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The map label for this gene is lcfB [H]

Identifier: 146338781

GI number: 146338781

Start: 1831397

End: 1832947

Strand: Direct

Name: lcfB [H]

Synonym: BRADO1715

Alternate gene names: 146338781

Gene position: 1831397-1832947 (Clockwise)

Preceding gene: 146338780

Following gene: 146338782

Centisome position: 24.56

GC content: 65.18

Gene sequence:

>1551_bases
ATGAGGGATCTCTTGAATTTCGGCCAGATGCTATCGGCTCACGCGCGCATGTCGCCGGAGCGCACCGGCGCCCGCGACCT
CGAGCGCGCGATGAGCTTTTCGCTCTGGAACGATCGCGCCTGCCGGCTCGCCAACGCGCTGCTCGGACTTGGCCTCGCCA
AGGGCGACCGCGTCGCGATCCTGGCCTACAACTGCGTCGAGTGGTGCGAGATCTATGCGGCCACGGCCAAGGCCGGCCTC
GTCGCGCTGCCGATCAATTTCCGCCTCACTGCGCCGGAGGTGCAGTTCATCGTCCAGAACGCCGAAGCGTCTGCCATCAT
CGTGCAGGACGATCTCATCGGCCTCGTCGAGGAGGCGCGCAAGGATCTCGGCATTGCGGCCGATCGCTTCATCCATTTCG
GCACCAAGCCGCGTCCAGCGGGTTATCGCGGCTATGAGGACATCATCGCACAGGCGGCGTCGCGCGAGCCGGACCAGCAG
GTTGCGCTGTCCGATCCCTGGACCTTGATGTACACCTCGGGCACGACCGGCAAGCCGAAGGGCGTGCTGCGCAGCCATCG
CAGCGCCGTGCTGCTGTCGATGGTCACCGAGATCGAGCTGAAACTGAGCCGCAATGACGGCGCGCTCCTGGTGATGCCGA
TGTGCCATGCCAACTCGCTGAACTTCTTCGGTGCCTTCGGCTATGTCGGCGGGGTCAACTCGATCTATTCGCGCAAGAGT
TTTGATCCCGAGCACGCGGTGACGACGCTCGCCGACGGCGGCTCGACCTTCACCTCGCTGGTGCCGACGCACTACATCAT
GATGCTCGATCTGCCGCGCGCGATCCGGACGCAGCACGATTTCAGCGCGGTCACCAAGCTGATGATCTCCTCGGCCCCGG
CACGCCCGGAGACCAAGCGCGAGGTGATGGAGATGTTCCCGAAGTCGGGCCTCTACGAGCTCTACGGCGCAACCGAGACA
GGCTGGGTGACCATGCTGCATCCGCATGAGCAGTTCACCAAGCTCGGCTCCGTGGGGCGCGAATGCGTCGGCGCCGCGCC
GATCCGCATCCTCGATGAAGCCGGCAACGAGGTGCCGGACGGCGAGGCGGGCGAGCTCTATTCCTCCAACGCGCACACCT
TCGACTGCTACTGGCGCCTGCCTGAAAAGACCAAGGAGGCGTTCCGCGGCGACTATTGCTCGGTCGGCGACATGGCGCGC
CGCGACGCCGACGGCTACATCCACCTCGCCGACCGCAAGAGCAACATGATCATCTCCGGCGGCGAGAACATTTATCCGTC
AGAGGTCGAGGCGCTGGTCGGCGCGCATCCCGGCGTGAAGGACATCGCGGTGGTCGGCCTGCCCGACGACAAATGGGGCG
AGCGCGTTCATGCCGTGATCGTGCCGCGCGACGGCACGACGGTGAAGGAGTGCGAGCTGGTCGACTGGGCCAAGGAGCGT
CTCGCCGGCTTCAAGCGGCCGCGCAGCTACGCATTCATCACCGACAGCGAGATGCCGCGCAATGCCACCGGCAAGATCCT
GCACCGCGAGCTGAAGAAGCGGTTTTCGTAA

