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The map label for this gene is nadB [H]

Identifier: 146339082

GI number: 146339082

Start: 2168200

End: 2169843

Strand: Reverse

Name: nadB [H]

Synonym: BRADO2036

Alternate gene names: 146339082

Gene position: 2169843-2168200 (Counterclockwise)

Preceding gene: 146339089

Following gene: 146339078

Centisome position: 29.1

GC content: 70.01

Gene sequence:

>1644_bases
GTGGAATTTGGACAGCCGATGCACTGCGACCAGATCGAAACCTCAAGCCGCGACACGTCAGGTCAGGACACGTCAAGTCA
CGTCGTGATCGTCGGCGCCGGCGTCGCGGGATTGTATTGCGCGCTGAAGCTCGCACCGCGCCCTGTCATCTTGATCTCCG
CAACGTCGCTCGCAGAGGACTCGTTCGGCGCGACGATGCAGGCCGATATCGCCGCGGCGGTATCTGCGGGCGACAGCCCC
GAAAGCCATGCCGCCGACATGATCGCCGCCGGCTGCGGCCTCGTGGACGAAGATGCGGCGCGCGCGCTCGCGCGTGATGC
GGCAGCACGGATCGAGGATCTCGCGCGCCTCGGCGTGACGTTCGATCGCGACCCCAGGGGCGCACTGGCGACGTCGCGGG
CAGCCGTTCATTCCGCGCGGCGCATCCTGAGCGTGCGCGGCGACTTCACCGGACAGGTCATCATGGCGGCCCTCGCCAAG
GCCGTGCGCGCCAGCCCCTCGATCAGGCTGATGGAGGGATACGTCGCTGACGCGCTGCTGACCGACGATGGCGCCGTGCG
CGGTCTGCGGCTGCGTCGTGCGGATGATGCTGCCGCGGCGCCCTTGCTGATCGCCGCCCCGACGATCGTGCTCGCCACCG
GCGGCGTCGGCCATCTGTTCGCCGTGACCACCAACCCTGCTCATGCGAACGGCATCGGCCTTGCGATCGCCGCGCGCGCC
GGCGCTGTCGTTGCGGATCCGGAGTTCGTGCAATTCCATCCGACCGCGATGGCGGGACAGGATCCGGCGCCAGTGGTCAG
CGAGAGCCTGCGCGGCGCCGGCGCGATCCTGATCAACCGGCGCGGTCAGCGATTCATGCTGCCGCGTCATCCGCTGGTCG
ATCTCGCGCCGCGCGACACGGTTGCGCGCGGCATCATGGCCGAGATCACGGCCGGGCGCGGCGCCTTTCTGGATGCGCGC
GACGTGCTCGATGAGGATGTCGCGGAGCGCTATCCGGTGCTGCACCGTGCCTGCGTCGCCGCCGGGCTCGATCCGACCCG
GCAACCGATTCCGGTGGCACCGGCGGCGCATTTCCACATGGGAGGCATCGCCGTGGATGCGCGCGGCCGCAGCTCGATCG
ACGGGCTGTGGGCGGCCGGCGAGGTCGCCGCGACGGCGACCGATGGCGCCAATCACATCGCTGGCAACGCGCTGCTTGGG
GCCCTGGTGTTCGCCAACAGGATCGCTGAGGACATTGCGGGCCGAACCTTCGCGCCGTGTCCTGCCCTGCATGACGTCAA
GTTCGCGGCGCCAGCCGGGATCGATGCCCCATGGGGCGAGCAGATGCTGCGCGATATCATGACCGGTCAGGTCGGGGTGA
TCCGCGACGAGGACGGCCTGGCCGAAGCTGTCACGACGATCGCCCGGCTCGAGCGCGATAGCCTCGACCCCGCGCTCCGC
AACATGGCGAGCGCAGCGCTCCTGATCGCGACCGCCGCCTGGAGCCGGCGCGAGAGCCGCGGCGCGCATTGCCGGGTCGA
TTGTCCGGCGGAAAATCCGGCCTTCGCGCAGCGGACCATGACCACGCTCGCCGCTGCGCGCGAAATCGCCGACGAGCTCG
CGGCACGGCCGACGACGCGGCTGCCACGATCCTTGAGCGCGTAG

Upstream 100 bases:

