| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
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The map label for this gene is dkgB [H]
Identifier: 146339169
GI number: 146339169
Start: 2263425
End: 2264246
Strand: Direct
Name: dkgB [H]
Synonym: BRADO2128
Alternate gene names: 146339169
Gene position: 2263425-2264246 (Clockwise)
Preceding gene: 146339168
Following gene: 146339172
Centisome position: 30.35
GC content: 66.42
Gene sequence:
>822_bases ATGAACGTCATCGAAACGCAGGGCGTCCGGCTGCCCAAGCTCGGGCTCGGCACCTTCCGCATGCAGGGCACAGTATGCCG CGACGCGGTCGAGAGCGCGCTCGCGCTCGGCTACCGCCACATCGACACCGCCGAGATGTACGCCAATGAGGATGCGATCG GCGCGGCGCTTGCGGCAGCTCAGATCGCCCGCGAGGATTTGCACGTCACGACAAAGGTCTGGCCGGAGAACCTCGCGCCG GACGCCATCCGCCGCGCGTTCGACACCAGCTTGAAGAAGCTCAGGCTCGACTTCGTCGATCTCTATCTCATTCACTGGCC GGCGACGGGCATGAACCTGCCGGCCGCGATGGAGACGTTGATGGCGCTGAAGCAGGAGGGACGCACGCGCGCGATCGGCG TCGCCAACTTCACCGTCGCGCTGCTCAAGCAGGCCGTCGAGGACATCCAGGCGCCGGTTGCCTGCAATCAGGTCGAGTAT CACGTGATGCTCGATCAGTCGAAGCTGATGGCCTACATGAAGAGCCGCTCGATTCCCCTGGTGGCCTACTGCCCCCTCGC ACAGGGCCGGGCGGCCGGCGACGAGACGCTGGCCGGGATCGGCCGCAAGCACGGCGCTGGCGCCGCCCAGGTCGCGCTGA AATGGCTGCTCGACCAGGATGGCGTCGCCGCCATCCCGAAGGCCGCGCGCCCGGAGAGTCAGCAGGCGAACTGGGATGCG ATGAAGGTTCAACTGGATGATGAGGACCGCGCGACCATCGCCGCGTTGCCGAAGGACAAGCGGTTCGTCAATCCGGGCTT CGCGCCGGATTGGGATCGCTGA
Upstream 100 bases:
>100_bases GGGCCTCAGGCAGTCTGAGCGTATCCCGTCAAGGTCTTGATATACCAAGGCTGCGCCAGTGCGAATCTGGCCACAGGTTT GCGTCGTCCCAGGAGAGATC
Downstream 100 bases:
>100_bases ACGTTCGTCGGCGACAAACTCTGCGCAGAAAAATGGCCCCTCGCGGGGCCATTTCCATCTCTGCGGCGCGGATCAGTCGC GGCGCTTGATGATCACGGTG
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 273; Mature: 273
Protein sequence:
>273_residues MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAAQIAREDLHVTTKVWPENLAP DAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETLMALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEY HVMLDQSKLMAYMKSRSIPLVAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR
Sequences:
>Translated_273_residues MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAAQIAREDLHVTTKVWPENLAP DAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETLMALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEY HVMLDQSKLMAYMKSRSIPLVAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR >Mature_273_residues MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAAQIAREDLHVTTKVWPENLAP DAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETLMALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEY HVMLDQSKLMAYMKSRSIPLVAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=32.2463768115942, Blast_Score=147, Evalue=1e-35, Organism=Homo sapiens, GI310109920, Length=295, Percent_Identity=31.1864406779661, Blast_Score=145, Evalue=5e-35, Organism=Homo sapiens, GI24497585, Length=306, Percent_Identity=31.6993464052288, Blast_Score=144, Evalue=8e-35, Organism=Homo sapiens, GI45446745, Length=295, Percent_Identity=31.1864406779661, Blast_Score=144, Evalue=1e-34, Organism=Homo sapiens, GI4503285, Length=295, Percent_Identity=31.1864406779661, Blast_Score=144, Evalue=1e-34, Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=31.740614334471, Blast_Score=144, Evalue=1e-34, Organism=Homo sapiens, GI5453543, Length=295, Percent_Identity=30.8474576271186, Blast_Score=141, Evalue=5e-34, Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=30.7692307692308, Blast_Score=139, Evalue=3e-33, Organism=Homo sapiens, GI24497583, Length=296, Percent_Identity=30.4054054054054, Blast_Score=135, Evalue=5e-32, Organism=Homo