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The map label for this gene is trmL [H]

Identifier: 146339026

GI number: 146339026

Start: 2100991

End: 2101476

Strand: Reverse

Name: trmL [H]

Synonym: BRADO1975

Alternate gene names: 146339026

Gene position: 2101476-2100991 (Counterclockwise)

Preceding gene: 146339030

Following gene: 146339025

Centisome position: 28.18

GC content: 66.87

Gene sequence:

>486_bases
ATGCAGATCGCGCTCTATCAGCCCGACATTCCGCAGAACACCGGAACGATTCTGCGCCTGTGCGCCTGTCTCGGCCTCGC
CGCCCACATCATCGAGCCGGCGGGCTTTGCCGTCTCCGATCGGCAGTTCCGCCGTGCCGGCATGGACTATCTCGACCATC
TCGACTGGCGGCGGCACGATTCCTGGAGCGCGTTCGAGGCGTGGCGGGCCACGCAAGGCTACCGCCTGCTGCTGCTCACC
ACCAAGGGCGCGACGTCCTACCTGGATATCAGTTACCGCCCGGACGACATCCTGCTGCTCGGGCGCGAGAGCGTCGGCGT
GCCCGCGACGGTGGCAGACGCGGCCGATGCACGGCTGCGCATTCCGCTCAAGCCAGGCATGCGCTCGCTGAACGTGGCGA
TCGCATCGGCCATGGCCGCCGGAGAGGCGCTGCGCCAGGTTGGACATTTCGTCCAATTGCAATCTGCGGAAGGAGCGCGG
GAGTGA

Upstream 100 bases:

>100_bases
GGCCCCGCAGCACGCCATGCACCAATTCCCGCCGGCTCAGCGGACTTTCGGCCTCATCCTTAAGACCAATTCCAAGCCCC
GTTTCCTAAGACCTGTTCGC

Downstream 100 bases:

>100_bases
GCTACGCGGTCAAGGAAATCTTCATGACCCTGCAGGGCGAAGGCGCCCAGGCGGGGCGTGCCGCCGTGTTCTGCCGCTTC
GCCGGCTGCAATCTGTGGAG

Product: putative tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MQIALYQPDIPQNTGTILRLCACLGLAAHIIEPAGFAVSDRQFRRAGMDYLDHLDWRRHDSWSAFEAWRATQGYRLLLLT
TKGATSYLDISYRPDDILLLGRESVGVPATVADAADARLRIPLKPGMRSLNVAIASAMAAGEALRQVGHFVQLQSAEGAR
E

Sequences:

>Translated_161_residues
MQIALYQPDIPQNTGTILRLCACLGLAAHIIEPAGFAVSDRQFRRAGMDYLDHLDWRRHDSWSAFEAWRATQGYRLLLLT
TKGATSYLDISYRPDDILLLGRESVGVPATVADAADARLRIPLKPGMRSLNVAIASAMAAGEALRQVGHFVQLQSAEGAR
E
>Mature_161_residues
MQIALYQPDIPQNTGTILRLCACLGLAAHIIEPAGFAVSDRQFRRAGMDYLDHLDWRRHDSWSAFEAWRATQGYRLLLLT
TKGATSYLDISYRPDDILLLGRESVGVPATVADAADARLRIPLKPGMRSLNVAIASAMAAGEALRQVGHFVQLQSAEGAR
E

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=151, Percent_Identity=44.3708609271523, Blast_Score=130, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17691; Mature: 17691

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIALYQPDIPQNTGTILRLCACLGLAAHIIEPAGFAVSDRQFRRAGMDYLDHLDWRRHD
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCCC
SWSAFEAWRATQGYRLLLLTTKGATSYLDISYRPDDILLLGRESVGVPATVADAADARLR
CCHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCEEECCCCCCEEE
IPLKPGMRSLNVAIASAMAAGEALRQVGHFVQLQSAEGARE
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
>Mature Secondary Structure
MQIALYQPDIPQNTGTILRLCACLGLAAHIIEPAGFAVSDRQFRRAGMDYLDHLDWRRHD
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHHCCCCCCC
SWSAFEAWRATQGYRLLLLTTKGATSYLDISYRPDDILLLGRESVGVPATVADAADARLR
CCHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCCCCCEEECCCCCCEEE
IPLKPGMRSLNVAIASAMAAGEALRQVGHFVQLQSAEGARE
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA