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The map label for this gene is ispB [H]

Identifier: 146339069

GI number: 146339069

Start: 2154643

End: 2155653

Strand: Reverse

Name: ispB [H]

Synonym: BRADO2023

Alternate gene names: 146339069

Gene position: 2155653-2154643 (Counterclockwise)

Preceding gene: 146339072

Following gene: 146339065

Centisome position: 28.91

GC content: 66.07

Gene sequence:

>1011_bases
GTGGCGGTTATCGTACCTTTCGAGAGTCCATCGGGCGCCTCCATCGATGGGCTGGTCGGGCTGGTCACAGCCGACATGGA
ACGAGTCAACGCCACGATCCTGTCGCGCACCGGCTCCGACGTGACGATGATTCCAGAGGTCGCCAACCACCTGATCTCCT
CGGGAGGCAAGCGGCTGCGCCCGATGCTGACGCTCGCGATGGCGCATCTCACCGAGTATGCCGGCGACGGTCACGTCAAG
CTCGCTGCTGCCGTCGAGTTCATGCACACCGCGACTTTGCTGCATGACGACGTGGTTGATGAAAGTGAAATGCGCCGCGG
CAAATTGTCCGCGCGCATGCTGTGGGGCAACGAGGCCAGTGTGCTGGTCGGCGACTTCCTGCTCGGACAGGCGTTCCGCA
TGATGGTCGAGGTCGGCTCGCTGCGCGCGCTCGACATCCTCTCGGCGGCCGCCGCCACCATCGCCGAGGGCGAGGTGATG
CAGCTCGCCGCCGCCAAGAACACCGCGACGACGGAGGACGAATATCTCGCCGTCATCCGCGGCAAGACCGCGGAATTGTT
CGCCGCCGCCTGCGAGGTCGGACCCGTGCTCGCCGACCGCCCCAAGGCCGAGCAGACCGCGTCGCGCTCGTTCGGCATGA
ACCTCGGCATCGCCTTCCAGCTCGTCGACGACGTGCTCGACTATGGCGGCAAGGCCGCCAAGCTCGGCAAGAATGTCGGC
GACGATTTCCGCGAGGGCAAGATCACGCTGCCCGTGGTGCTCGCCTTCCGCCGCGGCAGCGACAGCGAGCGCGCGTTCTG
GATCAAGTCGCTGGAGCGCGGCGAGATCAATGACAGCGATCTCGATCACGCCATCGGCCTGATGAACAAGCACCGCGCGC
TCGAGGACACGATCAGCCGCGCGCATCATTACGGCGCCATGGCCGTGGATGCGCTCGCGCTGTTCCCGGCCTCGCCGATG
AAGACCGCGCTGGAGCAGGTCGTGGCGTTCTGTCTGGCGCGGTCGCATTAG

Upstream 100 bases:

>100_bases
AGGGCTTCCAAAGGCTCGGGTCAAGGTTCCGTGTCCGCCCTTGCCGCCCGCGGCCGCAGTTTCTATTGTCGTTTCGAGCA
TAGCAAAATCGGAGACAAGC

Downstream 100 bases:

>100_bases
TTCGCGGTCGTATGCCTGCCCCACTGACGCTGTCATGCCCCCCGCGCACGCGGTGCCGGTGAATGCGCCCTCGCCCCTTG
TGGGAGAGGGCATCGCCGGA

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAVIVPFESPSGASIDGLVGLVTADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLRPMLTLAMAHLTEYAGDGHVK
LAAAVEFMHTATLLHDDVVDESEMRRGKLSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSAAAATIAEGEVM
QLAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPVLADRPKAEQTASRSFGMNLGIAFQLVDDVLDYGGKAAKLGKNVG
DDFREGKITLPVVLAFRRGSDSERAFWIKSLERGEINDSDLDHAIGLMNKHRALEDTISRAHHYGAMAVDALALFPASPM
KTALEQVVAFCLARSH

Sequences:

>Translated_336_residues
MAVIVPFESPSGASIDGLVGLVTADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLRPMLTLAMAHLTEYAGDGHVK
LAAAVEFMHTATLLHDDVVDESEMRRGKLSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSAAAATIAEGEVM
QLAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPVLADRPKAEQTASRSFGMNLGIAFQLVDDVLDYGGKAAKLGKNVG
DDFREGKITLPVVLAFRRGSDSERAFWIKSLERGEINDSDLDHAIGLMNKHRALEDTISRAHHYGAMAVDALALFPASPM
KTALEQVVAFCLARSH
>Mature_335_residues
AVIVPFESPSGASIDGLVGLVTADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLRPMLTLAMAHLTEYAGDGHVKL
AAAVEFMHTATLLHDDVVDESEMRRGKLSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSAAAATIAEGEVMQ
LAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPVLADRPKAEQTASRSFGMNLGIAFQLVDDVLDYGGKAAKLGKNVGD
DFREGKITLPVVLAFRRGSDSERAFWIKSLERGEINDSDLDHAIGLMNKHRALEDTISRAHHYGAMAVDALALFPASPMK
TALEQVVAFCLARSH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=298, Percent_Identity=28.8590604026846, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI4758430, Length=211, Percent_Identity=24.6445497630332, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI83700220, Length=211, Percent_Identity=24.6445497630332, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI169808399, Length=263, Percent_Identity=23.574144486692, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1789578, Length=304, Percent_Identity=45.0657894736842, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI1786623, Length=223, Percent_Identity=32.2869955156951, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=266, Percent_Identity=32.3308270676692, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6319475, Length=307, Percent_Identity=30.6188925081433, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325188, Length=233, Percent_Identity=24.0343347639485, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651612, Length=309, Percent_Identity=29.4498381877023, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281365769, Length=214, Percent_Identity=24.2990654205607, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24660002, Length=214, Percent_Identity=24.2990654205607, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35964; Mature: 35833

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIVPFESPSGASIDGLVGLVTADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLR
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHH
PMLTLAMAHLTEYAGDGHVKLAAAVEFMHTATLLHDDVVDESEMRRGKLSARMLWGNEAS
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCH
VLVGDFLLGQAFRMMVEVGSLRALDILSAAAATIAEGEVMQLAAAKNTATTEDEYLAVIR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHEEEEEE
GKTAELFAAACEVGPVLADRPKAEQTASRSFGMNLGIAFQLVDDVLDYGGKAAKLGKNVG
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCCC
DDFREGKITLPVVLAFRRGSDSERAFWIKSLERGEINDSDLDHAIGLMNKHRALEDTISR
CHHHCCCEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AHHYGAMAVDALALFPASPMKTALEQVVAFCLARSH
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVIVPFESPSGASIDGLVGLVTADMERVNATILSRTGSDVTMIPEVANHLISSGGKRLR
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCHHHH
PMLTLAMAHLTEYAGDGHVKLAAAVEFMHTATLLHDDVVDESEMRRGKLSARMLWGNEAS
HHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCH
VLVGDFLLGQAFRMMVEVGSLRALDILSAAAATIAEGEVMQLAAAKNTATTEDEYLAVIR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHEEEEEE
GKTAELFAAACEVGPVLADRPKAEQTASRSFGMNLGIAFQLVDDVLDYGGKAAKLGKNVG
CCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHCCCCC
DDFREGKITLPVVLAFRRGSDSERAFWIKSLERGEINDSDLDHAIGLMNKHRALEDTISR
CHHHCCCEEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AHHYGAMAVDALALFPASPMKTALEQVVAFCLARSH
HHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]