>100_bases
CGTGCAGAACAGCCGCGATTTGTCGCCAAACACCTCTAAACAACGAGGGATGTCTCTGAAGAAGAGCTAACTGGTAGCTT
GATACATATGTGCACCGAAT

Downstream 100 bases:

>100_bases
CGCCGCTGATCTTCTCTGTCATAACCCCGTCGTTCCAGCGCAAGGCCGCAGGCCTTGAACCCGGAACCTCGCGCTTGTTT
TGCGCGCATCGCCAGCCAGC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MEFGQPMHCDQIETSSRDTSGQDTSSHVVIVGAGVAGLYCALKLAPRPVILISATSLAEDSFGATMQADIAAAVSAGDSP
ESHAADMIAAGCGLVDEDAARALARDAAARIEDLARLGVTFDRDPRGALATSRAAVHSARRILSVRGDFTGQVIMAALAK
AVRASPSIRLMEGYVADALLTDDGAVRGLRLRRADDAAAAPLLIAAPTIVLATGGVGHLFAVTTNPAHANGIGLAIAARA
GAVVADPEFVQFHPTAMAGQDPAPVVSESLRGAGAILINRRGQRFMLPRHPLVDLAPRDTVARGIMAEITAGRGAFLDAR
DVLDEDVAERYPVLHRACVAAGLDPTRQPIPVAPAAHFHMGGIAVDARGRSSIDGLWAAGEVAATATDGANHIAGNALLG
ALVFANRIAEDIAGRTFAPCPALHDVKFAAPAGIDAPWGEQMLRDIMTGQVGVIRDEDGLAEAVTTIARLERDSLDPALR
NMASAALLIATAAWSRRESRGAHCRVDCPAENPAFAQRTMTTLAAAREIADELAARPTTRLPRSLSA

Sequences:

>Translated_547_residues
MEFGQPMHCDQIETSSRDTSGQDTSSHVVIVGAGVAGLYCALKLAPRPVILISATSLAEDSFGATMQADIAAAVSAGDSP
ESHAADMIAAGCGLVDEDAARALARDAAARIEDLARLGVTFDRDPRGALATSRAAVHSARRILSVRGDFTGQVIMAALAK
AVRASPSIRLMEGYVADALLTDDGAVRGLRLRRADDAAAAPLLIAAPTIVLATGGVGHLFAVTTNPAHANGIGLAIAARA
GAVVADPEFVQFHPTAMAGQDPAPVVSESLRGAGAILINRRGQRFMLPRHPLVDLAPRDTVARGIMAEITAGRGAFLDAR
DVLDEDVAERYPVLHRACVAAGLDPTRQPIPVAPAAHFHMGGIAVDARGRSSIDGLWAAGEVAATATDGANHIAGNALLG
ALVFANRIAEDIAGRTFAPCPALHDVKFAAPAGIDAPWGEQMLRDIMTGQVGVIRDEDGLAEAVTTIARLERDSLDPALR
NMASAALLIATAAWSRRESRGAHCRVDCPAENPAFAQRTMTTLAAAREIADELAARPTTRLPRSLSA
>Mature_547_residues
MEFGQPMHCDQIETSSRDTSGQDTSSHVVIVGAGVAGLYCALKLAPRPVILISATSLAEDSFGATMQADIAAAVSAGDSP
ESHAADMIAAGCGLVDEDAARALARDAAARIEDLARLGVTFDRDPRGALATSRAAVHSARRILSVRGDFTGQVIMAALAK
AVRASPSIRLMEGYVADALLTDDGAVRGLRLRRADDAAAAPLLIAAPTIVLATGGVGHLFAVTTNPAHANGIGLAIAARA
GAVVADPEFVQFHPTAMAGQDPAPVVSESLRGAGAILINRRGQRFMLPRHPLVDLAPRDTVARGIMAEITAGRGAFLDAR
DVLDEDVAERYPVLHRACVAAGLDPTRQPIPVAPAAHFHMGGIAVDARGRSSIDGLWAAGEVAATATDGANHIAGNALLG
ALVFANRIAEDIAGRTFAPCPALHDVKFAAPAGIDAPWGEQMLRDIMTGQVGVIRDEDGLAEAVTTIARLERDSLDPALR
NMASAALLIATAAWSRRESRGAHCRVDCPAENPAFAQRTMTTLAAAREIADELAARPTTRLPRSLSA

