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The map label for this gene is aphA [H]

Identifier: 146339135

GI number: 146339135

Start: 2225597

End: 2227609

Strand: Direct

Name: aphA [H]

Synonym: BRADO2089

Alternate gene names: 146339135

Gene position: 2225597-2227609 (Clockwise)

Preceding gene: 146339133

Following gene: 146339136

Centisome position: 29.85

GC content: 68.6

Gene sequence:

>2013_bases
ATGATCAGTCGTGAGTTCGGGCGGACCTTCTGGCGCAATCAGACCTCTGTCGATTACGCGCTTGCGGACCTCGCCGCGGG
CCGCCTCGAGGATCATGATTTCCGGCTGCTCGCGGACGGCATTCCCACGCTGTGCTGGATCGCCAATGGCGACGGCTACA
TCGTCTGGTACAATCGCCGCTGGCACGAATACTGCGGCAGCACGCCGACGCAGATGGAAGGCTGGGGCTGGCAGTCGGTG
CACGACCCCGCGCAGCTTCCCTCCGTGATGCAGCGCTGGCAGGCCTCGATCGCCACCGGCGAGCCGTTCGAGATGACGTT
TCCGCTGCGCGGCGCCGACGGAGTCTTCCGCCCATTCCTCACCCGGATCCAGCCGATGCGCGACGCCTCGGGCCGGATCC
GCCGTTGGTTCGGCGTGAACACCGAAGTGTCGGCGCAGGTCCAGGCCGAGCAGGCGCTGCGCGACAGCGAGACGCAGGCG
CGTGCCGATGCGGAGCGCATCCAGCTCGCGCTGGCGGCGGGCGCCATCATCGGCACCTGGGTCTGGGACATCCCCGCCAA
CCGCTTCACCATCGACGAGAATTTTGCGGTCAATTTCGGTCTCGATCCCGCGCTCGGGCGCGAAGGCCTGAGCCTCGAGC
AGGTCACGATGACCGTGCACCCGGACGATCAGGCCGGGCTCGCCGCCGCCATTCAGGACGCCCTGACGCGCGGCGGCGAC
TACGCGCACCAATATCGCGTCCGCCGTCGCGATGGCCGCTATCACTGGATCGAGGCCAACGGCCGCGTCGAATTGCGCGA
CGGCAAGCCGGTGCGCTTCCCCGGCGTTCTGCTCGACGTCAACGCGCGTCACGCCGCCGAGGCGGCGCTGCGCGAGACCG
AGGAGCGGCTGCGGCTGGCGACCCAGGTCGCCGAGATCGGCTTCTGGGACTACGATCCCGCGACCGGCGTGCTGATCTGG
CCGCCACTCGTCCGGGCGATGTTCGGCGTCGCCGAGGACGTGCCGGTCACGATCGCGGACTTCTACGAAGGCCTGCATCC
GGACGACCGCGAGCCGACGACGGCCGCCTTCGCCGCCGCCGCCGATCCCGCCCAGCGGGCGCTCTACGACGTCGAGTATC
GCACCATCGGCAAGAACGACGGCGCGCTGCGCTGGGTCGCCGCCAAGGGTCGCGGCCTGTTCGACGAGGCCGGTCATTGC
GTGCGCCTGATCGGCACGGCGATCGATATCACCGCGCGCAAGACCGCTGAGGCGCGGCTGCGCGATCTCAACGAGCGGCT
GGAGGTCGCCAATGAGGAGATCAAGCGCTACGCTCATGTGGTGAGCCACGATCTGCGCGCGCCGCTCGTCAACATCATGG
GCTTCGCCCAGGAGCTGCAGAGCCAGCGCGACGAGATGTTCGCCGCCGGGCAGCTGCCCAAGGCCGATCCGCAGCGTGTG
CAGGCCGAGCGCGATTTCGACGAGTCGCTGTCCTTCATCCGCGCCGCGACGCAGAAGATGGACGGCCTGATCACCGCCAT
CCTCAAAGTGTCGCGCCATGGCCAGCAGCCGCTGCATCCCGAGCCGCTCGACATGGAGGCTCTGGTGAGGAAGCTCGCCG
ATGCCATTCGTCATCAGACCGAGACGGCGGGCGCCGTCATCACCATCGAGCCGTTGCCGTCGCTGGTGGCGGATCCGCTC
GCGGTCGGTCAGATCTTCGGCAACCTGCTCGACAATGCGGTCAAATATCTCGATCCCGACCGCCCCGGCCGCATCACGGT
CTCTGGCGAGGCGCGCGGCCGTGAGGTGGTCGTCCATGTCGCCGACAACGGCCGGGGCATCCGCCCGGAGGACCAGGCGC
GGGTGTTCGAGCTGTTTCAGCGCGCCGGCCAGCGCGACCAGCCCGGCGAGGGCATCGGCCTTGCGCATGTGAAGTCCTTG
GTCGGGCGCATGCACGGCAACATCTCGCTGACATCGGAGGCCGGCGTCGGCACGACGTTCACCGTGACCTTGCCGCGCAC
GCCTGCGAACTAA