Upstream 100 bases:

>100_bases
GCGCTGCTGATCGGCACGATCGTGATCGCGGCGGTGCCGTGGTTCTCGATCGGCCTTTTGCACTGATCCTTCGGCATATC
AAGAGGTGAGGGGAACGACC

Downstream 100 bases:

>100_bases
GGTGAGTGGTTGCGATGTGGTGCGGTCTGATAGATAGCTGCGATGCAGGCCGTTCTCAACTCCGCTCTTCCCATCTTCGC
GCTGATCCTCACGGGGTTCC

Product: putative long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MRDLLNFGQMLSAHARMSPERTGARDLERAMSFSLWNDRACRLANALLGLGLAKGDRVAILAYNCVEWCEIYAATAKAGL
VALPINFRLTAPEVQFIVQNAEASAIIVQDDLIGLVEEARKDLGIAADRFIHFGTKPRPAGYRGYEDIIAQAASREPDQQ
VALSDPWTLMYTSGTTGKPKGVLRSHRSAVLLSMVTEIELKLSRNDGALLVMPMCHANSLNFFGAFGYVGGVNSIYSRKS
FDPEHAVTTLADGGSTFTSLVPTHYIMMLDLPRAIRTQHDFSAVTKLMISSAPARPETKREVMEMFPKSGLYELYGATET
GWVTMLHPHEQFTKLGSVGRECVGAAPIRILDEAGNEVPDGEAGELYSSNAHTFDCYWRLPEKTKEAFRGDYCSVGDMAR
RDADGYIHLADRKSNMIISGGENIYPSEVEALVGAHPGVKDIAVVGLPDDKWGERVHAVIVPRDGTTVKECELVDWAKER
LAGFKRPRSYAFITDSEMPRNATGKILHRELKKRFS

Sequences:

>Translated_516_residues
MRDLLNFGQMLSAHARMSPERTGARDLERAMSFSLWNDRACRLANALLGLGLAKGDRVAILAYNCVEWCEIYAATAKAGL
VALPINFRLTAPEVQFIVQNAEASAIIVQDDLIGLVEEARKDLGIAADRFIHFGTKPRPAGYRGYEDIIAQAASREPDQQ
VALSDPWTLMYTSGTTGKPKGVLRSHRSAVLLSMVTEIELKLSRNDGALLVMPMCHANSLNFFGAFGYVGGVNSIYSRKS
FDPEHAVTTLADGGSTFTSLVPTHYIMMLDLPRAIRTQHDFSAVTKLMISSAPARPETKREVMEMFPKSGLYELYGATET
GWVTMLHPHEQFTKLGSVGRECVGAAPIRILDEAGNEVPDGEAGELYSSNAHTFDCYWRLPEKTKEAFRGDYCSVGDMAR
RDADGYIHLADRKSNMIISGGENIYPSEVEALVGAHPGVKDIAVVGLPDDKWGERVHAVIVPRDGTTVKECELVDWAKER
LAGFKRPRSYAFITDSEMPRNATGKILHRELKKRFS
>Mature_516_residues
MRDLLNFGQMLSAHARMSPERTGARDLERAMSFSLWNDRACRLANALLGLGLAKGDRVAILAYNCVEWCEIYAATAKAGL
VALPINFRLTAPEVQFIVQNAEASAIIVQDDLIGLVEEARKDLGIAADRFIHFGTKPRPAGYRGYEDIIAQAASREPDQQ
VALSDPWTLMYTSGTTGKPKGVLRSHRSAVLLSMVTEIELKLSRNDGALLVMPMCHANSLNFFGAFGYVGGVNSIYSRKS
FDPEHAVTTLADGGSTFTSLVPTHYIMMLDLPRAIRTQHDFSAVTKLMISSAPARPETKREVMEMFPKSGLYELYGATET
GWVTMLHPHEQFTKLGSVGRECVGAAPIRILDEAGNEVPDGEAGELYSSNAHTFDCYWRLPEKTKEAFRGDYCSVGDMAR
RDADGYIHLADRKSNMIISGGENIYPSEVEALVGAHPGVKDIAVVGLPDDKWGERVHAVIVPRDGTTVKECELVDWAKER
LAGFKRPRSYAFITDSEMPRNATGKILHRELKKRFS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=28.4132841328413, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=28.8201160541586, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI187761345, Length=525, Percent_Identity=28.1904761904762, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI187761343, Length=525, Percent_Identity=28.1904761904762, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI122937307, Length=508, Percent_Identity=27.5590551181102, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI157311624, Length=505, Percent_Identity=25.5445544554455, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI157311622, Length=505, Percent_Identity=25.5445544554455, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI58082049, Length=509, Percent_Identity=25.5402750491159, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI42544132, Length=490, Percent_Identity=27.3469387755102, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI115511026, Length=513, Percent_Identity=26.7056530214425, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI227499619, Length=534, Percent_Identity=23.9700374531835, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI38524616, Length=473, Percent_Identity=24.1014799154334, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI40807357, Length=539, Percent_Identity=22.4489795918367, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI195947378, Length=384, Percent_Identity=22.65625, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788107, Length=526, Percent_Identity=27.9467680608365, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI221142682, Length=494, Percent_Identity=28.1376518218623, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI145693145, Length=522, Percent_Identity=26.0536398467433, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790505, Length=531, Percent_Identity=25.2354048964218, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786810, Length=522, Percent_Identity=22.4137931034483, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786529, Length=555, Percent_Identity=22.7027027027027, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788595, Length=376, Percent_Identity=24.468085106383, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786801, Length=450, Percent_Identity=22.4444444444444, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=514, Percent_Identity=27.8210116731518, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71994694, Length=514, Percent_Identity=27.8210116731518, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71994690, Length=514, Percent_Identity=27.8210116731518, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17560308, Length=507, Percent_Identity=28.0078895463511, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17558820, Length=501, Percent_Identity=30.3393213572854, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17531443, Length=529, Percent_Identity=25.1417769376182, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32563687, Length=510, Percent_Identity=26.8627450980392, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17557194, Length=494, Percent_Identity=27.1255060728745, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=393, Percent_Identity=27.735368956743, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=526, Percent_Identity=26.0456273764259, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17560140, Length=488, Percent_Identity=25.2049180327869, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=25.8953168044077, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17551278, Length=541, Percent_Identity=22.550831792976, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115534440, Length=464, Percent_Identity=22.4137931034483, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=488, Percent_Identity=22.9508196721311, Blast_Score=85, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=29.1666666666667, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24581924, Length=516, Percent_Identity=26.3565891472868, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI21356947, Length=361, Percent_Identity=27.4238227146814, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI18859661, Length=511, Percent_Identity=25.6360078277886, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21355181, Length=532, Percent_Identity=24.0601503759398, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24656500, Length=508, Percent_Identity=22.244094488189, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356441, Length=481, Percent_Identity=24.3243243243243, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24653035, Length=362, Percent_Identity=25.9668508287293, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20130357, Length=385, Percent_Identity=23.8961038961039, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI161076582, Length=369, Percent_Identity=24.1192411924119, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648253, Length=479, Percent_Identity=23.1732776617954, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648255, Length=489, Percent_Identity=22.9038854805726, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI62472339, Length=546, Percent_Identity=24.7252747252747, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24667955, Length=546, Percent_Identity=24.7252747252747, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648260, Length=348, Percent_Identity=22.9885057471264, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24583465, Length=478, Percent_Identity=25.1046025104602, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24583463, Length=478, Percent_Identity=25.1046025104602, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364798, Length=478, Percent_Identity=25.1046025104602, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24667959, Length=379, Percent_Identity=26.9129287598945, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45550496, Length=557, Percent_Identity=22.4416517055655, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI45552801, Length=557, Percent_Identity=22.4416517055655, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281365686, Length=497, Percent_Identity=22.1327967806841, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922652, Length=489, Percent_Identity=21.881390593047, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21358303, Length=476, Percent_Identity=23.109243697479, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573631, Length=552, Percent_Identity=23.3695652173913, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130353, Length=552, Percent_Identity=23.3695652173913, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648257, Length=533, Percent_Identity=21.9512195121951, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 56937; Mature: 56937