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=488, Percent_Identity=30.327868852459, Blast_Score=166, Evalue=6e-41,
Organism=Escherichia coli, GI1788928, Length=528, Percent_Identity=35.0378787878788, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1790597, Length=496, Percent_Identity=30.241935483871, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786942, Length=497, Percent_Identity=30.3822937625755, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17550100, Length=489, Percent_Identity=30.4703476482618, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17505833, Length=488, Percent_Identity=29.5081967213115, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71986328, Length=422, Percent_Identity=28.9099526066351, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322701, Length=514, Percent_Identity=29.3774319066148, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322416, Length=489, Percent_Identity=30.879345603272, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17137288, Length=487, Percent_Identity=30.8008213552361, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24655642, Length=487, Percent_Identity=30.8008213552361, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24655647, Length=487, Percent_Identity=30.8008213552361, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24663005, Length=506, Percent_Identity=28.8537549407115, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56743; Mature: 56743

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00626 RCC1_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFGQPMHCDQIETSSRDTSGQDTSSHVVIVGAGVAGLYCALKLAPRPVILISATSLAED
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHEECCCCEEEEEECHHCCC
SFGATMQADIAAAVSAGDSPESHAADMIAAGCGLVDEDAARALARDAAARIEDLARLGVT
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
FDRDPRGALATSRAAVHSARRILSVRGDFTGQVIMAALAKAVRASPSIRLMEGYVADALL
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHEE
TDDGAVRGLRLRRADDAAAAPLLIAAPTIVLATGGVGHLFAVTTNPAHANGIGLAIAARA
CCCCCCCCEEEECCCCHHHCCEEEECCEEEEECCCCCEEEEEECCCCCCCCCEEEEEECC
GAVVADPEFVQFHPTAMAGQDPAPVVSESLRGAGAILINRRGQRFMLPRHPLVDLAPRDT
CEEEECCCEEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCHHH
VARGIMAEITAGRGAFLDARDVLDEDVAERYPVLHRACVAAGLDPTRQPIPVAPAAHFHM
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEC
GGIAVDARGRSSIDGLWAAGEVAATATDGANHIAGNALLGALVFANRIAEDIAGRTFAPC
CCEEEECCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PALHDVKFAAPAGIDAPWGEQMLRDIMTGQVGVIRDEDGLAEAVTTIARLERDSLDPALR
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHH
NMASAALLIATAAWSRRESRGAHCRVDCPAENPAFAQRTMTTLAAAREIADELAARPTTR
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
LPRSLSA
CCCCCCC
>Mature Secondary Structure
MEFGQPMHCDQIETSSRDTSGQDTSSHVVIVGAGVAGLYCALKLAPRPVILISATSLAED
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHEECCCCEEEEEECHHCCC
SFGATMQADIAAAVSAGDSPESHAADMIAAGCGLVDEDAARALARDAAARIEDLARLGVT
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
FDRDPRGALATSRAAVHSARRILSVRGDFTGQVIMAALAKAVRASPSIRLMEGYVADALL
ECCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHEE
TDDGAVRGLRLRRADDAAAAPLLIAAPTIVLATGGVGHLFAVTTNPAHANGIGLAIAARA
CCCCCCCCEEEECCCCHHHCCEEEECCEEEEECCCCCEEEEEECCCCCCCCCEEEEEECC
GAVVADPEFVQFHPTAMAGQDPAPVVSESLRGAGAILINRRGQRFMLPRHPLVDLAPRDT
CEEEECCCEEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCEEECCCCCCCCCCCHHH
VARGIMAEITAGRGAFLDARDVLDEDVAERYPVLHRACVAAGLDPTRQPIPVAPAAHFHM
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEC
GGIAVDARGRSSIDGLWAAGEVAATATDGANHIAGNALLGALVFANRIAEDIAGRTFAPC
CCEEEECCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PALHDVKFAAPAGIDAPWGEQMLRDIMTGQVGVIRDEDGLAEAVTTIARLERDSLDPALR
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHH
NMASAALLIATAAWSRRESRGAHCRVDCPAENPAFAQRTMTTLAAAREIADELAARPTTR
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
LPRSLSA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]