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The map label for this gene is hupL [H]

Identifier: 146338751

GI number: 146338751

Start: 1802937

End: 1804727

Strand: Direct

Name: hupL [H]

Synonym: BRADO1685

Alternate gene names: 146338751

Gene position: 1802937-1804727 (Clockwise)

Preceding gene: 146338750

Following gene: 146338752

Centisome position: 24.18

GC content: 62.98

Gene sequence:

>1791_bases
ATGGGCATCCAGACTCCCAACGGCTTCAATCTCGACAATTCCGGTAAGCGCGTCGTCGTCGATCCGCTCACGCGCATCGA
GGGACATCTGCGCGTCGAGGTGAATGTCGACTCCAACAATGTCATCCGCAACGCGGTCTCGACCGGCACGATGTGGCGCG
GCATCGAGACCATCCTGCGCAACCGCGACCCGCGCGACGCCTGGGCTTTCACCGAGCGGATCTGCGGCGTCTGCACCGGC
ACGCATGCGCTGACCTCAGTCCGCGCGGTCGAGAATGCGCTGGGGATCATGATTCCCGACAACGCCAACTCGATCCGCAA
CATCATGCAGCTCTGCCTGCAGGTGCACGACCATCTCGTGCATTTCTATCATCTGCATGCGCTGGACTGGGTCGACGTCG
TCTCGGCGCTGAAGGCCGATCCGAAGGCGACCTCGGCGCTGGCGCAGTCCGTGTCGGACTGGCCGCTGTCCTCGCCGGGC
TACTTCAAGGATCTGCAGATCCGGCTCACCAAGTTCGTCGAGTCAGGCCAGCTTGGCCCGTTCAAGAACGCCTATTGGGG
CCACGCCGCCTACAAGCTGCCGCCGGAGGCGAACCTCATGGCGGTTGCGCATTATCTGGAGGCGCTCGATTTCCAGAAGG
AGATCGTCAAGATCCATACCATCTATGGCGGCAAGAACCCGCATCCGAACTGGCTGGTCGGCGGCGTGCCGTGCGCCGTC
AATGTCGACGGCACCGGGGCGGTCGGTGCCATCAACATGGAGCGGCTCAACCTCGTCTCCTCGATCATCGATCGCTCGAT
CGAGTTCGTGCAGAAGGTCTATCTGCCGGACGTGGTCGCCATCGGCTCGTTCTACAAGGATTGGCTCTATGGTGGCGGCC
TCTCCGGCAAGAGCGTGATGTCGTATGGCGACATTCCGGAGAACGCCAACGACTATTCGGCCAAGAACCTGAAGCTGCCG
CGTGGCGTGATCCTCAACGGCAATCTCAACGAGGTGCTGCCGATCGATCATGGCGATCCCGAGCAGATCCAGGAGTTCGT
GACGCATTCCTGGTACAAATATCCCGACGAGAGCAAGGGTCTGCATCCCTGGGACGGCGTCACCGAGCCGAACTATCAGC
TCGGCCCCAACGCCAAGGGCACGAAGACCGACATCAAGGAGCTCGACGAGGGCGGCAAATATTCCTGGATCAAGGCGCCG
CGCTGGCGCGGCAACGCGGTCGAGGTCGGGCCGCTGGCGCGCTACATCATCGGCTACGCGCAGAACCGGCCGGAGTTCAA
GGAGCCCACGGATAAGCTCCTGAAGACGCTGAACCTGCCGGTCACGGCGCTGTTCTCGACGCTCGGCCGCACCGCGGCGC
GTGCGCTGGAGTGCGACTGGGCCGCGGGGCAGATGCGCTACTTCCAGGACAAGCTGGTCGCGCGCATCAAGGCCGGCGAT
TCCTCGACCGCCAATGTCGAGAAATGGAAGCCGGAGAGCTGGCCCAAGGAAGCCAAGGGCTACGGCTTCACCGAGGCGCC
GCGCGGCGCGCTCGCGCACTGGATCAAGATCAAGGACACCAAGATCGATAATTACCAGTGCGTCGTGCCGACCACCTGGA
ACGGTTCGCCGCGGGATCCCAAGGGCAATATCGGCGCGTTCGAGGCCTCGTTGATGGACACGCCGATGGCGGATCCGGAG
AAGCCGCTCGAGATCCTGCGCACGATCCATTCGTTCGATCCATGCCTGGCGTGCTCGACGCACGTGATTAGCCCGGACGG
CCAGGAAATGGCGACGGTCAAGGTCAGGTAA

