGGA
GGTCGTGACCCGCTCGGTCGAAGGTCCCATCGTTCGCACCACCTACCGGCAAATCCACGACCGCGCGCTGAAAGTGTCCC
AGAAGCTGACGCGCGACGGCATCAAGCTCGGCGACCGCGTCGCCACCATCGCCTGGAATACCGGACGCCATCTCGAGGTC
TGGTACGGCATTATGGGAATCGGCGCGATCTGTCACACGGTCAATCCGCGTCTCTTCCCGGAGCAGATCGGCTGGATCAT
CAACCACGCCCAGGACCGCATCGTCATTACCGATCTGACCTTCGTGCCGCTGCTCGAGAAGCTCGCCGACAAGCTGCCGA
GCGTCGAGCGGTATGTGGTGCTGACCGACGCGGCACACATGCCGCAGACCACGCTGAAGAACGCCGTTGCCTACGAATCG
TGGATCGGGGAGGCCGATGGCGATTTCGCCTGGGGCACGTTCGACGAAAATACCGCGGCAGCGATGTGCTACACGTCCGG
CACCACCGGCGATCCCAAGGGTGTGCTGTATTCGCATCGCTCCAACGTGCTGCATGCGTTGATGGCCAACAACCGCGACT
CACTCGGAACATCCGCGGCAGACACAATGCTTCCGGTGGTCCCGCTGTTCCATGCCAACAGCTGGGGCATCGCGTTCTCC
GCACCGTCGATGGGCACCAAGCTGGTCATGCCCGGCCCGAAGCTCGACGGCGCCTCGGTCTACGAGCTGCTCGACACCGA
GAAGGTGACCTACACCGCCGGCGTGCCGACGGTGTGGCTGATGCTGCTTCAGCACATGCAGGCGCATAGGCTGACTCTGC
CGCATCTGAAGATGGTGGTGTGCGGCGGCTCGGCGATGCCGCGCTCGATGATCCAGGCCTTCCTCGACATGGGCATCGGT
GTGCGCCACGCCTGGGGCATGACCGAGATGAGCCCGATCGGCACACTCGCGACCCTGAAGCCGCCGTTCCTCGAAGCTGA
GGGCGACGCCAAGCTCGACATCCTGCAGACCCAGGGCTTCCCGCCGTTCGGCGTCGAGATGAAGATCACCGACGATGCCG
GAGCCGAGCTGCCGTGGGACGGCAAGACCTTCGGCCGCCTCAAAGTGTCCGGTCCCGCAGTGTCGAAGGCGTACTACAAG
GTCGACACCAAGATCCTCGACGACGAAGGCTTCTTCGACACCGGCGACGTCGCCACCGTCGATGCCGATGGCTTCATGCG
CATCACCGACCGCTCCAAGGACGTCATCAAGTCGGGCGGCGAGTGGATCTCGTCGATCGATCTGGAAAACCTTGCCGTCG
GCCATCCCGCCGTCGCGGAAGCCGCCGTCATCGGCGTCTATCATCCGAAATGGGACGAGCGGCCGCTTCTGATCGTGCAG
CTCAAGCAGGGCCAGACGGCGACGCGCGAGGACATCCTGAAGTTCATGGAAGGCAAGATCGCGAAGTGGTGGATGCCCGA
CGACGTCGCCTTCGTCGACGGCATCCCGCACACCGCGACCGGCAAGATCCTCAAGACGGCGCTGCGCGAGCAGTTCAAGG
CGTATCGATTCCCGAACGCGGCGGCGTAG

Upstream 100 bases:

>100_bases
CAAGGCCATTTGCGCTCGCTGCGGCGCGCGCTAAAACCGACGAAACCGATCAAAAGACCCTCGCTGTCGAAAAGCGAATA
CCACCCGGAGGAACGACCTG

Downstream 100 bases:

>100_bases
CAGCCGCTCCCGCTCCGCGCGTGACTCACGGCCCCTGCGACCACGCGGGGGCCGTTTGCGTTCCGGTGAAAAAGTTCCTC
TGTCCTGGTTTGAACCAAAG

Product: putative acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLGLMQDWPLLCHRIIEHAATIHGSQEVVTRSVEGPIVRTTYRQIHDRALKVSQKLTRDGIKLGDRVATIAWNTGRHLEV
WYGIMGIGAICHTVNPRLFPEQIGWIINHAQDRIVITDLTFVPLLEKLADKLPSVERYVVLTDAAHMPQTTLKNAVAYES
WIGEADGDFAWGTFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANNRDSLGTSAADTMLPVVPLFHANSWGIAFS
APSMGTKLVMPGPKLDGASVYELLDTEKVTYTAGVPTVWLMLLQHMQAHRLTLPHLKMVVCGGSAMPRSMIQAFLDMGIG
VRHAWGMTEMSPIGTLATLKPPFLEAEGDAKLDILQTQGFPPFGVEMKITDDAGAELPWDGKTFGRLKVSGPAVSKAYYK
VDTKILDDEGFFDTGDVATVDADGFMRITDRSKDVIKSGGEWISSIDLENLAVGHPAVAEAAVIGVYHPKWDERPLLIVQ
LKQGQTATREDILKFMEGKIAKWWMPDDVAFVDGIPHTATGKILKTALREQFKAYRFPNAAA

