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The map label for this gene is potH [H]

Identifier: 146339154

GI number: 146339154

Start: 2245275

End: 2246150

Strand: Reverse

Name: potH [H]

Synonym: BRADO2112

Alternate gene names: 146339154

Gene position: 2246150-2245275 (Counterclockwise)

Preceding gene: 146339155

Following gene: 146339153

Centisome position: 30.12

GC content: 61.87

Gene sequence:

>876_bases
ATGCCAGGTAATAGCGCGCCGCGCGTCAACAGACTTGCGCTGTTGATCCTGCCTAGCGCGCTGCTGTTCGCGTTGTTCTT
CTTCCTGCCGATCGGACTGATGGCTGTCATGAGCGTGCTGACGGGAAATCCCGTCGTCACGCCGAACGTCGCGTTCACCA
CCAAGCACTACGCCCGGATGGGCACCGACAGCTATTATCTCGAGGTGATCTGGACTACGCTCCGGATCGGTCTGTGGACC
ACGCTGGCTTCGTTGTTGGTCGGCTACCCGCTGGCGCATTGGATGGCGCGGATCAAGAGCCGCACCGGCCATGCGCTGCT
GCTGATGGCCGTGCTGGCTCCGATGCTGACAGGCATCGTCGTGCGTACCTTCGCCTGGATGACGCTCTTGTCCGACAAGG
GCGTCATCAACCAGATCCTGACGTCGCTCGGACTCATCACCAAGCCGCTGCAGCTGATGTACAACGAGACCGGCATCATC
GTCGGCCTCGTCCACATCTACGTGCCTTTCATGGTCCTGACTTTGACCGGTGTGATCGGACGCATCGACGAGCGACTGGA
GCAGGCCGCAGAAAATCTCGGCGCCAGCCCGTGGCGCGCGTTCGTCGAGGTGACGCTGCCGCTGAGCCTGCCCGGCATCC
TCGCCGGCTCGCTCCTGGTGTTCGCACTGGCGATCAGCGCCTATGTCACGCCGATCCTGCTCGGCGGCTTCCAGATCATG
ACCTTGCCGATTCTGATCTATCAGCAGATCTCGGCGAATTTCAACGTCGGCTTCGCCGCGGCCCTCGGTATGGTACTGCT
GGTCATTTCGCTCGTGCTGGTCATCGCCTACAATCACGTGCTTGGCCTCGTGTCGGGGCAGCGCGAGCTGCGATGA

Upstream 100 bases:

>100_bases
ATGTCCGCCGCGAGCTCGGCGCGATGGCGGCGGAGCTGCGGCGCATGACCGGCGATCCGCTGGGACAGACCATCGAGCAA
TTGGCTGGACTGGCCTCCTG

Downstream 100 bases:

>100_bases
GGGCGCAGATGACGAGCCTTTCCAAGGGATCGCAGCGTCCGTTGGCCGCATCGATCGAACACACGGGCTCGCCGGCGTTG
CGTGGCAGCAAGTCGCCGAT

Product: putative ABC spermidine/putrescine transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MPGNSAPRVNRLALLILPSALLFALFFFLPIGLMAVMSVLTGNPVVTPNVAFTTKHYARMGTDSYYLEVIWTTLRIGLWT
TLASLLVGYPLAHWMARIKSRTGHALLLMAVLAPMLTGIVVRTFAWMTLLSDKGVINQILTSLGLITKPLQLMYNETGII
VGLVHIYVPFMVLTLTGVIGRIDERLEQAAENLGASPWRAFVEVTLPLSLPGILAGSLLVFALAISAYVTPILLGGFQIM
TLPILIYQQISANFNVGFAAALGMVLLVISLVLVIAYNHVLGLVSGQRELR

Sequences:

>Translated_291_residues
MPGNSAPRVNRLALLILPSALLFALFFFLPIGLMAVMSVLTGNPVVTPNVAFTTKHYARMGTDSYYLEVIWTTLRIGLWT
TLASLLVGYPLAHWMARIKSRTGHALLLMAVLAPMLTGIVVRTFAWMTLLSDKGVINQILTSLGLITKPLQLMYNETGII
VGLVHIYVPFMVLTLTGVIGRIDERLEQAAENLGASPWRAFVEVTLPLSLPGILAGSLLVFALAISAYVTPILLGGFQIM
TLPILIYQQISANFNVGFAAALGMVLLVISLVLVIAYNHVLGLVSGQRELR
>Mature_290_residues
PGNSAPRVNRLALLILPSALLFALFFFLPIGLMAVMSVLTGNPVVTPNVAFTTKHYARMGTDSYYLEVIWTTLRIGLWTT
LASLLVGYPLAHWMARIKSRTGHALLLMAVLAPMLTGIVVRTFAWMTLLSDKGVINQILTSLGLITKPLQLMYNETGIIV
GLVHIYVPFMVLTLTGVIGRIDERLEQAAENLGASPWRAFVEVTLPLSLPGILAGSLLVFALAISAYVTPILLGGFQIMT
LPILIYQQISANFNVGFAAALGMVLLVISLVLVIAYNHVLGLVSGQRELR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=224, Percent_Identity=34.8214285714286, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787369, Length=267, Percent_Identity=32.9588014981273, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787713, Length=230, Percent_Identity=32.6086956521739, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31612; Mature: 31480

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
PGNSAPRVNRLALLILPSALLFALFFFLPIGLMAVMSVLTGNPVVTPNVAFTTKHYARM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
GTDSYYLEVIWTTLRIGLWTTLASLLVGYPLAHWMARIKSRTGHALLLMAVLAPMLTGIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VRTFAWMTLLSDKGVINQILTSLGLITKPLQLMYNETGIIVGLVHIYVPFMVLTLTGVIG
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RIDERLEQAAENLGASPWRAFVEVTLPLSLPGILAGSLLVFALAISAYVTPILLGGFQIM
HHHHHHHHHHHHCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TLPILIYQQISANFNVGFAAALGMVLLVISLVLVIAYNHVLGLVSGQRELR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]