Upstream 100 bases:

>100_bases
CGCGGCGCATGCCGCGGTCACCGCCGTCAAGCGGCTCGCGACCAAGCGCGACGACGCCAGCGCCAAGCAAGATCATTAAT
TCAGTTCAGGGCAGGCAACG

Downstream 100 bases:

>100_bases
TGGGAGCAGCACGATGATGGATGCCGTCGCACGTCCCGCGGAGACCGAGACGAACCTCGCCGCGATCGCGGCCGATGCCG
CCGGCGAGCACGCCGTCGGC

Product: uptake hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MGIQTPNGFNLDNSGKRVVVDPLTRIEGHLRVEVNVDSNNVIRNAVSTGTMWRGIETILRNRDPRDAWAFTERICGVCTG
THALTSVRAVENALGIMIPDNANSIRNIMQLCLQVHDHLVHFYHLHALDWVDVVSALKADPKATSALAQSVSDWPLSSPG
YFKDLQIRLTKFVESGQLGPFKNAYWGHAAYKLPPEANLMAVAHYLEALDFQKEIVKIHTIYGGKNPHPNWLVGGVPCAV
NVDGTGAVGAINMERLNLVSSIIDRSIEFVQKVYLPDVVAIGSFYKDWLYGGGLSGKSVMSYGDIPENANDYSAKNLKLP
RGVILNGNLNEVLPIDHGDPEQIQEFVTHSWYKYPDESKGLHPWDGVTEPNYQLGPNAKGTKTDIKELDEGGKYSWIKAP
RWRGNAVEVGPLARYIIGYAQNRPEFKEPTDKLLKTLNLPVTALFSTLGRTAARALECDWAAGQMRYFQDKLVARIKAGD
SSTANVEKWKPESWPKEAKGYGFTEAPRGALAHWIKIKDTKIDNYQCVVPTTWNGSPRDPKGNIGAFEASLMDTPMADPE
KPLEILRTIHSFDPCLACSTHVISPDGQEMATVKVR

Sequences:

>Translated_596_residues
MGIQTPNGFNLDNSGKRVVVDPLTRIEGHLRVEVNVDSNNVIRNAVSTGTMWRGIETILRNRDPRDAWAFTERICGVCTG
THALTSVRAVENALGIMIPDNANSIRNIMQLCLQVHDHLVHFYHLHALDWVDVVSALKADPKATSALAQSVSDWPLSSPG
YFKDLQIRLTKFVESGQLGPFKNAYWGHAAYKLPPEANLMAVAHYLEALDFQKEIVKIHTIYGGKNPHPNWLVGGVPCAV
NVDGTGAVGAINMERLNLVSSIIDRSIEFVQKVYLPDVVAIGSFYKDWLYGGGLSGKSVMSYGDIPENANDYSAKNLKLP
RGVILNGNLNEVLPIDHGDPEQIQEFVTHSWYKYPDESKGLHPWDGVTEPNYQLGPNAKGTKTDIKELDEGGKYSWIKAP
RWRGNAVEVGPLARYIIGYAQNRPEFKEPTDKLLKTLNLPVTALFSTLGRTAARALECDWAAGQMRYFQDKLVARIKAGD
SSTANVEKWKPESWPKEAKGYGFTEAPRGALAHWIKIKDTKIDNYQCVVPTTWNGSPRDPKGNIGAFEASLMDTPMADPE
KPLEILRTIHSFDPCLACSTHVISPDGQEMATVKVR
>Mature_595_residues
GIQTPNGFNLDNSGKRVVVDPLTRIEGHLRVEVNVDSNNVIRNAVSTGTMWRGIETILRNRDPRDAWAFTERICGVCTGT
HALTSVRAVENALGIMIPDNANSIRNIMQLCLQVHDHLVHFYHLHALDWVDVVSALKADPKATSALAQSVSDWPLSSPGY
FKDLQIRLTKFVESGQLGPFKNAYWGHAAYKLPPEANLMAVAHYLEALDFQKEIVKIHTIYGGKNPHPNWLVGGVPCAVN
VDGTGAVGAINMERLNLVSSIIDRSIEFVQKVYLPDVVAIGSFYKDWLYGGGLSGKSVMSYGDIPENANDYSAKNLKLPR
GVILNGNLNEVLPIDHGDPEQIQEFVTHSWYKYPDESKGLHPWDGVTEPNYQLGPNAKGTKTDIKELDEGGKYSWIKAPR
WRGNAVEVGPLARYIIGYAQNRPEFKEPTDKLLKTLNLPVTALFSTLGRTAARALECDWAAGQMRYFQDKLVARIKAGDS
STANVEKWKPESWPKEAKGYGFTEAPRGALAHWIKIKDTKIDNYQCVVPTTWNGSPRDPKGNIGAFEASLMDTPMADPEK
PLEILRTIHSFDPCLACSTHVISPDGQEMATVKVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=590, Percent_Identity=63.3898305084746, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=591, Percent_Identity=46.0236886632826, Blast_Score=506, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66050; Mature: 65919