Upstream 100 bases:

>100_bases
AACTTTGCCCTCACGCGGCCGTTACGTCGATGCGTCCGCTCCCGCCTGTCCGCGATGGTGCGCGATGAGGCGGCAATGTC
GCTGCCGGCCAGGGGGATGG

Downstream 100 bases:

>100_bases
GCGCGCCCGCAAATCAGTCCTGCGTGGAACGCAGGACGATTTTGATTTGCCGCAACGGCGGCGGCTCTCCTTTCGTCAAC
ATGGCGTCGACGAAAAGGAG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MISREFGRTFWRNQTSVDYALADLAAGRLEDHDFRLLADGIPTLCWIANGDGYIVWYNRRWHEYCGSTPTQMEGWGWQSV
HDPAQLPSVMQRWQASIATGEPFEMTFPLRGADGVFRPFLTRIQPMRDASGRIRRWFGVNTEVSAQVQAEQALRDSETQA
RADAERIQLALAAGAIIGTWVWDIPANRFTIDENFAVNFGLDPALGREGLSLEQVTMTVHPDDQAGLAAAIQDALTRGGD
YAHQYRVRRRDGRYHWIEANGRVELRDGKPVRFPGVLLDVNARHAAEAALRETEERLRLATQVAEIGFWDYDPATGVLIW
PPLVRAMFGVAEDVPVTIADFYEGLHPDDREPTTAAFAAAADPAQRALYDVEYRTIGKNDGALRWVAAKGRGLFDEAGHC
VRLIGTAIDITARKTAEARLRDLNERLEVANEEIKRYAHVVSHDLRAPLVNIMGFAQELQSQRDEMFAAGQLPKADPQRV
QAERDFDESLSFIRAATQKMDGLITAILKVSRHGQQPLHPEPLDMEALVRKLADAIRHQTETAGAVITIEPLPSLVADPL
AVGQIFGNLLDNAVKYLDPDRPGRITVSGEARGREVVVHVADNGRGIRPEDQARVFELFQRAGQRDQPGEGIGLAHVKSL
VGRMHGNISLTSEAGVGTTFTVTLPRTPAN

Sequences:

>Translated_670_residues
MISREFGRTFWRNQTSVDYALADLAAGRLEDHDFRLLADGIPTLCWIANGDGYIVWYNRRWHEYCGSTPTQMEGWGWQSV
HDPAQLPSVMQRWQASIATGEPFEMTFPLRGADGVFRPFLTRIQPMRDASGRIRRWFGVNTEVSAQVQAEQALRDSETQA
RADAERIQLALAAGAIIGTWVWDIPANRFTIDENFAVNFGLDPALGREGLSLEQVTMTVHPDDQAGLAAAIQDALTRGGD
YAHQYRVRRRDGRYHWIEANGRVELRDGKPVRFPGVLLDVNARHAAEAALRETEERLRLATQVAEIGFWDYDPATGVLIW
PPLVRAMFGVAEDVPVTIADFYEGLHPDDREPTTAAFAAAADPAQRALYDVEYRTIGKNDGALRWVAAKGRGLFDEAGHC
VRLIGTAIDITARKTAEARLRDLNERLEVANEEIKRYAHVVSHDLRAPLVNIMGFAQELQSQRDEMFAAGQLPKADPQRV
QAERDFDESLSFIRAATQKMDGLITAILKVSRHGQQPLHPEPLDMEALVRKLADAIRHQTETAGAVITIEPLPSLVADPL
AVGQIFGNLLDNAVKYLDPDRPGRITVSGEARGREVVVHVADNGRGIRPEDQARVFELFQRAGQRDQPGEGIGLAHVKSL
VGRMHGNISLTSEAGVGTTFTVTLPRTPAN
>Mature_670_residues
MISREFGRTFWRNQTSVDYALADLAAGRLEDHDFRLLADGIPTLCWIANGDGYIVWYNRRWHEYCGSTPTQMEGWGWQSV
HDPAQLPSVMQRWQASIATGEPFEMTFPLRGADGVFRPFLTRIQPMRDASGRIRRWFGVNTEVSAQVQAEQALRDSETQA
RADAERIQLALAAGAIIGTWVWDIPANRFTIDENFAVNFGLDPALGREGLSLEQVTMTVHPDDQAGLAAAIQDALTRGGD
YAHQYRVRRRDGRYHWIEANGRVELRDGKPVRFPGVLLDVNARHAAEAALRETEERLRLATQVAEIGFWDYDPATGVLIW
PPLVRAMFGVAEDVPVTIADFYEGLHPDDREPTTAAFAAAADPAQRALYDVEYRTIGKNDGALRWVAAKGRGLFDEAGHC
VRLIGTAIDITARKTAEARLRDLNERLEVANEEIKRYAHVVSHDLRAPLVNIMGFAQELQSQRDEMFAAGQLPKADPQRV
QAERDFDESLSFIRAATQKMDGLITAILKVSRHGQQPLHPEPLDMEALVRKLADAIRHQTETAGAVITIEPLPSLVADPL
AVGQIFGNLLDNAVKYLDPDRPGRITVSGEARGREVVVHVADNGRGIRPEDQARVFELFQRAGQRDQPGEGIGLAHVKSL
VGRMHGNISLTSEAGVGTTFTVTLPRTPAN