sapiens, GI24497577, Length=305, Percent_Identity=28.8524590163934, Blast_Score=132, Evalue=3e-31, Organism=Homo sapiens, GI5174391, Length=305, Percent_Identity=28.8524590163934, Blast_Score=132, Evalue=3e-31, Organism=Homo sapiens, GI291291012, Length=271, Percent_Identity=31.3653136531365, Blast_Score=128, Evalue=6e-30, Organism=Homo sapiens, GI300116273, Length=278, Percent_Identity=29.8561151079137, Blast_Score=124, Evalue=6e-29, Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=29.2418772563177, Blast_Score=115, Evalue=5e-26, Organism=Homo sapiens, GI93277124, Length=304, Percent_Identity=26.9736842105263, Blast_Score=114, Evalue=1e-25, Organism=Homo sapiens, GI4502049, Length=294, Percent_Identity=30.2721088435374, Blast_Score=112, Evalue=4e-25, Organism=Homo sapiens, GI310109926, Length=109, Percent_Identity=39.4495412844037, Blast_Score=89, Evalue=4e-18, Organism=Homo sapiens, GI207028673, Length=109, Percent_Identity=39.4495412844037, Blast_Score=88, Evalue=8e-18, Organism=Homo sapiens, GI310109924, Length=153, Percent_Identity=30.0653594771242, Blast_Score=70, Evalue=3e-12, Organism=Escherichia coli, GI1786400, Length=266, Percent_Identity=42.1052631578947, Blast_Score=217, Evalue=7e-58, Organism=Escherichia coli, GI87082198, Length=264, Percent_Identity=35.9848484848485, Blast_Score=167, Evalue=5e-43, Organism=Escherichia coli, GI1788081, Length=278, Percent_Identity=34.1726618705036, Blast_Score=115, Evalue=3e-27, Organism=Escherichia coli, GI1788070, Length=311, Percent_Identity=26.3665594855305, Blast_Score=81, Evalue=7e-17, Organism=Escherichia coli, GI1787674, Length=282, Percent_Identity=25.886524822695, Blast_Score=77, Evalue=1e-15, Organism=Escherichia coli, GI87081735, Length=287, Percent_Identity=26.4808362369338, Blast_Score=76, Evalue=2e-15, Organism=Escherichia coli, GI1789375, Length=296, Percent_Identity=25.3378378378378, Blast_Score=72, Evalue=3e-14, Organism=Caenorhabditis elegans, GI71998625, Length=263, Percent_Identity=31.5589353612167, Blast_Score=146, Evalue=1e-35, Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=33.7883959044369, Blast_Score=145, Evalue=2e-35, Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=33.6734693877551, Blast_Score=145, Evalue=3e-35, Organism=Caenorhabditis elegans, GI17566692, Length=293, Percent_Identity=30.0341296928328, Blast_Score=137, Evalue=6e-33, Organism=Caenorhabditis elegans, GI17561298, Length=275, Percent_Identity=29.0909090909091, Blast_Score=136, Evalue=1e-32, Organism=Caenorhabditis elegans, GI17561300, Length=281, Percent_Identity=31.6725978647687, Blast_Score=134, Evalue=5e-32, Organism=Caenorhabditis elegans, GI17538386, Length=275, Percent_Identity=30.9090909090909, Blast_Score=130, Evalue=6e-31, Organism=Caenorhabditis elegans, GI17564128, Length=300, Percent_Identity=31, Blast_Score=128, Evalue=3e-30, Organism=Caenorhabditis elegans, GI17552492, Length=264, Percent_Identity=28.7878787878788, Blast_Score=127, Evalue=4e-30, Organism=Caenorhabditis elegans, GI17550248, Length=294, Percent_Identity=30.2721088435374, Blast_Score=127, Evalue=6e-30, Organism=Caenorhabditis elegans, GI17537079, Length=302, Percent_Identity=26.158940397351, Blast_Score=110, Evalue=7e-25, Organism=Caenorhabditis elegans, GI17562292, Length=288, Percent_Identity=29.5138888888889, Blast_Score=108, Evalue=3e-24, Organism=Saccharomyces cerevisiae, GI6324694, Length=295, Percent_Identity=33.2203389830508, Blast_Score=158, Evalue=1e-39, Organism=Saccharomyces