Sequences:

>Translated_542_residues
MLGLMQDWPLLCHRIIEHAATIHGSQEVVTRSVEGPIVRTTYRQIHDRALKVSQKLTRDGIKLGDRVATIAWNTGRHLEV
WYGIMGIGAICHTVNPRLFPEQIGWIINHAQDRIVITDLTFVPLLEKLADKLPSVERYVVLTDAAHMPQTTLKNAVAYES
WIGEADGDFAWGTFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANNRDSLGTSAADTMLPVVPLFHANSWGIAFS
APSMGTKLVMPGPKLDGASVYELLDTEKVTYTAGVPTVWLMLLQHMQAHRLTLPHLKMVVCGGSAMPRSMIQAFLDMGIG
VRHAWGMTEMSPIGTLATLKPPFLEAEGDAKLDILQTQGFPPFGVEMKITDDAGAELPWDGKTFGRLKVSGPAVSKAYYK
VDTKILDDEGFFDTGDVATVDADGFMRITDRSKDVIKSGGEWISSIDLENLAVGHPAVAEAAVIGVYHPKWDERPLLIVQ
LKQGQTATREDILKFMEGKIAKWWMPDDVAFVDGIPHTATGKILKTALREQFKAYRFPNAAA
>Mature_542_residues
MLGLMQDWPLLCHRIIEHAATIHGSQEVVTRSVEGPIVRTTYRQIHDRALKVSQKLTRDGIKLGDRVATIAWNTGRHLEV
WYGIMGIGAICHTVNPRLFPEQIGWIINHAQDRIVITDLTFVPLLEKLADKLPSVERYVVLTDAAHMPQTTLKNAVAYES
WIGEADGDFAWGTFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANNRDSLGTSAADTMLPVVPLFHANSWGIAFS
APSMGTKLVMPGPKLDGASVYELLDTEKVTYTAGVPTVWLMLLQHMQAHRLTLPHLKMVVCGGSAMPRSMIQAFLDMGIG
VRHAWGMTEMSPIGTLATLKPPFLEAEGDAKLDILQTQGFPPFGVEMKITDDAGAELPWDGKTFGRLKVSGPAVSKAYYK
VDTKILDDEGFFDTGDVATVDADGFMRITDRSKDVIKSGGEWISSIDLENLAVGHPAVAEAAVIGVYHPKWDERPLLIVQ
LKQGQTATREDILKFMEGKIAKWWMPDDVAFVDGIPHTATGKILKTALREQFKAYRFPNAAA

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=527, Percent_Identity=25.6166982922201, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI187761345, Length=538, Percent_Identity=25.8364312267658, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI187761343, Length=538, Percent_Identity=25.8364312267658, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI115511026, Length=514, Percent_Identity=23.3463035019455, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI38505220, Length=485, Percent_Identity=23.0927835051546, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI28416953, Length=541, Percent_Identity=23.6598890942699, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157311624, Length=513, Percent_Identity=23.5867446393762, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=513, Percent_Identity=23.5867446393762, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI58082049, Length=517, Percent_Identity=23.4042553191489, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI42544132, Length=524, Percent_Identity=23.6641221374046, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=518, Percent_Identity=23.3590733590734, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI8923896, Length=385, Percent_Identity=27.012987012987, Blast_Score=80, Evalue=6e-15,
Organism=Homo sapiens, GI253970489, Length=385, Percent_Identity=27.012987012987, Blast_Score=80, Evalue=7e-15,
Organism=Escherichia coli, GI221142682, Length=534, Percent_Identity=24.7191011235955, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=27.037773359841, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788107, Length=519, Percent_Identity=25.8188824662813, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1786810, Length=514, Percent_Identity=25.0972762645914, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789201, Length=506, Percent_Identity=23.9130434782609, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788595, Length=495, Percent_Identity=21.8181818181818, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790505, Length=182, Percent_Identity=29.1208791208791, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=518, Percent_Identity=23.9382239382239, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=506, Percent_Identity=25.296442687747, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=542, Percent_Identity=24.5387453874539, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=537, Percent_Identity=25.3258845437616, Blast_Score=128, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=537, Percent_Identity=25.3258845437616, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=537, Percent_Identity=25.3258845437616, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=542, Percent_Identity=24.3542435424354, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=24.65483234714, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=538, Percent_Identity=22.6765799256506, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=511, Percent_Identity=25.440313111546, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=538, Percent_Identity=23.6059479553903, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=25.8953168044077, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=464, Percent_Identity=22.8448275862069, Blast_Score=93, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=29.8342541436464, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=589, Percent_Identity=24.6179966044143, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI18859661, Length=525, Percent_Identity=27.8095238095238, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24581924, Length=556, Percent_Identity=25.3597122302158, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI21355181, Length=541, Percent_Identity=24.5841035120148, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=23.8888888888889, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24667955, Length=553, Percent_Identity=23.6889692585895, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21356947, Length=513, Percent_Identity=22.8070175438596, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24667959, Length=443, Percent_Identity=24.8306997742664, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648260, Length=498, Percent_Identity=24.6987951807229, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648255, Length=533, Percent_Identity=22.8893058161351, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648253, Length=533, Percent_Identity=22.7016885553471, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653035, Length=517, Percent_Identity=20.6963249516441, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355225, Length=566, Percent_Identity=22.9681978798587, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24648257, Length=518, Percent_Identity=22.2007722007722, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358303, Length=515, Percent_Identity=21.5533980582524, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=524, Percent_Identity=20.4198473282443, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356441, Length=370, Percent_Identity=24.5945945945946, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59570; Mature: 59570