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00455 AMP_BINDING ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLLNFGQMLSAHARMSPERTGARDLERAMSFSLWNDRACRLANALLGLGLAKGDRVAI
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEE
LAYNCVEWCEIYAATAKAGLVALPINFRLTAPEVQFIVQNAEASAIIVQDDLIGLVEEAR
EEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHHHH
KDLGIAADRFIHFGTKPRPAGYRGYEDIIAQAASREPDQQVALSDPWTLMYTSGTTGKPK
HHCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCH
GVLRSHRSAVLLSMVTEIELKLSRNDGALLVMPMCHANSLNFFGAFGYVGGVNSIYSRKS
HHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCEEHHHHHCCCHHHHHCCC
FDPEHAVTTLADGGSTFTSLVPTHYIMMLDLPRAIRTQHDFSAVTKLMISSAPARPETKR
CCHHHHHEEECCCCCHHHHHHHHHEEEEEECCHHHHCHHHHHHHHHHHHHCCCCCCHHHH
EVMEMFPKSGLYELYGATETGWVTMLHPHEQFTKLGSVGRECVGAAPIRILDEAGNEVPD
HHHHHCCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GEAGELYSSNAHTFDCYWRLPEKTKEAFRGDYCSVGDMARRDADGYIHLADRKSNMIISG
CCCCCEECCCCCEEEEEEECCHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCCEEEEC
GENIYPSEVEALVGAHPGVKDIAVVGLPDDKWGERVHAVIVPRDGTTVKECELVDWAKER
CCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
LAGFKRPRSYAFITDSEMPRNATGKILHRELKKRFS
HHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRDLLNFGQMLSAHARMSPERTGARDLERAMSFSLWNDRACRLANALLGLGLAKGDRVAI
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEE
LAYNCVEWCEIYAATAKAGLVALPINFRLTAPEVQFIVQNAEASAIIVQDDLIGLVEEAR
EEHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCEEEEEECCCCCEEEEEHHHHHHHHHHH
KDLGIAADRFIHFGTKPRPAGYRGYEDIIAQAASREPDQQVALSDPWTLMYTSGTTGKPK
HHCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCH
GVLRSHRSAVLLSMVTEIELKLSRNDGALLVMPMCHANSLNFFGAFGYVGGVNSIYSRKS
HHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCEEHHHHHCCCHHHHHCCC
FDPEHAVTTLADGGSTFTSLVPTHYIMMLDLPRAIRTQHDFSAVTKLMISSAPARPETKR
CCHHHHHEEECCCCCHHHHHHHHHEEEEEECCHHHHCHHHHHHHHHHHHHCCCCCCHHHH
EVMEMFPKSGLYELYGATETGWVTMLHPHEQFTKLGSVGRECVGAAPIRILDEAGNEVPD
HHHHHCCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCC
GEAGELYSSNAHTFDCYWRLPEKTKEAFRGDYCSVGDMARRDADGYIHLADRKSNMIISG
CCCCCEECCCCCEEEEEEECCHHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCCEEEEC
GENIYPSEVEALVGAHPGVKDIAVVGLPDDKWGERVHAVIVPRDGTTVKECELVDWAKER
CCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
LAGFKRPRSYAFITDSEMPRNATGKILHRELKKRFS
HHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]