cerevisiae, GI6320576, Length=283, Percent_Identity=32.5088339222615, Blast_Score=150, Evalue=3e-37, Organism=Saccharomyces cerevisiae, GI6321896, Length=312, Percent_Identity=27.5641025641026, Blast_Score=123, Evalue=4e-29, Organism=Saccharomyces cerevisiae, GI6319625, Length=302, Percent_Identity=30.1324503311258, Blast_Score=120, Evalue=3e-28, Organism=Saccharomyces cerevisiae, GI6322556, Length=255, Percent_Identity=26.6666666666667, Blast_Score=103, Evalue=3e-23, Organism=Saccharomyces cerevisiae, GI6320079, Length=233, Percent_Identity=27.4678111587983, Blast_Score=92, Evalue=1e-19, Organism=Drosophila melanogaster, GI21356425, Length=296, Percent_Identity=33.445945945946, Blast_Score=160, Evalue=7e-40, Organism=Drosophila melanogaster, GI24644950, Length=303, Percent_Identity=33.6633663366337, Blast_Score=160, Evalue=1e-39, Organism=Drosophila melanogaster, GI221378297, Length=323, Percent_Identity=31.5789473684211, Blast_Score=155, Evalue=3e-38, Organism=Drosophila melanogaster, GI24657054, Length=292, Percent_Identity=33.2191780821918, Blast_Score=151, Evalue=4e-37, Organism=Drosophila melanogaster, GI45553081, Length=292, Percent_Identity=32.1917808219178, Blast_Score=140, Evalue=1e-33, Organism=Drosophila melanogaster, GI20129731, Length=285, Percent_Identity=30.5263157894737, Blast_Score=129, Evalue=2e-30, Organism=Drosophila melanogaster, GI24663317, Length=293, Percent_Identity=29.0102389078498, Blast_Score=125, Evalue=2e-29, Organism=Drosophila melanogaster, GI24662789, Length=301, Percent_Identity=29.9003322259136, Blast_Score=124, Evalue=1e-28, Organism=Drosophila melanogaster, GI281366140, Length=261, Percent_Identity=31.4176245210728, Blast_Score=116, Evalue=2e-26, Organism=Drosophila melanogaster, GI24662785, Length=279, Percent_Identity=31.1827956989247, Blast_Score=112, Evalue=3e-25, Organism=Drosophila melanogaster, GI24662781, Length=280, Percent_Identity=31.0714285714286, Blast_Score=110, Evalue=1e-24,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29834; Mature: 29834
Theoretical pI: Translated: 6.68; Mature: 6.68
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 3.7 %Met (Translated Protein) 4.8 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 3.7 %Met (Mature Protein) 4.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAA CCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH QIAREDLHVTTKVWPENLAPDAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETL HHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHH MALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEYHVMLDQSKLMAYMKSRSIPL HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHCCCCE VAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA EEECCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCE MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC >Mature Secondary Structure MNVIETQGVRLPKLGLGTFRMQGTVCRDAVESALALGYRHIDTAEMYANEDAIGAALAAA CCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH QIAREDLHVTTKVWPENLAPDAIRRAFDTSLKKLRLDFVDLYLIHWPATGMNLPAAMETL HHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHH MALKQEGRTRAIGVANFTVALLKQAVEDIQAPVACNQVEYHVMLDQSKLMAYMKSRSIPL HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHCCCCE VAYCPLAQGRAAGDETLAGIGRKHGAGAAQVALKWLLDQDGVAAIPKAARPESQQANWDA EEECCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCE MKVQLDDEDRATIAALPKDKRFVNPGFAPDWDR EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]