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788713, Length=261, Percent_Identity=32.9501915708812, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693157, Length=274, Percent_Identity=29.9270072992701, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI48994928, Length=283, Percent_Identity=28.6219081272085, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=27.4900398406374, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790861, Length=251, Percent_Identity=29.8804780876494, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789149, Length=273, Percent_Identity=25.6410256410256, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1786912, Length=270, Percent_Identity=26.2962962962963, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786600, Length=233, Percent_Identity=27.8969957081545, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788381, Length=335, Percent_Identity=22.6865671641791, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI87082128, Length=252, Percent_Identity=23.4126984126984, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786783, Length=247, Percent_Identity=27.1255060728745, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788549, Length=269, Percent_Identity=26.7657992565056, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74369; Mature: 74369

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50113 PAC ; PS50109 HIS_KIN ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISREFGRTFWRNQTSVDYALADLAAGRLEDHDFRLLADGIPTLCWIANGDGYIVWYNRR
CCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCEEEEEECCC
WHEYCGSTPTQMEGWGWQSVHDPAQLPSVMQRWQASIATGEPFEMTFPLRGADGVFRPFL
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
TRIQPMRDASGRIRRWFGVNTEVSAQVQAEQALRDSETQARADAERIQLALAAGAIIGTW
HHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHE
VWDIPANRFTIDENFAVNFGLDPALGREGLSLEQVTMTVHPDDQAGLAAAIQDALTRGGD
EEECCCCEEEECCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCC
YAHQYRVRRRDGRYHWIEANGRVELRDGKPVRFPGVLLDVNARHAAEAALRETEERLRLA
HHHHHHHHHCCCCEEEEECCCEEEECCCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHH
TQVAEIGFWDYDPATGVLIWPPLVRAMFGVAEDVPVTIADFYEGLHPDDREPTTAAFAAA
HHHHHHCCCCCCCCCCEEECHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCCCCHHHHHHH
ADPAQRALYDVEYRTIGKNDGALRWVAAKGRGLFDEAGHCVRLIGTAIDITARKTAEARL
CCHHHHHHEEEHEEEECCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHEEEHHHHHHHHH
RDLNERLEVANEEIKRYAHVVSHDLRAPLVNIMGFAQELQSQRDEMFAAGQLPKADPQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QAERDFDESLSFIRAATQKMDGLITAILKVSRHGQQPLHPEPLDMEALVRKLADAIRHQT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ETAGAVITIEPLPSLVADPLAVGQIFGNLLDNAVKYLDPDRPGRITVSGEARGREVVVHV
HCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEE
ADNGRGIRPEDQARVFELFQRAGQRDQPGEGIGLAHVKSLVGRMHGNISLTSEAGVGTTF
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEE
TVTLPRTPAN
EEEECCCCCC
>Mature Secondary Structure
MISREFGRTFWRNQTSVDYALADLAAGRLEDHDFRLLADGIPTLCWIANGDGYIVWYNRR
CCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCEEEEEECCCEEEEEECCC
WHEYCGSTPTQMEGWGWQSVHDPAQLPSVMQRWQASIATGEPFEMTFPLRGADGVFRPFL
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHH
TRIQPMRDASGRIRRWFGVNTEVSAQVQAEQALRDSETQARADAERIQLALAAGAIIGTW
HHHHHHHCCCCCEEHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHE
VWDIPANRFTIDENFAVNFGLDPALGREGLSLEQVTMTVHPDDQAGLAAAIQDALTRGGD
EEECCCCEEEECCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCC
YAHQYRVRRRDGRYHWIEANGRVELRDGKPVRFPGVLLDVNARHAAEAALRETEERLRLA
HHHHHHHHHCCCCEEEEECCCEEEECCCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHH
TQVAEIGFWDYDPATGVLIWPPLVRAMFGVAEDVPVTIADFYEGLHPDDREPTTAAFAAA
HHHHHHCCCCCCCCCCEEECHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCCCCHHHHHHH
ADPAQRALYDVEYRTIGKNDGALRWVAAKGRGLFDEAGHCVRLIGTAIDITARKTAEARL
CCHHHHHHEEEHEEEECCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHEEEHHHHHHHHH
RDLNERLEVANEEIKRYAHVVSHDLRAPLVNIMGFAQELQSQRDEMFAAGQLPKADPQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
QAERDFDESLSFIRAATQKMDGLITAILKVSRHGQQPLHPEPLDMEALVRKLADAIRHQT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ETAGAVITIEPLPSLVADPLAVGQIFGNLLDNAVKYLDPDRPGRITVSGEARGREVVVHV
HCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEE
ADNGRGIRPEDQARVFELFQRAGQRDQPGEGIGLAHVKSLVGRMHGNISLTSEAGVGTTF
ECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEE
TVTLPRTPAN
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]