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIQTPNGFNLDNSGKRVVVDPLTRIEGHLRVEVNVDSNNVIRNAVSTGTMWRGIETILR
CCCCCCCCCCCCCCCCEEEECCHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHC
NRDPRDAWAFTERICGVCTGTHALTSVRAVENALGIMIPDNANSIRNIMQLCLQVHDHLV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVDVVSALKADPKATSALAQSVSDWPLSSPGYFKDLQIRLTKFVESGQLGP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
FKNAYWGHAAYKLPPEANLMAVAHYLEALDFQKEIVKIHTIYGGKNPHPNWLVGGVPCAV
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEECCCEEE
NVDGTGAVGAINMERLNLVSSIIDRSIEFVQKVYLPDVVAIGSFYKDWLYGGGLSGKSVM
ECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHH
SYGDIPENANDYSAKNLKLPRGVILNGNLNEVLPIDHGDPEQIQEFVTHSWYKYPDESKG
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCC
LHPWDGVTEPNYQLGPNAKGTKTDIKELDEGGKYSWIKAPRWRGNAVEVGPLARYIIGYA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHH
QNRPEFKEPTDKLLKTLNLPVTALFSTLGRTAARALECDWAAGQMRYFQDKLVARIKAGD
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
SSTANVEKWKPESWPKEAKGYGFTEAPRGALAHWIKIKDTKIDNYQCVVPTTWNGSPRDP
CCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCCC
KGNIGAFEASLMDTPMADPEKPLEILRTIHSFDPCLACSTHVISPDGQEMATVKVR
CCCCCCEEHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCEEECCCCCCEEEEEEC
>Mature Secondary Structure 
GIQTPNGFNLDNSGKRVVVDPLTRIEGHLRVEVNVDSNNVIRNAVSTGTMWRGIETILR
CCCCCCCCCCCCCCCEEEECCHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHC
NRDPRDAWAFTERICGVCTGTHALTSVRAVENALGIMIPDNANSIRNIMQLCLQVHDHLV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHH
HFYHLHALDWVDVVSALKADPKATSALAQSVSDWPLSSPGYFKDLQIRLTKFVESGQLGP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCC
FKNAYWGHAAYKLPPEANLMAVAHYLEALDFQKEIVKIHTIYGGKNPHPNWLVGGVPCAV
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEECCCEEE
NVDGTGAVGAINMERLNLVSSIIDRSIEFVQKVYLPDVVAIGSFYKDWLYGGGLSGKSVM
ECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHH
SYGDIPENANDYSAKNLKLPRGVILNGNLNEVLPIDHGDPEQIQEFVTHSWYKYPDESKG
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCC
LHPWDGVTEPNYQLGPNAKGTKTDIKELDEGGKYSWIKAPRWRGNAVEVGPLARYIIGYA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHH
QNRPEFKEPTDKLLKTLNLPVTALFSTLGRTAARALECDWAAGQMRYFQDKLVARIKAGD
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
SSTANVEKWKPESWPKEAKGYGFTEAPRGALAHWIKIKDTKIDNYQCVVPTTWNGSPRDP
CCCCCCCCCCCCCCCCHHCCCCCCCCCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCCC
KGNIGAFEASLMDTPMADPEKPLEILRTIHSFDPCLACSTHVISPDGQEMATVKVR
CCCCCCEEHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCEEECCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]