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLMQDWPLLCHRIIEHAATIHGSQEVVTRSVEGPIVRTTYRQIHDRALKVSQKLTRDG
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
IKLGDRVATIAWNTGRHLEVWYGIMGIGAICHTVNPRLFPEQIGWIINHAQDRIVITDLT
HHCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCH
FVPLLEKLADKLPSVERYVVLTDAAHMPQTTLKNAVAYESWIGEADGDFAWGTFDENTAA
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEE
AMCYTSGTTGDPKGVLYSHRSNVLHALMANNRDSLGTSAADTMLPVVPLFHANSWGIAFS
EEEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEECCCCCEEEE
APSMGTKLVMPGPKLDGASVYELLDTEKVTYTAGVPTVWLMLLQHMQAHRLTLPHLKMVV
CCCCCCEEEECCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEE
CGGSAMPRSMIQAFLDMGIGVRHAWGMTEMSPIGTLATLKPPFLEAEGDAKLDILQTQGF
ECCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
PPFGVEMKITDDAGAELPWDGKTFGRLKVSGPAVSKAYYKVDTKILDDEGFFDTGDVATV
CCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHEEEEECCEEECCCCCCCCCCEEEE
DADGFMRITDRSKDVIKSGGEWISSIDLENLAVGHPAVAEAAVIGVYHPKWDERPLLIVQ
CCCCEEEECCCCHHHHHCCCHHHHHCCCCCEECCCCHHHCEEEEEEECCCCCCCCEEEEE
LKQGQTATREDILKFMEGKIAKWWMPDDVAFVDGIPHTATGKILKTALREQFKAYRFPNA
ECCCCCCCHHHHHHHHCCCCEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
AA
CC
>Mature Secondary Structure
MLGLMQDWPLLCHRIIEHAATIHGSQEVVTRSVEGPIVRTTYRQIHDRALKVSQKLTRDG
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCC
IKLGDRVATIAWNTGRHLEVWYGIMGIGAICHTVNPRLFPEQIGWIINHAQDRIVITDLT
HHCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCH
FVPLLEKLADKLPSVERYVVLTDAAHMPQTTLKNAVAYESWIGEADGDFAWGTFDENTAA
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEE
AMCYTSGTTGDPKGVLYSHRSNVLHALMANNRDSLGTSAADTMLPVVPLFHANSWGIAFS
EEEEECCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEECCCCCEEEE
APSMGTKLVMPGPKLDGASVYELLDTEKVTYTAGVPTVWLMLLQHMQAHRLTLPHLKMVV
CCCCCCEEEECCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEE
CGGSAMPRSMIQAFLDMGIGVRHAWGMTEMSPIGTLATLKPPFLEAEGDAKLDILQTQGF
ECCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
PPFGVEMKITDDAGAELPWDGKTFGRLKVSGPAVSKAYYKVDTKILDDEGFFDTGDVATV
CCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCHHHEEEEECCEEECCCCCCCCCCEEEE
DADGFMRITDRSKDVIKSGGEWISSIDLENLAVGHPAVAEAAVIGVYHPKWDERPLLIVQ
CCCCEEEECCCCHHHHHCCCHHHHHCCCCCEECCCCHHHCEEEEEEECCCCCCCCEEEEE
LKQGQTATREDILKFMEGKIAKWWMPDDVAFVDGIPHTATGKILKTALREQFKAYRFPNA
ECCCCCCCHHHHHHHHCCCCEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
AA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]